Amino acid dipepetide frequency for Pelagibacter phage HTVC109P

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.788AlaAla: 4.788 ± 0.862
0.618AlaCys: 0.618 ± 0.238
6.333AlaAsp: 6.333 ± 0.946
4.711AlaGlu: 4.711 ± 0.721
3.089AlaPhe: 3.089 ± 0.457
6.178AlaGly: 6.178 ± 1.408
1.313AlaHis: 1.313 ± 0.319
4.711AlaIle: 4.711 ± 0.71
6.333AlaLys: 6.333 ± 0.964
6.333AlaLeu: 6.333 ± 0.685
2.857AlaMet: 2.857 ± 0.421
5.792AlaAsn: 5.792 ± 1.264
2.317AlaPro: 2.317 ± 0.446
3.166AlaGln: 3.166 ± 0.462
2.626AlaArg: 2.626 ± 0.643
5.174AlaSer: 5.174 ± 0.745
4.788AlaThr: 4.788 ± 0.931
4.711AlaVal: 4.711 ± 0.949
1.313AlaTrp: 1.313 ± 0.295
3.552AlaTyr: 3.552 ± 0.547
0.0AlaXaa: 0.0 ± 0.0
Cys
0.541CysAla: 0.541 ± 0.194
0.154CysCys: 0.154 ± 0.1
0.463CysAsp: 0.463 ± 0.204
0.541CysGlu: 0.541 ± 0.165
0.618CysPhe: 0.618 ± 0.253
0.541CysGly: 0.541 ± 0.231
0.154CysHis: 0.154 ± 0.113
0.772CysIle: 0.772 ± 0.264
0.463CysLys: 0.463 ± 0.198
1.158CysLeu: 1.158 ± 0.334
0.154CysMet: 0.154 ± 0.11
0.695CysAsn: 0.695 ± 0.26
0.077CysPro: 0.077 ± 0.084
0.463CysGln: 0.463 ± 0.19
0.695CysArg: 0.695 ± 0.215
0.618CysSer: 0.618 ± 0.239
0.309CysThr: 0.309 ± 0.155
0.232CysVal: 0.232 ± 0.13
0.309CysTrp: 0.309 ± 0.163
0.232CysTyr: 0.232 ± 0.163
0.0CysXaa: 0.0 ± 0.0
Asp
4.325AspAla: 4.325 ± 0.594
0.309AspCys: 0.309 ± 0.167
3.861AspAsp: 3.861 ± 0.644
3.63AspGlu: 3.63 ± 0.683
2.857AspPhe: 2.857 ± 0.511
3.63AspGly: 3.63 ± 0.643
0.386AspHis: 0.386 ± 0.159
4.17AspIle: 4.17 ± 0.425
4.479AspLys: 4.479 ± 0.598
5.638AspLeu: 5.638 ± 0.649
1.236AspMet: 1.236 ± 0.338
2.935AspAsn: 2.935 ± 0.535
2.317AspPro: 2.317 ± 0.656
1.313AspGln: 1.313 ± 0.375
3.089AspArg: 3.089 ± 0.618
3.475AspSer: 3.475 ± 0.609
5.097AspThr: 5.097 ± 0.972
4.17AspVal: 4.17 ± 0.649
0.849AspTrp: 0.849 ± 0.315
3.089AspTyr: 3.089 ± 0.431
0.0AspXaa: 0.0 ± 0.0
Glu
5.56GluAla: 5.56 ± 0.829
0.772GluCys: 0.772 ± 0.285
3.089GluAsp: 3.089 ± 0.48
4.942GluGlu: 4.942 ± 0.627
2.24GluPhe: 2.24 ± 0.462
3.475GluGly: 3.475 ± 0.686
0.849GluHis: 0.849 ± 0.251
4.17GluIle: 4.17 ± 0.468
5.483GluLys: 5.483 ± 0.672
6.333GluLeu: 6.333 ± 0.668
2.008GluMet: 2.008 ± 0.422
3.012GluAsn: 3.012 ± 0.443
1.313GluPro: 1.313 ± 0.435
2.857GluGln: 2.857 ± 0.51
3.63GluArg: 3.63 ± 0.699
3.012GluSer: 3.012 ± 0.594
4.788GluThr: 4.788 ± 0.71
3.089GluVal: 3.089 ± 0.404
0.927GluTrp: 0.927 ± 0.335
2.548GluTyr: 2.548 ± 0.364
0.0GluXaa: 0.0 ± 0.0
Phe
1.622PheAla: 1.622 ± 0.352
0.463PheCys: 0.463 ± 0.178
2.394PheAsp: 2.394 ± 0.439
2.626PheGlu: 2.626 ± 0.605
1.776PhePhe: 1.776 ± 0.425
2.548PheGly: 2.548 ± 0.602
0.618PheHis: 0.618 ± 0.175
2.703PheIle: 2.703 ± 0.428
3.398PheLys: 3.398 ± 0.574
3.475PheLeu: 3.475 ± 0.666
1.931PheMet: 1.931 ± 0.249
2.085PheAsn: 2.085 ± 0.416
0.772PhePro: 0.772 ± 0.253
1.545PheGln: 1.545 ± 0.317
1.081PheArg: 1.081 ± 0.314
2.162PheSer: 2.162 ± 0.296
2.857PheThr: 2.857 ± 0.543
2.008PheVal: 2.008 ± 0.398
0.309PheTrp: 0.309 ± 0.146
1.236PheTyr: 1.236 ± 0.351
0.0PheXaa: 0.0 ± 0.0
Gly
4.711GlyAla: 4.711 ± 0.725
0.309GlyCys: 0.309 ± 0.185
3.861GlyAsp: 3.861 ± 0.386
2.935GlyGlu: 2.935 ± 0.583
2.703GlyPhe: 2.703 ± 0.398
4.402GlyGly: 4.402 ± 0.728
0.927GlyHis: 0.927 ± 0.302
3.939GlyIle: 3.939 ± 0.49
5.097GlyLys: 5.097 ± 0.852
5.097GlyLeu: 5.097 ± 0.634
1.467GlyMet: 1.467 ± 0.303
3.861GlyAsn: 3.861 ± 0.617
0.077GlyPro: 0.077 ± 0.081
2.935GlyGln: 2.935 ± 0.55
2.085GlyArg: 2.085 ± 0.452
5.56GlySer: 5.56 ± 1.139
5.329GlyThr: 5.329 ± 0.926
4.788GlyVal: 4.788 ± 0.395
1.236GlyTrp: 1.236 ± 0.339
2.935GlyTyr: 2.935 ± 0.55
0.0GlyXaa: 0.0 ± 0.0
His
1.622HisAla: 1.622 ± 0.286
0.463HisCys: 0.463 ± 0.194
0.695HisAsp: 0.695 ± 0.266
0.772HisGlu: 0.772 ± 0.219
0.541HisPhe: 0.541 ± 0.199
1.004HisGly: 1.004 ± 0.275
0.386HisHis: 0.386 ± 0.202
1.699HisIle: 1.699 ± 0.436
1.081HisLys: 1.081 ± 0.363
1.699HisLeu: 1.699 ± 0.429
0.618HisMet: 0.618 ± 0.197
0.772HisAsn: 0.772 ± 0.229
0.618HisPro: 0.618 ± 0.193
0.695HisGln: 0.695 ± 0.215
0.541HisArg: 0.541 ± 0.209
1.313HisSer: 1.313 ± 0.348
1.313HisThr: 1.313 ± 0.258
1.081HisVal: 1.081 ± 0.266
0.618HisTrp: 0.618 ± 0.207
0.541HisTyr: 0.541 ± 0.213
0.0HisXaa: 0.0 ± 0.0
Ile
6.255IleAla: 6.255 ± 0.723
0.618IleCys: 0.618 ± 0.22
4.247IleAsp: 4.247 ± 0.498
4.634IleGlu: 4.634 ± 0.674
1.699IlePhe: 1.699 ± 0.381
4.788IleGly: 4.788 ± 0.529
1.467IleHis: 1.467 ± 0.387
4.17IleIle: 4.17 ± 0.645
5.946IleLys: 5.946 ± 0.632
3.861IleLeu: 3.861 ± 0.535
1.236IleMet: 1.236 ± 0.299
3.707IleAsn: 3.707 ± 0.685
2.857IlePro: 2.857 ± 0.575
2.008IleGln: 2.008 ± 0.402
2.085IleArg: 2.085 ± 0.369
3.475IleSer: 3.475 ± 0.656
4.711IleThr: 4.711 ± 0.478
3.243IleVal: 3.243 ± 0.526
0.618IleTrp: 0.618 ± 0.222
2.162IleTyr: 2.162 ± 0.477
0.0IleXaa: 0.0 ± 0.0
Lys
5.946LysAla: 5.946 ± 0.955
0.618LysCys: 0.618 ± 0.245
5.097LysAsp: 5.097 ± 0.464
6.41LysGlu: 6.41 ± 0.916
2.703LysPhe: 2.703 ± 0.456
4.788LysGly: 4.788 ± 0.726
1.39LysHis: 1.39 ± 0.343
5.406LysIle: 5.406 ± 0.602
6.255LysLys: 6.255 ± 1.077
7.568LysLeu: 7.568 ± 0.696
1.467LysMet: 1.467 ± 0.406
4.093LysAsn: 4.093 ± 0.644
3.475LysPro: 3.475 ± 0.54
3.63LysGln: 3.63 ± 0.692
4.479LysArg: 4.479 ± 0.919
3.475LysSer: 3.475 ± 0.717
5.56LysThr: 5.56 ± 0.651
5.02LysVal: 5.02 ± 0.798
0.618LysTrp: 0.618 ± 0.267
2.626LysTyr: 2.626 ± 0.53
0.0LysXaa: 0.0 ± 0.0
Leu
6.41LeuAla: 6.41 ± 0.564
0.541LeuCys: 0.541 ± 0.19
5.638LeuAsp: 5.638 ± 0.636
5.792LeuGlu: 5.792 ± 0.747
2.471LeuPhe: 2.471 ± 0.436
4.016LeuGly: 4.016 ± 0.509
1.853LeuHis: 1.853 ± 0.394
4.325LeuIle: 4.325 ± 0.702
7.105LeuLys: 7.105 ± 0.792
6.796LeuLeu: 6.796 ± 0.934
2.008LeuMet: 2.008 ± 0.406
5.251LeuAsn: 5.251 ± 0.594
3.398LeuPro: 3.398 ± 0.446
4.325LeuGln: 4.325 ± 0.649
3.939LeuArg: 3.939 ± 0.683
5.097LeuSer: 5.097 ± 0.694
5.406LeuThr: 5.406 ± 0.618
4.402LeuVal: 4.402 ± 0.661
1.081LeuTrp: 1.081 ± 0.311
2.008LeuTyr: 2.008 ± 0.423
0.0LeuXaa: 0.0 ± 0.0
Met
2.548MetAla: 2.548 ± 0.453
0.463MetCys: 0.463 ± 0.186
1.236MetAsp: 1.236 ± 0.355
1.313MetGlu: 1.313 ± 0.298
0.849MetPhe: 0.849 ± 0.224
1.931MetGly: 1.931 ± 0.398
0.386MetHis: 0.386 ± 0.137
2.317MetIle: 2.317 ± 0.457
2.317MetLys: 2.317 ± 0.502
2.394MetLeu: 2.394 ± 0.518
0.695MetMet: 0.695 ± 0.183
0.849MetAsn: 0.849 ± 0.381
1.39MetPro: 1.39 ± 0.354
0.695MetGln: 0.695 ± 0.216
1.236MetArg: 1.236 ± 0.281
1.853MetSer: 1.853 ± 0.401
1.776MetThr: 1.776 ± 0.319
1.467MetVal: 1.467 ± 0.371
0.309MetTrp: 0.309 ± 0.171
0.386MetTyr: 0.386 ± 0.167
0.0MetXaa: 0.0 ± 0.0
Asn
6.024AsnAla: 6.024 ± 0.918
0.618AsnCys: 0.618 ± 0.269
3.166AsnAsp: 3.166 ± 0.471
2.935AsnGlu: 2.935 ± 0.407
2.394AsnPhe: 2.394 ± 0.478
4.093AsnGly: 4.093 ± 0.631
0.772AsnHis: 0.772 ± 0.227
4.016AsnIle: 4.016 ± 0.786
4.17AsnLys: 4.17 ± 0.687
4.788AsnLeu: 4.788 ± 0.511
1.467AsnMet: 1.467 ± 0.36
3.63AsnAsn: 3.63 ± 0.642
2.162AsnPro: 2.162 ± 0.395
1.931AsnGln: 1.931 ± 0.334
2.703AsnArg: 2.703 ± 0.443
3.552AsnSer: 3.552 ± 0.957
4.325AsnThr: 4.325 ± 0.747
3.552AsnVal: 3.552 ± 0.91
0.386AsnTrp: 0.386 ± 0.203
2.548AsnTyr: 2.548 ± 0.372
0.0AsnXaa: 0.0 ± 0.0
Pro
1.931ProAla: 1.931 ± 0.362
0.154ProCys: 0.154 ± 0.099
1.853ProAsp: 1.853 ± 0.384
3.243ProGlu: 3.243 ± 0.623
1.853ProPhe: 1.853 ± 0.358
0.0ProGly: 0.0 ± 0.0
0.695ProHis: 0.695 ± 0.225
1.622ProIle: 1.622 ± 0.31
2.857ProLys: 2.857 ± 0.555
2.471ProLeu: 2.471 ± 0.386
0.618ProMet: 0.618 ± 0.223
2.162ProAsn: 2.162 ± 0.474
0.849ProPro: 0.849 ± 0.284
1.236ProGln: 1.236 ± 0.255
0.463ProArg: 0.463 ± 0.171
2.703ProSer: 2.703 ± 0.388
2.626ProThr: 2.626 ± 0.437
2.162ProVal: 2.162 ± 0.396
0.0ProTrp: 0.0 ± 0.0
1.158ProTyr: 1.158 ± 0.337
0.0ProXaa: 0.0 ± 0.0
Gln
3.012GlnAla: 3.012 ± 0.594
0.154GlnCys: 0.154 ± 0.124
2.085GlnAsp: 2.085 ± 0.401
2.394GlnGlu: 2.394 ± 0.505
1.313GlnPhe: 1.313 ± 0.326
1.931GlnGly: 1.931 ± 0.359
0.695GlnHis: 0.695 ± 0.272
3.243GlnIle: 3.243 ± 0.484
3.243GlnLys: 3.243 ± 0.615
3.321GlnLeu: 3.321 ± 0.617
1.545GlnMet: 1.545 ± 0.4
1.853GlnAsn: 1.853 ± 0.4
0.849GlnPro: 0.849 ± 0.234
1.467GlnGln: 1.467 ± 0.476
1.776GlnArg: 1.776 ± 0.34
2.935GlnSer: 2.935 ± 0.604
2.703GlnThr: 2.703 ± 0.426
2.626GlnVal: 2.626 ± 0.398
0.695GlnTrp: 0.695 ± 0.171
1.313GlnTyr: 1.313 ± 0.369
0.0GlnXaa: 0.0 ± 0.0
Arg
2.626ArgAla: 2.626 ± 0.525
0.154ArgCys: 0.154 ± 0.102
2.78ArgAsp: 2.78 ± 0.368
3.089ArgGlu: 3.089 ± 0.732
1.931ArgPhe: 1.931 ± 0.332
2.548ArgGly: 2.548 ± 0.463
1.313ArgHis: 1.313 ± 0.354
2.008ArgIle: 2.008 ± 0.4
2.78ArgLys: 2.78 ± 0.637
3.166ArgLeu: 3.166 ± 0.606
1.622ArgMet: 1.622 ± 0.318
2.317ArgAsn: 2.317 ± 0.391
0.772ArgPro: 0.772 ± 0.194
1.622ArgGln: 1.622 ± 0.276
2.317ArgArg: 2.317 ± 0.488
2.24ArgSer: 2.24 ± 0.32
2.394ArgThr: 2.394 ± 0.417
2.085ArgVal: 2.085 ± 0.425
0.463ArgTrp: 0.463 ± 0.188
2.008ArgTyr: 2.008 ± 0.309
0.0ArgXaa: 0.0 ± 0.0
Ser
4.865SerAla: 4.865 ± 0.654
0.695SerCys: 0.695 ± 0.283
4.17SerAsp: 4.17 ± 0.641
3.552SerGlu: 3.552 ± 0.487
2.78SerPhe: 2.78 ± 0.406
5.792SerGly: 5.792 ± 0.954
1.158SerHis: 1.158 ± 0.262
4.093SerIle: 4.093 ± 0.619
5.02SerLys: 5.02 ± 0.672
4.016SerLeu: 4.016 ± 0.602
1.39SerMet: 1.39 ± 0.409
4.479SerAsn: 4.479 ± 1.087
2.008SerPro: 2.008 ± 0.393
2.162SerGln: 2.162 ± 0.51
1.853SerArg: 1.853 ± 0.36
4.711SerSer: 4.711 ± 0.896
4.247SerThr: 4.247 ± 0.89
3.707SerVal: 3.707 ± 0.519
0.927SerTrp: 0.927 ± 0.254
2.317SerTyr: 2.317 ± 0.459
0.0SerXaa: 0.0 ± 0.0
Thr
8.032ThrAla: 8.032 ± 1.136
0.618ThrCys: 0.618 ± 0.217
3.243ThrAsp: 3.243 ± 0.554
4.402ThrGlu: 4.402 ± 0.777
2.162ThrPhe: 2.162 ± 0.502
5.56ThrGly: 5.56 ± 0.799
1.853ThrHis: 1.853 ± 0.354
4.556ThrIle: 4.556 ± 0.531
5.869ThrLys: 5.869 ± 0.907
5.638ThrLeu: 5.638 ± 0.833
1.699ThrMet: 1.699 ± 0.333
4.479ThrAsn: 4.479 ± 0.999
2.703ThrPro: 2.703 ± 0.373
2.548ThrGln: 2.548 ± 0.504
1.853ThrArg: 1.853 ± 0.422
3.861ThrSer: 3.861 ± 0.737
5.638ThrThr: 5.638 ± 1.3
4.479ThrVal: 4.479 ± 1.168
0.541ThrTrp: 0.541 ± 0.161
2.085ThrTyr: 2.085 ± 0.36
0.0ThrXaa: 0.0 ± 0.0
Val
6.564ValAla: 6.564 ± 1.214
0.849ValCys: 0.849 ± 0.272
3.243ValAsp: 3.243 ± 0.483
4.17ValGlu: 4.17 ± 0.613
2.085ValPhe: 2.085 ± 0.305
3.861ValGly: 3.861 ± 0.602
1.004ValHis: 1.004 ± 0.306
3.398ValIle: 3.398 ± 0.565
4.402ValLys: 4.402 ± 0.526
3.552ValLeu: 3.552 ± 0.626
1.467ValMet: 1.467 ± 0.419
3.939ValAsn: 3.939 ± 0.787
1.776ValPro: 1.776 ± 0.393
1.776ValGln: 1.776 ± 0.274
2.162ValArg: 2.162 ± 0.525
4.788ValSer: 4.788 ± 0.622
4.402ValThr: 4.402 ± 0.643
3.166ValVal: 3.166 ± 0.44
0.618ValTrp: 0.618 ± 0.199
1.467ValTyr: 1.467 ± 0.307
0.0ValXaa: 0.0 ± 0.0
Trp
0.695TrpAla: 0.695 ± 0.285
0.077TrpCys: 0.077 ± 0.085
1.158TrpAsp: 1.158 ± 0.303
0.309TrpGlu: 0.309 ± 0.143
0.386TrpPhe: 0.386 ± 0.189
0.541TrpGly: 0.541 ± 0.252
0.077TrpHis: 0.077 ± 0.075
0.772TrpIle: 0.772 ± 0.234
1.004TrpLys: 1.004 ± 0.29
1.931TrpLeu: 1.931 ± 0.356
0.0TrpMet: 0.0 ± 0.0
1.004TrpAsn: 1.004 ± 0.252
0.0TrpPro: 0.0 ± 0.0
0.849TrpGln: 0.849 ± 0.223
0.386TrpArg: 0.386 ± 0.134
1.081TrpSer: 1.081 ± 0.288
1.158TrpThr: 1.158 ± 0.347
0.772TrpVal: 0.772 ± 0.257
0.154TrpTrp: 0.154 ± 0.17
0.386TrpTyr: 0.386 ± 0.246
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.626TyrAla: 2.626 ± 0.519
0.541TyrCys: 0.541 ± 0.227
2.24TyrAsp: 2.24 ± 0.417
1.699TyrGlu: 1.699 ± 0.378
1.158TyrPhe: 1.158 ± 0.325
2.703TyrGly: 2.703 ± 0.392
0.772TyrHis: 0.772 ± 0.243
1.699TyrIle: 1.699 ± 0.339
3.321TyrLys: 3.321 ± 0.653
2.626TyrLeu: 2.626 ± 0.459
0.849TyrMet: 0.849 ± 0.254
2.317TyrAsn: 2.317 ± 0.412
0.927TyrPro: 0.927 ± 0.258
1.931TyrGln: 1.931 ± 0.444
1.236TyrArg: 1.236 ± 0.356
2.857TyrSer: 2.857 ± 0.36
2.394TyrThr: 2.394 ± 0.557
1.931TyrVal: 1.931 ± 0.525
0.772TyrTrp: 0.772 ± 0.217
1.467TyrTyr: 1.467 ± 0.447
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (12950 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski