Pelagibacter phage HTVC109P

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Pelagivirus; unclassified Pelagivirus

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y1NWR1|A0A4Y1NWR1_9CAUD Lysozyme OS=Pelagibacter phage HTVC109P OX=2283019 GN=P109_gp15 PE=4 SV=1
MM1 pKa = 7.41SLVIYY6 pKa = 8.77QTDD9 pKa = 3.74EE10 pKa = 3.72LRR12 pKa = 11.84KK13 pKa = 8.27QTKK16 pKa = 8.92QKK18 pKa = 9.98QEE20 pKa = 3.61KK21 pKa = 8.62TMDD24 pKa = 3.45YY25 pKa = 10.49QKK27 pKa = 11.05EE28 pKa = 4.2YY29 pKa = 11.26NDD31 pKa = 4.08WLDD34 pKa = 3.5EE35 pKa = 3.95LHH37 pKa = 6.73PLEE40 pKa = 6.35GIACNCFSTLLEE52 pKa = 4.62AGDD55 pKa = 4.62PIAYY59 pKa = 9.77NCGYY63 pKa = 11.09DD64 pKa = 5.08DD65 pKa = 5.27FLDD68 pKa = 3.89ANGYY72 pKa = 8.21EE73 pKa = 4.39EE74 pKa = 4.27EE75 pKa = 4.26

Molecular weight: 8.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y1NWQ3|A0A4Y1NWQ3_9CAUD Endodeoxyribonuclease I OS=Pelagibacter phage HTVC109P OX=2283019 GN=P109_gp14 PE=4 SV=1
MM1 pKa = 7.72KK2 pKa = 10.34SRR4 pKa = 11.84NRR6 pKa = 11.84AEE8 pKa = 3.88NAALSVALLMTRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84TLFFKK27 pKa = 10.94VPIIVTRR34 pKa = 11.84RR35 pKa = 11.84QHH37 pKa = 5.55KK38 pKa = 8.19QNKK41 pKa = 8.37GAITMIDD48 pKa = 3.55TIKK51 pKa = 10.64IIGTVIGAGVFVFMAWAMIWVACAMSDD78 pKa = 3.66QCWYY82 pKa = 11.04NYY84 pKa = 9.14TGQLL88 pKa = 3.44

Molecular weight: 10.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

12950

39

1268

239.8

26.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.069 ± 0.509

0.919 ± 0.166

6.085 ± 0.249

6.409 ± 0.468

3.761 ± 0.212

6.432 ± 0.386

1.807 ± 0.24

6.378 ± 0.184

8.062 ± 0.516

8.185 ± 0.423

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.641 ± 0.219

5.923 ± 0.466

3.066 ± 0.198

3.915 ± 0.277

4.023 ± 0.271

6.394 ± 0.471

7.181 ± 0.617

5.869 ± 0.33

1.274 ± 0.122

3.606 ± 0.194
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski