Amino acid dipepetide frequency for Lactobacillus amylolyticus DSM 11664

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.192AlaAla: 6.192 ± 0.156
0.562AlaCys: 0.562 ± 0.036
4.343AlaAsp: 4.343 ± 0.115
4.12AlaGlu: 4.12 ± 0.117
3.096AlaPhe: 3.096 ± 0.087
5.118AlaGly: 5.118 ± 0.104
1.427AlaHis: 1.427 ± 0.051
5.836AlaIle: 5.836 ± 0.135
6.486AlaLys: 6.486 ± 0.123
6.855AlaLeu: 6.855 ± 0.134
2.0AlaMet: 2.0 ± 0.069
3.529AlaAsn: 3.529 ± 0.094
1.93AlaPro: 1.93 ± 0.063
3.063AlaGln: 3.063 ± 0.087
2.81AlaArg: 2.81 ± 0.086
4.409AlaSer: 4.409 ± 0.11
3.987AlaThr: 3.987 ± 0.098
4.949AlaVal: 4.949 ± 0.101
0.586AlaTrp: 0.586 ± 0.036
2.58AlaTyr: 2.58 ± 0.075
0.0AlaXaa: 0.0 ± 0.0
Cys
0.424CysAla: 0.424 ± 0.028
0.077CysCys: 0.077 ± 0.014
0.403CysAsp: 0.403 ± 0.033
0.271CysGlu: 0.271 ± 0.021
0.322CysPhe: 0.322 ± 0.026
0.538CysGly: 0.538 ± 0.034
0.188CysHis: 0.188 ± 0.023
0.389CysIle: 0.389 ± 0.031
0.258CysLys: 0.258 ± 0.02
0.687CysLeu: 0.687 ± 0.042
0.168CysMet: 0.168 ± 0.02
0.256CysAsn: 0.256 ± 0.024
0.254CysPro: 0.254 ± 0.026
0.245CysGln: 0.245 ± 0.027
0.214CysArg: 0.214 ± 0.021
0.446CysSer: 0.446 ± 0.034
0.254CysThr: 0.254 ± 0.026
0.341CysVal: 0.341 ± 0.028
0.085CysTrp: 0.085 ± 0.016
0.258CysTyr: 0.258 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
3.987AspAla: 3.987 ± 0.1
0.326AspCys: 0.326 ± 0.026
3.65AspAsp: 3.65 ± 0.123
4.4AspGlu: 4.4 ± 0.108
2.974AspPhe: 2.974 ± 0.093
3.626AspGly: 3.626 ± 0.1
1.532AspHis: 1.532 ± 0.06
3.746AspIle: 3.746 ± 0.096
4.483AspLys: 4.483 ± 0.099
6.094AspLeu: 6.094 ± 0.132
1.435AspMet: 1.435 ± 0.063
2.748AspAsn: 2.748 ± 0.077
2.595AspPro: 2.595 ± 0.082
3.109AspGln: 3.109 ± 0.096
2.343AspArg: 2.343 ± 0.084
3.081AspSer: 3.081 ± 0.09
2.661AspThr: 2.661 ± 0.086
3.685AspVal: 3.685 ± 0.1
0.726AspTrp: 0.726 ± 0.042
2.702AspTyr: 2.702 ± 0.077
0.0AspXaa: 0.0 ± 0.0
Glu
4.258GluAla: 4.258 ± 0.103
0.278GluCys: 0.278 ± 0.027
3.392GluAsp: 3.392 ± 0.102
3.621GluGlu: 3.621 ± 0.095
2.164GluPhe: 2.164 ± 0.069
2.866GluGly: 2.866 ± 0.086
1.173GluHis: 1.173 ± 0.054
4.567GluIle: 4.567 ± 0.113
5.831GluLys: 5.831 ± 0.131
5.459GluLeu: 5.459 ± 0.149
1.803GluMet: 1.803 ± 0.064
3.376GluAsn: 3.376 ± 0.091
1.569GluPro: 1.569 ± 0.059
2.387GluGln: 2.387 ± 0.08
2.368GluArg: 2.368 ± 0.073
2.529GluSer: 2.529 ± 0.076
2.81GluThr: 2.81 ± 0.08
3.829GluVal: 3.829 ± 0.1
0.479GluTrp: 0.479 ± 0.037
1.993GluTyr: 1.993 ± 0.07
0.0GluXaa: 0.0 ± 0.0
Phe
3.346PheAla: 3.346 ± 0.083
0.333PheCys: 0.333 ± 0.029
2.899PheAsp: 2.899 ± 0.082
2.203PheGlu: 2.203 ± 0.074
2.219PhePhe: 2.219 ± 0.076
3.09PheGly: 3.09 ± 0.086
0.985PheHis: 0.985 ± 0.049
3.162PheIle: 3.162 ± 0.102
3.039PheLys: 3.039 ± 0.09
4.192PheLeu: 4.192 ± 0.123
1.177PheMet: 1.177 ± 0.056
2.394PheAsn: 2.394 ± 0.07
1.49PhePro: 1.49 ± 0.064
1.14PheGln: 1.14 ± 0.052
1.418PheArg: 1.418 ± 0.057
2.947PheSer: 2.947 ± 0.081
2.429PheThr: 2.429 ± 0.082
3.024PheVal: 3.024 ± 0.088
0.58PheTrp: 0.58 ± 0.03
1.737PheTyr: 1.737 ± 0.07
0.0PheXaa: 0.0 ± 0.0
Gly
4.227GlyAla: 4.227 ± 0.115
0.403GlyCys: 0.403 ± 0.028
3.431GlyAsp: 3.431 ± 0.106
3.381GlyGlu: 3.381 ± 0.094
2.86GlyPhe: 2.86 ± 0.082
3.958GlyGly: 3.958 ± 0.116
1.51GlyHis: 1.51 ± 0.064
5.529GlyIle: 5.529 ± 0.13
5.547GlyLys: 5.547 ± 0.101
6.131GlyLeu: 6.131 ± 0.149
1.81GlyMet: 1.81 ± 0.059
2.899GlyAsn: 2.899 ± 0.078
1.459GlyPro: 1.459 ± 0.056
2.606GlyGln: 2.606 ± 0.083
2.637GlyArg: 2.637 ± 0.08
3.862GlySer: 3.862 ± 0.109
3.672GlyThr: 3.672 ± 0.093
4.114GlyVal: 4.114 ± 0.099
0.661GlyTrp: 0.661 ± 0.041
2.68GlyTyr: 2.68 ± 0.075
0.0GlyXaa: 0.0 ± 0.0
His
1.435HisAla: 1.435 ± 0.052
0.158HisCys: 0.158 ± 0.019
1.403HisAsp: 1.403 ± 0.055
1.287HisGlu: 1.287 ± 0.051
1.151HisPhe: 1.151 ± 0.049
1.575HisGly: 1.575 ± 0.068
0.726HisHis: 0.726 ± 0.04
1.249HisIle: 1.249 ± 0.054
1.354HisLys: 1.354 ± 0.053
2.059HisLeu: 2.059 ± 0.06
0.554HisMet: 0.554 ± 0.031
0.989HisAsn: 0.989 ± 0.047
1.055HisPro: 1.055 ± 0.051
0.895HisGln: 0.895 ± 0.046
0.945HisArg: 0.945 ± 0.047
1.096HisSer: 1.096 ± 0.053
0.989HisThr: 0.989 ± 0.055
1.44HisVal: 1.44 ± 0.061
0.241HisTrp: 0.241 ± 0.023
1.066HisTyr: 1.066 ± 0.049
0.0HisXaa: 0.0 ± 0.0
Ile
6.251IleAla: 6.251 ± 0.133
0.619IleCys: 0.619 ± 0.038
4.879IleAsp: 4.879 ± 0.114
3.796IleGlu: 3.796 ± 0.088
3.376IlePhe: 3.376 ± 0.097
5.055IleGly: 5.055 ± 0.124
1.35IleHis: 1.35 ± 0.058
5.617IleIle: 5.617 ± 0.135
5.628IleLys: 5.628 ± 0.116
6.724IleLeu: 6.724 ± 0.14
1.98IleMet: 1.98 ± 0.067
4.065IleAsn: 4.065 ± 0.089
2.895IlePro: 2.895 ± 0.076
2.488IleGln: 2.488 ± 0.088
2.755IleArg: 2.755 ± 0.083
4.785IleSer: 4.785 ± 0.093
3.991IleThr: 3.991 ± 0.11
5.361IleVal: 5.361 ± 0.129
0.746IleTrp: 0.746 ± 0.044
2.477IleTyr: 2.477 ± 0.084
0.0IleXaa: 0.0 ± 0.0
Lys
5.479LysAla: 5.479 ± 0.126
0.269LysCys: 0.269 ± 0.024
4.871LysAsp: 4.871 ± 0.117
5.28LysGlu: 5.28 ± 0.114
2.639LysPhe: 2.639 ± 0.079
4.009LysGly: 4.009 ± 0.094
1.659LysHis: 1.659 ± 0.065
6.265LysIle: 6.265 ± 0.116
8.313LysLys: 8.313 ± 0.168
7.219LysLeu: 7.219 ± 0.14
2.775LysMet: 2.775 ± 0.079
4.982LysAsn: 4.982 ± 0.102
2.433LysPro: 2.433 ± 0.073
3.704LysGln: 3.704 ± 0.116
3.739LysArg: 3.739 ± 0.106
3.893LysSer: 3.893 ± 0.086
4.676LysThr: 4.676 ± 0.118
4.844LysVal: 4.844 ± 0.105
0.705LysTrp: 0.705 ± 0.037
3.33LysTyr: 3.33 ± 0.092
0.0LysXaa: 0.0 ± 0.0
Leu
7.809LeuAla: 7.809 ± 0.154
0.573LeuCys: 0.573 ± 0.043
5.505LeuAsp: 5.505 ± 0.136
4.514LeuGlu: 4.514 ± 0.108
4.096LeuPhe: 4.096 ± 0.103
6.004LeuGly: 6.004 ± 0.13
1.996LeuHis: 1.996 ± 0.064
7.293LeuIle: 7.293 ± 0.159
7.542LeuLys: 7.542 ± 0.146
8.949LeuLeu: 8.949 ± 0.198
2.387LeuMet: 2.387 ± 0.076
5.219LeuAsn: 5.219 ± 0.1
3.823LeuPro: 3.823 ± 0.093
3.521LeuGln: 3.521 ± 0.125
3.674LeuArg: 3.674 ± 0.092
6.216LeuSer: 6.216 ± 0.112
6.114LeuThr: 6.114 ± 0.111
6.155LeuVal: 6.155 ± 0.12
0.829LeuTrp: 0.829 ± 0.049
2.825LeuTyr: 2.825 ± 0.074
0.0LeuXaa: 0.0 ± 0.0
Met
2.265MetAla: 2.265 ± 0.073
0.147MetCys: 0.147 ± 0.018
1.438MetAsp: 1.438 ± 0.059
1.214MetGlu: 1.214 ± 0.056
0.967MetPhe: 0.967 ± 0.045
1.606MetGly: 1.606 ± 0.054
0.617MetHis: 0.617 ± 0.035
2.168MetIle: 2.168 ± 0.073
2.381MetLys: 2.381 ± 0.084
2.372MetLeu: 2.372 ± 0.076
0.891MetMet: 0.891 ± 0.039
1.58MetAsn: 1.58 ± 0.062
1.267MetPro: 1.267 ± 0.051
1.206MetGln: 1.206 ± 0.048
1.158MetArg: 1.158 ± 0.046
1.54MetSer: 1.54 ± 0.056
1.807MetThr: 1.807 ± 0.058
1.715MetVal: 1.715 ± 0.058
0.208MetTrp: 0.208 ± 0.021
0.705MetTyr: 0.705 ± 0.039
0.0MetXaa: 0.0 ± 0.0
Asn
3.433AsnAla: 3.433 ± 0.088
0.394AsnCys: 0.394 ± 0.027
3.16AsnAsp: 3.16 ± 0.1
2.901AsnGlu: 2.901 ± 0.072
2.33AsnPhe: 2.33 ± 0.062
3.803AsnGly: 3.803 ± 0.096
1.182AsnHis: 1.182 ± 0.053
3.306AsnIle: 3.306 ± 0.095
4.21AsnLys: 4.21 ± 0.103
5.12AsnLeu: 5.12 ± 0.112
1.328AsnMet: 1.328 ± 0.055
3.059AsnAsn: 3.059 ± 0.091
2.214AsnPro: 2.214 ± 0.067
2.368AsnGln: 2.368 ± 0.074
2.033AsnArg: 2.033 ± 0.075
3.157AsnSer: 3.157 ± 0.094
2.543AsnThr: 2.543 ± 0.077
3.223AsnVal: 3.223 ± 0.086
0.705AsnTrp: 0.705 ± 0.041
2.405AsnTyr: 2.405 ± 0.081
0.0AsnXaa: 0.0 ± 0.0
Pro
2.446ProAla: 2.446 ± 0.077
0.149ProCys: 0.149 ± 0.019
2.464ProAsp: 2.464 ± 0.075
2.827ProGlu: 2.827 ± 0.079
1.591ProPhe: 1.591 ± 0.052
2.12ProGly: 2.12 ± 0.062
0.683ProHis: 0.683 ± 0.043
2.464ProIle: 2.464 ± 0.078
2.669ProLys: 2.669 ± 0.078
2.989ProLeu: 2.989 ± 0.083
0.792ProMet: 0.792 ± 0.044
1.993ProAsn: 1.993 ± 0.067
0.523ProPro: 0.523 ± 0.039
1.333ProGln: 1.333 ± 0.056
1.14ProArg: 1.14 ± 0.049
1.967ProSer: 1.967 ± 0.073
2.024ProThr: 2.024 ± 0.076
2.582ProVal: 2.582 ± 0.08
0.348ProTrp: 0.348 ± 0.029
1.339ProTyr: 1.339 ± 0.053
0.0ProXaa: 0.0 ± 0.0
Gln
3.096GlnAla: 3.096 ± 0.092
0.12GlnCys: 0.12 ± 0.018
2.236GlnAsp: 2.236 ± 0.081
2.514GlnGlu: 2.514 ± 0.069
1.501GlnPhe: 1.501 ± 0.059
2.055GlnGly: 2.055 ± 0.066
0.772GlnHis: 0.772 ± 0.042
3.497GlnIle: 3.497 ± 0.094
3.713GlnLys: 3.713 ± 0.098
4.114GlnLeu: 4.114 ± 0.116
1.328GlnMet: 1.328 ± 0.064
2.262GlnAsn: 2.262 ± 0.063
1.241GlnPro: 1.241 ± 0.049
1.663GlnGln: 1.663 ± 0.069
1.705GlnArg: 1.705 ± 0.053
1.991GlnSer: 1.991 ± 0.065
2.33GlnThr: 2.33 ± 0.081
2.838GlnVal: 2.838 ± 0.071
0.357GlnTrp: 0.357 ± 0.026
1.486GlnTyr: 1.486 ± 0.062
0.0GlnXaa: 0.0 ± 0.0
Arg
2.521ArgAla: 2.521 ± 0.081
0.269ArgCys: 0.269 ± 0.023
2.201ArgAsp: 2.201 ± 0.069
2.508ArgGlu: 2.508 ± 0.073
1.779ArgPhe: 1.779 ± 0.061
2.144ArgGly: 2.144 ± 0.082
0.978ArgHis: 0.978 ± 0.045
3.258ArgIle: 3.258 ± 0.082
3.418ArgLys: 3.418 ± 0.085
3.731ArgLeu: 3.731 ± 0.101
1.179ArgMet: 1.179 ± 0.049
2.024ArgAsn: 2.024 ± 0.061
1.416ArgPro: 1.416 ± 0.058
1.779ArgGln: 1.779 ± 0.053
2.147ArgArg: 2.147 ± 0.08
2.101ArgSer: 2.101 ± 0.065
1.904ArgThr: 1.904 ± 0.064
2.595ArgVal: 2.595 ± 0.084
0.344ArgTrp: 0.344 ± 0.027
1.624ArgTyr: 1.624 ± 0.063
0.0ArgXaa: 0.0 ± 0.0
Ser
3.934SerAla: 3.934 ± 0.112
0.306SerCys: 0.306 ± 0.025
3.508SerAsp: 3.508 ± 0.098
3.136SerGlu: 3.136 ± 0.083
2.976SerPhe: 2.976 ± 0.09
4.308SerGly: 4.308 ± 0.11
1.193SerHis: 1.193 ± 0.05
3.934SerIle: 3.934 ± 0.105
4.276SerLys: 4.276 ± 0.114
5.781SerLeu: 5.781 ± 0.114
1.462SerMet: 1.462 ± 0.057
2.985SerAsn: 2.985 ± 0.09
1.731SerPro: 1.731 ± 0.057
2.387SerGln: 2.387 ± 0.083
2.258SerArg: 2.258 ± 0.077
4.444SerSer: 4.444 ± 0.171
3.317SerThr: 3.317 ± 0.099
3.674SerVal: 3.674 ± 0.089
0.698SerTrp: 0.698 ± 0.044
2.497SerTyr: 2.497 ± 0.072
0.0SerXaa: 0.0 ± 0.0
Thr
4.422ThrAla: 4.422 ± 0.103
0.28ThrCys: 0.28 ± 0.027
3.269ThrAsp: 3.269 ± 0.086
2.871ThrGlu: 2.871 ± 0.089
2.378ThrPhe: 2.378 ± 0.074
3.954ThrGly: 3.954 ± 0.101
1.037ThrHis: 1.037 ± 0.047
4.402ThrIle: 4.402 ± 0.11
4.098ThrLys: 4.098 ± 0.105
4.963ThrLeu: 4.963 ± 0.111
1.35ThrMet: 1.35 ± 0.05
2.617ThrAsn: 2.617 ± 0.082
2.372ThrPro: 2.372 ± 0.073
2.017ThrGln: 2.017 ± 0.069
1.912ThrArg: 1.912 ± 0.062
3.295ThrSer: 3.295 ± 0.108
3.398ThrThr: 3.398 ± 0.112
4.125ThrVal: 4.125 ± 0.103
0.514ThrTrp: 0.514 ± 0.028
1.891ThrTyr: 1.891 ± 0.076
0.0ThrXaa: 0.0 ± 0.0
Val
5.258ValAla: 5.258 ± 0.111
0.411ValCys: 0.411 ± 0.031
3.904ValAsp: 3.904 ± 0.095
3.56ValGlu: 3.56 ± 0.102
2.807ValPhe: 2.807 ± 0.078
4.457ValGly: 4.457 ± 0.117
1.26ValHis: 1.26 ± 0.06
5.131ValIle: 5.131 ± 0.118
5.26ValLys: 5.26 ± 0.102
6.186ValLeu: 6.186 ± 0.116
1.781ValMet: 1.781 ± 0.064
3.37ValAsn: 3.37 ± 0.085
2.464ValPro: 2.464 ± 0.069
2.352ValGln: 2.352 ± 0.077
2.335ValArg: 2.335 ± 0.073
4.149ValSer: 4.149 ± 0.1
3.947ValThr: 3.947 ± 0.096
4.888ValVal: 4.888 ± 0.114
0.527ValTrp: 0.527 ± 0.033
2.341ValTyr: 2.341 ± 0.067
0.0ValXaa: 0.0 ± 0.0
Trp
0.645TrpAla: 0.645 ± 0.038
0.077TrpCys: 0.077 ± 0.013
0.516TrpAsp: 0.516 ± 0.031
0.337TrpGlu: 0.337 ± 0.029
0.508TrpPhe: 0.508 ± 0.031
0.632TrpGly: 0.632 ± 0.041
0.319TrpHis: 0.319 ± 0.029
0.731TrpIle: 0.731 ± 0.046
0.556TrpLys: 0.556 ± 0.04
1.249TrpLeu: 1.249 ± 0.059
0.284TrpMet: 0.284 ± 0.024
0.584TrpAsn: 0.584 ± 0.034
0.284TrpPro: 0.284 ± 0.028
0.58TrpGln: 0.58 ± 0.036
0.416TrpArg: 0.416 ± 0.031
0.543TrpSer: 0.543 ± 0.038
0.501TrpThr: 0.501 ± 0.037
0.63TrpVal: 0.63 ± 0.04
0.14TrpTrp: 0.14 ± 0.02
0.418TrpTyr: 0.418 ± 0.028
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.448TyrAla: 2.448 ± 0.072
0.33TyrCys: 0.33 ± 0.024
2.433TyrAsp: 2.433 ± 0.074
2.011TyrGlu: 2.011 ± 0.07
2.07TyrPhe: 2.07 ± 0.076
2.457TyrGly: 2.457 ± 0.063
1.081TyrHis: 1.081 ± 0.048
2.295TyrIle: 2.295 ± 0.069
2.02TyrLys: 2.02 ± 0.074
4.171TyrLeu: 4.171 ± 0.11
0.764TyrMet: 0.764 ± 0.041
1.943TyrAsn: 1.943 ± 0.067
1.429TyrPro: 1.429 ± 0.058
1.976TyrGln: 1.976 ± 0.071
1.912TyrArg: 1.912 ± 0.067
2.35TyrSer: 2.35 ± 0.076
1.814TyrThr: 1.814 ± 0.058
2.376TyrVal: 2.376 ± 0.078
0.473TyrTrp: 0.473 ± 0.036
1.606TyrTyr: 1.606 ± 0.063
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1684 proteins (457019 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski