Paraphaeosphaeria sporulosa

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Pleosporomycetidae; Pleosporales; Massarineae; Didymosphaeriaceae; Paraphaeosphaeria

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14732 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A177CH65|A0A177CH65_9PLEO Alkaline phosphatase OS=Paraphaeosphaeria sporulosa OX=1460663 GN=CC84DRAFT_1090178 PE=3 SV=1
MM1 pKa = 7.79AYY3 pKa = 7.9ITLFSSLLPATLFFSPAQAADD24 pKa = 3.75CNLAQSRR31 pKa = 11.84RR32 pKa = 11.84SGDD35 pKa = 3.32PSGAAIASDD44 pKa = 5.36LRR46 pKa = 11.84GQDD49 pKa = 3.15NALLQSVCNGGFPPGSDD66 pKa = 5.17IISTWNTGSQIYY78 pKa = 10.4NVTRR82 pKa = 11.84FDD84 pKa = 3.55SSQPLQYY91 pKa = 10.96CFDD94 pKa = 4.04AFDD97 pKa = 5.11NIITQCIEE105 pKa = 3.54NDD107 pKa = 3.86NYY109 pKa = 10.78WGGDD113 pKa = 2.92WKK115 pKa = 10.99LGNEE119 pKa = 4.14IYY121 pKa = 10.5AIYY124 pKa = 10.52DD125 pKa = 3.75STWPEE130 pKa = 3.73HH131 pKa = 5.45VLEE134 pKa = 5.52AEE136 pKa = 4.42LEE138 pKa = 4.41SPPSSSAQDD147 pKa = 3.37VSPSSTPDD155 pKa = 3.07PPARR159 pKa = 11.84ATVVTTTIDD168 pKa = 3.5GFPITQTFVPTTIADD183 pKa = 4.1ASPTTSSTTVDD194 pKa = 3.54GVVVPLIIGAGGVAWIPFVPVGGAPPAVITPPADD228 pKa = 3.71LPDD231 pKa = 4.29GGNGDD236 pKa = 4.23GDD238 pKa = 4.85DD239 pKa = 4.43NSDD242 pKa = 3.84SQSSAPEE249 pKa = 4.16STSSSSSSSSSRR261 pKa = 11.84ALRR264 pKa = 11.84APDD267 pKa = 3.9DD268 pKa = 4.13VFPADD273 pKa = 3.88AGTATFDD280 pKa = 3.45SGAAAQIVFFGAAAPTPTPSSSSEE304 pKa = 3.59ASAEE308 pKa = 3.92PTVDD312 pKa = 3.52SNAEE316 pKa = 3.96AKK318 pKa = 10.07KK319 pKa = 10.44LCHH322 pKa = 5.98EE323 pKa = 4.52RR324 pKa = 11.84CGSKK328 pKa = 10.38PGLTTSDD335 pKa = 4.01GQWCLANCGCFLGAAGCC352 pKa = 4.03

Molecular weight: 36.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A177C192|A0A177C192_9PLEO Uncharacterized protein OS=Paraphaeosphaeria sporulosa OX=1460663 GN=CC84DRAFT_1263047 PE=4 SV=1
MM1 pKa = 7.74PPMPKK6 pKa = 9.43RR7 pKa = 11.84RR8 pKa = 11.84GHH10 pKa = 5.51SFPRR14 pKa = 11.84LKK16 pKa = 10.56SKK18 pKa = 10.09PRR20 pKa = 11.84RR21 pKa = 11.84PSLPPSPPRR30 pKa = 11.84RR31 pKa = 11.84TKK33 pKa = 10.9LSTPSIRR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84SSWTASWLRR51 pKa = 11.84SSKK54 pKa = 9.64SWMPRR59 pKa = 11.84RR60 pKa = 11.84PATAKK65 pKa = 10.38LSKK68 pKa = 10.16SYY70 pKa = 9.22QARR73 pKa = 11.84TRR75 pKa = 11.84STPSCRR81 pKa = 11.84KK82 pKa = 7.66ITLLVVSKK90 pKa = 11.38AMVTRR95 pKa = 11.84PAFRR99 pKa = 11.84SWKK102 pKa = 9.35PQLQALNRR110 pKa = 11.84PRR112 pKa = 11.84VMFPAKK118 pKa = 10.02SQHH121 pKa = 6.13CKK123 pKa = 10.3SLSLRR128 pKa = 11.84RR129 pKa = 11.84RR130 pKa = 11.84RR131 pKa = 11.84LL132 pKa = 3.15

Molecular weight: 15.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14732

0

14732

6378640

49

7206

433.0

47.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.051 ± 0.016

1.316 ± 0.009

5.531 ± 0.015

5.984 ± 0.019

3.746 ± 0.013

6.976 ± 0.021

2.473 ± 0.009

4.775 ± 0.013

4.883 ± 0.018

8.747 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.166 ± 0.008

3.623 ± 0.011

6.108 ± 0.024

3.93 ± 0.015

6.11 ± 0.019

7.948 ± 0.022

6.054 ± 0.013

6.181 ± 0.016

1.567 ± 0.008

2.831 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski