Amino acid dipepetide frequency for Gordonia phage Stormageddon

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.769AlaAla: 11.769 ± 0.891
0.642AlaCys: 0.642 ± 0.119
6.814AlaAsp: 6.814 ± 0.464
7.112AlaGlu: 7.112 ± 0.503
2.73AlaPhe: 2.73 ± 0.262
6.86AlaGly: 6.86 ± 0.433
1.904AlaHis: 1.904 ± 0.21
4.175AlaIle: 4.175 ± 0.354
3.854AlaLys: 3.854 ± 0.3
8.03AlaLeu: 8.03 ± 0.564
2.386AlaMet: 2.386 ± 0.274
3.464AlaAsn: 3.464 ± 0.325
4.68AlaPro: 4.68 ± 0.419
4.198AlaGln: 4.198 ± 0.376
6.355AlaArg: 6.355 ± 0.394
5.85AlaSer: 5.85 ± 0.443
5.575AlaThr: 5.575 ± 0.452
6.378AlaVal: 6.378 ± 0.385
1.537AlaTrp: 1.537 ± 0.205
2.294AlaTyr: 2.294 ± 0.201
0.0AlaXaa: 0.0 ± 0.0
Cys
0.619CysAla: 0.619 ± 0.131
0.092CysCys: 0.092 ± 0.053
0.734CysAsp: 0.734 ± 0.122
0.482CysGlu: 0.482 ± 0.096
0.184CysPhe: 0.184 ± 0.069
1.331CysGly: 1.331 ± 0.222
0.229CysHis: 0.229 ± 0.081
0.298CysIle: 0.298 ± 0.094
0.321CysLys: 0.321 ± 0.084
0.596CysLeu: 0.596 ± 0.112
0.206CysMet: 0.206 ± 0.076
0.436CysAsn: 0.436 ± 0.12
0.665CysPro: 0.665 ± 0.133
0.229CysGln: 0.229 ± 0.082
0.665CysArg: 0.665 ± 0.121
0.665CysSer: 0.665 ± 0.162
0.528CysThr: 0.528 ± 0.11
0.482CysVal: 0.482 ± 0.112
0.206CysTrp: 0.206 ± 0.069
0.528CysTyr: 0.528 ± 0.107
0.0CysXaa: 0.0 ± 0.0
Asp
6.47AspAla: 6.47 ± 0.407
0.665AspCys: 0.665 ± 0.135
5.575AspAsp: 5.575 ± 0.579
5.552AspGlu: 5.552 ± 0.478
2.134AspPhe: 2.134 ± 0.211
5.758AspGly: 5.758 ± 0.391
1.629AspHis: 1.629 ± 0.209
3.763AspIle: 3.763 ± 0.319
1.744AspLys: 1.744 ± 0.188
5.804AspLeu: 5.804 ± 0.43
1.377AspMet: 1.377 ± 0.187
1.927AspAsn: 1.927 ± 0.21
5.552AspPro: 5.552 ± 0.347
2.111AspGln: 2.111 ± 0.226
4.772AspArg: 4.772 ± 0.341
3.143AspSer: 3.143 ± 0.375
3.946AspThr: 3.946 ± 0.317
5.001AspVal: 5.001 ± 0.337
1.422AspTrp: 1.422 ± 0.244
2.592AspTyr: 2.592 ± 0.258
0.0AspXaa: 0.0 ± 0.0
Glu
6.906GluAla: 6.906 ± 0.517
0.734GluCys: 0.734 ± 0.141
4.474GluAsp: 4.474 ± 0.468
4.818GluGlu: 4.818 ± 0.493
2.455GluPhe: 2.455 ± 0.226
4.543GluGly: 4.543 ± 0.308
1.377GluHis: 1.377 ± 0.182
3.189GluIle: 3.189 ± 0.262
2.524GluLys: 2.524 ± 0.201
6.034GluLeu: 6.034 ± 0.391
1.927GluMet: 1.927 ± 0.223
1.858GluAsn: 1.858 ± 0.249
3.166GluPro: 3.166 ± 0.301
2.822GluGln: 2.822 ± 0.267
5.047GluArg: 5.047 ± 0.379
3.212GluSer: 3.212 ± 0.308
3.51GluThr: 3.51 ± 0.338
5.736GluVal: 5.736 ± 0.369
1.744GluTrp: 1.744 ± 0.229
2.019GluTyr: 2.019 ± 0.246
0.0GluXaa: 0.0 ± 0.0
Phe
2.753PheAla: 2.753 ± 0.306
0.275PheCys: 0.275 ± 0.071
2.638PheAsp: 2.638 ± 0.268
2.179PheGlu: 2.179 ± 0.214
0.849PhePhe: 0.849 ± 0.156
2.524PheGly: 2.524 ± 0.245
0.551PheHis: 0.551 ± 0.116
1.124PheIle: 1.124 ± 0.156
0.941PheLys: 0.941 ± 0.169
1.812PheLeu: 1.812 ± 0.213
0.872PheMet: 0.872 ± 0.142
0.826PheAsn: 0.826 ± 0.127
1.767PhePro: 1.767 ± 0.221
0.872PheGln: 0.872 ± 0.144
1.835PheArg: 1.835 ± 0.204
2.019PheSer: 2.019 ± 0.225
2.111PheThr: 2.111 ± 0.292
1.904PheVal: 1.904 ± 0.206
0.298PheTrp: 0.298 ± 0.082
1.009PheTyr: 1.009 ± 0.172
0.0PheXaa: 0.0 ± 0.0
Gly
6.171GlyAla: 6.171 ± 0.429
0.849GlyCys: 0.849 ± 0.162
5.621GlyAsp: 5.621 ± 0.298
4.795GlyGlu: 4.795 ± 0.347
2.501GlyPhe: 2.501 ± 0.249
7.364GlyGly: 7.364 ± 0.923
1.789GlyHis: 1.789 ± 0.211
3.212GlyIle: 3.212 ± 0.285
3.418GlyLys: 3.418 ± 0.306
5.736GlyLeu: 5.736 ± 0.369
2.432GlyMet: 2.432 ± 0.244
2.432GlyAsn: 2.432 ± 0.3
3.258GlyPro: 3.258 ± 0.262
2.914GlyGln: 2.914 ± 0.26
5.047GlyArg: 5.047 ± 0.315
5.185GlySer: 5.185 ± 0.43
6.034GlyThr: 6.034 ± 0.621
5.07GlyVal: 5.07 ± 0.318
2.065GlyTrp: 2.065 ± 0.249
3.097GlyTyr: 3.097 ± 0.269
0.0GlyXaa: 0.0 ± 0.0
His
1.904HisAla: 1.904 ± 0.243
0.206HisCys: 0.206 ± 0.071
1.354HisAsp: 1.354 ± 0.18
1.17HisGlu: 1.17 ± 0.211
0.895HisPhe: 0.895 ± 0.152
1.422HisGly: 1.422 ± 0.191
0.528HisHis: 0.528 ± 0.126
0.711HisIle: 0.711 ± 0.143
0.619HisLys: 0.619 ± 0.128
1.491HisLeu: 1.491 ± 0.191
0.39HisMet: 0.39 ± 0.104
0.803HisAsn: 0.803 ± 0.149
1.652HisPro: 1.652 ± 0.246
0.918HisGln: 0.918 ± 0.14
1.721HisArg: 1.721 ± 0.238
1.354HisSer: 1.354 ± 0.172
1.17HisThr: 1.17 ± 0.19
1.606HisVal: 1.606 ± 0.211
0.482HisTrp: 0.482 ± 0.102
0.665HisTyr: 0.665 ± 0.116
0.0HisXaa: 0.0 ± 0.0
Ile
4.153IleAla: 4.153 ± 0.283
0.459IleCys: 0.459 ± 0.096
3.602IleAsp: 3.602 ± 0.322
3.785IleGlu: 3.785 ± 0.276
0.78IlePhe: 0.78 ± 0.123
3.717IleGly: 3.717 ± 0.31
0.78IleHis: 0.78 ± 0.139
1.583IleIle: 1.583 ± 0.185
1.583IleLys: 1.583 ± 0.218
2.478IleLeu: 2.478 ± 0.264
0.872IleMet: 0.872 ± 0.141
1.583IleAsn: 1.583 ± 0.247
2.386IlePro: 2.386 ± 0.247
1.744IleGln: 1.744 ± 0.227
2.547IleArg: 2.547 ± 0.239
2.822IleSer: 2.822 ± 0.355
2.937IleThr: 2.937 ± 0.296
2.891IleVal: 2.891 ± 0.286
0.642IleTrp: 0.642 ± 0.115
1.147IleTyr: 1.147 ± 0.147
0.0IleXaa: 0.0 ± 0.0
Lys
3.992LysAla: 3.992 ± 0.313
0.344LysCys: 0.344 ± 0.093
2.179LysAsp: 2.179 ± 0.234
2.179LysGlu: 2.179 ± 0.249
0.78LysPhe: 0.78 ± 0.169
3.074LysGly: 3.074 ± 0.305
0.78LysHis: 0.78 ± 0.153
1.262LysIle: 1.262 ± 0.173
1.835LysLys: 1.835 ± 0.284
2.891LysLeu: 2.891 ± 0.231
0.987LysMet: 0.987 ± 0.163
1.147LysAsn: 1.147 ± 0.149
2.294LysPro: 2.294 ± 0.231
1.147LysGln: 1.147 ± 0.203
2.501LysArg: 2.501 ± 0.238
2.271LysSer: 2.271 ± 0.242
2.111LysThr: 2.111 ± 0.231
3.327LysVal: 3.327 ± 0.32
0.596LysTrp: 0.596 ± 0.138
1.124LysTyr: 1.124 ± 0.154
0.0LysXaa: 0.0 ± 0.0
Leu
7.364LeuAla: 7.364 ± 0.532
0.826LeuCys: 0.826 ± 0.13
6.08LeuAsp: 6.08 ± 0.382
4.038LeuGlu: 4.038 ± 0.27
2.134LeuPhe: 2.134 ± 0.224
5.781LeuGly: 5.781 ± 0.331
1.537LeuHis: 1.537 ± 0.206
3.212LeuIle: 3.212 ± 0.241
2.799LeuLys: 2.799 ± 0.26
4.933LeuLeu: 4.933 ± 0.456
1.858LeuMet: 1.858 ± 0.212
2.914LeuAsn: 2.914 ± 0.276
3.854LeuPro: 3.854 ± 0.361
3.005LeuGln: 3.005 ± 0.234
6.378LeuArg: 6.378 ± 0.402
5.644LeuSer: 5.644 ± 0.412
5.093LeuThr: 5.093 ± 0.36
5.437LeuVal: 5.437 ± 0.348
0.78LeuTrp: 0.78 ± 0.153
2.088LeuTyr: 2.088 ± 0.25
0.0LeuXaa: 0.0 ± 0.0
Met
2.386MetAla: 2.386 ± 0.272
0.252MetCys: 0.252 ± 0.078
1.56MetAsp: 1.56 ± 0.216
1.606MetGlu: 1.606 ± 0.194
0.711MetPhe: 0.711 ± 0.13
1.491MetGly: 1.491 ± 0.159
0.459MetHis: 0.459 ± 0.104
1.055MetIle: 1.055 ± 0.162
0.964MetLys: 0.964 ± 0.146
1.491MetLeu: 1.491 ± 0.196
0.596MetMet: 0.596 ± 0.13
1.078MetAsn: 1.078 ± 0.176
1.399MetPro: 1.399 ± 0.196
0.803MetGln: 0.803 ± 0.135
1.95MetArg: 1.95 ± 0.209
2.157MetSer: 2.157 ± 0.218
2.042MetThr: 2.042 ± 0.237
1.629MetVal: 1.629 ± 0.22
0.39MetTrp: 0.39 ± 0.099
0.757MetTyr: 0.757 ± 0.141
0.0MetXaa: 0.0 ± 0.0
Asn
3.189AsnAla: 3.189 ± 0.289
0.367AsnCys: 0.367 ± 0.115
2.34AsnAsp: 2.34 ± 0.284
1.698AsnGlu: 1.698 ± 0.241
1.055AsnPhe: 1.055 ± 0.162
3.441AsnGly: 3.441 ± 0.351
0.551AsnHis: 0.551 ± 0.109
1.147AsnIle: 1.147 ± 0.139
1.101AsnLys: 1.101 ± 0.143
2.914AsnLeu: 2.914 ± 0.268
0.436AsnMet: 0.436 ± 0.097
0.987AsnAsn: 0.987 ± 0.135
2.753AsnPro: 2.753 ± 0.268
1.399AsnGln: 1.399 ± 0.229
2.202AsnArg: 2.202 ± 0.206
2.111AsnSer: 2.111 ± 0.262
2.042AsnThr: 2.042 ± 0.235
2.111AsnVal: 2.111 ± 0.191
0.757AsnTrp: 0.757 ± 0.132
1.147AsnTyr: 1.147 ± 0.182
0.0AsnXaa: 0.0 ± 0.0
Pro
4.978ProAla: 4.978 ± 0.422
0.459ProCys: 0.459 ± 0.103
4.818ProAsp: 4.818 ± 0.353
5.07ProGlu: 5.07 ± 0.375
1.422ProPhe: 1.422 ± 0.173
4.543ProGly: 4.543 ± 0.411
1.055ProHis: 1.055 ± 0.162
2.248ProIle: 2.248 ± 0.254
1.996ProLys: 1.996 ± 0.221
3.671ProLeu: 3.671 ± 0.271
1.055ProMet: 1.055 ± 0.129
2.317ProAsn: 2.317 ± 0.216
2.776ProPro: 2.776 ± 0.354
1.858ProGln: 1.858 ± 0.23
2.776ProArg: 2.776 ± 0.291
3.74ProSer: 3.74 ± 0.332
3.831ProThr: 3.831 ± 0.448
4.703ProVal: 4.703 ± 0.366
0.734ProTrp: 0.734 ± 0.135
1.652ProTyr: 1.652 ± 0.215
0.0ProXaa: 0.0 ± 0.0
Gln
4.221GlnAla: 4.221 ± 0.345
0.206GlnCys: 0.206 ± 0.066
2.019GlnAsp: 2.019 ± 0.204
2.179GlnGlu: 2.179 ± 0.219
1.491GlnPhe: 1.491 ± 0.226
2.202GlnGly: 2.202 ± 0.236
0.757GlnHis: 0.757 ± 0.147
1.537GlnIle: 1.537 ± 0.164
1.399GlnLys: 1.399 ± 0.156
3.418GlnLeu: 3.418 ± 0.286
1.147GlnMet: 1.147 ± 0.152
1.124GlnAsn: 1.124 ± 0.201
1.996GlnPro: 1.996 ± 0.215
1.468GlnGln: 1.468 ± 0.209
3.074GlnArg: 3.074 ± 0.311
2.271GlnSer: 2.271 ± 0.26
2.065GlnThr: 2.065 ± 0.22
2.707GlnVal: 2.707 ± 0.244
0.987GlnTrp: 0.987 ± 0.163
1.009GlnTyr: 1.009 ± 0.156
0.0GlnXaa: 0.0 ± 0.0
Arg
5.804ArgAla: 5.804 ± 0.408
0.803ArgCys: 0.803 ± 0.165
4.084ArgAsp: 4.084 ± 0.342
4.749ArgGlu: 4.749 ± 0.409
1.95ArgPhe: 1.95 ± 0.262
4.359ArgGly: 4.359 ± 0.338
2.179ArgHis: 2.179 ± 0.231
2.937ArgIle: 2.937 ± 0.275
3.212ArgLys: 3.212 ± 0.314
5.3ArgLeu: 5.3 ± 0.369
1.721ArgMet: 1.721 ± 0.213
2.065ArgAsn: 2.065 ± 0.198
3.418ArgPro: 3.418 ± 0.315
2.707ArgGln: 2.707 ± 0.329
4.749ArgArg: 4.749 ± 0.413
4.015ArgSer: 4.015 ± 0.378
3.923ArgThr: 3.923 ± 0.296
5.368ArgVal: 5.368 ± 0.397
1.285ArgTrp: 1.285 ± 0.205
2.592ArgTyr: 2.592 ± 0.259
0.0ArgXaa: 0.0 ± 0.0
Ser
5.873SerAla: 5.873 ± 0.373
0.619SerCys: 0.619 ± 0.126
4.198SerAsp: 4.198 ± 0.387
4.382SerGlu: 4.382 ± 0.403
1.537SerPhe: 1.537 ± 0.183
5.988SerGly: 5.988 ± 0.405
1.239SerHis: 1.239 ± 0.211
2.776SerIle: 2.776 ± 0.233
2.478SerLys: 2.478 ± 0.223
4.198SerLeu: 4.198 ± 0.289
1.904SerMet: 1.904 ± 0.209
2.179SerAsn: 2.179 ± 0.282
3.327SerPro: 3.327 ± 0.264
2.386SerGln: 2.386 ± 0.275
3.464SerArg: 3.464 ± 0.301
4.405SerSer: 4.405 ± 0.413
4.061SerThr: 4.061 ± 0.368
4.933SerVal: 4.933 ± 0.341
1.17SerTrp: 1.17 ± 0.132
2.065SerTyr: 2.065 ± 0.23
0.0SerXaa: 0.0 ± 0.0
Thr
6.791ThrAla: 6.791 ± 0.54
0.482ThrCys: 0.482 ± 0.117
4.038ThrAsp: 4.038 ± 0.352
4.015ThrGlu: 4.015 ± 0.34
2.524ThrPhe: 2.524 ± 0.242
5.047ThrGly: 5.047 ± 0.4
1.262ThrHis: 1.262 ± 0.202
2.868ThrIle: 2.868 ± 0.265
1.927ThrLys: 1.927 ± 0.26
4.497ThrLeu: 4.497 ± 0.317
1.399ThrMet: 1.399 ± 0.164
2.042ThrAsn: 2.042 ± 0.232
4.795ThrPro: 4.795 ± 0.44
1.973ThrGln: 1.973 ± 0.224
3.212ThrArg: 3.212 ± 0.239
3.74ThrSer: 3.74 ± 0.399
4.978ThrThr: 4.978 ± 0.593
4.955ThrVal: 4.955 ± 0.352
0.987ThrTrp: 0.987 ± 0.16
2.524ThrTyr: 2.524 ± 0.292
0.0ThrXaa: 0.0 ± 0.0
Val
7.112ValAla: 7.112 ± 0.468
0.757ValCys: 0.757 ± 0.154
4.795ValAsp: 4.795 ± 0.352
5.323ValGlu: 5.323 ± 0.37
1.789ValPhe: 1.789 ± 0.206
5.3ValGly: 5.3 ± 0.392
1.468ValHis: 1.468 ± 0.218
3.51ValIle: 3.51 ± 0.284
2.363ValLys: 2.363 ± 0.226
6.011ValLeu: 6.011 ± 0.418
1.812ValMet: 1.812 ± 0.206
2.776ValAsn: 2.776 ± 0.281
3.717ValPro: 3.717 ± 0.349
2.478ValGln: 2.478 ± 0.248
5.437ValArg: 5.437 ± 0.371
5.001ValSer: 5.001 ± 0.377
4.91ValThr: 4.91 ± 0.321
5.598ValVal: 5.598 ± 0.364
1.354ValTrp: 1.354 ± 0.185
2.363ValTyr: 2.363 ± 0.266
0.0ValXaa: 0.0 ± 0.0
Trp
1.675TrpAla: 1.675 ± 0.201
0.115TrpCys: 0.115 ± 0.049
1.445TrpAsp: 1.445 ± 0.227
0.826TrpGlu: 0.826 ± 0.162
0.436TrpPhe: 0.436 ± 0.115
1.101TrpGly: 1.101 ± 0.198
0.321TrpHis: 0.321 ± 0.091
0.619TrpIle: 0.619 ± 0.114
0.757TrpLys: 0.757 ± 0.152
1.583TrpLeu: 1.583 ± 0.216
0.596TrpMet: 0.596 ± 0.107
0.734TrpAsn: 0.734 ± 0.125
0.826TrpPro: 0.826 ± 0.154
0.826TrpGln: 0.826 ± 0.148
1.399TrpArg: 1.399 ± 0.213
1.422TrpSer: 1.422 ± 0.172
1.399TrpThr: 1.399 ± 0.16
1.239TrpVal: 1.239 ± 0.169
0.528TrpTrp: 0.528 ± 0.124
0.596TrpTyr: 0.596 ± 0.128
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.478TyrAla: 2.478 ± 0.265
0.344TyrCys: 0.344 ± 0.105
2.57TyrAsp: 2.57 ± 0.222
2.134TyrGlu: 2.134 ± 0.208
0.803TyrPhe: 0.803 ± 0.152
3.143TyrGly: 3.143 ± 0.309
0.665TyrHis: 0.665 ± 0.107
1.262TyrIle: 1.262 ± 0.185
0.987TyrLys: 0.987 ± 0.185
2.868TyrLeu: 2.868 ± 0.263
0.78TyrMet: 0.78 ± 0.164
1.055TyrAsn: 1.055 ± 0.148
1.422TyrPro: 1.422 ± 0.222
1.422TyrGln: 1.422 ± 0.168
2.088TyrArg: 2.088 ± 0.239
2.157TyrSer: 2.157 ± 0.258
1.789TyrThr: 1.789 ± 0.233
2.799TyrVal: 2.799 ± 0.236
0.482TyrTrp: 0.482 ± 0.094
1.055TyrTyr: 1.055 ± 0.171
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 213 proteins (43589 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski