Amino acid dipepetide frequency for Lucilia cuprina (Green bottle fly) (Australian sheep blowfly)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.788AlaAla: 6.788 ± 0.093	1.034AlaCys: 1.034 ± 0.022	2.859AlaAsp: 2.859 ± 0.024	3.799AlaGlu: 3.799 ± 0.039	2.129AlaPhe: 2.129 ± 0.021	3.45AlaGly: 3.45 ± 0.043	1.349AlaHis: 1.349 ± 0.017	3.495AlaIle: 3.495 ± 0.027	3.863AlaLys: 3.863 ± 0.031	5.417AlaLeu: 5.417 ± 0.038	1.393AlaMet: 1.393 ± 0.017	3.54AlaAsn: 3.54 ± 0.026	2.986AlaPro: 2.986 ± 0.055	2.563AlaGln: 2.563 ± 0.028	2.413AlaArg: 2.413 ± 0.023	4.933AlaSer: 4.933 ± 0.04	4.484AlaThr: 4.484 ± 0.038	3.873AlaVal: 3.873 ± 0.033	0.464AlaTrp: 0.464 ± 0.01	1.658AlaTyr: 1.658 ± 0.024	0.0AlaXaa: 0.0 ± 0.0
Cys	1.017CysAla: 1.017 ± 0.017	0.523CysCys: 0.523 ± 0.011	1.082CysAsp: 1.082 ± 0.022	1.158CysGlu: 1.158 ± 0.02	0.772CysPhe: 0.772 ± 0.012	1.252CysGly: 1.252 ± 0.033	0.48CysHis: 0.48 ± 0.011	1.129CysIle: 1.129 ± 0.024	1.141CysLys: 1.141 ± 0.017	1.811CysLeu: 1.811 ± 0.026	0.402CysMet: 0.402 ± 0.008	1.101CysAsn: 1.101 ± 0.019	0.959CysPro: 0.959 ± 0.031	0.771CysGln: 0.771 ± 0.02	0.957CysArg: 0.957 ± 0.037	1.668CysSer: 1.668 ± 0.032	1.005CysThr: 1.005 ± 0.018	1.116CysVal: 1.116 ± 0.026	0.197CysTrp: 0.197 ± 0.005	0.597CysTyr: 0.597 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	2.74AspAla: 2.74 ± 0.026	1.009AspCys: 1.009 ± 0.018	4.195AspAsp: 4.195 ± 0.048	4.206AspGlu: 4.206 ± 0.042	2.333AspPhe: 2.333 ± 0.02	2.583AspGly: 2.583 ± 0.041	1.094AspHis: 1.094 ± 0.014	3.596AspIle: 3.596 ± 0.032	3.221AspLys: 3.221 ± 0.03	4.719AspLeu: 4.719 ± 0.033	1.231AspMet: 1.231 ± 0.014	3.198AspAsn: 3.198 ± 0.03	2.017AspPro: 2.017 ± 0.034	1.537AspGln: 1.537 ± 0.017	1.99AspArg: 1.99 ± 0.024	3.876AspSer: 3.876 ± 0.033	2.587AspThr: 2.587 ± 0.026	3.212AspVal: 3.212 ± 0.025	0.529AspTrp: 0.529 ± 0.009	1.925AspTyr: 1.925 ± 0.018	0.0AspXaa: 0.0 ± 0.0
Glu	3.641GluAla: 3.641 ± 0.036	1.195GluCys: 1.195 ± 0.033	4.012GluAsp: 4.012 ± 0.037	6.303GluGlu: 6.303 ± 0.103	2.343GluPhe: 2.343 ± 0.021	2.565GluGly: 2.565 ± 0.024	1.575GluHis: 1.575 ± 0.019	4.14GluIle: 4.14 ± 0.036	4.944GluLys: 4.944 ± 0.057	5.587GluLeu: 5.587 ± 0.04	1.615GluMet: 1.615 ± 0.017	4.142GluAsn: 4.142 ± 0.034	2.294GluPro: 2.294 ± 0.029	3.045GluGln: 3.045 ± 0.035	3.295GluArg: 3.295 ± 0.035	4.289GluSer: 4.289 ± 0.053	3.935GluThr: 3.935 ± 0.051	3.431GluVal: 3.431 ± 0.037	0.549GluTrp: 0.549 ± 0.011	2.018GluTyr: 2.018 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	2.166PheAla: 2.166 ± 0.019	0.798PheCys: 0.798 ± 0.012	2.072PheAsp: 2.072 ± 0.021	2.31PheGlu: 2.31 ± 0.021	1.566PhePhe: 1.566 ± 0.025	2.243PheGly: 2.243 ± 0.029	0.91PheHis: 0.91 ± 0.014	2.407PheIle: 2.407 ± 0.028	2.504PheLys: 2.504 ± 0.024	3.568PheLeu: 3.568 ± 0.038	0.944PheMet: 0.944 ± 0.013	2.263PheAsn: 2.263 ± 0.02	1.528PhePro: 1.528 ± 0.025	1.449PheGln: 1.449 ± 0.014	1.692PheArg: 1.692 ± 0.021	2.9PheSer: 2.9 ± 0.026	2.062PheThr: 2.062 ± 0.021	2.364PheVal: 2.364 ± 0.023	0.415PheTrp: 0.415 ± 0.01	1.449PheTyr: 1.449 ± 0.02	0.0PheXaa: 0.0 ± 0.0
Gly	3.068GlyAla: 3.068 ± 0.039	0.953GlyCys: 0.953 ± 0.019	2.582GlyAsp: 2.582 ± 0.031	2.676GlyGlu: 2.676 ± 0.027	2.162GlyPhe: 2.162 ± 0.026	4.99GlyGly: 4.99 ± 0.106	1.354GlyHis: 1.354 ± 0.019	2.891GlyIle: 2.891 ± 0.027	2.95GlyLys: 2.95 ± 0.026	4.17GlyLeu: 4.17 ± 0.037	1.077GlyMet: 1.077 ± 0.016	2.917GlyAsn: 2.917 ± 0.029	1.99GlyPro: 1.99 ± 0.035	1.865GlyGln: 1.865 ± 0.019	2.369GlyArg: 2.369 ± 0.029	4.538GlySer: 4.538 ± 0.048	2.701GlyThr: 2.701 ± 0.028	3.096GlyVal: 3.096 ± 0.03	0.519GlyTrp: 0.519 ± 0.012	1.826GlyTyr: 1.826 ± 0.029	0.0GlyXaa: 0.0 ± 0.0
His	1.248HisAla: 1.248 ± 0.018	0.531HisCys: 0.531 ± 0.012	1.131HisAsp: 1.131 ± 0.015	1.361HisGlu: 1.361 ± 0.016	1.037HisPhe: 1.037 ± 0.015	1.208HisGly: 1.208 ± 0.019	1.684HisHis: 1.684 ± 0.037	1.54HisIle: 1.54 ± 0.016	1.531HisLys: 1.531 ± 0.016	2.508HisLeu: 2.508 ± 0.023	0.675HisMet: 0.675 ± 0.012	1.63HisAsn: 1.63 ± 0.02	1.194HisPro: 1.194 ± 0.016	1.581HisGln: 1.581 ± 0.025	1.189HisArg: 1.189 ± 0.017	2.06HisSer: 2.06 ± 0.023	1.458HisThr: 1.458 ± 0.016	1.288HisVal: 1.288 ± 0.017	0.246HisTrp: 0.246 ± 0.006	0.94HisTyr: 0.94 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	3.423IleAla: 3.423 ± 0.028	1.399IleCys: 1.399 ± 0.022	3.121IleAsp: 3.121 ± 0.03	3.76IleGlu: 3.76 ± 0.034	2.513IlePhe: 2.513 ± 0.028	2.563IleGly: 2.563 ± 0.023	1.315IleHis: 1.315 ± 0.015	3.644IleIle: 3.644 ± 0.032	4.037IleLys: 4.037 ± 0.033	5.329IleLeu: 5.329 ± 0.048	1.367IleMet: 1.367 ± 0.017	3.589IleAsn: 3.589 ± 0.028	2.876IlePro: 2.876 ± 0.035	2.365IleGln: 2.365 ± 0.024	2.528IleArg: 2.528 ± 0.023	4.624IleSer: 4.624 ± 0.034	3.69IleThr: 3.69 ± 0.026	3.455IleVal: 3.455 ± 0.029	0.555IleTrp: 0.555 ± 0.01	1.994IleTyr: 1.994 ± 0.022	0.001IleXaa: 0.001 ± 0.0
Lys	3.527LysAla: 3.527 ± 0.032	1.39LysCys: 1.39 ± 0.027	3.414LysAsp: 3.414 ± 0.036	4.779LysGlu: 4.779 ± 0.058	2.433LysPhe: 2.433 ± 0.019	2.434LysGly: 2.434 ± 0.022	1.687LysHis: 1.687 ± 0.02	3.904LysIle: 3.904 ± 0.028	5.124LysLys: 5.124 ± 0.069	5.912LysLeu: 5.912 ± 0.04	1.592LysMet: 1.592 ± 0.018	3.692LysAsn: 3.692 ± 0.032	3.372LysPro: 3.372 ± 0.052	3.217LysGln: 3.217 ± 0.034	3.73LysArg: 3.73 ± 0.03	5.112LysSer: 5.112 ± 0.039	4.374LysThr: 4.374 ± 0.038	3.34LysVal: 3.34 ± 0.031	0.602LysTrp: 0.602 ± 0.01	2.308LysTyr: 2.308 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	5.554LeuAla: 5.554 ± 0.039	1.645LeuCys: 1.645 ± 0.022	4.443LeuAsp: 4.443 ± 0.036	5.929LeuGlu: 5.929 ± 0.046	3.129LeuPhe: 3.129 ± 0.033	4.005LeuGly: 4.005 ± 0.035	2.29LeuHis: 2.29 ± 0.023	4.807LeuIle: 4.807 ± 0.041	6.724LeuLys: 6.724 ± 0.045	8.37LeuLeu: 8.37 ± 0.067	2.143LeuMet: 2.143 ± 0.022	5.513LeuAsn: 5.513 ± 0.035	4.564LeuPro: 4.564 ± 0.031	4.992LeuGln: 4.992 ± 0.04	4.515LeuArg: 4.515 ± 0.033	6.714LeuSer: 6.714 ± 0.045	5.276LeuThr: 5.276 ± 0.029	4.553LeuVal: 4.553 ± 0.033	0.823LeuTrp: 0.823 ± 0.012	2.682LeuTyr: 2.682 ± 0.025	0.0LeuXaa: 0.0 ± 0.0
Met	1.649MetAla: 1.649 ± 0.016	0.432MetCys: 0.432 ± 0.009	1.372MetAsp: 1.372 ± 0.016	1.676MetGlu: 1.676 ± 0.017	0.885MetPhe: 0.885 ± 0.014	1.287MetGly: 1.287 ± 0.018	0.579MetHis: 0.579 ± 0.009	1.1MetIle: 1.1 ± 0.014	1.484MetLys: 1.484 ± 0.017	2.005MetLeu: 2.005 ± 0.02	0.588MetMet: 0.588 ± 0.011	1.177MetAsn: 1.177 ± 0.015	1.239MetPro: 1.239 ± 0.016	1.174MetGln: 1.174 ± 0.015	1.14MetArg: 1.14 ± 0.015	1.868MetSer: 1.868 ± 0.016	1.235MetThr: 1.235 ± 0.016	1.2MetVal: 1.2 ± 0.014	0.216MetTrp: 0.216 ± 0.006	0.691MetTyr: 0.691 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	3.506AsnAla: 3.506 ± 0.03	1.193AsnCys: 1.193 ± 0.019	3.297AsnAsp: 3.297 ± 0.027	3.858AsnGlu: 3.858 ± 0.032	2.292AsnPhe: 2.292 ± 0.025	3.283AsnGly: 3.283 ± 0.035	1.403AsnHis: 1.403 ± 0.02	4.013AsnIle: 4.013 ± 0.031	3.88AsnLys: 3.88 ± 0.033	5.178AsnLeu: 5.178 ± 0.035	1.47AsnMet: 1.47 ± 0.014	5.95AsnAsn: 5.95 ± 0.075	2.475AsnPro: 2.475 ± 0.027	2.33AsnGln: 2.33 ± 0.024	2.362AsnArg: 2.362 ± 0.021	5.611AsnSer: 5.611 ± 0.055	4.046AsnThr: 4.046 ± 0.035	3.386AsnVal: 3.386 ± 0.028	0.528AsnTrp: 0.528 ± 0.009	2.033AsnTyr: 2.033 ± 0.02	0.0AsnXaa: 0.0 ± 0.0
Pro	3.262ProAla: 3.262 ± 0.04	0.73ProCys: 0.73 ± 0.045	2.107ProAsp: 2.107 ± 0.022	3.006ProGlu: 3.006 ± 0.042	1.622ProPhe: 1.622 ± 0.022	2.203ProGly: 2.203 ± 0.035	1.229ProHis: 1.229 ± 0.017	2.65ProIle: 2.65 ± 0.034	3.049ProLys: 3.049 ± 0.039	4.203ProLeu: 4.203 ± 0.029	0.989ProMet: 0.989 ± 0.013	2.661ProAsn: 2.661 ± 0.033	4.09ProPro: 4.09 ± 0.069	2.58ProGln: 2.58 ± 0.031	1.901ProArg: 1.901 ± 0.023	4.133ProSer: 4.133 ± 0.052	3.553ProThr: 3.553 ± 0.043	2.852ProVal: 2.852 ± 0.037	0.4ProTrp: 0.4 ± 0.01	1.48ProTyr: 1.48 ± 0.02	0.0ProXaa: 0.0 ± 0.0
Gln	2.466GlnAla: 2.466 ± 0.025	0.798GlnCys: 0.798 ± 0.02	1.787GlnAsp: 1.787 ± 0.02	2.887GlnGlu: 2.887 ± 0.035	1.535GlnPhe: 1.535 ± 0.016	1.62GlnGly: 1.62 ± 0.021	1.974GlnHis: 1.974 ± 0.03	2.525GlnIle: 2.525 ± 0.024	2.99GlnLys: 2.99 ± 0.032	4.377GlnLeu: 4.377 ± 0.033	1.229GlnMet: 1.229 ± 0.016	2.805GlnAsn: 2.805 ± 0.026	2.38GlnPro: 2.38 ± 0.033	7.557GlnGln: 7.557 ± 0.166	2.557GlnArg: 2.557 ± 0.024	3.06GlnSer: 3.06 ± 0.031	2.958GlnThr: 2.958 ± 0.028	2.063GlnVal: 2.063 ± 0.022	0.395GlnTrp: 0.395 ± 0.008	1.45GlnTyr: 1.45 ± 0.017	0.0GlnXaa: 0.0 ± 0.0
Arg	2.344ArgAla: 2.344 ± 0.024	0.882ArgCys: 0.882 ± 0.017	2.356ArgAsp: 2.356 ± 0.023	2.937ArgGlu: 2.937 ± 0.035	1.806ArgPhe: 1.806 ± 0.019	2.144ArgGly: 2.144 ± 0.027	1.348ArgHis: 1.348 ± 0.016	2.618ArgIle: 2.618 ± 0.023	3.578ArgLys: 3.578 ± 0.027	4.202ArgLeu: 4.202 ± 0.03	0.988ArgMet: 0.988 ± 0.013	2.776ArgAsn: 2.776 ± 0.023	2.152ArgPro: 2.152 ± 0.03	2.221ArgGln: 2.221 ± 0.022	3.291ArgArg: 3.291 ± 0.041	3.727ArgSer: 3.727 ± 0.041	2.431ArgThr: 2.431 ± 0.022	2.28ArgVal: 2.28 ± 0.021	0.42ArgTrp: 0.42 ± 0.008	1.563ArgTyr: 1.563 ± 0.018	0.0ArgXaa: 0.0 ± 0.0
Ser	5.12SerAla: 5.12 ± 0.046	1.44SerCys: 1.44 ± 0.032	3.854SerAsp: 3.854 ± 0.036	4.377SerGlu: 4.377 ± 0.044	2.875SerPhe: 2.875 ± 0.027	4.524SerGly: 4.524 ± 0.043	1.893SerHis: 1.893 ± 0.025	4.399SerIle: 4.399 ± 0.028	4.817SerLys: 4.817 ± 0.034	7.265SerLeu: 7.265 ± 0.041	1.73SerMet: 1.73 ± 0.019	5.453SerAsn: 5.453 ± 0.046	4.334SerPro: 4.334 ± 0.061	3.318SerGln: 3.318 ± 0.029	3.33SerArg: 3.33 ± 0.045	11.118SerSer: 11.118 ± 0.133	6.262SerThr: 6.262 ± 0.063	4.253SerVal: 4.253 ± 0.033	0.679SerTrp: 0.679 ± 0.012	2.402SerTyr: 2.402 ± 0.029	0.0SerXaa: 0.0 ± 0.0
Thr	4.662ThrAla: 4.662 ± 0.036	1.079ThrCys: 1.079 ± 0.022	2.842ThrAsp: 2.842 ± 0.034	3.662ThrGlu: 3.662 ± 0.045	2.183ThrPhe: 2.183 ± 0.023	3.049ThrGly: 3.049 ± 0.031	1.443ThrHis: 1.443 ± 0.017	3.642ThrIle: 3.642 ± 0.024	3.904ThrLys: 3.904 ± 0.044	5.266ThrLeu: 5.266 ± 0.03	1.297ThrMet: 1.297 ± 0.016	4.119ThrAsn: 4.119 ± 0.036	3.872ThrPro: 3.872 ± 0.046	2.684ThrGln: 2.684 ± 0.026	2.258ThrArg: 2.258 ± 0.028	6.135ThrSer: 6.135 ± 0.052	7.353ThrThr: 7.353 ± 0.154	3.464ThrVal: 3.464 ± 0.033	0.498ThrTrp: 0.498 ± 0.013	1.69ThrTyr: 1.69 ± 0.018	0.0ThrXaa: 0.0 ± 0.0
Val	4.007ValAla: 4.007 ± 0.035	1.177ValCys: 1.177 ± 0.024	2.981ValAsp: 2.981 ± 0.027	3.712ValGlu: 3.712 ± 0.041	2.148ValPhe: 2.148 ± 0.024	2.976ValGly: 2.976 ± 0.026	1.29ValHis: 1.29 ± 0.017	3.237ValIle: 3.237 ± 0.027	3.617ValLys: 3.617 ± 0.036	4.946ValLeu: 4.946 ± 0.035	1.246ValMet: 1.246 ± 0.016	3.048ValAsn: 3.048 ± 0.024	2.645ValPro: 2.645 ± 0.022	2.313ValGln: 2.313 ± 0.025	2.405ValArg: 2.405 ± 0.024	3.992ValSer: 3.992 ± 0.034	3.404ValThr: 3.404 ± 0.029	4.009ValVal: 4.009 ± 0.049	0.544ValTrp: 0.544 ± 0.011	1.753ValTyr: 1.753 ± 0.022	0.0ValXaa: 0.0 ± 0.0
Trp	0.441TrpAla: 0.441 ± 0.009	0.201TrpCys: 0.201 ± 0.006	0.446TrpAsp: 0.446 ± 0.009	0.496TrpGlu: 0.496 ± 0.009	0.404TrpPhe: 0.404 ± 0.009	0.482TrpGly: 0.482 ± 0.011	0.262TrpHis: 0.262 ± 0.007	0.562TrpIle: 0.562 ± 0.011	0.596TrpLys: 0.596 ± 0.012	0.973TrpLeu: 0.973 ± 0.015	0.227TrpMet: 0.227 ± 0.006	0.516TrpAsn: 0.516 ± 0.01	0.373TrpPro: 0.373 ± 0.009	0.449TrpGln: 0.449 ± 0.009	0.531TrpArg: 0.531 ± 0.009	0.753TrpSer: 0.753 ± 0.013	0.467TrpThr: 0.467 ± 0.009	0.435TrpVal: 0.435 ± 0.008	0.148TrpTrp: 0.148 ± 0.006	0.323TrpTyr: 0.323 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.86TyrAla: 1.86 ± 0.024	0.731TyrCys: 0.731 ± 0.012	1.846TyrAsp: 1.846 ± 0.019	2.024TyrGlu: 2.024 ± 0.019	1.443TyrPhe: 1.443 ± 0.02	1.833TyrGly: 1.833 ± 0.028	0.885TyrHis: 0.885 ± 0.013	1.847TyrIle: 1.847 ± 0.022	1.989TyrLys: 1.989 ± 0.022	2.865TyrLeu: 2.865 ± 0.027	0.792TyrMet: 0.792 ± 0.011	2.005TyrAsn: 2.005 ± 0.021	1.36TyrPro: 1.36 ± 0.019	1.403TyrGln: 1.403 ± 0.016	1.612TyrArg: 1.612 ± 0.018	2.331TyrSer: 2.331 ± 0.025	1.82TyrThr: 1.82 ± 0.022	1.779TyrVal: 1.779 ± 0.021	0.358TyrTrp: 0.358 ± 0.008	1.331TyrTyr: 1.331 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.075XaaXaa: 0.075 ± 0.017

Statistics based on 14353 proteins (7420241 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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