Tomato pseudo-curly top virus (TPCTV)

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Topocuvirus

Average proteome isoelectric point is 7.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q88889|C2_TPCTV Protein C2 OS=Tomato pseudo-curly top virus OX=49267 GN=C2 PE=3 SV=1
MM1 pKa = 7.85PSNPKK6 pKa = 9.99RR7 pKa = 11.84FQIAAKK13 pKa = 10.09NYY15 pKa = 8.13FLTYY19 pKa = 9.42PNCSLSKK26 pKa = 11.06EE27 pKa = 4.09EE28 pKa = 6.13ALDD31 pKa = 3.41QLQRR35 pKa = 11.84LQTPTNKK42 pKa = 10.17KK43 pKa = 7.7YY44 pKa = 10.69IKK46 pKa = 9.88VARR49 pKa = 11.84EE50 pKa = 3.51LHH52 pKa = 6.43EE53 pKa = 4.62NGEE56 pKa = 4.28PHH58 pKa = 6.66LHH60 pKa = 6.03VLIQFEE66 pKa = 5.08GKK68 pKa = 10.06FNCKK72 pKa = 7.32NQRR75 pKa = 11.84FFDD78 pKa = 4.05LVSPTRR84 pKa = 11.84STHH87 pKa = 4.78FHH89 pKa = 6.87PNIQGAKK96 pKa = 9.58SSSDD100 pKa = 3.24VNSYY104 pKa = 9.77VDD106 pKa = 3.82KK107 pKa = 11.49DD108 pKa = 3.28GDD110 pKa = 3.95TIEE113 pKa = 4.07WGEE116 pKa = 3.82FQIDD120 pKa = 3.13ARR122 pKa = 11.84SARR125 pKa = 11.84GGQQTANDD133 pKa = 3.74EE134 pKa = 4.18CAEE137 pKa = 3.93ALNRR141 pKa = 11.84SSKK144 pKa = 10.97EE145 pKa = 3.45EE146 pKa = 3.49ALQIIKK152 pKa = 10.52EE153 pKa = 4.07KK154 pKa = 10.65LPKK157 pKa = 10.5DD158 pKa = 3.74FLFCYY163 pKa = 10.31HH164 pKa = 6.57NLVSNLDD171 pKa = 4.14RR172 pKa = 11.84IFTPAPTPFVPPFQLSSFTNVPEE195 pKa = 5.65DD196 pKa = 3.6MQEE199 pKa = 3.7WADD202 pKa = 3.87DD203 pKa = 3.96YY204 pKa = 11.59FGVSAAARR212 pKa = 11.84PMRR215 pKa = 11.84YY216 pKa = 9.6KK217 pKa = 10.74SIIIEE222 pKa = 4.3GEE224 pKa = 3.96SRR226 pKa = 11.84TGKK229 pKa = 8.58TMWARR234 pKa = 11.84SLGPHH239 pKa = 6.57NYY241 pKa = 10.17LSGHH245 pKa = 6.9LDD247 pKa = 3.48FNSRR251 pKa = 11.84VYY253 pKa = 11.25SNSALYY259 pKa = 10.53NVIDD263 pKa = 4.88DD264 pKa = 4.15VTPHH268 pKa = 5.44YY269 pKa = 10.89LKK271 pKa = 10.69LKK273 pKa = 9.09HH274 pKa = 5.7WKK276 pKa = 9.57EE277 pKa = 4.04LIGAQRR283 pKa = 11.84DD284 pKa = 3.53WQSNCKK290 pKa = 9.41YY291 pKa = 10.21GKK293 pKa = 9.18PVQIKK298 pKa = 10.19GGIPSIVLCNPGGDD312 pKa = 3.38TSFQDD317 pKa = 4.37FLDD320 pKa = 4.08KK321 pKa = 11.3EE322 pKa = 4.26EE323 pKa = 4.61NEE325 pKa = 4.27ALKK328 pKa = 10.83DD329 pKa = 3.27WTLYY333 pKa = 10.13NAVFIKK339 pKa = 9.85LTEE342 pKa = 3.99PLYY345 pKa = 11.14DD346 pKa = 3.49GTVV349 pKa = 2.82

Molecular weight: 39.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q88891|AC4_TPCTV Protein C4 OS=Tomato pseudo-curly top virus OX=49267 GN=C4 PE=3 SV=1
MM1 pKa = 8.27DD2 pKa = 4.3SRR4 pKa = 11.84TGEE7 pKa = 4.27LLTYY11 pKa = 7.18TQCQTGCYY19 pKa = 9.59FDD21 pKa = 6.0DD22 pKa = 4.24IKK24 pKa = 11.58NPIYY28 pKa = 10.53FRR30 pKa = 11.84LQNTTTMATGNKK42 pKa = 9.5IITLQIRR49 pKa = 11.84ANHH52 pKa = 6.09NLRR55 pKa = 11.84KK56 pKa = 10.04VLGLQICWLNLVVLSVSPRR75 pKa = 11.84LSGEE79 pKa = 3.86ALLDD83 pKa = 3.32RR84 pKa = 11.84FKK86 pKa = 11.18FHH88 pKa = 6.98VMKK91 pKa = 10.55HH92 pKa = 5.2LNNLGVISINNVIRR106 pKa = 11.84SINHH110 pKa = 6.77FIDD113 pKa = 5.26LFDD116 pKa = 3.38QRR118 pKa = 11.84VFYY121 pKa = 10.9APSFYY126 pKa = 11.26YY127 pKa = 9.76NIKK130 pKa = 8.76MRR132 pKa = 11.84LYY134 pKa = 10.62

Molecular weight: 15.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1065

85

349

177.5

20.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.822 ± 0.754

2.723 ± 0.563

4.789 ± 0.553

5.164 ± 1.327

4.038 ± 0.742

6.009 ± 0.541

2.347 ± 0.526

6.009 ± 0.582

6.197 ± 0.671

8.451 ± 0.845

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.784 ± 0.46

6.103 ± 0.738

5.258 ± 0.856

3.944 ± 0.409

6.009 ± 0.602

8.545 ± 0.906

6.291 ± 0.943

4.789 ± 1.026

1.315 ± 0.213

4.413 ± 0.675
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski