Rhizobium sp. Root1212

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; unclassified Rhizobium

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4824 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q6T3D2|A0A0Q6T3D2_9RHIZ Phosphoglycerate mutase OS=Rhizobium sp. Root1212 OX=1736429 GN=ASC86_05685 PE=4 SV=1
MM1 pKa = 7.49NIKK4 pKa = 10.39SLLLGSAAALAAVSGAHH21 pKa = 6.45AADD24 pKa = 5.02AIVAAEE30 pKa = 4.47PEE32 pKa = 3.93PMEE35 pKa = 4.1YY36 pKa = 10.8VRR38 pKa = 11.84VCDD41 pKa = 4.42AFGTGYY47 pKa = 10.47FYY49 pKa = 10.57IPGTEE54 pKa = 3.93TCLSISGYY62 pKa = 10.28VRR64 pKa = 11.84TQIEE68 pKa = 4.65YY69 pKa = 10.2NDD71 pKa = 3.69SRR73 pKa = 11.84ADD75 pKa = 3.76DD76 pKa = 5.17DD77 pKa = 4.78DD78 pKa = 3.52WGTYY82 pKa = 6.98TRR84 pKa = 11.84AYY86 pKa = 9.6LTFAAKK92 pKa = 10.44NDD94 pKa = 4.01TEE96 pKa = 4.51FGTLSSYY103 pKa = 11.36INLQADD109 pKa = 3.68SGHH112 pKa = 5.71TWDD115 pKa = 4.31VGSGGDD121 pKa = 4.56VILDD125 pKa = 3.79GAWIEE130 pKa = 3.93IAGFKK135 pKa = 9.95IGYY138 pKa = 8.69FYY140 pKa = 11.36NWWDD144 pKa = 3.64DD145 pKa = 3.6FGLAGEE151 pKa = 4.27TDD153 pKa = 3.44EE154 pKa = 5.27TGGNLFNSIQYY165 pKa = 9.47TYY167 pKa = 11.0DD168 pKa = 3.29AGSFQVGASVEE179 pKa = 4.45DD180 pKa = 3.94LDD182 pKa = 6.12ADD184 pKa = 3.82VGSVYY189 pKa = 10.85SADD192 pKa = 3.24QGFGHH197 pKa = 6.74NDD199 pKa = 3.32DD200 pKa = 4.16VGISGLVRR208 pKa = 11.84GTFGGVDD215 pKa = 3.37ALLIASYY222 pKa = 11.26DD223 pKa = 3.63FDD225 pKa = 4.1VEE227 pKa = 4.0EE228 pKa = 4.42AAFKK232 pKa = 10.98ARR234 pKa = 11.84LTAAVGPGTFGIAGIYY250 pKa = 8.57ATDD253 pKa = 3.97PNAYY257 pKa = 8.86WSDD260 pKa = 4.04SEE262 pKa = 4.23WSVVASYY269 pKa = 10.1EE270 pKa = 4.54LKK272 pKa = 10.3ATDD275 pKa = 4.35KK276 pKa = 10.55FTITPAAQYY285 pKa = 9.91FGSLRR290 pKa = 11.84DD291 pKa = 3.76GTNGFGDD298 pKa = 3.72DD299 pKa = 4.31DD300 pKa = 3.63AWKK303 pKa = 10.42VGLTAGYY310 pKa = 10.22QITDD314 pKa = 3.59GLRR317 pKa = 11.84TLATINYY324 pKa = 9.04KK325 pKa = 10.66KK326 pKa = 10.0IDD328 pKa = 3.83TDD330 pKa = 4.14ADD332 pKa = 3.48IDD334 pKa = 4.22DD335 pKa = 4.91DD336 pKa = 4.93SISGFVRR343 pKa = 11.84LQRR346 pKa = 11.84DD347 pKa = 3.62FF348 pKa = 4.22

Molecular weight: 37.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q6T555|A0A0Q6T555_9RHIZ Uncharacterized protein OS=Rhizobium sp. Root1212 OX=1736429 GN=ASC86_06310 PE=4 SV=1
MM1 pKa = 7.62TKK3 pKa = 9.11RR4 pKa = 11.84TYY6 pKa = 10.36QPSKK10 pKa = 9.73LVRR13 pKa = 11.84KK14 pKa = 9.15RR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 4.64GFRR20 pKa = 11.84ARR22 pKa = 11.84IATKK26 pKa = 9.98GGRR29 pKa = 11.84KK30 pKa = 9.73VIVARR35 pKa = 11.84RR36 pKa = 11.84ARR38 pKa = 11.84GRR40 pKa = 11.84KK41 pKa = 9.03RR42 pKa = 11.84LSAA45 pKa = 4.03

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4824

0

4824

1524751

28

2877

316.1

34.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.913 ± 0.052

0.796 ± 0.01

5.801 ± 0.026

5.817 ± 0.031

3.943 ± 0.024

8.43 ± 0.036

1.976 ± 0.015

5.677 ± 0.026

3.794 ± 0.026

9.951 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.573 ± 0.016

2.86 ± 0.023

4.804 ± 0.028

3.021 ± 0.02

6.549 ± 0.037

5.734 ± 0.026

5.458 ± 0.029

7.323 ± 0.028

1.239 ± 0.014

2.339 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski