Amino acid dipepetide frequency for Methanobrevibacter sp. AbM4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.619AlaAla: 3.619 ± 0.118
0.873AlaCys: 0.873 ± 0.05
3.518AlaAsp: 3.518 ± 0.093
3.512AlaGlu: 3.512 ± 0.105
2.113AlaPhe: 2.113 ± 0.067
3.88AlaGly: 3.88 ± 0.102
0.943AlaHis: 0.943 ± 0.044
5.7AlaIle: 5.7 ± 0.144
4.098AlaLys: 4.098 ± 0.088
5.084AlaLeu: 5.084 ± 0.131
1.349AlaMet: 1.349 ± 0.061
2.991AlaAsn: 2.991 ± 0.102
1.606AlaPro: 1.606 ± 0.06
1.315AlaGln: 1.315 ± 0.055
1.985AlaArg: 1.985 ± 0.068
3.516AlaSer: 3.516 ± 0.084
2.681AlaThr: 2.681 ± 0.086
3.968AlaVal: 3.968 ± 0.096
0.249AlaTrp: 0.249 ± 0.022
2.073AlaTyr: 2.073 ± 0.058
0.0AlaXaa: 0.0 ± 0.0
Cys
0.672CysAla: 0.672 ± 0.044
0.201CysCys: 0.201 ± 0.02
0.807CysAsp: 0.807 ± 0.038
0.775CysGlu: 0.775 ± 0.044
0.43CysPhe: 0.43 ± 0.03
1.564CysGly: 1.564 ± 0.069
0.261CysHis: 0.261 ± 0.024
0.971CysIle: 0.971 ± 0.049
0.817CysLys: 0.817 ± 0.041
0.895CysLeu: 0.895 ± 0.044
0.265CysMet: 0.265 ± 0.025
0.696CysAsn: 0.696 ± 0.04
0.923CysPro: 0.923 ± 0.061
0.281CysGln: 0.281 ± 0.023
0.458CysArg: 0.458 ± 0.035
0.765CysSer: 0.765 ± 0.04
0.564CysThr: 0.564 ± 0.039
0.783CysVal: 0.783 ± 0.049
0.06CysTrp: 0.06 ± 0.011
0.373CysTyr: 0.373 ± 0.027
0.0CysXaa: 0.0 ± 0.0
Asp
3.35AspAla: 3.35 ± 0.091
0.807AspCys: 0.807 ± 0.039
4.865AspAsp: 4.865 ± 0.135
5.594AspGlu: 5.594 ± 0.127
3.203AspPhe: 3.203 ± 0.096
3.95AspGly: 3.95 ± 0.083
0.915AspHis: 0.915 ± 0.046
6.75AspIle: 6.75 ± 0.134
5.648AspLys: 5.648 ± 0.115
6.453AspLeu: 6.453 ± 0.122
1.887AspMet: 1.887 ± 0.062
4.701AspAsn: 4.701 ± 0.117
1.907AspPro: 1.907 ± 0.061
1.09AspGln: 1.09 ± 0.05
1.782AspArg: 1.782 ± 0.058
4.135AspSer: 4.135 ± 0.103
2.918AspThr: 2.918 ± 0.081
4.743AspVal: 4.743 ± 0.103
0.444AspTrp: 0.444 ± 0.033
3.528AspTyr: 3.528 ± 0.089
0.0AspXaa: 0.0 ± 0.0
Glu
3.85GluAla: 3.85 ± 0.104
0.67GluCys: 0.67 ± 0.044
5.742GluAsp: 5.742 ± 0.135
6.565GluGlu: 6.565 ± 0.157
2.97GluPhe: 2.97 ± 0.078
3.944GluGly: 3.944 ± 0.106
1.072GluHis: 1.072 ± 0.053
6.692GluIle: 6.692 ± 0.145
5.78GluLys: 5.78 ± 0.135
6.158GluLeu: 6.158 ± 0.132
1.826GluMet: 1.826 ± 0.066
5.405GluAsn: 5.405 ± 0.105
1.664GluPro: 1.664 ± 0.063
1.361GluGln: 1.361 ± 0.06
2.322GluArg: 2.322 ± 0.076
3.83GluSer: 3.83 ± 0.089
3.29GluThr: 3.29 ± 0.088
4.261GluVal: 4.261 ± 0.121
0.385GluTrp: 0.385 ± 0.028
3.009GluTyr: 3.009 ± 0.083
0.0GluXaa: 0.0 ± 0.0
Phe
2.17PheAla: 2.17 ± 0.069
0.514PheCys: 0.514 ± 0.036
2.972PheAsp: 2.972 ± 0.08
2.768PheGlu: 2.768 ± 0.085
1.551PhePhe: 1.551 ± 0.06
2.72PheGly: 2.72 ± 0.081
0.646PheHis: 0.646 ± 0.035
3.978PheIle: 3.978 ± 0.097
3.589PheLys: 3.589 ± 0.103
3.749PheLeu: 3.749 ± 0.111
0.985PheMet: 0.985 ± 0.045
3.049PheAsn: 3.049 ± 0.095
1.274PhePro: 1.274 ± 0.049
0.845PheGln: 0.845 ± 0.04
1.228PheArg: 1.228 ± 0.055
2.679PheSer: 2.679 ± 0.074
2.34PheThr: 2.34 ± 0.072
2.513PheVal: 2.513 ± 0.083
0.219PheTrp: 0.219 ± 0.021
1.662PheTyr: 1.662 ± 0.058
0.0PheXaa: 0.0 ± 0.0
Gly
4.086GlyAla: 4.086 ± 0.128
0.889GlyCys: 0.889 ± 0.05
3.817GlyAsp: 3.817 ± 0.091
3.976GlyGlu: 3.976 ± 0.096
3.121GlyPhe: 3.121 ± 0.089
4.317GlyGly: 4.317 ± 0.118
1.114GlyHis: 1.114 ± 0.051
6.395GlyIle: 6.395 ± 0.133
4.875GlyLys: 4.875 ± 0.106
5.638GlyLeu: 5.638 ± 0.142
1.539GlyMet: 1.539 ± 0.06
3.745GlyAsn: 3.745 ± 0.149
1.806GlyPro: 1.806 ± 0.06
1.562GlyGln: 1.562 ± 0.065
2.36GlyArg: 2.36 ± 0.075
4.381GlySer: 4.381 ± 0.111
3.623GlyThr: 3.623 ± 0.105
4.606GlyVal: 4.606 ± 0.109
0.474GlyTrp: 0.474 ± 0.033
2.834GlyTyr: 2.834 ± 0.08
0.0GlyXaa: 0.0 ± 0.0
His
0.815HisAla: 0.815 ± 0.049
0.313HisCys: 0.313 ± 0.029
1.002HisAsp: 1.002 ± 0.045
1.038HisGlu: 1.038 ± 0.05
0.596HisPhe: 0.596 ± 0.031
1.184HisGly: 1.184 ± 0.055
0.44HisHis: 0.44 ± 0.045
1.383HisIle: 1.383 ± 0.058
1.086HisLys: 1.086 ± 0.053
1.329HisLeu: 1.329 ± 0.057
0.425HisMet: 0.425 ± 0.031
0.943HisAsn: 0.943 ± 0.047
0.891HisPro: 0.891 ± 0.047
0.369HisGln: 0.369 ± 0.027
0.612HisArg: 0.612 ± 0.038
0.973HisSer: 0.973 ± 0.047
0.825HisThr: 0.825 ± 0.038
1.054HisVal: 1.054 ± 0.05
0.132HisTrp: 0.132 ± 0.016
0.731HisTyr: 0.731 ± 0.044
0.0HisXaa: 0.0 ± 0.0
Ile
5.668IleAla: 5.668 ± 0.138
1.114IleCys: 1.114 ± 0.054
6.336IleAsp: 6.336 ± 0.115
6.517IleGlu: 6.517 ± 0.123
3.823IlePhe: 3.823 ± 0.117
6.206IleGly: 6.206 ± 0.123
1.557IleHis: 1.557 ± 0.058
8.763IleIle: 8.763 ± 0.161
7.272IleLys: 7.272 ± 0.139
8.608IleLeu: 8.608 ± 0.153
1.917IleMet: 1.917 ± 0.059
6.248IleAsn: 6.248 ± 0.181
3.653IlePro: 3.653 ± 0.092
2.081IleGln: 2.081 ± 0.066
3.075IleArg: 3.075 ± 0.086
6.501IleSer: 6.501 ± 0.121
4.927IleThr: 4.927 ± 0.123
6.158IleVal: 6.158 ± 0.114
0.518IleTrp: 0.518 ± 0.031
3.422IleTyr: 3.422 ± 0.092
0.0IleXaa: 0.0 ± 0.0
Lys
4.231LysAla: 4.231 ± 0.098
0.961LysCys: 0.961 ± 0.051
5.979LysAsp: 5.979 ± 0.123
6.983LysGlu: 6.983 ± 0.144
2.808LysPhe: 2.808 ± 0.078
4.357LysGly: 4.357 ± 0.107
1.234LysHis: 1.234 ± 0.055
7.539LysIle: 7.539 ± 0.146
6.338LysLys: 6.338 ± 0.15
6.37LysLeu: 6.37 ± 0.129
1.648LysMet: 1.648 ± 0.05
5.293LysAsn: 5.293 ± 0.122
2.517LysPro: 2.517 ± 0.079
1.78LysGln: 1.78 ± 0.062
3.011LysArg: 3.011 ± 0.091
4.865LysSer: 4.865 ± 0.123
4.394LysThr: 4.394 ± 0.116
5.124LysVal: 5.124 ± 0.103
0.548LysTrp: 0.548 ± 0.039
3.577LysTyr: 3.577 ± 0.098
0.0LysXaa: 0.0 ± 0.0
Leu
4.959LeuAla: 4.959 ± 0.113
0.992LeuCys: 0.992 ± 0.052
6.292LeuAsp: 6.292 ± 0.127
5.917LeuGlu: 5.917 ± 0.119
3.697LeuPhe: 3.697 ± 0.118
5.728LeuGly: 5.728 ± 0.126
1.236LeuHis: 1.236 ± 0.051
8.323LeuIle: 8.323 ± 0.158
7.894LeuLys: 7.894 ± 0.142
6.786LeuLeu: 6.786 ± 0.162
2.091LeuMet: 2.091 ± 0.063
6.463LeuAsn: 6.463 ± 0.137
2.856LeuPro: 2.856 ± 0.074
1.706LeuGln: 1.706 ± 0.063
2.782LeuArg: 2.782 ± 0.09
6.172LeuSer: 6.172 ± 0.12
4.466LeuThr: 4.466 ± 0.103
5.17LeuVal: 5.17 ± 0.108
0.532LeuTrp: 0.532 ± 0.036
2.977LeuTyr: 2.977 ± 0.095
0.0LeuXaa: 0.0 ± 0.0
Met
1.614MetAla: 1.614 ± 0.066
0.295MetCys: 0.295 ± 0.025
1.879MetAsp: 1.879 ± 0.055
1.999MetGlu: 1.999 ± 0.077
0.909MetPhe: 0.909 ± 0.042
1.604MetGly: 1.604 ± 0.057
0.341MetHis: 0.341 ± 0.023
2.041MetIle: 2.041 ± 0.064
1.784MetLys: 1.784 ± 0.061
1.63MetLeu: 1.63 ± 0.07
0.6MetMet: 0.6 ± 0.034
1.272MetAsn: 1.272 ± 0.052
0.763MetPro: 0.763 ± 0.039
0.385MetGln: 0.385 ± 0.033
0.849MetArg: 0.849 ± 0.048
1.652MetSer: 1.652 ± 0.053
1.124MetThr: 1.124 ± 0.054
1.654MetVal: 1.654 ± 0.061
0.149MetTrp: 0.149 ± 0.016
0.708MetTyr: 0.708 ± 0.038
0.0MetXaa: 0.0 ± 0.0
Asn
2.786AsnAla: 2.786 ± 0.086
0.873AsnCys: 0.873 ± 0.053
3.836AsnAsp: 3.836 ± 0.098
3.97AsnGlu: 3.97 ± 0.098
2.597AsnPhe: 2.597 ± 0.082
4.096AsnGly: 4.096 ± 0.163
1.106AsnHis: 1.106 ± 0.044
6.804AsnIle: 6.804 ± 0.17
5.479AsnLys: 5.479 ± 0.136
5.871AsnLeu: 5.871 ± 0.142
1.638AsnMet: 1.638 ± 0.06
5.53AsnAsn: 5.53 ± 0.251
2.89AsnPro: 2.89 ± 0.072
1.911AsnGln: 1.911 ± 0.077
2.21AsnArg: 2.21 ± 0.076
4.394AsnSer: 4.394 ± 0.177
3.669AsnThr: 3.669 ± 0.152
4.153AsnVal: 4.153 ± 0.136
0.466AsnTrp: 0.466 ± 0.033
2.96AsnTyr: 2.96 ± 0.108
0.0AsnXaa: 0.0 ± 0.0
Pro
1.732ProAla: 1.732 ± 0.063
0.367ProCys: 0.367 ± 0.025
2.186ProAsp: 2.186 ± 0.076
2.896ProGlu: 2.896 ± 0.09
1.461ProPhe: 1.461 ± 0.05
2.198ProGly: 2.198 ± 0.064
0.684ProHis: 0.684 ± 0.041
3.093ProIle: 3.093 ± 0.079
2.463ProLys: 2.463 ± 0.085
2.824ProLeu: 2.824 ± 0.082
0.733ProMet: 0.733 ± 0.04
1.955ProAsn: 1.955 ± 0.052
0.813ProPro: 0.813 ± 0.042
0.859ProGln: 0.859 ± 0.041
1.086ProArg: 1.086 ± 0.056
2.095ProSer: 2.095 ± 0.064
1.714ProThr: 1.714 ± 0.061
2.386ProVal: 2.386 ± 0.071
0.203ProTrp: 0.203 ± 0.021
1.469ProTyr: 1.469 ± 0.049
0.0ProXaa: 0.0 ± 0.0
Gln
1.381GlnAla: 1.381 ± 0.056
0.269GlnCys: 0.269 ± 0.023
1.291GlnAsp: 1.291 ± 0.055
1.63GlnGlu: 1.63 ± 0.06
0.901GlnPhe: 0.901 ± 0.038
1.341GlnGly: 1.341 ± 0.055
0.359GlnHis: 0.359 ± 0.025
2.081GlnIle: 2.081 ± 0.068
1.778GlnLys: 1.778 ± 0.058
2.015GlnLeu: 2.015 ± 0.065
0.66GlnMet: 0.66 ± 0.038
1.277GlnAsn: 1.277 ± 0.058
0.548GlnPro: 0.548 ± 0.032
0.62GlnGln: 0.62 ± 0.051
0.845GlnArg: 0.845 ± 0.039
1.515GlnSer: 1.515 ± 0.058
1.142GlnThr: 1.142 ± 0.049
1.491GlnVal: 1.491 ± 0.056
0.161GlnTrp: 0.161 ± 0.019
1.098GlnTyr: 1.098 ± 0.049
0.0GlnXaa: 0.0 ± 0.0
Arg
1.843ArgAla: 1.843 ± 0.069
0.444ArgCys: 0.444 ± 0.034
2.21ArgAsp: 2.21 ± 0.08
2.647ArgGlu: 2.647 ± 0.087
1.527ArgPhe: 1.527 ± 0.059
2.091ArgGly: 2.091 ± 0.076
0.488ArgHis: 0.488 ± 0.033
3.167ArgIle: 3.167 ± 0.08
3.011ArgLys: 3.011 ± 0.097
2.94ArgLeu: 2.94 ± 0.083
0.781ArgMet: 0.781 ± 0.042
2.023ArgAsn: 2.023 ± 0.07
1.078ArgPro: 1.078 ± 0.055
0.875ArgGln: 0.875 ± 0.042
1.541ArgArg: 1.541 ± 0.075
1.857ArgSer: 1.857 ± 0.066
1.612ArgThr: 1.612 ± 0.057
2.003ArgVal: 2.003 ± 0.069
0.233ArgTrp: 0.233 ± 0.024
1.395ArgTyr: 1.395 ± 0.059
0.0ArgXaa: 0.0 ± 0.0
Ser
3.215SerAla: 3.215 ± 0.103
0.662SerCys: 0.662 ± 0.042
4.351SerAsp: 4.351 ± 0.107
3.956SerGlu: 3.956 ± 0.092
2.904SerPhe: 2.904 ± 0.099
4.422SerGly: 4.422 ± 0.113
1.16SerHis: 1.16 ± 0.053
5.748SerIle: 5.748 ± 0.118
5.453SerLys: 5.453 ± 0.126
5.875SerLeu: 5.875 ± 0.125
1.381SerMet: 1.381 ± 0.044
4.636SerAsn: 4.636 ± 0.174
2.053SerPro: 2.053 ± 0.067
1.752SerGln: 1.752 ± 0.058
2.246SerArg: 2.246 ± 0.066
4.945SerSer: 4.945 ± 0.159
3.47SerThr: 3.47 ± 0.108
3.781SerVal: 3.781 ± 0.094
0.413SerTrp: 0.413 ± 0.031
2.734SerTyr: 2.734 ± 0.097
0.0SerXaa: 0.0 ± 0.0
Thr
2.97ThrAla: 2.97 ± 0.084
0.672ThrCys: 0.672 ± 0.04
3.464ThrAsp: 3.464 ± 0.091
2.84ThrGlu: 2.84 ± 0.08
2.093ThrPhe: 2.093 ± 0.071
3.902ThrGly: 3.902 ± 0.09
0.845ThrHis: 0.845 ± 0.045
5.072ThrIle: 5.072 ± 0.156
3.382ThrLys: 3.382 ± 0.091
4.512ThrLeu: 4.512 ± 0.104
1.046ThrMet: 1.046 ± 0.04
3.177ThrAsn: 3.177 ± 0.12
2.009ThrPro: 2.009 ± 0.062
1.094ThrGln: 1.094 ± 0.05
1.73ThrArg: 1.73 ± 0.063
3.374ThrSer: 3.374 ± 0.095
2.91ThrThr: 2.91 ± 0.108
3.964ThrVal: 3.964 ± 0.139
0.291ThrTrp: 0.291 ± 0.028
2.073ThrTyr: 2.073 ± 0.086
0.0ThrXaa: 0.0 ± 0.0
Val
3.838ValAla: 3.838 ± 0.097
0.915ValCys: 0.915 ± 0.044
4.638ValAsp: 4.638 ± 0.101
4.307ValGlu: 4.307 ± 0.1
2.689ValPhe: 2.689 ± 0.076
4.283ValGly: 4.283 ± 0.113
0.985ValHis: 0.985 ± 0.049
5.867ValIle: 5.867 ± 0.111
5.178ValLys: 5.178 ± 0.097
5.869ValLeu: 5.869 ± 0.119
1.355ValMet: 1.355 ± 0.055
4.287ValAsn: 4.287 ± 0.149
2.264ValPro: 2.264 ± 0.067
1.339ValGln: 1.339 ± 0.052
2.017ValArg: 2.017 ± 0.062
4.528ValSer: 4.528 ± 0.103
3.251ValThr: 3.251 ± 0.106
4.377ValVal: 4.377 ± 0.116
0.397ValTrp: 0.397 ± 0.031
2.589ValTyr: 2.589 ± 0.077
0.0ValXaa: 0.0 ± 0.0
Trp
0.349TrpAla: 0.349 ± 0.027
0.058TrpCys: 0.058 ± 0.011
0.45TrpAsp: 0.45 ± 0.029
0.359TrpGlu: 0.359 ± 0.027
0.257TrpPhe: 0.257 ± 0.024
0.44TrpGly: 0.44 ± 0.034
0.082TrpHis: 0.082 ± 0.013
0.584TrpIle: 0.584 ± 0.034
0.464TrpLys: 0.464 ± 0.032
0.58TrpLeu: 0.58 ± 0.035
0.185TrpMet: 0.185 ± 0.016
0.432TrpAsn: 0.432 ± 0.03
0.167TrpPro: 0.167 ± 0.018
0.185TrpGln: 0.185 ± 0.022
0.261TrpArg: 0.261 ± 0.023
0.339TrpSer: 0.339 ± 0.028
0.311TrpThr: 0.311 ± 0.025
0.391TrpVal: 0.391 ± 0.029
0.1TrpTrp: 0.1 ± 0.015
0.279TrpTyr: 0.279 ± 0.025
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.923TyrAla: 1.923 ± 0.066
0.674TyrCys: 0.674 ± 0.036
3.039TyrAsp: 3.039 ± 0.085
2.547TyrGlu: 2.547 ± 0.085
1.804TyrPhe: 1.804 ± 0.066
2.928TyrGly: 2.928 ± 0.093
0.676TyrHis: 0.676 ± 0.036
3.412TyrIle: 3.412 ± 0.08
3.169TyrLys: 3.169 ± 0.091
3.852TyrLeu: 3.852 ± 0.095
0.939TyrMet: 0.939 ± 0.041
3.117TyrAsn: 3.117 ± 0.103
1.535TyrPro: 1.535 ± 0.054
0.992TyrGln: 0.992 ± 0.043
1.367TyrArg: 1.367 ± 0.056
2.639TyrSer: 2.639 ± 0.075
2.188TyrThr: 2.188 ± 0.074
2.392TyrVal: 2.392 ± 0.073
0.299TyrTrp: 0.299 ± 0.027
2.005TyrTyr: 2.005 ± 0.099
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1663 proteins (501765 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski