Candidatus Mycoplasma girerdii
Average proteome isoelectric point is 7.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 572 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A097SSX4|A0A097SSX4_9MOLU Glycerol-3-phosphate acyltransferase OS=Candidatus Mycoplasma girerdii OX=1318617 GN=plsY PE=3 SV=1
MM1 pKa = 7.44 SRR3 pKa = 11.84 LLIDD7 pKa = 3.4 VAYY10 pKa = 10.35 DD11 pKa = 3.19 IAKK14 pKa = 10.46 QNFKK18 pKa = 10.75 KK19 pKa = 10.46 EE20 pKa = 4.06 QFSFEE25 pKa = 4.46 SLWDD29 pKa = 3.71 LVTKK33 pKa = 10.47 KK34 pKa = 9.42 MRR36 pKa = 11.84 YY37 pKa = 7.37 SQEE40 pKa = 4.0 QIEE43 pKa = 4.42 SEE45 pKa = 3.7 IGEE48 pKa = 4.47 FYY50 pKa = 10.94 SDD52 pKa = 2.92 IMQDD56 pKa = 2.86 SRR58 pKa = 11.84 FIFCGRR64 pKa = 11.84 NHH66 pKa = 6.24 WRR68 pKa = 11.84 LSEE71 pKa = 3.91 YY72 pKa = 11.0 LKK74 pKa = 10.88 LDD76 pKa = 3.88 EE77 pKa = 4.48 KK78 pKa = 11.51 HH79 pKa = 6.41 NMQNMLYY86 pKa = 10.3 DD87 pKa = 4.45 LNQEE91 pKa = 3.99 ICEE94 pKa = 4.04 EE95 pKa = 4.9 GYY97 pKa = 11.21 DD98 pKa = 4.08 KK99 pKa = 11.63 VNEE102 pKa = 4.19 KK103 pKa = 8.87 PTLNDD108 pKa = 3.57 DD109 pKa = 3.84 EE110 pKa = 5.39 EE111 pKa = 4.51 EE112 pKa = 4.44 LDD114 pKa = 3.83 EE115 pKa = 5.29 QDD117 pKa = 4.46 IEE119 pKa = 4.41 INEE122 pKa = 3.98 EE123 pKa = 3.66 NATEE127 pKa = 4.27 FEE129 pKa = 4.49 INDD132 pKa = 3.76 EE133 pKa = 4.23 EE134 pKa = 4.31 EE135 pKa = 3.84 ATYY138 pKa = 11.26 EE139 pKa = 4.38 NVGKK143 pKa = 10.14 LLKK146 pKa = 10.57 SSNDD150 pKa = 3.12 KK151 pKa = 9.81 TVDD154 pKa = 3.34 GDD156 pKa = 3.68 EE157 pKa = 4.44 EE158 pKa = 4.18 EE159 pKa = 4.26
Molecular weight: 19.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.286
IPC2_protein 4.139
IPC_protein 4.088
Toseland 3.923
ProMoST 4.177
Dawson 4.037
Bjellqvist 4.215
Wikipedia 3.897
Rodwell 3.923
Grimsley 3.834
Solomon 4.037
Lehninger 3.986
Nozaki 4.139
DTASelect 4.279
Thurlkill 3.923
EMBOSS 3.923
Sillero 4.202
Patrickios 3.592
IPC_peptide 4.037
IPC2_peptide 4.19
IPC2.peptide.svr19 4.109
Protein with the highest isoelectric point:
>tr|A0A097SSC7|A0A097SSC7_9MOLU Putative metal-dependent hydrolase OS=Candidatus Mycoplasma girerdii OX=1318617 GN=MGM1_1110 PE=4 SV=1
MM1 pKa = 7.58 AKK3 pKa = 9.99 KK4 pKa = 10.56 SLIAKK9 pKa = 7.45 QKK11 pKa = 10.21 RR12 pKa = 11.84 VPKK15 pKa = 9.89 YY16 pKa = 8.12 KK17 pKa = 8.93 TRR19 pKa = 11.84 GYY21 pKa = 7.88 TRR23 pKa = 11.84 CVRR26 pKa = 11.84 CGRR29 pKa = 11.84 PHH31 pKa = 6.07 AVNRR35 pKa = 11.84 KK36 pKa = 8.92 FGLCRR41 pKa = 11.84 ICLRR45 pKa = 11.84 EE46 pKa = 3.88 LAYY49 pKa = 10.58 AGAIPGLKK57 pKa = 9.72 KK58 pKa = 10.88 SSWW61 pKa = 3.3
Molecular weight: 6.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.307
IPC2_protein 10.043
IPC_protein 10.833
Toseland 11.067
ProMoST 10.789
Dawson 11.14
Bjellqvist 10.847
Wikipedia 11.345
Rodwell 11.462
Grimsley 11.169
Solomon 11.286
Lehninger 11.257
Nozaki 11.067
DTASelect 10.847
Thurlkill 11.052
EMBOSS 11.477
Sillero 11.067
Patrickios 11.199
IPC_peptide 11.301
IPC2_peptide 10.043
IPC2.peptide.svr19 8.233
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
572
0
572
190691
34
1630
333.4
37.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.434 ± 0.089
1.239 ± 0.038
5.155 ± 0.073
5.294 ± 0.107
4.975 ± 0.08
5.129 ± 0.087
1.906 ± 0.046
9.941 ± 0.098
9.233 ± 0.136
9.196 ± 0.089
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.995 ± 0.052
8.165 ± 0.122
2.859 ± 0.045
3.303 ± 0.054
2.953 ± 0.065
6.498 ± 0.14
5.635 ± 0.099
5.786 ± 0.064
0.994 ± 0.038
4.31 ± 0.071
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here