Kitasatospora griseola (Streptomyces griseolosporeus)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales;

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5875 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D0PYI2|A0A0D0PYI2_KITGR Membrane protein OS=Kitasatospora griseola OX=2064 GN=TR51_15925 PE=4 SV=1
MM1 pKa = 7.74AGTGEE6 pKa = 4.21ALEE9 pKa = 4.12VWIDD13 pKa = 3.41QDD15 pKa = 3.99LCTGDD20 pKa = 5.01GICAQYY26 pKa = 10.9APEE29 pKa = 4.23VFEE32 pKa = 5.9LDD34 pKa = 3.05IDD36 pKa = 3.65GLAYY40 pKa = 10.69VKK42 pKa = 11.17GEE44 pKa = 4.07DD45 pKa = 4.75DD46 pKa = 4.81EE47 pKa = 6.03LRR49 pKa = 11.84QQPGEE54 pKa = 4.23TVPVPLTLLKK64 pKa = 10.93DD65 pKa = 3.8VVDD68 pKa = 4.34SVKK71 pKa = 10.66DD72 pKa = 3.68CPGDD76 pKa = 4.09CIHH79 pKa = 6.54VRR81 pKa = 11.84RR82 pKa = 11.84ASDD85 pKa = 3.4GVEE88 pKa = 4.26VYY90 pKa = 10.92GPDD93 pKa = 3.44ADD95 pKa = 3.69

Molecular weight: 10.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D0PWT8|A0A0D0PWT8_KITGR Protein phosphatase OS=Kitasatospora griseola OX=2064 GN=TR51_29940 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.3GRR40 pKa = 11.84SALSAA45 pKa = 3.8

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5875

0

5875

1865248

29

7590

317.5

33.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.265 ± 0.054

0.794 ± 0.009

5.918 ± 0.025

5.528 ± 0.035

2.732 ± 0.019

9.374 ± 0.031

2.351 ± 0.019

3.016 ± 0.024

1.928 ± 0.025

10.917 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.63 ± 0.014

1.803 ± 0.022

6.156 ± 0.031

2.907 ± 0.02

7.973 ± 0.042

4.787 ± 0.026

6.011 ± 0.032

8.396 ± 0.033

1.525 ± 0.015

1.991 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski