Methanobrevibacter filiformis
Average proteome isoelectric point is 6.36
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1920 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A166FFZ0|A0A166FFZ0_9EURY Uncharacterized protein OS=Methanobrevibacter filiformis OX=55758 GN=MBFIL_00230 PE=4 SV=1
MM1 pKa = 8.21 AEE3 pKa = 3.81 EE4 pKa = 5.8 DD5 pKa = 3.82 YY6 pKa = 11.6 DD7 pKa = 4.34 FDD9 pKa = 5.36 EE10 pKa = 4.57 EE11 pKa = 4.8 TEE13 pKa = 4.56 DD14 pKa = 3.59 SLKK17 pKa = 10.38 MFTGSVIDD25 pKa = 3.38 IQYY28 pKa = 10.97 LVDD31 pKa = 5.75 NMFLDD36 pKa = 4.11 YY37 pKa = 10.59 KK38 pKa = 11.15 SKK40 pKa = 11.07 FEE42 pKa = 4.07 NQEE45 pKa = 3.96 SPISYY50 pKa = 10.06 LDD52 pKa = 3.35 EE53 pKa = 6.28 GGDD56 pKa = 3.52 ARR58 pKa = 11.84 NLFEE62 pKa = 5.08 TISLPLFEE70 pKa = 5.35 IFWLINEE77 pKa = 4.44 SVLMKK82 pKa = 9.06 YY83 pKa = 9.89 IPYY86 pKa = 10.52 ASGDD90 pKa = 3.57 FLDD93 pKa = 5.06 ILGHH97 pKa = 5.78 NNPRR101 pKa = 11.84 NPGKK105 pKa = 10.38 NSTGQLQFSLPHH117 pKa = 6.96 DD118 pKa = 3.65 VLKK121 pKa = 10.84 DD122 pKa = 3.53 YY123 pKa = 10.98 DD124 pKa = 3.84 ITIPAYY130 pKa = 10.07 SVYY133 pKa = 9.82 LTEE136 pKa = 6.15 DD137 pKa = 3.64 DD138 pKa = 5.49 LGLEE142 pKa = 4.27 YY143 pKa = 9.82 EE144 pKa = 4.64 TIEE147 pKa = 4.64 EE148 pKa = 4.8 AILPAGEE155 pKa = 4.05 NSILVPARR163 pKa = 11.84 SVFGGSEE170 pKa = 4.03 YY171 pKa = 11.01 NIDD174 pKa = 3.91 SNLVTIFEE182 pKa = 4.19 EE183 pKa = 4.93 SIDD186 pKa = 3.98 DD187 pKa = 4.26 LLVTNPVPFIGGTDD201 pKa = 3.76 DD202 pKa = 5.64 EE203 pKa = 5.78 DD204 pKa = 4.71 DD205 pKa = 3.62 EE206 pKa = 5.11 YY207 pKa = 11.93 YY208 pKa = 10.38 RR209 pKa = 11.84 ARR211 pKa = 11.84 LLYY214 pKa = 9.43 EE215 pKa = 3.78 QSEE218 pKa = 4.46 HH219 pKa = 7.55 DD220 pKa = 3.82 FGSVGWYY227 pKa = 9.92 KK228 pKa = 10.79 RR229 pKa = 11.84 IAVKK233 pKa = 10.39 IPGVHH238 pKa = 6.29 DD239 pKa = 3.69 VKK241 pKa = 11.24 VINCLKK247 pKa = 10.23 GDD249 pKa = 3.73 YY250 pKa = 10.14 TIGIIVNPPTAEE262 pKa = 3.38 IVQNVTNFFNTADD275 pKa = 3.72 TTPAGINAYY284 pKa = 9.38 IYY286 pKa = 10.38 GAEE289 pKa = 4.19 TFPVDD294 pKa = 5.5 IIIQDD299 pKa = 3.76 LVFTNEE305 pKa = 3.69 VNPADD310 pKa = 3.73 VVEE313 pKa = 5.21 EE314 pKa = 4.01 IQNQINKK321 pKa = 9.33 YY322 pKa = 9.77 FNNLNISEE330 pKa = 4.49 TIYY333 pKa = 10.83 RR334 pKa = 11.84 NSILSILSNINGLIDD349 pKa = 3.56 YY350 pKa = 7.46 TLISPSVNIDD360 pKa = 3.22 AEE362 pKa = 4.44 EE363 pKa = 4.1 NQVLIQGEE371 pKa = 4.45 TIINN375 pKa = 3.56
Molecular weight: 42.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.727
IPC2_protein 3.821
IPC_protein 3.834
Toseland 3.617
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.706
Rodwell 3.656
Grimsley 3.528
Solomon 3.795
Lehninger 3.757
Nozaki 3.91
DTASelect 4.113
Thurlkill 3.656
EMBOSS 3.719
Sillero 3.948
Patrickios 0.884
IPC_peptide 3.795
IPC2_peptide 3.923
IPC2.peptide.svr19 3.832
Protein with the highest isoelectric point:
>tr|A0A166ESV9|A0A166ESV9_9EURY Modification methylase HaeIII OS=Methanobrevibacter filiformis OX=55758 GN=haeIIIM PE=3 SV=1
MM1 pKa = 6.68 VRR3 pKa = 11.84 AYY5 pKa = 10.52 SRR7 pKa = 11.84 RR8 pKa = 11.84 EE9 pKa = 3.95 YY10 pKa = 10.02 IRR12 pKa = 11.84 KK13 pKa = 9.13 IPGSRR18 pKa = 11.84 IVQYY22 pKa = 11.77 DD23 pKa = 3.51 MGNLSQRR30 pKa = 11.84 FPVAVSLAVKK40 pKa = 8.41 TPARR44 pKa = 11.84 ITHH47 pKa = 6.32 NALEE51 pKa = 4.49 AGRR54 pKa = 11.84 VASNRR59 pKa = 11.84 FMQRR63 pKa = 11.84 KK64 pKa = 8.84 AGRR67 pKa = 11.84 LGYY70 pKa = 9.36 HH71 pKa = 6.23 LKK73 pKa = 10.25 IRR75 pKa = 11.84 VYY77 pKa = 7.96 PHH79 pKa = 6.37 NIIRR83 pKa = 11.84 EE84 pKa = 4.11 NPMATGAGADD94 pKa = 3.36 RR95 pKa = 11.84 VQDD98 pKa = 3.52 GMRR101 pKa = 11.84 KK102 pKa = 9.49 AFGKK106 pKa = 10.01 SVSSEE111 pKa = 3.7 AVVKK115 pKa = 10.35 RR116 pKa = 11.84 NQRR119 pKa = 11.84 IFSIYY124 pKa = 9.11 TSEE127 pKa = 4.98 KK128 pKa = 9.8 NFGDD132 pKa = 3.26 AKK134 pKa = 10.27 EE135 pKa = 4.24 SLRR138 pKa = 11.84 RR139 pKa = 11.84 AAMKK143 pKa = 10.08 MPVSCKK149 pKa = 9.96 IVIDD153 pKa = 3.99 EE154 pKa = 4.21 GAEE157 pKa = 3.98 LVKK160 pKa = 11.05
Molecular weight: 18.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.326
IPC2_protein 9.897
IPC_protein 10.774
Toseland 10.906
ProMoST 10.687
Dawson 10.979
Bjellqvist 10.716
Wikipedia 11.213
Rodwell 11.199
Grimsley 11.023
Solomon 11.14
Lehninger 11.096
Nozaki 10.877
DTASelect 10.716
Thurlkill 10.891
EMBOSS 11.316
Sillero 10.921
Patrickios 10.921
IPC_peptide 11.14
IPC2_peptide 9.648
IPC2.peptide.svr19 8.461
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1920
0
1920
604930
29
6034
315.1
35.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.298 ± 0.061
0.909 ± 0.027
5.617 ± 0.064
6.491 ± 0.133
4.211 ± 0.041
6.573 ± 0.1
1.523 ± 0.028
10.254 ± 0.056
8.491 ± 0.156
8.373 ± 0.085
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.93 ± 0.036
8.722 ± 0.25
2.864 ± 0.05
1.989 ± 0.04
2.776 ± 0.058
7.21 ± 0.094
5.955 ± 0.14
6.309 ± 0.084
0.656 ± 0.016
3.848 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here