Amino acid dipepetide frequency for Streptomyces phage Kradal

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.079AlaAla: 12.079 ± 1.076
0.898AlaCys: 0.898 ± 0.14
6.727AlaAsp: 6.727 ± 0.336
7.625AlaGlu: 7.625 ± 0.487
2.933AlaPhe: 2.933 ± 0.214
7.955AlaGly: 7.955 ± 0.58
1.833AlaHis: 1.833 ± 0.166
4.014AlaIle: 4.014 ± 0.408
3.868AlaLys: 3.868 ± 0.316
9.312AlaLeu: 9.312 ± 0.413
2.878AlaMet: 2.878 ± 0.271
2.713AlaAsn: 2.713 ± 0.283
4.857AlaPro: 4.857 ± 0.299
3.978AlaGln: 3.978 ± 0.425
6.837AlaArg: 6.837 ± 0.305
5.774AlaSer: 5.774 ± 0.461
5.664AlaThr: 5.664 ± 0.549
7.424AlaVal: 7.424 ± 0.346
1.705AlaTrp: 1.705 ± 0.177
2.896AlaTyr: 2.896 ± 0.227
0.0AlaXaa: 0.0 ± 0.0
Cys
0.715CysAla: 0.715 ± 0.11
0.22CysCys: 0.22 ± 0.073
0.697CysAsp: 0.697 ± 0.125
0.788CysGlu: 0.788 ± 0.137
0.257CysPhe: 0.257 ± 0.073
1.558CysGly: 1.558 ± 0.193
0.22CysHis: 0.22 ± 0.063
0.293CysIle: 0.293 ± 0.079
0.477CysLys: 0.477 ± 0.109
0.916CysLeu: 0.916 ± 0.139
0.128CysMet: 0.128 ± 0.052
0.183CysAsn: 0.183 ± 0.067
0.99CysPro: 0.99 ± 0.16
0.293CysGln: 0.293 ± 0.069
1.1CysArg: 1.1 ± 0.126
0.623CysSer: 0.623 ± 0.115
0.568CysThr: 0.568 ± 0.093
0.587CysVal: 0.587 ± 0.113
0.22CysTrp: 0.22 ± 0.054
0.275CysTyr: 0.275 ± 0.077
0.0CysXaa: 0.0 ± 0.0
Asp
5.884AspAla: 5.884 ± 0.312
0.752AspCys: 0.752 ± 0.118
4.179AspAsp: 4.179 ± 0.373
5.096AspGlu: 5.096 ± 0.329
1.998AspPhe: 1.998 ± 0.197
6.819AspGly: 6.819 ± 0.529
1.668AspHis: 1.668 ± 0.187
2.603AspIle: 2.603 ± 0.205
1.925AspLys: 1.925 ± 0.213
5.462AspLeu: 5.462 ± 0.3
1.65AspMet: 1.65 ± 0.203
1.43AspAsn: 1.43 ± 0.143
4.106AspPro: 4.106 ± 0.304
2.071AspGln: 2.071 ± 0.172
4.637AspArg: 4.637 ± 0.352
2.969AspSer: 2.969 ± 0.278
2.951AspThr: 2.951 ± 0.255
4.674AspVal: 4.674 ± 0.244
1.686AspTrp: 1.686 ± 0.178
1.393AspTyr: 1.393 ± 0.171
0.0AspXaa: 0.0 ± 0.0
Glu
6.984GluAla: 6.984 ± 0.467
0.587GluCys: 0.587 ± 0.128
4.216GluAsp: 4.216 ± 0.338
5.811GluGlu: 5.811 ± 0.416
2.181GluPhe: 2.181 ± 0.199
5.756GluGly: 5.756 ± 0.325
1.833GluHis: 1.833 ± 0.182
3.446GluIle: 3.446 ± 0.291
3.409GluLys: 3.409 ± 0.286
5.847GluLeu: 5.847 ± 0.381
2.163GluMet: 2.163 ± 0.221
2.2GluAsn: 2.2 ± 0.209
3.189GluPro: 3.189 ± 0.353
2.548GluGln: 2.548 ± 0.207
5.224GluArg: 5.224 ± 0.415
3.629GluSer: 3.629 ± 0.251
4.124GluThr: 4.124 ± 0.254
4.656GluVal: 4.656 ± 0.286
1.375GluTrp: 1.375 ± 0.163
2.108GluTyr: 2.108 ± 0.215
0.0GluXaa: 0.0 ± 0.0
Phe
2.31PheAla: 2.31 ± 0.249
0.697PheCys: 0.697 ± 0.106
2.071PheAsp: 2.071 ± 0.192
2.2PheGlu: 2.2 ± 0.182
0.953PhePhe: 0.953 ± 0.149
2.438PheGly: 2.438 ± 0.209
0.623PheHis: 0.623 ± 0.108
1.1PheIle: 1.1 ± 0.15
0.916PheLys: 0.916 ± 0.139
2.53PheLeu: 2.53 ± 0.196
0.568PheMet: 0.568 ± 0.11
0.77PheAsn: 0.77 ± 0.142
1.668PhePro: 1.668 ± 0.17
1.026PheGln: 1.026 ± 0.148
1.851PheArg: 1.851 ± 0.188
1.925PheSer: 1.925 ± 0.183
2.126PheThr: 2.126 ± 0.226
1.76PheVal: 1.76 ± 0.194
0.568PheTrp: 0.568 ± 0.084
0.697PheTyr: 0.697 ± 0.116
0.0PheXaa: 0.0 ± 0.0
Gly
7.662GlyAla: 7.662 ± 0.651
1.008GlyCys: 1.008 ± 0.152
5.756GlyAsp: 5.756 ± 0.327
6.067GlyGlu: 6.067 ± 0.339
3.061GlyPhe: 3.061 ± 0.232
7.039GlyGly: 7.039 ± 0.498
2.09GlyHis: 2.09 ± 0.198
3.941GlyIle: 3.941 ± 0.235
3.923GlyLys: 3.923 ± 0.364
6.764GlyLeu: 6.764 ± 0.324
2.438GlyMet: 2.438 ± 0.185
2.493GlyAsn: 2.493 ± 0.297
4.766GlyPro: 4.766 ± 0.421
2.914GlyGln: 2.914 ± 0.215
5.792GlyArg: 5.792 ± 0.36
6.489GlySer: 6.489 ± 0.363
6.269GlyThr: 6.269 ± 0.455
5.994GlyVal: 5.994 ± 0.313
2.181GlyTrp: 2.181 ± 0.194
2.218GlyTyr: 2.218 ± 0.145
0.0GlyXaa: 0.0 ± 0.0
His
2.053HisAla: 2.053 ± 0.221
0.257HisCys: 0.257 ± 0.062
1.393HisAsp: 1.393 ± 0.164
1.631HisGlu: 1.631 ± 0.185
0.605HisPhe: 0.605 ± 0.097
2.2HisGly: 2.2 ± 0.211
0.477HisHis: 0.477 ± 0.095
0.77HisIle: 0.77 ± 0.129
0.623HisLys: 0.623 ± 0.11
2.053HisLeu: 2.053 ± 0.216
0.385HisMet: 0.385 ± 0.086
0.513HisAsn: 0.513 ± 0.082
1.595HisPro: 1.595 ± 0.176
0.715HisGln: 0.715 ± 0.111
1.521HisArg: 1.521 ± 0.181
0.953HisSer: 0.953 ± 0.139
1.338HisThr: 1.338 ± 0.177
1.613HisVal: 1.613 ± 0.179
0.348HisTrp: 0.348 ± 0.09
0.752HisTyr: 0.752 ± 0.113
0.0HisXaa: 0.0 ± 0.0
Ile
3.959IleAla: 3.959 ± 0.286
0.532IleCys: 0.532 ± 0.09
3.043IleAsp: 3.043 ± 0.273
2.951IleGlu: 2.951 ± 0.276
0.971IlePhe: 0.971 ± 0.129
3.244IleGly: 3.244 ± 0.269
0.843IleHis: 0.843 ± 0.134
0.971IleIle: 0.971 ± 0.137
1.54IleLys: 1.54 ± 0.194
3.409IleLeu: 3.409 ± 0.215
0.843IleMet: 0.843 ± 0.114
1.173IleAsn: 1.173 ± 0.174
2.768IlePro: 2.768 ± 0.262
1.228IleGln: 1.228 ± 0.114
3.373IleArg: 3.373 ± 0.24
2.786IleSer: 2.786 ± 0.259
3.079IleThr: 3.079 ± 0.212
2.731IleVal: 2.731 ± 0.232
0.532IleTrp: 0.532 ± 0.096
0.825IleTyr: 0.825 ± 0.124
0.0IleXaa: 0.0 ± 0.0
Lys
4.491LysAla: 4.491 ± 0.338
0.367LysCys: 0.367 ± 0.072
2.31LysAsp: 2.31 ± 0.201
2.273LysGlu: 2.273 ± 0.244
1.026LysPhe: 1.026 ± 0.123
3.703LysGly: 3.703 ± 0.35
0.898LysHis: 0.898 ± 0.139
1.998LysIle: 1.998 ± 0.19
2.346LysLys: 2.346 ± 0.281
3.739LysLeu: 3.739 ± 0.397
1.063LysMet: 1.063 ± 0.126
1.393LysAsn: 1.393 ± 0.187
2.181LysPro: 2.181 ± 0.209
1.338LysGln: 1.338 ± 0.167
2.53LysArg: 2.53 ± 0.255
1.961LysSer: 1.961 ± 0.27
2.438LysThr: 2.438 ± 0.245
3.024LysVal: 3.024 ± 0.251
0.843LysTrp: 0.843 ± 0.111
0.953LysTyr: 0.953 ± 0.141
0.0LysXaa: 0.0 ± 0.0
Leu
9.99LeuAla: 9.99 ± 0.6
0.916LeuCys: 0.916 ± 0.152
5.407LeuAsp: 5.407 ± 0.341
4.986LeuGlu: 4.986 ± 0.356
1.888LeuPhe: 1.888 ± 0.182
6.452LeuGly: 6.452 ± 0.378
1.778LeuHis: 1.778 ± 0.195
3.336LeuIle: 3.336 ± 0.232
3.978LeuLys: 3.978 ± 0.277
6.91LeuLeu: 6.91 ± 0.411
2.255LeuMet: 2.255 ± 0.205
2.585LeuAsn: 2.585 ± 0.209
4.454LeuPro: 4.454 ± 0.334
1.668LeuGln: 1.668 ± 0.171
5.756LeuArg: 5.756 ± 0.34
4.399LeuSer: 4.399 ± 0.311
5.536LeuThr: 5.536 ± 0.279
5.792LeuVal: 5.792 ± 0.312
1.155LeuTrp: 1.155 ± 0.152
2.218LeuTyr: 2.218 ± 0.192
0.0LeuXaa: 0.0 ± 0.0
Met
3.556MetAla: 3.556 ± 0.284
0.128MetCys: 0.128 ± 0.049
1.888MetAsp: 1.888 ± 0.187
1.521MetGlu: 1.521 ± 0.183
0.532MetPhe: 0.532 ± 0.102
1.833MetGly: 1.833 ± 0.181
0.55MetHis: 0.55 ± 0.104
1.008MetIle: 1.008 ± 0.136
1.521MetLys: 1.521 ± 0.187
1.796MetLeu: 1.796 ± 0.198
0.568MetMet: 0.568 ± 0.104
1.283MetAsn: 1.283 ± 0.144
1.191MetPro: 1.191 ± 0.152
0.971MetGln: 0.971 ± 0.143
1.888MetArg: 1.888 ± 0.209
1.87MetSer: 1.87 ± 0.204
1.961MetThr: 1.961 ± 0.192
1.466MetVal: 1.466 ± 0.138
0.513MetTrp: 0.513 ± 0.098
0.513MetTyr: 0.513 ± 0.119
0.0MetXaa: 0.0 ± 0.0
Asn
3.244AsnAla: 3.244 ± 0.314
0.238AsnCys: 0.238 ± 0.071
1.576AsnAsp: 1.576 ± 0.148
1.815AsnGlu: 1.815 ± 0.178
0.605AsnPhe: 0.605 ± 0.141
3.721AsnGly: 3.721 ± 0.378
0.568AsnHis: 0.568 ± 0.111
0.971AsnIle: 0.971 ± 0.127
1.008AsnLys: 1.008 ± 0.145
2.383AsnLeu: 2.383 ± 0.258
0.623AsnMet: 0.623 ± 0.112
0.825AsnAsn: 0.825 ± 0.132
2.291AsnPro: 2.291 ± 0.192
0.916AsnGln: 0.916 ± 0.145
1.851AsnArg: 1.851 ± 0.155
1.503AsnSer: 1.503 ± 0.186
1.54AsnThr: 1.54 ± 0.236
1.851AsnVal: 1.851 ± 0.178
0.385AsnTrp: 0.385 ± 0.084
0.715AsnTyr: 0.715 ± 0.111
0.0AsnXaa: 0.0 ± 0.0
Pro
4.894ProAla: 4.894 ± 0.322
0.825ProCys: 0.825 ± 0.14
3.648ProAsp: 3.648 ± 0.258
5.517ProGlu: 5.517 ± 0.407
1.411ProPhe: 1.411 ± 0.178
5.976ProGly: 5.976 ± 0.41
1.173ProHis: 1.173 ± 0.15
1.32ProIle: 1.32 ± 0.152
2.09ProLys: 2.09 ± 0.255
3.611ProLeu: 3.611 ± 0.272
1.43ProMet: 1.43 ± 0.148
1.246ProAsn: 1.246 ± 0.14
2.951ProPro: 2.951 ± 0.27
2.016ProGln: 2.016 ± 0.204
3.263ProArg: 3.263 ± 0.286
3.134ProSer: 3.134 ± 0.271
2.841ProThr: 2.841 ± 0.248
4.931ProVal: 4.931 ± 0.312
0.953ProTrp: 0.953 ± 0.129
1.375ProTyr: 1.375 ± 0.143
0.0ProXaa: 0.0 ± 0.0
Gln
3.666GlnAla: 3.666 ± 0.353
0.44GlnCys: 0.44 ± 0.087
1.815GlnAsp: 1.815 ± 0.163
2.053GlnGlu: 2.053 ± 0.194
1.026GlnPhe: 1.026 ± 0.129
2.841GlnGly: 2.841 ± 0.215
0.807GlnHis: 0.807 ± 0.123
1.906GlnIle: 1.906 ± 0.183
1.411GlnLys: 1.411 ± 0.257
2.09GlnLeu: 2.09 ± 0.208
0.825GlnMet: 0.825 ± 0.121
0.99GlnAsn: 0.99 ± 0.159
1.466GlnPro: 1.466 ± 0.164
1.375GlnGln: 1.375 ± 0.216
2.236GlnArg: 2.236 ± 0.211
1.521GlnSer: 1.521 ± 0.206
1.43GlnThr: 1.43 ± 0.162
2.438GlnVal: 2.438 ± 0.237
0.587GlnTrp: 0.587 ± 0.102
1.1GlnTyr: 1.1 ± 0.124
0.0GlnXaa: 0.0 ± 0.0
Arg
6.819ArgAla: 6.819 ± 0.421
0.697ArgCys: 0.697 ± 0.124
4.564ArgAsp: 4.564 ± 0.276
5.756ArgGlu: 5.756 ± 0.446
2.2ArgPhe: 2.2 ± 0.208
4.821ArgGly: 4.821 ± 0.333
1.686ArgHis: 1.686 ± 0.191
3.868ArgIle: 3.868 ± 0.246
3.318ArgLys: 3.318 ± 0.266
5.554ArgLeu: 5.554 ± 0.338
2.236ArgMet: 2.236 ± 0.249
2.346ArgAsn: 2.346 ± 0.205
3.116ArgPro: 3.116 ± 0.307
2.09ArgGln: 2.09 ± 0.21
5.536ArgArg: 5.536 ± 0.387
3.556ArgSer: 3.556 ± 0.285
3.941ArgThr: 3.941 ± 0.324
4.821ArgVal: 4.821 ± 0.344
1.54ArgTrp: 1.54 ± 0.167
1.686ArgTyr: 1.686 ± 0.193
0.0ArgXaa: 0.0 ± 0.0
Ser
5.902SerAla: 5.902 ± 0.524
0.642SerCys: 0.642 ± 0.113
3.629SerAsp: 3.629 ± 0.244
3.428SerGlu: 3.428 ± 0.261
1.796SerPhe: 1.796 ± 0.195
6.709SerGly: 6.709 ± 0.359
0.935SerHis: 0.935 ± 0.13
2.145SerIle: 2.145 ± 0.178
2.291SerLys: 2.291 ± 0.266
4.381SerLeu: 4.381 ± 0.29
1.888SerMet: 1.888 ± 0.202
1.228SerAsn: 1.228 ± 0.188
2.749SerPro: 2.749 ± 0.216
1.43SerGln: 1.43 ± 0.141
4.051SerArg: 4.051 ± 0.319
3.354SerSer: 3.354 ± 0.303
3.153SerThr: 3.153 ± 0.305
4.912SerVal: 4.912 ± 0.328
1.155SerTrp: 1.155 ± 0.146
1.283SerTyr: 1.283 ± 0.166
0.0SerXaa: 0.0 ± 0.0
Thr
6.58ThrAla: 6.58 ± 0.529
0.66ThrCys: 0.66 ± 0.13
3.593ThrAsp: 3.593 ± 0.277
3.666ThrGlu: 3.666 ± 0.267
1.705ThrPhe: 1.705 ± 0.183
6.58ThrGly: 6.58 ± 0.407
1.081ThrHis: 1.081 ± 0.139
2.328ThrIle: 2.328 ± 0.209
1.925ThrLys: 1.925 ± 0.229
4.546ThrLeu: 4.546 ± 0.309
1.393ThrMet: 1.393 ± 0.159
1.723ThrAsn: 1.723 ± 0.222
3.721ThrPro: 3.721 ± 0.28
1.833ThrGln: 1.833 ± 0.239
3.648ThrArg: 3.648 ± 0.237
3.538ThrSer: 3.538 ± 0.296
3.483ThrThr: 3.483 ± 0.266
5.976ThrVal: 5.976 ± 0.329
1.228ThrTrp: 1.228 ± 0.155
1.558ThrTyr: 1.558 ± 0.185
0.0ThrXaa: 0.0 ± 0.0
Val
7.075ValAla: 7.075 ± 0.355
0.77ValCys: 0.77 ± 0.12
4.417ValAsp: 4.417 ± 0.333
5.224ValGlu: 5.224 ± 0.287
2.585ValPhe: 2.585 ± 0.203
5.114ValGly: 5.114 ± 0.424
1.851ValHis: 1.851 ± 0.189
3.226ValIle: 3.226 ± 0.257
2.713ValLys: 2.713 ± 0.23
5.646ValLeu: 5.646 ± 0.288
2.181ValMet: 2.181 ± 0.18
2.2ValAsn: 2.2 ± 0.194
4.124ValPro: 4.124 ± 0.288
2.438ValGln: 2.438 ± 0.234
5.627ValArg: 5.627 ± 0.314
4.491ValSer: 4.491 ± 0.282
5.316ValThr: 5.316 ± 0.315
5.646ValVal: 5.646 ± 0.337
1.411ValTrp: 1.411 ± 0.156
2.071ValTyr: 2.071 ± 0.174
0.0ValXaa: 0.0 ± 0.0
Trp
1.338TrpAla: 1.338 ± 0.148
0.275TrpCys: 0.275 ± 0.07
1.338TrpAsp: 1.338 ± 0.15
1.136TrpGlu: 1.136 ± 0.154
0.477TrpPhe: 0.477 ± 0.086
1.301TrpGly: 1.301 ± 0.128
0.367TrpHis: 0.367 ± 0.082
0.898TrpIle: 0.898 ± 0.131
0.843TrpLys: 0.843 ± 0.122
2.035TrpLeu: 2.035 ± 0.205
0.587TrpMet: 0.587 ± 0.101
0.568TrpAsn: 0.568 ± 0.114
0.99TrpPro: 0.99 ± 0.131
0.513TrpGln: 0.513 ± 0.093
1.283TrpArg: 1.283 ± 0.169
1.301TrpSer: 1.301 ± 0.149
1.558TrpThr: 1.558 ± 0.202
1.54TrpVal: 1.54 ± 0.158
0.495TrpTrp: 0.495 ± 0.11
0.568TrpTyr: 0.568 ± 0.092
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.768TyrAla: 2.768 ± 0.217
0.202TyrCys: 0.202 ± 0.068
1.686TyrAsp: 1.686 ± 0.202
1.741TyrGlu: 1.741 ± 0.179
0.587TyrPhe: 0.587 ± 0.099
2.804TyrGly: 2.804 ± 0.287
0.513TyrHis: 0.513 ± 0.106
0.66TyrIle: 0.66 ± 0.132
0.752TyrLys: 0.752 ± 0.131
2.475TyrLeu: 2.475 ± 0.193
0.44TyrMet: 0.44 ± 0.084
0.77TyrAsn: 0.77 ± 0.138
1.43TyrPro: 1.43 ± 0.15
0.66TyrGln: 0.66 ± 0.093
2.145TyrArg: 2.145 ± 0.178
1.301TyrSer: 1.301 ± 0.172
1.448TyrThr: 1.448 ± 0.168
2.291TyrVal: 2.291 ± 0.239
0.532TyrTrp: 0.532 ± 0.099
0.568TyrTyr: 0.568 ± 0.123
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 325 proteins (54557 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski