Lucilia cuprina (Green bottle fly) (Australian sheep blowfly)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha;

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14353 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L0BL23|A0A0L0BL23_LUCCU CULLIN_2 domain-containing protein OS=Lucilia cuprina OX=7375 GN=FF38_07904 PE=3 SV=1
MM1 pKa = 7.73SDD3 pKa = 3.23VDD5 pKa = 5.09DD6 pKa = 5.52DD7 pKa = 5.77FNFLNCQWDD16 pKa = 3.72VMIITSGAEE25 pKa = 3.62FLMSINTFDD34 pKa = 4.53SSTLVCLPLPPEE46 pKa = 4.45SPVLACSLCRR56 pKa = 11.84PLEE59 pKa = 4.12PVEE62 pKa = 4.17IAVTTAVPGAGICVTLGGICDD83 pKa = 4.16DD84 pKa = 4.66DD85 pKa = 4.95AVVACVDD92 pKa = 3.65DD93 pKa = 4.2AVVVVVDD100 pKa = 4.2DD101 pKa = 4.54GVAVFAFVVAVVNVADD117 pKa = 5.56AGDD120 pKa = 4.25DD121 pKa = 3.56DD122 pKa = 5.65DD123 pKa = 5.14RR124 pKa = 11.84FPVRR128 pKa = 11.84VYY130 pKa = 10.95DD131 pKa = 3.78VNADD135 pKa = 3.56VAVDD139 pKa = 3.52VAVDD143 pKa = 3.64VVVDD147 pKa = 3.66VALASSSLDD156 pKa = 3.23SCGRR160 pKa = 11.84CILRR164 pKa = 11.84LDD166 pKa = 3.85NVDD169 pKa = 3.47TVGGGGGAICAASYY183 pKa = 10.24PKK185 pKa = 10.32VEE187 pKa = 4.09KK188 pKa = 10.55RR189 pKa = 11.84GGGGDD194 pKa = 3.92TIDD197 pKa = 3.21MLSRR201 pKa = 11.84EE202 pKa = 4.26RR203 pKa = 11.84AFEE206 pKa = 3.88LRR208 pKa = 11.84PISSNLDD215 pKa = 3.48SLDD218 pKa = 3.2WQYY221 pKa = 12.0VKK223 pKa = 10.84LVAGLGNSFEE233 pKa = 4.49LYY235 pKa = 10.18LYY237 pKa = 10.85NFLEE241 pKa = 4.75SKK243 pKa = 10.29PQDD246 pKa = 3.24TSAHH250 pKa = 5.87HH251 pKa = 6.7LCFFF255 pKa = 5.08

Molecular weight: 26.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L0CSZ1|A0A0L0CSZ1_LUCCU SUEL-type lectin domain-containing protein (Fragment) OS=Lucilia cuprina OX=7375 GN=FF38_02861 PE=4 SV=1
MM1 pKa = 7.21MSSTLSKK8 pKa = 10.35IHH10 pKa = 5.38VTNSTGLSLNEE21 pKa = 3.83RR22 pKa = 11.84FTAISTQPKK31 pKa = 8.77VVTNIGATRR40 pKa = 11.84RR41 pKa = 11.84SRR43 pKa = 11.84SRR45 pKa = 11.84SRR47 pKa = 11.84SVQRR51 pKa = 11.84QVPVAAAAEE60 pKa = 4.73RR61 pKa = 11.84ITSRR65 pKa = 11.84ANQRR69 pKa = 11.84LLAQLQRR76 pKa = 11.84KK77 pKa = 8.82HH78 pKa = 6.1KK79 pKa = 9.9VQAALKK85 pKa = 9.19LKK87 pKa = 9.02RR88 pKa = 11.84RR89 pKa = 11.84SMRR92 pKa = 11.84TVGGRR97 pKa = 11.84SNGSGGANGRR107 pKa = 11.84IRR109 pKa = 11.84RR110 pKa = 11.84GTVKK114 pKa = 10.58AFRR117 pKa = 11.84VGANGKK123 pKa = 8.74PIRR126 pKa = 11.84TNSLTRR132 pKa = 11.84IATMNADD139 pKa = 4.4RR140 pKa = 11.84EE141 pKa = 4.48ITNNRR146 pKa = 11.84SRR148 pKa = 11.84PLRR151 pKa = 11.84RR152 pKa = 11.84SNSSNNLAGRR162 pKa = 11.84LGPRR166 pKa = 11.84RR167 pKa = 11.84PTVGGAAQRR176 pKa = 11.84VARR179 pKa = 11.84RR180 pKa = 11.84NIDD183 pKa = 3.38RR184 pKa = 11.84QRR186 pKa = 11.84GRR188 pKa = 11.84NNVGIRR194 pKa = 11.84GRR196 pKa = 11.84STGINNRR203 pKa = 11.84GRR205 pKa = 11.84SRR207 pKa = 11.84SRR209 pKa = 11.84SRR211 pKa = 11.84TRR213 pKa = 11.84TLSDD217 pKa = 2.75NGRR220 pKa = 11.84NRR222 pKa = 11.84SRR224 pKa = 11.84TRR226 pKa = 11.84NNTDD230 pKa = 2.65NQLNNRR236 pKa = 11.84SRR238 pKa = 11.84SRR240 pKa = 11.84NRR242 pKa = 11.84NSANIQTNNNNNNVRR257 pKa = 11.84RR258 pKa = 11.84GRR260 pKa = 11.84SRR262 pKa = 11.84SRR264 pKa = 11.84SRR266 pKa = 11.84GRR268 pKa = 11.84AAVGSVNKK276 pKa = 10.54SNLPIKK282 pKa = 10.41ARR284 pKa = 11.84LGVRR288 pKa = 11.84PGVTANRR295 pKa = 11.84GTNNNRR301 pKa = 11.84RR302 pKa = 11.84GRR304 pKa = 11.84NASLTRR310 pKa = 11.84WGVQTGRR317 pKa = 11.84VQKK320 pKa = 10.39RR321 pKa = 11.84RR322 pKa = 11.84NSTNVNAAGNDD333 pKa = 3.42NNGRR337 pKa = 11.84GRR339 pKa = 11.84MRR341 pKa = 11.84SRR343 pKa = 11.84SAVRR347 pKa = 11.84NNSNNGNVRR356 pKa = 11.84SRR358 pKa = 11.84SRR360 pKa = 11.84SRR362 pKa = 11.84TRR364 pKa = 11.84KK365 pKa = 9.23GQVGQQRR372 pKa = 11.84QQQGSGQVRR381 pKa = 11.84QQRR384 pKa = 11.84NNRR387 pKa = 11.84APRR390 pKa = 11.84GRR392 pKa = 11.84QQQRR396 pKa = 11.84RR397 pKa = 11.84GNNTGNGQRR406 pKa = 11.84GTNNNGKK413 pKa = 9.07AQTQQRR419 pKa = 11.84RR420 pKa = 11.84GRR422 pKa = 11.84SRR424 pKa = 11.84SRR426 pKa = 11.84TGRR429 pKa = 11.84GANSGGNSRR438 pKa = 11.84NQGNKK443 pKa = 9.04SAAATKK449 pKa = 9.88PAVNKK454 pKa = 10.21DD455 pKa = 3.63ALDD458 pKa = 4.25KK459 pKa = 11.39EE460 pKa = 4.61LDD462 pKa = 3.34QYY464 pKa = 11.17MATTKK469 pKa = 10.12TEE471 pKa = 3.65HH472 pKa = 6.1EE473 pKa = 3.96MDD475 pKa = 3.8YY476 pKa = 11.27LLKK479 pKa = 10.9NN480 pKa = 3.63

Molecular weight: 53.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14353

0

14353

7420241

30

19217

517.0

58.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.249 ± 0.024

1.914 ± 0.028

5.18 ± 0.021

6.492 ± 0.04

3.761 ± 0.02

5.069 ± 0.026

2.604 ± 0.013

5.815 ± 0.021

6.594 ± 0.032

8.867 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.3 ± 0.012

6.209 ± 0.028

4.87 ± 0.028

4.941 ± 0.03

4.607 ± 0.019

8.344 ± 0.035

6.51 ± 0.03

5.545 ± 0.022

0.909 ± 0.008

3.212 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski