Romboutsia lituseburensis DSM 797

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae; Romboutsia; Romboutsia lituseburensis

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3588 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G9MIK5|A0A1G9MIK5_9FIRM Dipeptidase putative OS=Romboutsia lituseburensis DSM 797 OX=1121325 GN=SAMN04515677_103220 PE=4 SV=1
MM1 pKa = 7.45NISFRR6 pKa = 11.84SSKK9 pKa = 9.98KK10 pKa = 10.32CNGKK14 pKa = 9.72RR15 pKa = 11.84SKK17 pKa = 10.62ILDD20 pKa = 4.48LINFNDD26 pKa = 4.4LLSDD30 pKa = 4.12SDD32 pKa = 3.63ILLYY36 pKa = 10.92DD37 pKa = 4.76DD38 pKa = 6.23DD39 pKa = 4.97YY40 pKa = 11.79DD41 pKa = 4.25YY42 pKa = 11.83DD43 pKa = 3.61NTYY46 pKa = 11.4DD47 pKa = 3.69EE48 pKa = 6.33DD49 pKa = 4.44YY50 pKa = 11.26DD51 pKa = 3.81SDD53 pKa = 4.33YY54 pKa = 11.48VISEE58 pKa = 4.99DD59 pKa = 3.7NLKK62 pKa = 9.74MEE64 pKa = 5.36DD65 pKa = 4.64DD66 pKa = 4.21VYY68 pKa = 11.56KK69 pKa = 10.9DD70 pKa = 3.19DD71 pKa = 4.37TIYY74 pKa = 11.31YY75 pKa = 9.74LL76 pKa = 4.16

Molecular weight: 9.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G9UHE2|A0A1G9UHE2_9FIRM 50S ribosomal protein L36 OS=Romboutsia lituseburensis DSM 797 OX=1121325 GN=rpmJ PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.67QPKK8 pKa = 9.0KK9 pKa = 7.87RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 9.77RR21 pKa = 11.84MKK23 pKa = 9.11TSNGRR28 pKa = 11.84NVLRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.7GRR39 pKa = 11.84NRR41 pKa = 11.84LTHH44 pKa = 6.18

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3588

0

3588

1087853

27

2692

303.2

34.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.316 ± 0.046

1.208 ± 0.019

5.738 ± 0.035

7.131 ± 0.046

4.23 ± 0.034

6.207 ± 0.041

1.347 ± 0.015

10.302 ± 0.059

9.235 ± 0.045

9.03 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.673 ± 0.021

6.78 ± 0.048

2.656 ± 0.02

2.479 ± 0.017

2.898 ± 0.024

6.463 ± 0.036

4.899 ± 0.033

6.539 ± 0.036

0.634 ± 0.014

4.234 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski