Amino acid dipepetide frequency for Methanobrevibacter filiformis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.942AlaAla: 2.942 ± 0.117
0.562AlaCys: 0.562 ± 0.035
2.564AlaAsp: 2.564 ± 0.087
3.085AlaGlu: 3.085 ± 0.092
1.794AlaPhe: 1.794 ± 0.065
3.566AlaGly: 3.566 ± 0.109
0.817AlaHis: 0.817 ± 0.04
7.591AlaIle: 7.591 ± 0.164
4.412AlaLys: 4.412 ± 0.122
4.369AlaLeu: 4.369 ± 0.099
1.151AlaMet: 1.151 ± 0.048
3.789AlaAsn: 3.789 ± 0.184
1.44AlaPro: 1.44 ± 0.054
1.055AlaGln: 1.055 ± 0.047
1.569AlaArg: 1.569 ± 0.063
3.597AlaSer: 3.597 ± 0.144
3.432AlaThr: 3.432 ± 0.174
3.433AlaVal: 3.433 ± 0.102
0.251AlaTrp: 0.251 ± 0.018
1.559AlaTyr: 1.559 ± 0.051
0.0AlaXaa: 0.0 ± 0.0
Cys
0.478CysAla: 0.478 ± 0.034
0.131CysCys: 0.131 ± 0.017
0.565CysAsp: 0.565 ± 0.035
0.615CysGlu: 0.615 ± 0.039
0.324CysPhe: 0.324 ± 0.024
1.081CysGly: 1.081 ± 0.053
0.207CysHis: 0.207 ± 0.021
0.689CysIle: 0.689 ± 0.046
0.676CysLys: 0.676 ± 0.035
0.622CysLeu: 0.622 ± 0.034
0.183CysMet: 0.183 ± 0.017
0.57CysAsn: 0.57 ± 0.04
0.603CysPro: 0.603 ± 0.052
0.185CysGln: 0.185 ± 0.016
0.264CysArg: 0.264 ± 0.023
0.61CysSer: 0.61 ± 0.037
0.395CysThr: 0.395 ± 0.025
0.569CysVal: 0.569 ± 0.037
0.051CysTrp: 0.051 ± 0.009
0.273CysTyr: 0.273 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
2.703AspAla: 2.703 ± 0.076
0.46AspCys: 0.46 ± 0.03
3.035AspAsp: 3.035 ± 0.092
4.402AspGlu: 4.402 ± 0.105
2.762AspPhe: 2.762 ± 0.077
3.207AspGly: 3.207 ± 0.11
0.767AspHis: 0.767 ± 0.036
6.021AspIle: 6.021 ± 0.143
4.733AspLys: 4.733 ± 0.122
5.26AspLeu: 5.26 ± 0.123
1.246AspMet: 1.246 ± 0.055
4.601AspAsn: 4.601 ± 0.14
1.775AspPro: 1.775 ± 0.074
0.992AspGln: 0.992 ± 0.044
1.271AspArg: 1.271 ± 0.048
3.698AspSer: 3.698 ± 0.099
2.551AspThr: 2.551 ± 0.078
3.776AspVal: 3.776 ± 0.087
0.395AspTrp: 0.395 ± 0.028
2.518AspTyr: 2.518 ± 0.077
0.0AspXaa: 0.0 ± 0.0
Glu
2.941GluAla: 2.941 ± 0.087
0.507GluCys: 0.507 ± 0.033
4.058GluAsp: 4.058 ± 0.12
5.401GluGlu: 5.401 ± 0.171
3.035GluPhe: 3.035 ± 0.084
3.407GluGly: 3.407 ± 0.095
0.969GluHis: 0.969 ± 0.049
7.098GluIle: 7.098 ± 0.168
6.305GluLys: 6.305 ± 0.166
5.971GluLeu: 5.971 ± 0.159
1.337GluMet: 1.337 ± 0.062
5.726GluAsn: 5.726 ± 0.115
1.289GluPro: 1.289 ± 0.059
1.091GluGln: 1.091 ± 0.058
1.807GluArg: 1.807 ± 0.063
3.87GluSer: 3.87 ± 0.106
3.176GluThr: 3.176 ± 0.095
3.915GluVal: 3.915 ± 0.115
0.362GluTrp: 0.362 ± 0.028
2.643GluTyr: 2.643 ± 0.104
0.0GluXaa: 0.0 ± 0.0
Phe
2.093PheAla: 2.093 ± 0.063
0.36PheCys: 0.36 ± 0.026
2.661PheAsp: 2.661 ± 0.07
2.539PheGlu: 2.539 ± 0.072
1.526PhePhe: 1.526 ± 0.064
2.541PheGly: 2.541 ± 0.079
0.635PheHis: 0.635 ± 0.032
4.331PheIle: 4.331 ± 0.125
4.01PheLys: 4.01 ± 0.105
3.518PheLeu: 3.518 ± 0.119
0.84PheMet: 0.84 ± 0.04
3.604PheAsn: 3.604 ± 0.131
1.091PhePro: 1.091 ± 0.054
0.903PheGln: 0.903 ± 0.042
1.121PheArg: 1.121 ± 0.052
3.217PheSer: 3.217 ± 0.108
2.711PheThr: 2.711 ± 0.11
2.638PheVal: 2.638 ± 0.087
0.195PheTrp: 0.195 ± 0.019
1.574PheTyr: 1.574 ± 0.065
0.0PheXaa: 0.0 ± 0.0
Gly
4.779GlyAla: 4.779 ± 0.205
0.683GlyCys: 0.683 ± 0.044
3.625GlyAsp: 3.625 ± 0.099
3.791GlyGlu: 3.791 ± 0.09
2.754GlyPhe: 2.754 ± 0.081
5.187GlyGly: 5.187 ± 0.25
0.964GlyHis: 0.964 ± 0.042
6.452GlyIle: 6.452 ± 0.15
4.93GlyLys: 4.93 ± 0.121
5.108GlyLeu: 5.108 ± 0.106
1.233GlyMet: 1.233 ± 0.047
5.001GlyAsn: 5.001 ± 0.262
1.374GlyPro: 1.374 ± 0.063
1.258GlyGln: 1.258 ± 0.05
2.025GlyArg: 2.025 ± 0.063
4.796GlySer: 4.796 ± 0.138
4.329GlyThr: 4.329 ± 0.183
4.779GlyVal: 4.779 ± 0.15
0.403GlyTrp: 0.403 ± 0.029
2.26GlyTyr: 2.26 ± 0.074
0.0GlyXaa: 0.0 ± 0.0
His
0.709HisAla: 0.709 ± 0.035
0.212HisCys: 0.212 ± 0.02
0.921HisAsp: 0.921 ± 0.046
0.998HisGlu: 0.998 ± 0.042
0.67HisPhe: 0.67 ± 0.034
1.149HisGly: 1.149 ± 0.052
0.35HisHis: 0.35 ± 0.026
1.375HisIle: 1.375 ± 0.049
1.003HisLys: 1.003 ± 0.043
1.321HisLeu: 1.321 ± 0.055
0.327HisMet: 0.327 ± 0.025
0.941HisAsn: 0.941 ± 0.046
0.653HisPro: 0.653 ± 0.037
0.37HisGln: 0.37 ± 0.027
0.494HisArg: 0.494 ± 0.03
1.027HisSer: 1.027 ± 0.042
0.832HisThr: 0.832 ± 0.052
1.078HisVal: 1.078 ± 0.048
0.106HisTrp: 0.106 ± 0.013
0.699HisTyr: 0.699 ± 0.037
0.0HisXaa: 0.0 ± 0.0
Ile
7.13IleAla: 7.13 ± 0.141
0.921IleCys: 0.921 ± 0.046
5.863IleAsp: 5.863 ± 0.117
6.736IleGlu: 6.736 ± 0.163
4.162IlePhe: 4.162 ± 0.105
6.432IleGly: 6.432 ± 0.136
1.749IleHis: 1.749 ± 0.057
10.317IleIle: 10.317 ± 0.219
8.251IleLys: 8.251 ± 0.155
8.371IleLeu: 8.371 ± 0.194
1.81IleMet: 1.81 ± 0.067
8.763IleAsn: 8.763 ± 0.293
3.435IlePro: 3.435 ± 0.1
1.987IleGln: 1.987 ± 0.071
2.673IleArg: 2.673 ± 0.083
7.244IleSer: 7.244 ± 0.128
6.298IleThr: 6.298 ± 0.19
5.918IleVal: 5.918 ± 0.107
0.593IleTrp: 0.593 ± 0.045
3.88IleTyr: 3.88 ± 0.14
0.0IleXaa: 0.0 ± 0.0
Lys
3.938LysAla: 3.938 ± 0.107
0.712LysCys: 0.712 ± 0.048
4.862LysAsp: 4.862 ± 0.126
7.224LysGlu: 7.224 ± 0.205
3.25LysPhe: 3.25 ± 0.089
4.477LysGly: 4.477 ± 0.118
1.334LysHis: 1.334 ± 0.055
9.15LysIle: 9.15 ± 0.186
7.984LysLys: 7.984 ± 0.208
7.308LysLeu: 7.308 ± 0.154
1.739LysMet: 1.739 ± 0.062
6.847LysAsn: 6.847 ± 0.166
2.099LysPro: 2.099 ± 0.069
1.837LysGln: 1.837 ± 0.069
2.579LysArg: 2.579 ± 0.098
5.427LysSer: 5.427 ± 0.136
4.931LysThr: 4.931 ± 0.125
4.716LysVal: 4.716 ± 0.114
0.749LysTrp: 0.749 ± 0.041
3.749LysTyr: 3.749 ± 0.101
0.0LysXaa: 0.0 ± 0.0
Leu
4.371LeuAla: 4.371 ± 0.116
0.658LeuCys: 0.658 ± 0.043
5.477LeuAsp: 5.477 ± 0.109
5.634LeuGlu: 5.634 ± 0.139
3.534LeuPhe: 3.534 ± 0.107
5.063LeuGly: 5.063 ± 0.15
1.17LeuHis: 1.17 ± 0.051
8.365LeuIle: 8.365 ± 0.191
8.389LeuLys: 8.389 ± 0.209
6.687LeuLeu: 6.687 ± 0.192
1.77LeuMet: 1.77 ± 0.065
7.34LeuAsn: 7.34 ± 0.151
2.437LeuPro: 2.437 ± 0.07
1.518LeuGln: 1.518 ± 0.059
2.476LeuArg: 2.476 ± 0.08
6.186LeuSer: 6.186 ± 0.138
4.844LeuThr: 4.844 ± 0.11
4.678LeuVal: 4.678 ± 0.124
0.512LeuTrp: 0.512 ± 0.028
2.62LeuTyr: 2.62 ± 0.083
0.0LeuXaa: 0.0 ± 0.0
Met
1.104MetAla: 1.104 ± 0.049
0.193MetCys: 0.193 ± 0.018
1.394MetAsp: 1.394 ± 0.059
1.451MetGlu: 1.451 ± 0.066
0.742MetPhe: 0.742 ± 0.038
1.278MetGly: 1.278 ± 0.052
0.294MetHis: 0.294 ± 0.025
1.856MetIle: 1.856 ± 0.073
1.893MetLys: 1.893 ± 0.064
1.524MetLeu: 1.524 ± 0.061
0.431MetMet: 0.431 ± 0.029
1.324MetAsn: 1.324 ± 0.056
0.625MetPro: 0.625 ± 0.035
0.342MetGln: 0.342 ± 0.026
0.613MetArg: 0.613 ± 0.039
1.375MetSer: 1.375 ± 0.052
0.927MetThr: 0.927 ± 0.045
1.294MetVal: 1.294 ± 0.055
0.121MetTrp: 0.121 ± 0.015
0.521MetTyr: 0.521 ± 0.032
0.0MetXaa: 0.0 ± 0.0
Asn
3.85AsnAla: 3.85 ± 0.218
0.694AsnCys: 0.694 ± 0.049
3.739AsnAsp: 3.739 ± 0.094
4.596AsnGlu: 4.596 ± 0.112
3.648AsnPhe: 3.648 ± 0.179
5.657AsnGly: 5.657 ± 0.325
1.284AsnHis: 1.284 ± 0.072
8.76AsnIle: 8.76 ± 0.188
6.91AsnLys: 6.91 ± 0.155
6.621AsnLeu: 6.621 ± 0.136
1.369AsnMet: 1.369 ± 0.054
11.021AsnAsn: 11.021 ± 0.612
2.6AsnPro: 2.6 ± 0.079
1.719AsnGln: 1.719 ± 0.091
1.982AsnArg: 1.982 ± 0.059
7.662AsnSer: 7.662 ± 0.387
5.363AsnThr: 5.363 ± 0.3
5.627AsnVal: 5.627 ± 0.303
0.623AsnTrp: 0.623 ± 0.044
3.5AsnTyr: 3.5 ± 0.153
0.0AsnXaa: 0.0 ± 0.0
Pro
1.289ProAla: 1.289 ± 0.057
0.312ProCys: 0.312 ± 0.023
1.656ProAsp: 1.656 ± 0.06
2.222ProGlu: 2.222 ± 0.074
1.222ProPhe: 1.222 ± 0.053
1.721ProGly: 1.721 ± 0.063
0.529ProHis: 0.529 ± 0.029
2.886ProIle: 2.886 ± 0.08
2.422ProLys: 2.422 ± 0.083
2.503ProLeu: 2.503 ± 0.083
0.534ProMet: 0.534 ± 0.032
1.926ProAsn: 1.926 ± 0.065
0.754ProPro: 0.754 ± 0.04
0.731ProGln: 0.731 ± 0.043
0.896ProArg: 0.896 ± 0.044
1.936ProSer: 1.936 ± 0.069
1.569ProThr: 1.569 ± 0.06
2.109ProVal: 2.109 ± 0.069
0.19ProTrp: 0.19 ± 0.017
1.235ProTyr: 1.235 ± 0.051
0.0ProXaa: 0.0 ± 0.0
Gln
1.065GlnAla: 1.065 ± 0.046
0.198GlnCys: 0.198 ± 0.021
1.068GlnAsp: 1.068 ± 0.047
1.41GlnGlu: 1.41 ± 0.054
0.807GlnPhe: 0.807 ± 0.047
1.283GlnGly: 1.283 ± 0.046
0.329GlnHis: 0.329 ± 0.022
2.086GlnIle: 2.086 ± 0.067
1.787GlnLys: 1.787 ± 0.075
1.741GlnLeu: 1.741 ± 0.065
0.456GlnMet: 0.456 ± 0.03
1.433GlnAsn: 1.433 ± 0.055
0.491GlnPro: 0.491 ± 0.033
0.489GlnGln: 0.489 ± 0.036
0.666GlnArg: 0.666 ± 0.037
1.25GlnSer: 1.25 ± 0.059
1.175GlnThr: 1.175 ± 0.054
1.113GlnVal: 1.113 ± 0.052
0.212GlnTrp: 0.212 ± 0.021
0.833GlnTyr: 0.833 ± 0.041
0.0GlnXaa: 0.0 ± 0.0
Arg
1.499ArgAla: 1.499 ± 0.064
0.283ArgCys: 0.283 ± 0.026
1.561ArgAsp: 1.561 ± 0.06
2.086ArgGlu: 2.086 ± 0.087
1.278ArgPhe: 1.278 ± 0.054
1.868ArgGly: 1.868 ± 0.066
0.379ArgHis: 0.379 ± 0.028
2.747ArgIle: 2.747 ± 0.096
2.445ArgLys: 2.445 ± 0.091
2.341ArgLeu: 2.341 ± 0.078
0.636ArgMet: 0.636 ± 0.036
1.918ArgAsn: 1.918 ± 0.055
0.873ArgPro: 0.873 ± 0.037
0.64ArgGln: 0.64 ± 0.029
1.164ArgArg: 1.164 ± 0.059
1.752ArgSer: 1.752 ± 0.066
1.384ArgThr: 1.384 ± 0.052
1.628ArgVal: 1.628 ± 0.06
0.217ArgTrp: 0.217 ± 0.024
1.058ArgTyr: 1.058 ± 0.05
0.0ArgXaa: 0.0 ± 0.0
Ser
3.567SerAla: 3.567 ± 0.134
0.588SerCys: 0.588 ± 0.035
3.336SerAsp: 3.336 ± 0.089
3.807SerGlu: 3.807 ± 0.107
3.372SerPhe: 3.372 ± 0.104
5.174SerGly: 5.174 ± 0.161
1.035SerHis: 1.035 ± 0.045
6.66SerIle: 6.66 ± 0.122
6.295SerLys: 6.295 ± 0.139
5.807SerLeu: 5.807 ± 0.145
1.313SerMet: 1.313 ± 0.044
6.756SerAsn: 6.756 ± 0.288
1.848SerPro: 1.848 ± 0.069
1.506SerGln: 1.506 ± 0.054
1.926SerArg: 1.926 ± 0.059
6.277SerSer: 6.277 ± 0.282
4.718SerThr: 4.718 ± 0.205
4.639SerVal: 4.639 ± 0.182
0.479SerTrp: 0.479 ± 0.029
2.997SerTyr: 2.997 ± 0.087
0.0SerXaa: 0.0 ± 0.0
Thr
3.19ThrAla: 3.19 ± 0.126
0.491ThrCys: 0.491 ± 0.033
3.131ThrAsp: 3.131 ± 0.109
2.774ThrGlu: 2.774 ± 0.091
2.574ThrPhe: 2.574 ± 0.089
5.047ThrGly: 5.047 ± 0.253
0.886ThrHis: 0.886 ± 0.043
6.554ThrIle: 6.554 ± 0.259
4.123ThrLys: 4.123 ± 0.09
5.42ThrLeu: 5.42 ± 0.163
0.896ThrMet: 0.896 ± 0.039
5.321ThrAsn: 5.321 ± 0.292
1.992ThrPro: 1.992 ± 0.073
1.113ThrGln: 1.113 ± 0.044
1.4ThrArg: 1.4 ± 0.065
3.86ThrSer: 3.86 ± 0.096
3.969ThrThr: 3.969 ± 0.178
4.197ThrVal: 4.197 ± 0.179
0.307ThrTrp: 0.307 ± 0.024
2.308ThrTyr: 2.308 ± 0.118
0.0ThrXaa: 0.0 ± 0.0
Val
3.4ValAla: 3.4 ± 0.109
0.622ValCys: 0.622 ± 0.037
3.748ValAsp: 3.748 ± 0.092
3.743ValGlu: 3.743 ± 0.115
2.549ValPhe: 2.549 ± 0.066
4.164ValGly: 4.164 ± 0.109
0.827ValHis: 0.827 ± 0.041
5.903ValIle: 5.903 ± 0.122
5.03ValLys: 5.03 ± 0.096
5.229ValLeu: 5.229 ± 0.132
1.136ValMet: 1.136 ± 0.057
5.913ValAsn: 5.913 ± 0.348
1.985ValPro: 1.985 ± 0.075
1.212ValGln: 1.212 ± 0.057
1.608ValArg: 1.608 ± 0.056
4.93ValSer: 4.93 ± 0.172
4.118ValThr: 4.118 ± 0.255
4.447ValVal: 4.447 ± 0.161
0.311ValTrp: 0.311 ± 0.026
2.218ValTyr: 2.218 ± 0.077
0.0ValXaa: 0.0 ± 0.0
Trp
0.299TrpAla: 0.299 ± 0.022
0.071TrpCys: 0.071 ± 0.011
0.446TrpAsp: 0.446 ± 0.032
0.382TrpGlu: 0.382 ± 0.025
0.321TrpPhe: 0.321 ± 0.024
0.468TrpGly: 0.468 ± 0.029
0.111TrpHis: 0.111 ± 0.012
0.546TrpIle: 0.546 ± 0.031
0.521TrpLys: 0.521 ± 0.033
0.465TrpLeu: 0.465 ± 0.03
0.16TrpMet: 0.16 ± 0.018
0.636TrpAsn: 0.636 ± 0.053
0.106TrpPro: 0.106 ± 0.015
0.162TrpGln: 0.162 ± 0.017
0.21TrpArg: 0.21 ± 0.02
0.425TrpSer: 0.425 ± 0.038
0.471TrpThr: 0.471 ± 0.048
0.344TrpVal: 0.344 ± 0.025
0.137TrpTrp: 0.137 ± 0.017
0.283TrpTyr: 0.283 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.628TyrAla: 1.628 ± 0.056
0.433TyrCys: 0.433 ± 0.028
2.461TyrAsp: 2.461 ± 0.073
2.013TyrGlu: 2.013 ± 0.086
1.784TyrPhe: 1.784 ± 0.068
2.961TyrGly: 2.961 ± 0.098
0.6TyrHis: 0.6 ± 0.034
3.147TyrIle: 3.147 ± 0.09
2.794TyrLys: 2.794 ± 0.087
3.544TyrLeu: 3.544 ± 0.097
0.734TyrMet: 0.734 ± 0.042
3.795TyrAsn: 3.795 ± 0.185
1.17TyrPro: 1.17 ± 0.049
0.784TyrGln: 0.784 ± 0.041
1.01TyrArg: 1.01 ± 0.043
2.964TyrSer: 2.964 ± 0.11
2.362TyrThr: 2.362 ± 0.126
2.194TyrVal: 2.194 ± 0.086
0.347TyrTrp: 0.347 ± 0.024
1.749TyrTyr: 1.749 ± 0.097
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1920 proteins (604930 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski