Aminivibrio pyruvatiphilus
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2990 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4R8M2W7|A0A4R8M2W7_9BACT Amino acid/amide ABC transporter membrane protein 1 (HAAT family) OS=Aminivibrio pyruvatiphilus OX=1005740 GN=C8D99_11357 PE=4 SV=1
MM1 pKa = 7.51 KK2 pKa = 10.22 KK3 pKa = 10.16 YY4 pKa = 10.18 VCTVCGYY11 pKa = 10.9 VYY13 pKa = 10.63 DD14 pKa = 4.41 PAEE17 pKa = 4.67 GDD19 pKa = 3.67 PDD21 pKa = 4.0 SGVAPGTAFEE31 pKa = 5.97 DD32 pKa = 4.28 IPDD35 pKa = 3.7 DD36 pKa = 3.88 WVCPVCAVGKK46 pKa = 10.57 DD47 pKa = 3.28 MFEE50 pKa = 4.35 PEE52 pKa = 4.01 EE53 pKa = 3.99
Molecular weight: 5.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.869
IPC2_protein 3.706
IPC_protein 3.605
Toseland 3.414
ProMoST 3.757
Dawson 3.617
Bjellqvist 3.884
Wikipedia 3.592
Rodwell 3.452
Grimsley 3.338
Solomon 3.579
Lehninger 3.541
Nozaki 3.77
DTASelect 3.948
Thurlkill 3.503
EMBOSS 3.592
Sillero 3.745
Patrickios 0.121
IPC_peptide 3.579
IPC2_peptide 3.706
IPC2.peptide.svr19 3.774
Protein with the highest isoelectric point:
>tr|A0A4R8MGU1|A0A4R8MGU1_9BACT 2-methylcitrate dehydratase PrpD OS=Aminivibrio pyruvatiphilus OX=1005740 GN=C8D99_102126 PE=3 SV=1
MM1 pKa = 7.23 NRR3 pKa = 11.84 LNGKK7 pKa = 9.79 LSGGRR12 pKa = 11.84 IRR14 pKa = 11.84 PRR16 pKa = 11.84 SFFEE20 pKa = 3.88 PPRR23 pKa = 11.84 AEE25 pKa = 4.41 DD26 pKa = 3.91 LPTVSLQMKK35 pKa = 9.09 GRR37 pKa = 11.84 KK38 pKa = 8.65 FDD40 pKa = 3.7 PGMVIGTARR49 pKa = 11.84 RR50 pKa = 11.84 CVFGLPSVIVCSPLRR65 pKa = 11.84 DD66 pKa = 3.49 GKK68 pKa = 9.91 PFPTTFWLTCPHH80 pKa = 6.65 LVKK83 pKa = 10.88 LCGALEE89 pKa = 4.25 SEE91 pKa = 4.67 GAVAEE96 pKa = 4.14 LEE98 pKa = 4.16 RR99 pKa = 11.84 RR100 pKa = 11.84 RR101 pKa = 11.84 MEE103 pKa = 5.71 RR104 pKa = 11.84 PDD106 pKa = 3.54 LWTEE110 pKa = 3.76 YY111 pKa = 11.15 NLFHH115 pKa = 7.29 ARR117 pKa = 11.84 LRR119 pKa = 11.84 LLLVQPAAARR129 pKa = 11.84 FLRR132 pKa = 11.84 KK133 pKa = 9.46 YY134 pKa = 10.49 RR135 pKa = 11.84 RR136 pKa = 11.84 GVWDD140 pKa = 3.35 TLRR143 pKa = 11.84 RR144 pKa = 11.84 GGAGGIDD151 pKa = 3.7 YY152 pKa = 10.61 LSRR155 pKa = 11.84 ARR157 pKa = 11.84 GGAKK161 pKa = 9.81 CLHH164 pKa = 6.29 LQAASWLALGFHH176 pKa = 7.12 PAGEE180 pKa = 4.39 FLHH183 pKa = 6.53 KK184 pKa = 10.72 ALVPLHH190 pKa = 6.44 CAAPAASGCVRR201 pKa = 11.84 RR202 pKa = 11.84 CC203 pKa = 3.03
Molecular weight: 22.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.502
IPC_protein 10.335
Toseland 10.687
ProMoST 10.423
Dawson 10.76
Bjellqvist 10.511
Wikipedia 10.994
Rodwell 10.877
Grimsley 10.804
Solomon 10.921
Lehninger 10.891
Nozaki 10.716
DTASelect 10.496
Thurlkill 10.687
EMBOSS 11.096
Sillero 10.716
Patrickios 10.613
IPC_peptide 10.935
IPC2_peptide 9.911
IPC2.peptide.svr19 8.621
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2990
0
2990
1007143
26
2953
336.8
36.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.837 ± 0.059
1.157 ± 0.018
4.934 ± 0.033
7.036 ± 0.048
4.396 ± 0.031
9.202 ± 0.045
1.604 ± 0.016
5.361 ± 0.04
4.458 ± 0.036
10.532 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.707 ± 0.022
2.688 ± 0.027
4.922 ± 0.033
2.234 ± 0.021
6.569 ± 0.042
6.381 ± 0.031
4.79 ± 0.029
7.541 ± 0.038
1.206 ± 0.016
2.445 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here