Rhodosporidium toruloides (Yeast) (Rhodotorula gracilis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota;

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7496 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K3C6Z5|A0A0K3C6Z5_RHOTO FGENESH: predicted gene_1.91 protein (Fragment) OS=Rhodosporidium toruloides OX=5286 GN=FGENESH: predicted gene_1.91 PE=4 SV=1
MM1 pKa = 7.62GLLDD5 pKa = 3.84KK6 pKa = 11.3AKK8 pKa = 10.59DD9 pKa = 3.99AISHH13 pKa = 6.64HH14 pKa = 5.59GQNKK18 pKa = 9.8NSGSDD23 pKa = 3.27NYY25 pKa = 11.29NDD27 pKa = 3.38NTTGDD32 pKa = 4.1SYY34 pKa = 12.09NSGNQSYY41 pKa = 8.93GQQDD45 pKa = 3.47SSFGGASSGFDD56 pKa = 3.33STSDD60 pKa = 3.25RR61 pKa = 11.84YY62 pKa = 10.82GSSDD66 pKa = 3.38NQGSGGFTSGDD77 pKa = 3.42NNNNQDD83 pKa = 3.78DD84 pKa = 4.25SFSNSRR90 pKa = 11.84RR91 pKa = 11.84AGAAEE96 pKa = 3.9GDD98 pKa = 4.23SYY100 pKa = 11.98GGASGGFDD108 pKa = 3.33STSDD112 pKa = 3.4RR113 pKa = 11.84YY114 pKa = 11.02GSGGNDD120 pKa = 3.36DD121 pKa = 4.72SYY123 pKa = 12.08GSGGAGRR130 pKa = 11.84SSGLGANDD138 pKa = 3.44MTSGRR143 pKa = 11.84QSDD146 pKa = 4.08FDD148 pKa = 4.44SGSQSYY154 pKa = 10.94GDD156 pKa = 3.56NSYY159 pKa = 11.36GSNRR163 pKa = 11.84ASGFDD168 pKa = 3.57DD169 pKa = 4.4ASNISDD175 pKa = 3.28TGNRR179 pKa = 11.84LDD181 pKa = 3.38SSYY184 pKa = 11.58GGGSGRR190 pKa = 11.84GTDD193 pKa = 4.27FQASGGGSAYY203 pKa = 10.51GDD205 pKa = 3.64GQTDD209 pKa = 3.82FGGAGRR215 pKa = 11.84NNDD218 pKa = 3.2GQYY221 pKa = 11.09GDD223 pKa = 4.04SSFGGSSGNQGYY235 pKa = 8.21GQDD238 pKa = 3.32QTQYY242 pKa = 11.18SGGGNQYY249 pKa = 10.63GGEE252 pKa = 3.97RR253 pKa = 11.84DD254 pKa = 3.52NYY256 pKa = 9.33PP257 pKa = 3.18

Molecular weight: 25.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K3C6B7|A0A0K3C6B7_RHOTO BY PROTMAP: gi|342319937|gb|EGU11882.1| Proteophosphoglycan 5 [Rhodotorula glutinis ATCC 204091] OS=Rhodosporidium toruloides OX=5286 GN=FGENESH: predicted gene_2.254 PE=4 SV=1
MM1 pKa = 7.36MLNALRR7 pKa = 11.84SASRR11 pKa = 11.84TLSRR15 pKa = 11.84TVQIAGARR23 pKa = 11.84SPLAAPSHH31 pKa = 6.23SRR33 pKa = 11.84LISTSSSALSPILSTAASRR52 pKa = 11.84LTSAAARR59 pKa = 11.84PQTSLLQRR67 pKa = 11.84EE68 pKa = 4.43LAQAGTRR75 pKa = 11.84PGFWHH80 pKa = 6.96TSPSPLSSAVQQQVRR95 pKa = 11.84GYY97 pKa = 11.12KK98 pKa = 8.93MPKK101 pKa = 8.81CMRR104 pKa = 11.84RR105 pKa = 11.84KK106 pKa = 9.95ASPLARR112 pKa = 11.84NGGKK116 pKa = 10.51GKK118 pKa = 6.7TARR121 pKa = 11.84RR122 pKa = 11.84KK123 pKa = 10.55GMLKK127 pKa = 10.08AKK129 pKa = 9.45RR130 pKa = 11.84RR131 pKa = 11.84RR132 pKa = 11.84MRR134 pKa = 11.84IKK136 pKa = 10.51KK137 pKa = 10.05INN139 pKa = 3.2

Molecular weight: 15.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7496

0

7496

4252312

50

5355

567.3

61.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.691 ± 0.031

0.998 ± 0.012

5.499 ± 0.019

6.381 ± 0.027

3.41 ± 0.016

7.116 ± 0.027

2.207 ± 0.01

3.596 ± 0.02

4.417 ± 0.023

9.372 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.668 ± 0.008

2.559 ± 0.015

7.158 ± 0.038

3.63 ± 0.02

6.738 ± 0.025

9.071 ± 0.039

5.735 ± 0.019

6.19 ± 0.019

1.287 ± 0.009

2.276 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski