Turkey associated porprismacovirus 1
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W0GEQ0|W0GEQ0_9VIRU Capsid protein OS=Turkey associated porprismacovirus 1 OX=2170130 PE=4 SV=1
MM1 pKa = 6.48 VAVRR5 pKa = 11.84 VSEE8 pKa = 4.31 TYY10 pKa = 10.76 DD11 pKa = 3.27 LSTKK15 pKa = 9.04 IGKK18 pKa = 8.47 MGLVGIHH25 pKa = 6.31 TPTGDD30 pKa = 4.17 LISRR34 pKa = 11.84 MWPGLILQHH43 pKa = 6.04 EE44 pKa = 4.62 KK45 pKa = 10.79 FRR47 pKa = 11.84 FVKK50 pKa = 10.39 CDD52 pKa = 2.95 VAMACASMLPADD64 pKa = 4.26 PLQIGVEE71 pKa = 4.06 AGAIAPQDD79 pKa = 3.54 MFNPILYY86 pKa = 9.62 KK87 pKa = 10.55 AVSNEE92 pKa = 4.08 SMNQFQAWLNIVNQTAGLTGAFNQGSVVDD121 pKa = 4.11 VNDD124 pKa = 4.13 PNFVGANGTHH134 pKa = 6.93 EE135 pKa = 4.16 YY136 pKa = 10.78 DD137 pKa = 3.44 QFSMYY142 pKa = 10.32 YY143 pKa = 10.72 ALLADD148 pKa = 3.43 SDD150 pKa = 4.11 GWRR153 pKa = 11.84 KK154 pKa = 10.33 SMPQAGLEE162 pKa = 4.0 MRR164 pKa = 11.84 GLYY167 pKa = 9.85 PLVFQVVSQFGSGPEE182 pKa = 3.96 NSAGDD187 pKa = 3.93 ADD189 pKa = 4.66 SNISVPGLSGEE200 pKa = 4.37 AADD203 pKa = 4.88 ASPTSRR209 pKa = 11.84 FGTVSSIQNRR219 pKa = 11.84 TMRR222 pKa = 11.84 GPSMRR227 pKa = 11.84 MPAIDD232 pKa = 3.04 TTFFVNSTSTDD243 pKa = 3.29 SALLPGGINVDD254 pKa = 3.38 TSLDD258 pKa = 3.94 KK259 pKa = 11.4 VPPAYY264 pKa = 10.27 VGLIILPPARR274 pKa = 11.84 LNQLYY279 pKa = 10.21 YY280 pKa = 10.31 RR281 pKa = 11.84 LKK283 pKa = 8.78 VTWTIEE289 pKa = 4.03 FTGLRR294 pKa = 11.84 SAVDD298 pKa = 2.97 ISNWFNLQNIGQLAYY313 pKa = 10.38 GSDD316 pKa = 4.08 YY317 pKa = 10.93 EE318 pKa = 4.37 SQSAKK323 pKa = 9.97 MSAKK327 pKa = 9.7 TGMVDD332 pKa = 3.29 TTGSSITKK340 pKa = 9.95 IMEE343 pKa = 4.19 GSS345 pKa = 3.18
Molecular weight: 37.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.81
IPC2_protein 4.863
IPC_protein 4.787
Toseland 4.647
ProMoST 4.927
Dawson 4.774
Bjellqvist 4.914
Wikipedia 4.673
Rodwell 4.647
Grimsley 4.558
Solomon 4.774
Lehninger 4.724
Nozaki 4.889
DTASelect 5.105
Thurlkill 4.66
EMBOSS 4.698
Sillero 4.927
Patrickios 3.923
IPC_peptide 4.774
IPC2_peptide 4.914
IPC2.peptide.svr19 4.925
Protein with the highest isoelectric point:
>tr|W0GEQ0|W0GEQ0_9VIRU Capsid protein OS=Turkey associated porprismacovirus 1 OX=2170130 PE=4 SV=1
MM1 pKa = 7.58 KK2 pKa = 9.88 KK3 pKa = 10.66 YY4 pKa = 10.74 IMTVPRR10 pKa = 11.84 SVPKK14 pKa = 10.24 KK15 pKa = 9.7 ALKK18 pKa = 10.51 IMIDD22 pKa = 3.62 VNDD25 pKa = 3.89 CKK27 pKa = 10.84 KK28 pKa = 10.09 WIIGKK33 pKa = 8.89 EE34 pKa = 3.81 RR35 pKa = 11.84 GKK37 pKa = 10.39 NGYY40 pKa = 7.35 EE41 pKa = 3.32 HH42 pKa = 5.58 WQIRR46 pKa = 11.84 IEE48 pKa = 4.1 TSNDD52 pKa = 3.12 EE53 pKa = 3.95 FFKK56 pKa = 10.0 WCKK59 pKa = 9.35 HH60 pKa = 6.21 HH61 pKa = 7.13 IPAAHH66 pKa = 6.86 IEE68 pKa = 4.03 EE69 pKa = 4.45 AQQGVDD75 pKa = 2.8 EE76 pKa = 4.66 CLYY79 pKa = 10.31 EE80 pKa = 4.45 RR81 pKa = 11.84 KK82 pKa = 9.86 EE83 pKa = 4.17 GQFWTSSDD91 pKa = 3.32 RR92 pKa = 11.84 VEE94 pKa = 4.28 TLHH97 pKa = 6.68 QRR99 pKa = 11.84 FGTLRR104 pKa = 11.84 PRR106 pKa = 11.84 QEE108 pKa = 3.89 RR109 pKa = 11.84 ALLALQSTNDD119 pKa = 3.5 RR120 pKa = 11.84 EE121 pKa = 4.33 VMVWYY126 pKa = 9.52 DD127 pKa = 3.23 ANGNVGKK134 pKa = 10.23 SWFCGALWEE143 pKa = 4.8 RR144 pKa = 11.84 GLAYY148 pKa = 7.93 VTPPTVDD155 pKa = 3.07 TVKK158 pKa = 11.13 GLIQWVASCYY168 pKa = 9.46 MDD170 pKa = 4.24 GGWRR174 pKa = 11.84 PYY176 pKa = 10.86 VIIDD180 pKa = 4.59 IPRR183 pKa = 11.84 SWKK186 pKa = 9.98 WSDD189 pKa = 3.42 QLYY192 pKa = 10.52 CAIEE196 pKa = 4.31 SIKK199 pKa = 11.03 DD200 pKa = 3.19 GLIYY204 pKa = 9.12 DD205 pKa = 4.11 TRR207 pKa = 11.84 YY208 pKa = 7.89 HH209 pKa = 6.86 ARR211 pKa = 11.84 MINIRR216 pKa = 11.84 GVKK219 pKa = 10.04 VLVLTNTLPKK229 pKa = 9.94 LDD231 pKa = 3.78 KK232 pKa = 10.63 LSRR235 pKa = 11.84 DD236 pKa = 3.02 RR237 pKa = 11.84 WCIFEE242 pKa = 3.94 FF243 pKa = 4.66
Molecular weight: 28.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.06
IPC2_protein 8.141
IPC_protein 8.039
Toseland 8.053
ProMoST 8.551
Dawson 8.799
Bjellqvist 8.99
Wikipedia 8.799
Rodwell 8.873
Grimsley 7.936
Solomon 8.931
Lehninger 8.931
Nozaki 9.165
DTASelect 8.77
Thurlkill 8.858
EMBOSS 8.99
Sillero 9.121
Patrickios 4.52
IPC_peptide 8.931
IPC2_peptide 7.849
IPC2.peptide.svr19 7.892
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
588
243
345
294.0
32.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.973 ± 1.164
1.531 ± 0.773
5.952 ± 0.126
4.422 ± 1.002
3.571 ± 0.395
7.823 ± 0.944
1.531 ± 0.537
6.463 ± 0.776
5.102 ± 1.555
7.483 ± 0.279
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.741 ± 0.728
4.422 ± 0.646
4.762 ± 0.606
4.252 ± 0.314
5.102 ± 1.319
7.653 ± 1.789
5.782 ± 0.483
6.973 ± 0.222
2.891 ± 1.171
3.571 ± 0.311
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here