Tortoise microvirus 63
Average proteome isoelectric point is 5.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W7L1|A0A4P8W7L1_9VIRU Uncharacterized protein OS=Tortoise microvirus 63 OX=2583169 PE=4 SV=1
MM1 pKa = 7.39 YY2 pKa = 10.25 SVCLDD7 pKa = 3.75 DD8 pKa = 6.42 GSIIGCGLGDD18 pKa = 4.33 CYY20 pKa = 10.9 DD21 pKa = 3.42 VHH23 pKa = 8.53 LLIPHH28 pKa = 7.04 LMMDD32 pKa = 5.36 AIDD35 pKa = 3.91 NVLNGDD41 pKa = 3.9 TMTLQDD47 pKa = 5.69 FIRR50 pKa = 11.84 LACAEE55 pKa = 4.1 YY56 pKa = 11.13 LFICGDD62 pKa = 3.5 NYY64 pKa = 10.74 VPKK67 pKa = 10.11 TT68 pKa = 3.6
Molecular weight: 7.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.889
IPC2_protein 3.821
IPC_protein 3.757
Toseland 3.528
ProMoST 3.986
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.808
Rodwell 3.592
Grimsley 3.452
Solomon 3.77
Lehninger 3.719
Nozaki 3.935
DTASelect 4.24
Thurlkill 3.63
EMBOSS 3.808
Sillero 3.897
Patrickios 0.006
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.809
Protein with the highest isoelectric point:
>tr|A0A4P8W7B6|A0A4P8W7B6_9VIRU Uncharacterized protein OS=Tortoise microvirus 63 OX=2583169 PE=4 SV=1
MM1 pKa = 7.4 SCANPITITVKK12 pKa = 10.27 GRR14 pKa = 11.84 PVQVGCRR21 pKa = 11.84 HH22 pKa = 6.08 CLSCRR27 pKa = 11.84 LDD29 pKa = 3.49 YY30 pKa = 11.13 QIALEE35 pKa = 4.07 FAVKK39 pKa = 10.51 HH40 pKa = 5.43 EE41 pKa = 4.8 LFDD44 pKa = 3.99 IYY46 pKa = 11.03 QSGLGACFATLTYY59 pKa = 10.51 ADD61 pKa = 4.23 SALPPHH67 pKa = 6.51 GSLRR71 pKa = 11.84 KK72 pKa = 9.48 SDD74 pKa = 3.65 FQNFLKK80 pKa = 10.59 NVRR83 pKa = 11.84 IQAKK87 pKa = 9.95 RR88 pKa = 11.84 KK89 pKa = 7.72 GTLPPFKK96 pKa = 10.59 YY97 pKa = 9.83 LACGEE102 pKa = 4.31 YY103 pKa = 10.34 GDD105 pKa = 4.46 KK106 pKa = 10.53 FGRR109 pKa = 11.84 PHH111 pKa = 5.25 YY112 pKa = 10.13 HH113 pKa = 6.39 VIFLGVTDD121 pKa = 4.39 ALFNQFIRR129 pKa = 11.84 PLWKK133 pKa = 10.23 HH134 pKa = 5.72 GLCDD138 pKa = 4.08 CSTLGSGAIRR148 pKa = 11.84 YY149 pKa = 7.11 VLKK152 pKa = 10.9 YY153 pKa = 8.14 CTKK156 pKa = 10.54 SAMGSKK162 pKa = 10.03 AKK164 pKa = 10.21 EE165 pKa = 4.04 MYY167 pKa = 10.28 DD168 pKa = 3.65 DD169 pKa = 4.01 QSLTRR174 pKa = 11.84 PFIVHH179 pKa = 6.05 SKK181 pKa = 10.4 RR182 pKa = 11.84 LGFNWLIRR190 pKa = 11.84 NRR192 pKa = 11.84 DD193 pKa = 3.62 DD194 pKa = 4.76 LVANNFCYY202 pKa = 10.44 LNKK205 pKa = 9.83 GHH207 pKa = 7.02 RR208 pKa = 11.84 VPLPAYY214 pKa = 9.33 YY215 pKa = 10.0 RR216 pKa = 11.84 RR217 pKa = 11.84 QFDD220 pKa = 3.24 IYY222 pKa = 10.89 GTFCPLPQIQQLDD235 pKa = 3.89 AEE237 pKa = 4.97 AKK239 pKa = 7.73 QHH241 pKa = 6.29 GYY243 pKa = 9.49 PSYY246 pKa = 10.82 RR247 pKa = 11.84 EE248 pKa = 3.74 WSIQKK253 pKa = 10.22 AFNRR257 pKa = 11.84 EE258 pKa = 3.34 QDD260 pKa = 3.13 LTIAVRR266 pKa = 11.84 NDD268 pKa = 4.52 GIPVDD273 pKa = 4.8 SLITPNRR280 pKa = 11.84 GYY282 pKa = 10.99 SPLDD286 pKa = 3.33 TSIVPQVLNRR296 pKa = 11.84 SS297 pKa = 3.62
Molecular weight: 33.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.73
IPC2_protein 8.609
IPC_protein 8.507
Toseland 8.814
ProMoST 8.946
Dawson 9.282
Bjellqvist 9.311
Wikipedia 9.428
Rodwell 9.37
Grimsley 9.224
Solomon 9.355
Lehninger 9.311
Nozaki 9.414
DTASelect 9.151
Thurlkill 9.209
EMBOSS 9.399
Sillero 9.428
Patrickios 4.164
IPC_peptide 9.341
IPC2_peptide 8.361
IPC2.peptide.svr19 7.833
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1676
68
578
239.4
26.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.637 ± 1.396
1.372 ± 0.843
6.981 ± 0.811
4.057 ± 0.759
5.012 ± 0.574
6.146 ± 0.669
1.79 ± 0.294
4.893 ± 0.899
3.878 ± 1.222
8.652 ± 0.84
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.983 ± 0.458
4.654 ± 0.451
5.609 ± 0.709
5.43 ± 1.571
5.251 ± 0.468
9.069 ± 0.94
5.489 ± 0.923
5.489 ± 0.629
0.955 ± 0.143
4.654 ± 0.514
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here