Amino acid dipepetide frequency for Roseospira marina

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.917AlaAla: 18.917 ± 0.171	1.272AlaCys: 1.272 ± 0.032	8.125AlaAsp: 8.125 ± 0.083	7.915AlaGlu: 7.915 ± 0.086	4.239AlaPhe: 4.239 ± 0.069	11.226AlaGly: 11.226 ± 0.109	2.692AlaHis: 2.692 ± 0.05	5.195AlaIle: 5.195 ± 0.065	2.594AlaLys: 2.594 ± 0.053	15.256AlaLeu: 15.256 ± 0.15	3.541AlaMet: 3.541 ± 0.051	2.541AlaAsn: 2.541 ± 0.046	7.055AlaPro: 7.055 ± 0.102	3.643AlaGln: 3.643 ± 0.057	10.924AlaArg: 10.924 ± 0.125	5.586AlaSer: 5.586 ± 0.079	6.814AlaThr: 6.814 ± 0.078	10.496AlaVal: 10.496 ± 0.101	1.899AlaTrp: 1.899 ± 0.041	2.387AlaTyr: 2.387 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	1.079CysAla: 1.079 ± 0.03	0.102CysCys: 0.102 ± 0.01	0.571CysAsp: 0.571 ± 0.022	0.397CysGlu: 0.397 ± 0.017	0.267CysPhe: 0.267 ± 0.015	0.977CysGly: 0.977 ± 0.03	0.274CysHis: 0.274 ± 0.017	0.32CysIle: 0.32 ± 0.015	0.162CysLys: 0.162 ± 0.012	0.912CysLeu: 0.912 ± 0.027	0.156CysMet: 0.156 ± 0.012	0.177CysAsn: 0.177 ± 0.011	0.591CysPro: 0.591 ± 0.02	0.269CysGln: 0.269 ± 0.013	0.708CysArg: 0.708 ± 0.025	0.403CysSer: 0.403 ± 0.019	0.487CysThr: 0.487 ± 0.018	0.715CysVal: 0.715 ± 0.024	0.126CysTrp: 0.126 ± 0.01	0.21CysTyr: 0.21 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	8.375AspAla: 8.375 ± 0.09	0.431AspCys: 0.431 ± 0.018	3.913AspAsp: 3.913 ± 0.079	3.295AspGlu: 3.295 ± 0.052	1.739AspPhe: 1.739 ± 0.039	6.22AspGly: 6.22 ± 0.083	1.462AspHis: 1.462 ± 0.032	2.777AspIle: 2.777 ± 0.047	1.082AspLys: 1.082 ± 0.035	6.851AspLeu: 6.851 ± 0.09	1.442AspMet: 1.442 ± 0.032	1.028AspAsn: 1.028 ± 0.031	4.456AspPro: 4.456 ± 0.064	1.725AspGln: 1.725 ± 0.039	5.279AspArg: 5.279 ± 0.068	2.582AspSer: 2.582 ± 0.046	3.824AspThr: 3.824 ± 0.053	4.746AspVal: 4.746 ± 0.056	0.984AspTrp: 0.984 ± 0.027	1.268AspTyr: 1.268 ± 0.035	0.0AspXaa: 0.0 ± 0.0
Glu	8.152GluAla: 8.152 ± 0.085	0.339GluCys: 0.339 ± 0.015	3.3GluAsp: 3.3 ± 0.052	2.813GluGlu: 2.813 ± 0.059	1.262GluPhe: 1.262 ± 0.033	4.546GluGly: 4.546 ± 0.069	1.282GluHis: 1.282 ± 0.036	2.692GluIle: 2.692 ± 0.054	1.368GluLys: 1.368 ± 0.037	3.981GluLeu: 3.981 ± 0.057	1.405GluMet: 1.405 ± 0.033	1.254GluAsn: 1.254 ± 0.032	2.964GluPro: 2.964 ± 0.064	1.802GluGln: 1.802 ± 0.045	4.942GluArg: 4.942 ± 0.076	2.625GluSer: 2.625 ± 0.047	4.103GluThr: 4.103 ± 0.058	3.918GluVal: 3.918 ± 0.062	0.58GluTrp: 0.58 ± 0.022	0.887GluTyr: 0.887 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	3.737PheAla: 3.737 ± 0.05	0.349PheCys: 0.349 ± 0.015	2.338PheAsp: 2.338 ± 0.043	1.794PheGlu: 1.794 ± 0.039	1.068PhePhe: 1.068 ± 0.031	3.199PheGly: 3.199 ± 0.057	0.727PheHis: 0.727 ± 0.025	1.265PheIle: 1.265 ± 0.032	0.768PheLys: 0.768 ± 0.026	3.102PheLeu: 3.102 ± 0.048	0.713PheMet: 0.713 ± 0.024	0.872PheAsn: 0.872 ± 0.026	1.375PhePro: 1.375 ± 0.036	1.011PheGln: 1.011 ± 0.03	2.132PheArg: 2.132 ± 0.041	1.654PheSer: 1.654 ± 0.038	1.882PheThr: 1.882 ± 0.039	2.448PheVal: 2.448 ± 0.044	0.462PheTrp: 0.462 ± 0.022	0.734PheTyr: 0.734 ± 0.022	0.0PheXaa: 0.0 ± 0.0
Gly	10.697GlyAla: 10.697 ± 0.121	0.945GlyCys: 0.945 ± 0.031	4.954GlyAsp: 4.954 ± 0.061	4.325GlyGlu: 4.325 ± 0.06	3.13GlyPhe: 3.13 ± 0.045	8.14GlyGly: 8.14 ± 0.105	2.149GlyHis: 2.149 ± 0.04	3.797GlyIle: 3.797 ± 0.061	2.184GlyLys: 2.184 ± 0.048	9.921GlyLeu: 9.921 ± 0.102	2.208GlyMet: 2.208 ± 0.048	1.706GlyAsn: 1.706 ± 0.04	4.811GlyPro: 4.811 ± 0.074	2.794GlyGln: 2.794 ± 0.054	7.234GlyArg: 7.234 ± 0.081	4.151GlySer: 4.151 ± 0.065	5.432GlyThr: 5.432 ± 0.065	7.027GlyVal: 7.027 ± 0.077	1.413GlyTrp: 1.413 ± 0.036	2.033GlyTyr: 2.033 ± 0.037	0.0GlyXaa: 0.0 ± 0.0
His	2.787HisAla: 2.787 ± 0.048	0.238HisCys: 0.238 ± 0.014	1.391HisAsp: 1.391 ± 0.038	0.979HisGlu: 0.979 ± 0.028	0.727HisPhe: 0.727 ± 0.024	2.307HisGly: 2.307 ± 0.043	0.628HisHis: 0.628 ± 0.029	0.888HisIle: 0.888 ± 0.026	0.438HisLys: 0.438 ± 0.018	2.217HisLeu: 2.217 ± 0.044	0.532HisMet: 0.532 ± 0.02	0.437HisAsn: 0.437 ± 0.018	1.634HisPro: 1.634 ± 0.038	0.549HisGln: 0.549 ± 0.02	1.676HisArg: 1.676 ± 0.043	0.781HisSer: 0.781 ± 0.027	1.119HisThr: 1.119 ± 0.032	1.692HisVal: 1.692 ± 0.036	0.349HisTrp: 0.349 ± 0.015	0.523HisTyr: 0.523 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	5.912IleAla: 5.912 ± 0.065	0.41IleCys: 0.41 ± 0.017	2.976IleAsp: 2.976 ± 0.05	2.812IleGlu: 2.812 ± 0.045	1.076IlePhe: 1.076 ± 0.03	4.313IleGly: 4.313 ± 0.059	0.896IleHis: 0.896 ± 0.026	1.655IleIle: 1.655 ± 0.041	1.071IleLys: 1.071 ± 0.029	4.233IleLeu: 4.233 ± 0.063	0.838IleMet: 0.838 ± 0.024	1.138IleAsn: 1.138 ± 0.035	2.142IlePro: 2.142 ± 0.042	1.28IleGln: 1.28 ± 0.031	2.947IleArg: 2.947 ± 0.051	2.008IleSer: 2.008 ± 0.045	2.475IleThr: 2.475 ± 0.05	3.513IleVal: 3.513 ± 0.056	0.434IleTrp: 0.434 ± 0.019	0.8IleTyr: 0.8 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	3.296LysAla: 3.296 ± 0.056	0.135LysCys: 0.135 ± 0.01	1.357LysAsp: 1.357 ± 0.033	1.116LysGlu: 1.116 ± 0.036	0.503LysPhe: 0.503 ± 0.02	2.108LysGly: 2.108 ± 0.047	0.44LysHis: 0.44 ± 0.021	0.975LysIle: 0.975 ± 0.032	0.776LysLys: 0.776 ± 0.032	1.809LysLeu: 1.809 ± 0.041	0.535LysMet: 0.535 ± 0.021	0.509LysAsn: 0.509 ± 0.02	1.391LysPro: 1.391 ± 0.034	0.654LysGln: 0.654 ± 0.022	1.667LysArg: 1.667 ± 0.036	1.129LysSer: 1.129 ± 0.035	1.592LysThr: 1.592 ± 0.036	1.726LysVal: 1.726 ± 0.04	0.215LysTrp: 0.215 ± 0.013	0.472LysTyr: 0.472 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	13.973LeuAla: 13.973 ± 0.132	0.979LeuCys: 0.979 ± 0.028	7.156LeuAsp: 7.156 ± 0.08	5.452LeuGlu: 5.452 ± 0.068	3.459LeuPhe: 3.459 ± 0.066	8.836LeuGly: 8.836 ± 0.101	1.989LeuHis: 1.989 ± 0.038	4.408LeuIle: 4.408 ± 0.065	2.519LeuLys: 2.519 ± 0.052	9.183LeuLeu: 9.183 ± 0.126	2.548LeuMet: 2.548 ± 0.045	2.433LeuAsn: 2.433 ± 0.043	5.641LeuPro: 5.641 ± 0.072	2.186LeuGln: 2.186 ± 0.037	7.882LeuArg: 7.882 ± 0.091	5.989LeuSer: 5.989 ± 0.071	6.301LeuThr: 6.301 ± 0.074	7.914LeuVal: 7.914 ± 0.084	1.301LeuTrp: 1.301 ± 0.04	2.0LeuTyr: 2.0 ± 0.046	0.0LeuXaa: 0.0 ± 0.0
Met	3.547MetAla: 3.547 ± 0.052	0.148MetCys: 0.148 ± 0.011	1.402MetAsp: 1.402 ± 0.034	1.209MetGlu: 1.209 ± 0.033	0.616MetPhe: 0.616 ± 0.021	2.038MetGly: 2.038 ± 0.044	0.4MetHis: 0.4 ± 0.016	1.146MetIle: 1.146 ± 0.033	0.696MetLys: 0.696 ± 0.025	2.222MetLeu: 2.222 ± 0.037	0.625MetMet: 0.625 ± 0.023	0.616MetAsn: 0.616 ± 0.02	1.554MetPro: 1.554 ± 0.036	0.625MetGln: 0.625 ± 0.024	1.668MetArg: 1.668 ± 0.041	1.374MetSer: 1.374 ± 0.032	2.08MetThr: 2.08 ± 0.041	1.99MetVal: 1.99 ± 0.039	0.204MetTrp: 0.204 ± 0.012	0.289MetTyr: 0.289 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.93AsnAla: 2.93 ± 0.049	0.188AsnCys: 0.188 ± 0.012	1.256AsnAsp: 1.256 ± 0.035	1.01AsnGlu: 1.01 ± 0.027	0.62AsnPhe: 0.62 ± 0.021	2.02AsnGly: 2.02 ± 0.046	0.446AsnHis: 0.446 ± 0.019	0.986AsnIle: 0.986 ± 0.027	0.457AsnLys: 0.457 ± 0.016	2.055AsnLeu: 2.055 ± 0.043	0.522AsnMet: 0.522 ± 0.021	0.512AsnAsn: 0.512 ± 0.022	1.656AsnPro: 1.656 ± 0.037	0.641AsnGln: 0.641 ± 0.022	1.652AsnArg: 1.652 ± 0.038	0.815AsnSer: 0.815 ± 0.025	1.323AsnThr: 1.323 ± 0.032	1.618AsnVal: 1.618 ± 0.036	0.287AsnTrp: 0.287 ± 0.015	0.452AsnTyr: 0.452 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	7.27ProAla: 7.27 ± 0.109	0.45ProCys: 0.45 ± 0.018	4.958ProAsp: 4.958 ± 0.069	4.144ProGlu: 4.144 ± 0.066	1.904ProPhe: 1.904 ± 0.04	5.303ProGly: 5.303 ± 0.076	1.229ProHis: 1.229 ± 0.028	2.158ProIle: 2.158 ± 0.043	1.305ProLys: 1.305 ± 0.037	5.325ProLeu: 5.325 ± 0.066	1.45ProMet: 1.45 ± 0.032	1.27ProAsn: 1.27 ± 0.035	4.074ProPro: 4.074 ± 0.093	1.317ProGln: 1.317 ± 0.032	3.646ProArg: 3.646 ± 0.062	2.982ProSer: 2.982 ± 0.056	3.215ProThr: 3.215 ± 0.054	4.778ProVal: 4.778 ± 0.063	0.99ProTrp: 0.99 ± 0.028	1.132ProTyr: 1.132 ± 0.028	0.0ProXaa: 0.0 ± 0.0
Gln	4.258GlnAla: 4.258 ± 0.064	0.226GlnCys: 0.226 ± 0.014	1.555GlnAsp: 1.555 ± 0.037	1.336GlnGlu: 1.336 ± 0.036	0.857GlnPhe: 0.857 ± 0.027	2.494GlnGly: 2.494 ± 0.039	0.547GlnHis: 0.547 ± 0.021	1.392GlnIle: 1.392 ± 0.036	0.701GlnLys: 0.701 ± 0.023	1.946GlnLeu: 1.946 ± 0.042	0.692GlnMet: 0.692 ± 0.024	0.653GlnAsn: 0.653 ± 0.024	1.591GlnPro: 1.591 ± 0.038	0.867GlnGln: 0.867 ± 0.032	2.324GlnArg: 2.324 ± 0.047	1.348GlnSer: 1.348 ± 0.033	1.92GlnThr: 1.92 ± 0.043	2.271GlnVal: 2.271 ± 0.041	0.358GlnTrp: 0.358 ± 0.017	0.509GlnTyr: 0.509 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	10.16ArgAla: 10.16 ± 0.114	0.642ArgCys: 0.642 ± 0.024	4.952ArgAsp: 4.952 ± 0.066	4.023ArgGlu: 4.023 ± 0.063	2.722ArgPhe: 2.722 ± 0.043	5.286ArgGly: 5.286 ± 0.066	2.098ArgHis: 2.098 ± 0.049	3.572ArgIle: 3.572 ± 0.057	1.711ArgLys: 1.711 ± 0.036	8.981ArgLeu: 8.981 ± 0.107	1.979ArgMet: 1.979 ± 0.039	1.54ArgAsn: 1.54 ± 0.03	4.768ArgPro: 4.768 ± 0.073	2.428ArgGln: 2.428 ± 0.045	7.013ArgArg: 7.013 ± 0.104	3.353ArgSer: 3.353 ± 0.055	4.215ArgThr: 4.215 ± 0.064	6.034ArgVal: 6.034 ± 0.088	1.065ArgTrp: 1.065 ± 0.03	1.55ArgTyr: 1.55 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	5.797SerAla: 5.797 ± 0.07	0.374SerCys: 0.374 ± 0.015	2.825SerAsp: 2.825 ± 0.049	2.404SerGlu: 2.404 ± 0.05	1.552SerPhe: 1.552 ± 0.034	5.17SerGly: 5.17 ± 0.077	1.037SerHis: 1.037 ± 0.029	2.026SerIle: 2.026 ± 0.045	1.023SerLys: 1.023 ± 0.032	4.926SerLeu: 4.926 ± 0.061	1.247SerMet: 1.247 ± 0.03	1.012SerAsn: 1.012 ± 0.03	2.718SerPro: 2.718 ± 0.055	1.371SerGln: 1.371 ± 0.035	3.462SerArg: 3.462 ± 0.054	2.262SerSer: 2.262 ± 0.05	2.561SerThr: 2.561 ± 0.042	3.849SerVal: 3.849 ± 0.059	0.612SerTrp: 0.612 ± 0.023	1.034SerTyr: 1.034 ± 0.026	0.0SerXaa: 0.0 ± 0.0
Thr	7.554ThrAla: 7.554 ± 0.082	0.505ThrCys: 0.505 ± 0.021	3.649ThrAsp: 3.649 ± 0.058	3.094ThrGlu: 3.094 ± 0.048	1.905ThrPhe: 1.905 ± 0.043	5.764ThrGly: 5.764 ± 0.078	1.213ThrHis: 1.213 ± 0.033	2.595ThrIle: 2.595 ± 0.053	1.126ThrLys: 1.126 ± 0.034	7.049ThrLeu: 7.049 ± 0.079	1.236ThrMet: 1.236 ± 0.031	1.195ThrAsn: 1.195 ± 0.036	4.136ThrPro: 4.136 ± 0.058	1.531ThrGln: 1.531 ± 0.034	4.034ThrArg: 4.034 ± 0.062	2.413ThrSer: 2.413 ± 0.046	3.294ThrThr: 3.294 ± 0.056	5.592ThrVal: 5.592 ± 0.08	0.801ThrTrp: 0.801 ± 0.026	1.166ThrTyr: 1.166 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	10.147ValAla: 10.147 ± 0.1	0.795ValCys: 0.795 ± 0.027	4.665ValAsp: 4.665 ± 0.061	4.538ValGlu: 4.538 ± 0.059	2.838ValPhe: 2.838 ± 0.053	6.212ValGly: 6.212 ± 0.08	1.561ValHis: 1.561 ± 0.039	3.658ValIle: 3.658 ± 0.058	1.665ValLys: 1.665 ± 0.043	8.599ValLeu: 8.599 ± 0.088	1.934ValMet: 1.934 ± 0.035	1.751ValAsn: 1.751 ± 0.036	4.579ValPro: 4.579 ± 0.063	2.052ValGln: 2.052 ± 0.042	5.924ValArg: 5.924 ± 0.072	4.133ValSer: 4.133 ± 0.055	5.233ValThr: 5.233 ± 0.074	6.926ValVal: 6.926 ± 0.079	1.02ValTrp: 1.02 ± 0.031	1.519ValTyr: 1.519 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	1.385TrpAla: 1.385 ± 0.031	0.15TrpCys: 0.15 ± 0.011	0.769TrpAsp: 0.769 ± 0.026	0.546TrpGlu: 0.546 ± 0.022	0.457TrpPhe: 0.457 ± 0.019	1.015TrpGly: 1.015 ± 0.032	0.343TrpHis: 0.343 ± 0.015	0.639TrpIle: 0.639 ± 0.023	0.273TrpLys: 0.273 ± 0.015	1.587TrpLeu: 1.587 ± 0.04	0.383TrpMet: 0.383 ± 0.019	0.331TrpAsn: 0.331 ± 0.016	0.79TrpPro: 0.79 ± 0.027	0.454TrpGln: 0.454 ± 0.02	1.358TrpArg: 1.358 ± 0.036	0.784TrpSer: 0.784 ± 0.026	0.888TrpThr: 0.888 ± 0.027	0.99TrpVal: 0.99 ± 0.03	0.236TrpTrp: 0.236 ± 0.011	0.272TrpTyr: 0.272 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.344TyrAla: 2.344 ± 0.039	0.223TyrCys: 0.223 ± 0.014	1.367TyrAsp: 1.367 ± 0.033	1.013TyrGlu: 1.013 ± 0.03	0.692TyrPhe: 0.692 ± 0.024	1.88TyrGly: 1.88 ± 0.039	0.479TyrHis: 0.479 ± 0.018	0.782TyrIle: 0.782 ± 0.026	0.484TyrLys: 0.484 ± 0.018	1.996TyrLeu: 1.996 ± 0.043	0.461TyrMet: 0.461 ± 0.018	0.471TyrAsn: 0.471 ± 0.02	0.974TyrPro: 0.974 ± 0.026	0.645TyrGln: 0.645 ± 0.024	1.621TyrArg: 1.621 ± 0.035	0.892TyrSer: 0.892 ± 0.025	1.098TyrThr: 1.098 ± 0.036	1.506TyrVal: 1.506 ± 0.038	0.311TyrTrp: 0.311 ± 0.015	0.538TyrTyr: 0.538 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4009 proteins (1330878 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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