Methanobrevibacter sp. AbM4

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanomada group; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanobrevibacter; unclassified Methanobrevibacter

Average proteome isoelectric point is 5.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1663 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9SJH5|R9SJH5_9EURY Acetolactate synthase OS=Methanobrevibacter sp. AbM4 OX=224719 GN=ilvB1 PE=3 SV=1
MM1 pKa = 8.2DD2 pKa = 6.17DD3 pKa = 4.17YY4 pKa = 11.89FNIDD8 pKa = 2.85EE9 pKa = 4.21WQNWVDD15 pKa = 4.03KK16 pKa = 10.57SFHH19 pKa = 5.9MFNGIIEE26 pKa = 4.42GGRR29 pKa = 11.84IGFLLEE35 pKa = 4.43GFDD38 pKa = 5.71DD39 pKa = 5.68SMILLTKK46 pKa = 10.2GVNNVDD52 pKa = 3.2VSLVNGLTDD61 pKa = 3.33VSLDD65 pKa = 3.59KK66 pKa = 11.17SHH68 pKa = 7.53ADD70 pKa = 3.23IIFKK74 pKa = 10.2VYY76 pKa = 10.32PSDD79 pKa = 4.74VEE81 pKa = 5.42DD82 pKa = 4.29LLEE85 pKa = 5.75DD86 pKa = 3.62YY87 pKa = 11.08DD88 pKa = 4.94FEE90 pKa = 4.85IFNEE94 pKa = 4.09LLEE97 pKa = 4.29KK98 pKa = 10.54EE99 pKa = 4.47YY100 pKa = 10.68IRR102 pKa = 11.84LYY104 pKa = 11.0NLLDD108 pKa = 3.95VEE110 pKa = 4.25EE111 pKa = 5.08LDD113 pKa = 3.93YY114 pKa = 11.67NGYY117 pKa = 8.51EE118 pKa = 4.13HH119 pKa = 7.07FLSRR123 pKa = 11.84LGFYY127 pKa = 10.58INGGPYY133 pKa = 10.23CGII136 pKa = 4.15

Molecular weight: 15.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9SKB8|R9SKB8_9EURY Flavoprotein HI0933 family OS=Methanobrevibacter sp. AbM4 OX=224719 GN=Abm4_0683 PE=4 SV=1
MM1 pKa = 7.33PANRR5 pKa = 11.84FRR7 pKa = 11.84SRR9 pKa = 11.84SYY11 pKa = 10.89KK12 pKa = 10.36RR13 pKa = 11.84MNKK16 pKa = 8.16RR17 pKa = 11.84TPGGEE22 pKa = 3.46NVLRR26 pKa = 11.84YY27 pKa = 9.41KK28 pKa = 10.6KK29 pKa = 10.06KK30 pKa = 9.74RR31 pKa = 11.84PSKK34 pKa = 10.14HH35 pKa = 4.87VCAEE39 pKa = 4.04CGAPLHH45 pKa = 6.27AVPNGRR51 pKa = 11.84PYY53 pKa = 10.62EE54 pKa = 4.02INKK57 pKa = 9.68LSKK60 pKa = 9.71SQKK63 pKa = 9.73RR64 pKa = 11.84PNRR67 pKa = 11.84PYY69 pKa = 11.0GGYY72 pKa = 10.19LCPKK76 pKa = 9.52CARR79 pKa = 11.84KK80 pKa = 9.42HH81 pKa = 5.5FKK83 pKa = 10.94NEE85 pKa = 3.2ARR87 pKa = 11.84KK88 pKa = 9.71

Molecular weight: 10.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1663

0

1663

501765

35

2615

301.7

33.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.507 ± 0.071

1.256 ± 0.03

6.867 ± 0.064

7.08 ± 0.091

4.145 ± 0.044

6.516 ± 0.072

1.614 ± 0.029

9.436 ± 0.073

7.956 ± 0.082

8.704 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.325 ± 0.039

6.724 ± 0.136

3.322 ± 0.042

2.24 ± 0.026

3.377 ± 0.045

6.515 ± 0.073

5.142 ± 0.065

6.497 ± 0.064

0.627 ± 0.015

4.15 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski