Lactobacillus amylolyticus DSM 11664

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lactobacillus; Lactobacillus amylolyticus

Average proteome isoelectric point is 7.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1684 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4YUI2|D4YUI2_9LACO Uncharacterized protein OS=Lactobacillus amylolyticus DSM 11664 OX=585524 GN=HMPREF0493_1193 PE=4 SV=1
MM1 pKa = 7.84DD2 pKa = 4.84AVFNTVFSGDD12 pKa = 3.14QTMIPSSIKK21 pKa = 9.88IYY23 pKa = 9.94EE24 pKa = 4.41VPSDD28 pKa = 3.73MAVDD32 pKa = 4.24DD33 pKa = 4.2SGDD36 pKa = 3.19RR37 pKa = 11.84HH38 pKa = 6.96NEE40 pKa = 3.37DD41 pKa = 4.17DD42 pKa = 4.55QYY44 pKa = 11.47IDD46 pKa = 4.36PSDD49 pKa = 3.66PTQQATYY56 pKa = 9.5YY57 pKa = 10.64NKK59 pKa = 8.58ITAGTPDD66 pKa = 3.59TAFKK70 pKa = 10.88NYY72 pKa = 10.18LLAHH76 pKa = 5.23VTYY79 pKa = 10.73DD80 pKa = 4.24EE81 pKa = 4.25NGKK84 pKa = 9.59PNGFAVDD91 pKa = 3.71QSSDD95 pKa = 3.17GLFNIDD101 pKa = 4.02GNTDD105 pKa = 3.84YY106 pKa = 11.43ASHH109 pKa = 7.18AYY111 pKa = 9.58FIQVDD116 pKa = 3.9TVMNDD121 pKa = 3.24PNKK124 pKa = 10.78APGDD128 pKa = 3.65GTTITTHH135 pKa = 6.4TSQTLNGTGITLDD148 pKa = 4.09DD149 pKa = 3.92TEE151 pKa = 4.53SWTGAVTGNGGGQNQTEE168 pKa = 4.2AAHH171 pKa = 6.12VRR173 pKa = 11.84FF174 pKa = 4.48

Molecular weight: 18.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4YSI2|D4YSI2_9LACO FAD_binding_2 domain-containing protein OS=Lactobacillus amylolyticus DSM 11664 OX=585524 GN=HMPREF0493_0460 PE=4 SV=1
MM1 pKa = 7.87PKK3 pKa = 9.59MKK5 pKa = 8.8THH7 pKa = 6.78RR8 pKa = 11.84ASAKK12 pKa = 9.47RR13 pKa = 11.84FKK15 pKa = 10.03RR16 pKa = 11.84TANGGLKK23 pKa = 9.5RR24 pKa = 11.84HH25 pKa = 6.15HH26 pKa = 7.23AFTGHH31 pKa = 6.77RR32 pKa = 11.84FHH34 pKa = 7.54GKK36 pKa = 6.24TKK38 pKa = 9.47KK39 pKa = 9.37QRR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.45LRR45 pKa = 11.84KK46 pKa = 8.75AAMVSRR52 pKa = 11.84SDD54 pKa = 3.45MKK56 pKa = 10.58RR57 pKa = 11.84IKK59 pKa = 10.66QMLSQMRR66 pKa = 3.59

Molecular weight: 7.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1684

0

1684

457019

39

1435

271.4

30.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.388 ± 0.056

0.608 ± 0.015

5.99 ± 0.064

5.675 ± 0.057

4.38 ± 0.053

6.338 ± 0.061

2.167 ± 0.029

7.484 ± 0.063

8.108 ± 0.067

9.504 ± 0.074

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.725 ± 0.03

5.288 ± 0.05

3.444 ± 0.035

4.082 ± 0.039

3.992 ± 0.046

5.88 ± 0.052

5.517 ± 0.044

6.639 ± 0.054

0.995 ± 0.022

3.796 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski