Gordonia phage Stormageddon

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 213 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A649VR46|A0A649VR46_9CAUD Uncharacterized protein OS=Gordonia phage Stormageddon OX=2656541 GN=117 PE=4 SV=1
MM1 pKa = 7.41TSYY4 pKa = 11.26LKK6 pKa = 10.85LSEE9 pKa = 4.23RR10 pKa = 11.84PGLSFTAPEE19 pKa = 4.29CNACLKK25 pKa = 10.59EE26 pKa = 3.88VDD28 pKa = 3.94HH29 pKa = 7.56DD30 pKa = 4.58GDD32 pKa = 3.43GWLCPSCGTSWPGNVMEE49 pKa = 5.28SDD51 pKa = 3.62GSDD54 pKa = 2.95AQLFEE59 pKa = 4.42EE60 pKa = 4.86WSGEE64 pKa = 4.02EE65 pKa = 5.08LPGPTCKK72 pKa = 9.66TDD74 pKa = 3.29EE75 pKa = 4.93AYY77 pKa = 10.67LAAISPSDD85 pKa = 2.73WWKK88 pKa = 9.85RR89 pKa = 11.84WPSCVYY95 pKa = 10.1NQSDD99 pKa = 5.1DD100 pKa = 5.15DD101 pKa = 4.69HH102 pKa = 7.14DD103 pKa = 3.9QQKK106 pKa = 11.07DD107 pKa = 3.68GNGG110 pKa = 3.23

Molecular weight: 12.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A649VRP1|A0A649VRP1_9CAUD Terminase OS=Gordonia phage Stormageddon OX=2656541 GN=1 PE=4 SV=1
MM1 pKa = 7.25WVKK4 pKa = 10.46KK5 pKa = 10.6APEE8 pKa = 4.4APAHH12 pKa = 6.28ACDD15 pKa = 4.04KK16 pKa = 10.86PMARR20 pKa = 11.84LMVRR24 pKa = 11.84PPRR27 pKa = 11.84TNGRR31 pKa = 11.84GEE33 pKa = 3.9VVMPVRR39 pKa = 11.84PKK41 pKa = 10.81VPVGPPIPDD50 pKa = 3.34GKK52 pKa = 11.29VGDD55 pKa = 3.48VWLCDD60 pKa = 3.2EE61 pKa = 5.42CLTVWKK67 pKa = 9.96VGQEE71 pKa = 3.88IGPRR75 pKa = 11.84TYY77 pKa = 10.44PYY79 pKa = 10.2TPGALRR85 pKa = 11.84PVWRR89 pKa = 11.84RR90 pKa = 11.84AGWWTTRR97 pKa = 11.84KK98 pKa = 9.66ARR100 pKa = 11.84RR101 pKa = 11.84AVGTAA106 pKa = 2.95

Molecular weight: 11.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

213

0

213

43589

25

1149

204.6

22.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.661 ± 0.265

0.95 ± 0.072

7.094 ± 0.203

6.809 ± 0.25

2.916 ± 0.119

7.899 ± 0.259

2.106 ± 0.107

4.249 ± 0.12

3.682 ± 0.149

7.575 ± 0.196

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.478 ± 0.103

3.375 ± 0.136

5.57 ± 0.163

3.728 ± 0.133

6.722 ± 0.192

6.318 ± 0.173

6.405 ± 0.249

7.387 ± 0.181

1.845 ± 0.108

3.232 ± 0.13
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski