Fasciola hepatica (Liver fluke)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Spiralia; Lophotrochozoa; Platyhelminthes; Trematoda; Digenea; Plagiorchiida; Echinostomata; Echinostomatoidea; Fasciolidae; Fasciola

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11190 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4E0RN80|A0A4E0RN80_FASHE Glutamine rich 2 OS=Fasciola hepatica OX=6192 GN=D915_000008 PE=4 SV=1
MM1 pKa = 7.45FYY3 pKa = 11.23ARR5 pKa = 11.84FVSDD9 pKa = 6.03DD10 pKa = 3.48NDD12 pKa = 4.89DD13 pKa = 5.69DD14 pKa = 5.07DD15 pKa = 6.42GCDD18 pKa = 3.86ADD20 pKa = 5.8CIDD23 pKa = 3.67NVVAVRR29 pKa = 11.84HH30 pKa = 5.62MYY32 pKa = 10.62GNHH35 pKa = 7.01PDD37 pKa = 3.55YY38 pKa = 11.03LLRR41 pKa = 11.84EE42 pKa = 4.22CDD44 pKa = 3.92VLDD47 pKa = 4.55FDD49 pKa = 5.73PEE51 pKa = 4.27TSTITVIVRR60 pKa = 11.84VAEE63 pKa = 4.08KK64 pKa = 10.22TLMCSAADD72 pKa = 3.89PEE74 pKa = 4.41DD75 pKa = 3.9VDD77 pKa = 6.38LIWTSS82 pKa = 3.18

Molecular weight: 9.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4E0S2M7|A0A4E0S2M7_FASHE Uncharacterized protein OS=Fasciola hepatica OX=6192 GN=D915_001386 PE=4 SV=1
MM1 pKa = 7.63SSHH4 pKa = 5.7KK5 pKa = 8.49TWRR8 pKa = 11.84TKK10 pKa = 10.0TKK12 pKa = 10.32LGKK15 pKa = 9.53KK16 pKa = 8.91QKK18 pKa = 9.03QNRR21 pKa = 11.84TLPHH25 pKa = 5.31WTRR28 pKa = 11.84LRR30 pKa = 11.84TGNKK34 pKa = 8.34IRR36 pKa = 11.84YY37 pKa = 5.83NAKK40 pKa = 8.24RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.11WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 11.06LNLL51 pKa = 3.44

Molecular weight: 6.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11190

0

11190

6088080

26

8454

544.1

60.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.715 ± 0.018

2.091 ± 0.013

5.249 ± 0.013

5.794 ± 0.028

3.637 ± 0.017

5.502 ± 0.026

2.744 ± 0.01

4.738 ± 0.016

4.372 ± 0.022

9.641 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.123 ± 0.008

4.335 ± 0.013

5.914 ± 0.026

4.387 ± 0.017

6.471 ± 0.02

9.642 ± 0.036

6.562 ± 0.019

6.336 ± 0.015

1.139 ± 0.007

2.608 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski