Saimiriine herpesvirus 1 (strain MV-5-4-PSL) (SaHV-1) (Marmoset herpesvirus)
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E2IUG7|E2IUG7_SHV1 Nuclear protein UL3 OS=Saimiriine herpesvirus 1 (strain MV-5-4-PSL) OX=10353 GN=UL03 PE=3 SV=1
MM1 pKa = 7.98 DD2 pKa = 5.85 PSSQLSLVAYY12 pKa = 6.88 TLRR15 pKa = 11.84 NARR18 pKa = 11.84 GPTTWKK24 pKa = 10.71 LYY26 pKa = 8.71 DD27 pKa = 3.53 TEE29 pKa = 5.8 HH30 pKa = 6.53 IVSTFDD36 pKa = 2.99 SGVRR40 pKa = 11.84 YY41 pKa = 9.52 VATAGRR47 pKa = 11.84 LRR49 pKa = 11.84 QDD51 pKa = 3.64 PLDD54 pKa = 4.19 GGSVVLQDD62 pKa = 3.5 TPGGVVVVVNCPADD76 pKa = 3.81 FCAYY80 pKa = 9.83 RR81 pKa = 11.84 FVGRR85 pKa = 11.84 TQGHH89 pKa = 5.97 VLRR92 pKa = 11.84 QWEE95 pKa = 4.23 DD96 pKa = 2.58 TGMCVYY102 pKa = 10.5 PFDD105 pKa = 3.55 AWTGSTHH112 pKa = 5.26 TEE114 pKa = 4.12 SVRR117 pKa = 11.84 SASAGTAIVLWCDD130 pKa = 3.04 DD131 pKa = 3.75 SLYY134 pKa = 9.51 ITATVCDD141 pKa = 4.67 ADD143 pKa = 4.51 PPADD147 pKa = 3.75 SPTARR152 pKa = 11.84 VDD154 pKa = 3.5 EE155 pKa = 4.72 TPGSLLEE162 pKa = 4.64 DD163 pKa = 3.16 AHH165 pKa = 6.88 EE166 pKa = 4.33 EE167 pKa = 3.96 LLAGDD172 pKa = 3.75 SEE174 pKa = 4.6 ARR176 pKa = 11.84 VAADD180 pKa = 4.38 LLTEE184 pKa = 4.22 VLDD187 pKa = 4.06 QVQLGPASSFEE198 pKa = 3.97 GCARR202 pKa = 11.84 GAQQ205 pKa = 3.4
Molecular weight: 21.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.141
IPC2_protein 4.24
IPC_protein 4.215
Toseland 4.012
ProMoST 4.368
Dawson 4.202
Bjellqvist 4.342
Wikipedia 4.126
Rodwell 4.037
Grimsley 3.923
Solomon 4.19
Lehninger 4.151
Nozaki 4.304
DTASelect 4.546
Thurlkill 4.05
EMBOSS 4.139
Sillero 4.329
Patrickios 3.058
IPC_peptide 4.19
IPC2_peptide 4.317
IPC2.peptide.svr19 4.235
Protein with the highest isoelectric point:
>tr|E2IUG9|E2IUG9_SHV1 Envelope glycoprotein L OS=Saimiriine herpesvirus 1 (strain MV-5-4-PSL) OX=10353 GN=UL01 PE=3 SV=1
MM1 pKa = 7.39 KK2 pKa = 10.32 RR3 pKa = 11.84 QATPDD8 pKa = 3.39 SRR10 pKa = 11.84 QLTLLAPQLPSPSNHH25 pKa = 6.63 PPPLPEE31 pKa = 3.82 SRR33 pKa = 11.84 RR34 pKa = 11.84 GAEE37 pKa = 3.98 DD38 pKa = 3.58 PKK40 pKa = 10.85 TEE42 pKa = 4.21 GSSAPDD48 pKa = 3.49 VIEE51 pKa = 4.58 PPLAAVAKK59 pKa = 9.86 RR60 pKa = 11.84 PRR62 pKa = 11.84 RR63 pKa = 11.84 PRR65 pKa = 11.84 GCPLGVQFGGSPSAADD81 pKa = 3.1 AVEE84 pKa = 4.65 RR85 pKa = 11.84 EE86 pKa = 4.35 TASLFSPSSGVSDD99 pKa = 3.64 RR100 pKa = 11.84 TLDD103 pKa = 3.08 WRR105 pKa = 11.84 RR106 pKa = 11.84 ARR108 pKa = 11.84 DD109 pKa = 3.35 VFGIGEE115 pKa = 4.17 PWRR118 pKa = 11.84 DD119 pKa = 3.53 VIEE122 pKa = 4.76 PEE124 pKa = 3.88 LAAAATSGLLSEE136 pKa = 4.38 YY137 pKa = 10.13 FRR139 pKa = 11.84 RR140 pKa = 11.84 CRR142 pKa = 11.84 TEE144 pKa = 3.88 EE145 pKa = 3.85 VLPPRR150 pKa = 11.84 GDD152 pKa = 3.34 VFSWTRR158 pKa = 11.84 SCAPDD163 pKa = 3.55 DD164 pKa = 3.67 VRR166 pKa = 11.84 VVIIGQDD173 pKa = 3.65 PYY175 pKa = 10.43 HH176 pKa = 6.66 QPGQAHH182 pKa = 6.65 GLAFSVRR189 pKa = 11.84 PGVPVPPSLRR199 pKa = 11.84 NIFTAVRR206 pKa = 11.84 SCYY209 pKa = 10.14 PEE211 pKa = 3.88 ATPAAGHH218 pKa = 6.2 GCLEE222 pKa = 3.62 KK223 pKa = 10.01 WARR226 pKa = 11.84 QGVLLLNTTLTVRR239 pKa = 11.84 RR240 pKa = 11.84 GAAGSHH246 pKa = 6.36 SGLGWGRR253 pKa = 11.84 FVAGVLRR260 pKa = 11.84 RR261 pKa = 11.84 LAQRR265 pKa = 11.84 RR266 pKa = 11.84 DD267 pKa = 3.28 GLVFMLWGQHH277 pKa = 4.63 AQGAIKK283 pKa = 10.28 PDD285 pKa = 3.77 TQRR288 pKa = 11.84 HH289 pKa = 4.23 LVLRR293 pKa = 11.84 FAHH296 pKa = 6.86 PSPLSQTPFGTCRR309 pKa = 11.84 HH310 pKa = 5.8 FLLANQYY317 pKa = 10.71 LEE319 pKa = 4.95 ARR321 pKa = 11.84 GLPPIDD327 pKa = 3.68 WSLGPAPSLARR338 pKa = 11.84 AKK340 pKa = 10.59
Molecular weight: 36.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.316
IPC2_protein 9.136
IPC_protein 9.648
Toseland 10.058
ProMoST 9.926
Dawson 10.248
Bjellqvist 10.028
Wikipedia 10.452
Rodwell 10.306
Grimsley 10.306
Solomon 10.335
Lehninger 10.292
Nozaki 10.189
DTASelect 9.984
Thurlkill 10.116
EMBOSS 10.482
Sillero 10.204
Patrickios 9.882
IPC_peptide 10.335
IPC2_peptide 9.355
IPC2.peptide.svr19 8.162
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
68
1
69
38775
76
3390
562.0
60.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.025 ± 0.557
1.782 ± 0.115
5.638 ± 0.175
5.594 ± 0.155
3.317 ± 0.187
7.319 ± 0.283
2.218 ± 0.107
2.507 ± 0.173
1.666 ± 0.118
9.439 ± 0.254
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.586 ± 0.097
1.95 ± 0.163
7.892 ± 0.445
2.545 ± 0.143
9.32 ± 0.28
7.51 ± 0.227
5.207 ± 0.196
6.894 ± 0.241
1.13 ± 0.065
2.463 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here