Methanobrevibacter filiformis

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanomada group; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanobrevibacter

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1920 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A166FFZ0|A0A166FFZ0_9EURY Uncharacterized protein OS=Methanobrevibacter filiformis OX=55758 GN=MBFIL_00230 PE=4 SV=1
MM1 pKa = 8.21AEE3 pKa = 3.81EE4 pKa = 5.8DD5 pKa = 3.82YY6 pKa = 11.6DD7 pKa = 4.34FDD9 pKa = 5.36EE10 pKa = 4.57EE11 pKa = 4.8TEE13 pKa = 4.56DD14 pKa = 3.59SLKK17 pKa = 10.38MFTGSVIDD25 pKa = 3.38IQYY28 pKa = 10.97LVDD31 pKa = 5.75NMFLDD36 pKa = 4.11YY37 pKa = 10.59KK38 pKa = 11.15SKK40 pKa = 11.07FEE42 pKa = 4.07NQEE45 pKa = 3.96SPISYY50 pKa = 10.06LDD52 pKa = 3.35EE53 pKa = 6.28GGDD56 pKa = 3.52ARR58 pKa = 11.84NLFEE62 pKa = 5.08TISLPLFEE70 pKa = 5.35IFWLINEE77 pKa = 4.44SVLMKK82 pKa = 9.06YY83 pKa = 9.89IPYY86 pKa = 10.52ASGDD90 pKa = 3.57FLDD93 pKa = 5.06ILGHH97 pKa = 5.78NNPRR101 pKa = 11.84NPGKK105 pKa = 10.38NSTGQLQFSLPHH117 pKa = 6.96DD118 pKa = 3.65VLKK121 pKa = 10.84DD122 pKa = 3.53YY123 pKa = 10.98DD124 pKa = 3.84ITIPAYY130 pKa = 10.07SVYY133 pKa = 9.82LTEE136 pKa = 6.15DD137 pKa = 3.64DD138 pKa = 5.49LGLEE142 pKa = 4.27YY143 pKa = 9.82EE144 pKa = 4.64TIEE147 pKa = 4.64EE148 pKa = 4.8AILPAGEE155 pKa = 4.05NSILVPARR163 pKa = 11.84SVFGGSEE170 pKa = 4.03YY171 pKa = 11.01NIDD174 pKa = 3.91SNLVTIFEE182 pKa = 4.19EE183 pKa = 4.93SIDD186 pKa = 3.98DD187 pKa = 4.26LLVTNPVPFIGGTDD201 pKa = 3.76DD202 pKa = 5.64EE203 pKa = 5.78DD204 pKa = 4.71DD205 pKa = 3.62EE206 pKa = 5.11YY207 pKa = 11.93YY208 pKa = 10.38RR209 pKa = 11.84ARR211 pKa = 11.84LLYY214 pKa = 9.43EE215 pKa = 3.78QSEE218 pKa = 4.46HH219 pKa = 7.55DD220 pKa = 3.82FGSVGWYY227 pKa = 9.92KK228 pKa = 10.79RR229 pKa = 11.84IAVKK233 pKa = 10.39IPGVHH238 pKa = 6.29DD239 pKa = 3.69VKK241 pKa = 11.24VINCLKK247 pKa = 10.23GDD249 pKa = 3.73YY250 pKa = 10.14TIGIIVNPPTAEE262 pKa = 3.38IVQNVTNFFNTADD275 pKa = 3.72TTPAGINAYY284 pKa = 9.38IYY286 pKa = 10.38GAEE289 pKa = 4.19TFPVDD294 pKa = 5.5IIIQDD299 pKa = 3.76LVFTNEE305 pKa = 3.69VNPADD310 pKa = 3.73VVEE313 pKa = 5.21EE314 pKa = 4.01IQNQINKK321 pKa = 9.33YY322 pKa = 9.77FNNLNISEE330 pKa = 4.49TIYY333 pKa = 10.83RR334 pKa = 11.84NSILSILSNINGLIDD349 pKa = 3.56YY350 pKa = 7.46TLISPSVNIDD360 pKa = 3.22AEE362 pKa = 4.44EE363 pKa = 4.1NQVLIQGEE371 pKa = 4.45TIINN375 pKa = 3.56

Molecular weight: 42.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A166ESV9|A0A166ESV9_9EURY Modification methylase HaeIII OS=Methanobrevibacter filiformis OX=55758 GN=haeIIIM PE=3 SV=1
MM1 pKa = 6.68VRR3 pKa = 11.84AYY5 pKa = 10.52SRR7 pKa = 11.84RR8 pKa = 11.84EE9 pKa = 3.95YY10 pKa = 10.02IRR12 pKa = 11.84KK13 pKa = 9.13IPGSRR18 pKa = 11.84IVQYY22 pKa = 11.77DD23 pKa = 3.51MGNLSQRR30 pKa = 11.84FPVAVSLAVKK40 pKa = 8.41TPARR44 pKa = 11.84ITHH47 pKa = 6.32NALEE51 pKa = 4.49AGRR54 pKa = 11.84VASNRR59 pKa = 11.84FMQRR63 pKa = 11.84KK64 pKa = 8.84AGRR67 pKa = 11.84LGYY70 pKa = 9.36HH71 pKa = 6.23LKK73 pKa = 10.25IRR75 pKa = 11.84VYY77 pKa = 7.96PHH79 pKa = 6.37NIIRR83 pKa = 11.84EE84 pKa = 4.11NPMATGAGADD94 pKa = 3.36RR95 pKa = 11.84VQDD98 pKa = 3.52GMRR101 pKa = 11.84KK102 pKa = 9.49AFGKK106 pKa = 10.01SVSSEE111 pKa = 3.7AVVKK115 pKa = 10.35RR116 pKa = 11.84NQRR119 pKa = 11.84IFSIYY124 pKa = 9.11TSEE127 pKa = 4.98KK128 pKa = 9.8NFGDD132 pKa = 3.26AKK134 pKa = 10.27EE135 pKa = 4.24SLRR138 pKa = 11.84RR139 pKa = 11.84AAMKK143 pKa = 10.08MPVSCKK149 pKa = 9.96IVIDD153 pKa = 3.99EE154 pKa = 4.21GAEE157 pKa = 3.98LVKK160 pKa = 11.05

Molecular weight: 18.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1920

0

1920

604930

29

6034

315.1

35.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.298 ± 0.061

0.909 ± 0.027

5.617 ± 0.064

6.491 ± 0.133

4.211 ± 0.041

6.573 ± 0.1

1.523 ± 0.028

10.254 ± 0.056

8.491 ± 0.156

8.373 ± 0.085

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.93 ± 0.036

8.722 ± 0.25

2.864 ± 0.05

1.989 ± 0.04

2.776 ± 0.058

7.21 ± 0.094

5.955 ± 0.14

6.309 ± 0.084

0.656 ± 0.016

3.848 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski