Streptomyces phage Kradal

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 325 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6E294|A0A4D6E294_9CAUD Uncharacterized protein OS=Streptomyces phage Kradal OX=2562187 GN=296 PE=4 SV=1
MM1 pKa = 8.23DD2 pKa = 3.7VLTWINTLVLGAVLVFGWIKK22 pKa = 10.35LANGDD27 pKa = 4.29EE28 pKa = 4.45ADD30 pKa = 3.87GCCAVILLVLTVGLAALGFLAYY52 pKa = 10.03GVGSLIGWWW61 pKa = 3.26

Molecular weight: 6.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6E225|A0A4D6E225_9CAUD Uncharacterized protein OS=Streptomyces phage Kradal OX=2562187 GN=226 PE=4 SV=1
MM1 pKa = 7.41MNTNPNDD8 pKa = 3.89PLVSIAEE15 pKa = 4.13AASRR19 pKa = 11.84MGVKK23 pKa = 10.24VGRR26 pKa = 11.84ARR28 pKa = 11.84SILAKK33 pKa = 10.54NLVRR37 pKa = 11.84PVRR40 pKa = 11.84TPAGIAYY47 pKa = 9.86RR48 pKa = 11.84LEE50 pKa = 4.25DD51 pKa = 3.53VVEE54 pKa = 4.55TKK56 pKa = 10.62RR57 pKa = 11.84MYY59 pKa = 10.12QQ60 pKa = 3.04

Molecular weight: 6.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

325

0

325

54557

29

2111

167.9

18.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.197 ± 0.312

1.111 ± 0.081

6.395 ± 0.126

7.128 ± 0.26

2.88 ± 0.079

8.886 ± 0.271

2.179 ± 0.102

4.08 ± 0.098

4.087 ± 0.156

8.146 ± 0.144

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.656 ± 0.101

2.863 ± 0.128

5.46 ± 0.144

3.202 ± 0.135

7.015 ± 0.242

5.708 ± 0.17

6.076 ± 0.174

7.412 ± 0.13

1.941 ± 0.086

2.577 ± 0.085
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski