Acidianus filamentous virus 7
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A7WKM6|A7WKM6_9VIRU Uncharacterized protein OS=Acidianus filamentous virus 7 OX=346883 PE=4 SV=1
MM1 pKa = 7.51 RR2 pKa = 11.84 VFVHH6 pKa = 5.77 VEE8 pKa = 3.83 GVVDD12 pKa = 4.76 VEE14 pKa = 4.48 EE15 pKa = 4.51 SCNDD19 pKa = 3.32 EE20 pKa = 4.72 LEE22 pKa = 4.37 CLLIYY27 pKa = 10.74 SEE29 pKa = 4.3 MLEE32 pKa = 4.29 KK33 pKa = 11.19 YY34 pKa = 10.17 GDD36 pKa = 3.77 EE37 pKa = 4.58 LGYY40 pKa = 10.88 CYY42 pKa = 10.61 GLSLQEE48 pKa = 4.14 RR49 pKa = 11.84 VKK51 pKa = 10.77 CSHH54 pKa = 6.68 GYY56 pKa = 9.89 VEE58 pKa = 4.89 IKK60 pKa = 10.42 RR61 pKa = 3.87
Molecular weight: 7.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.477
IPC2_protein 4.673
IPC_protein 4.495
Toseland 4.368
ProMoST 4.584
Dawson 4.418
Bjellqvist 4.571
Wikipedia 4.266
Rodwell 4.342
Grimsley 4.291
Solomon 4.418
Lehninger 4.368
Nozaki 4.533
DTASelect 4.609
Thurlkill 4.368
EMBOSS 4.279
Sillero 4.609
Patrickios 0.655
IPC_peptide 4.431
IPC2_peptide 4.596
IPC2.peptide.svr19 4.551
Protein with the highest isoelectric point:
>tr|A7WKP9|A7WKP9_9VIRU Uncharacterized protein OS=Acidianus filamentous virus 7 OX=346883 PE=4 SV=1
MM1 pKa = 7.54 TIKK4 pKa = 9.63 KK5 pKa = 5.83 TTFIRR10 pKa = 11.84 LCFWTLSQARR20 pKa = 11.84 MSGIKK25 pKa = 6.81 TTPKK29 pKa = 9.97 IMLYY33 pKa = 10.46 FLDD36 pKa = 4.06 EE37 pKa = 4.55 VKK39 pKa = 10.77 VSPRR43 pKa = 11.84 TKK45 pKa = 10.42 EE46 pKa = 3.82 RR47 pKa = 11.84 LLLLIDD53 pKa = 4.24 EE54 pKa = 5.49 LDD56 pKa = 3.53 TGKK59 pKa = 8.47 ITPRR63 pKa = 11.84 EE64 pKa = 3.71 FARR67 pKa = 11.84 RR68 pKa = 11.84 LFLEE72 pKa = 4.34 YY73 pKa = 10.11 PPPTRR78 pKa = 11.84 KK79 pKa = 9.97 GRR81 pKa = 11.84 VLSLKK86 pKa = 8.45 EE87 pKa = 4.02 FKK89 pKa = 10.57 RR90 pKa = 11.84 ITSLGKK96 pKa = 10.09
Molecular weight: 11.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.261
IPC2_protein 9.648
IPC_protein 9.955
Toseland 10.789
ProMoST 10.379
Dawson 10.862
Bjellqvist 10.482
Wikipedia 10.994
Rodwell 11.301
Grimsley 10.877
Solomon 10.935
Lehninger 10.921
Nozaki 10.76
DTASelect 10.467
Thurlkill 10.76
EMBOSS 11.169
Sillero 10.774
Patrickios 11.038
IPC_peptide 10.95
IPC2_peptide 9.077
IPC2.peptide.svr19 8.73
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
57
0
57
10625
49
593
186.4
21.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.158 ± 0.324
1.12 ± 0.15
4.631 ± 0.319
6.936 ± 0.455
4.461 ± 0.239
5.449 ± 0.265
1.384 ± 0.181
8.687 ± 0.424
7.605 ± 0.552
9.289 ± 0.262
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.212 ± 0.163
5.12 ± 0.247
3.981 ± 0.301
3.313 ± 0.338
3.821 ± 0.332
6.456 ± 0.535
5.911 ± 0.435
8.132 ± 0.388
0.932 ± 0.114
5.393 ± 0.31
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here