Beihai picorna-like virus 113
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KN85|A0A1L3KN85_9VIRU Uncharacterized protein OS=Beihai picorna-like virus 113 OX=1922542 PE=4 SV=1
MM1 pKa = 7.68 SSTKK5 pKa = 9.83 TNPPTGVIDD14 pKa = 5.32 DD15 pKa = 4.13 NATLEE20 pKa = 4.24 NFSKK24 pKa = 10.73 EE25 pKa = 4.02 VVGEE29 pKa = 3.91 KK30 pKa = 10.32 RR31 pKa = 11.84 LAAKK35 pKa = 8.85 PASTIPAVRR44 pKa = 11.84 GPYY47 pKa = 9.79 SSSKK51 pKa = 10.35 MMTPDD56 pKa = 3.28 GLVDD60 pKa = 3.84 VYY62 pKa = 11.07 SFSAIPSKK70 pKa = 10.71 PLIDD74 pKa = 3.86 PSKK77 pKa = 11.32 VNTATNPLYY86 pKa = 10.56 HH87 pKa = 6.51 HH88 pKa = 7.0 WEE90 pKa = 4.07 HH91 pKa = 6.65 YY92 pKa = 7.39 RR93 pKa = 11.84 TQSITATYY101 pKa = 9.01 LQRR104 pKa = 11.84 FEE106 pKa = 6.05 FSQQDD111 pKa = 3.23 HH112 pKa = 5.68 STLNKK117 pKa = 8.28 ICSFYY122 pKa = 10.63 RR123 pKa = 11.84 YY124 pKa = 7.8 NTGGMIVKK132 pKa = 10.38 LKK134 pKa = 10.77 LQTTEE139 pKa = 4.06 LEE141 pKa = 4.45 GVEE144 pKa = 4.19 LAALSLSKK152 pKa = 10.69 HH153 pKa = 5.53 LAHH156 pKa = 7.26 KK157 pKa = 10.84 GEE159 pKa = 4.48 DD160 pKa = 3.26 VGGIPTLKK168 pKa = 9.45 WVPSEE173 pKa = 3.82 QPVIYY178 pKa = 8.94 FHH180 pKa = 7.07 IPQDD184 pKa = 3.2 AEE186 pKa = 4.02 YY187 pKa = 10.18 NYY189 pKa = 10.74 NPEE192 pKa = 4.12 LQLPSLILACVRR204 pKa = 11.84 FAQVKK209 pKa = 10.21 GMLDD213 pKa = 3.47 PQLQMWISPAPEE225 pKa = 3.79 VRR227 pKa = 11.84 HH228 pKa = 5.8 HH229 pKa = 6.41 FRR231 pKa = 11.84 VDD233 pKa = 3.15 VPKK236 pKa = 10.74 EE237 pKa = 3.65 VDD239 pKa = 3.71 FDD241 pKa = 4.05 YY242 pKa = 11.13 LKK244 pKa = 10.35 TIIEE248 pKa = 4.46 PEE250 pKa = 3.94 WFTEE254 pKa = 4.4 DD255 pKa = 3.32 NKK257 pKa = 10.36 CTLTYY262 pKa = 10.1 HH263 pKa = 6.92 PSSGATYY270 pKa = 8.98 SQPFFSLVAADD281 pKa = 3.94 SGATIKK287 pKa = 10.9 DD288 pKa = 3.44 SLEE291 pKa = 3.91 QSVCEE296 pKa = 3.95 MLTDD300 pKa = 3.57 YY301 pKa = 10.86 SYY303 pKa = 11.71 EE304 pKa = 4.17 GDD306 pKa = 3.34 TTLTNEE312 pKa = 4.13 QKK314 pKa = 9.78 FTIHH318 pKa = 7.28 CITKK322 pKa = 9.57 PMTPIHH328 pKa = 5.27 VHH330 pKa = 5.45 VIKK333 pKa = 10.24 IGHH336 pKa = 5.38 VAAEE340 pKa = 4.13 LGYY343 pKa = 9.89 PSFRR347 pKa = 11.84 RR348 pKa = 11.84 TKK350 pKa = 8.06 MQVGEE355 pKa = 4.23 FQTPSDD361 pKa = 3.66 QVGPAFTGLNSWVALTKK378 pKa = 10.33 FALNSSVDD386 pKa = 3.33 VKK388 pKa = 11.39 YY389 pKa = 10.89 IDD391 pKa = 3.74 ITPKK395 pKa = 9.95 MLSQLLQGRR404 pKa = 11.84 LLSSRR409 pKa = 11.84 LFFNGTLHH417 pKa = 6.5 CRR419 pKa = 11.84 VSTKK423 pKa = 10.39 VVLTNYY429 pKa = 9.33 QAYY432 pKa = 10.03 LVILPPGARR441 pKa = 11.84 LSTDD445 pKa = 2.83 NDD447 pKa = 3.75 TAASQALHH455 pKa = 6.38 LRR457 pKa = 11.84 PIDD460 pKa = 3.54 MDD462 pKa = 3.81 SSHH465 pKa = 6.24 QSKK468 pKa = 8.09 TFPIMNMTPKK478 pKa = 10.64 NFVTYY483 pKa = 9.72 EE484 pKa = 3.79 EE485 pKa = 4.64 SFATLAIVFDD495 pKa = 4.07 NKK497 pKa = 10.63 AFRR500 pKa = 11.84 LNMTSTNPEE509 pKa = 3.67 MLIEE513 pKa = 4.67 LNLSSFRR520 pKa = 11.84 GHH522 pKa = 4.68 VQGAKK527 pKa = 10.19 SSDD530 pKa = 2.83 WGKK533 pKa = 10.71 AFQPARR539 pKa = 11.84 KK540 pKa = 8.06 QAAVAAEE547 pKa = 4.12 AGEE550 pKa = 4.19 LALGEE555 pKa = 4.14 IVEE558 pKa = 4.44 EE559 pKa = 4.27 VGLDD563 pKa = 3.35 EE564 pKa = 5.5 GLGSLTGSTSTLVGEE579 pKa = 4.41 EE580 pKa = 4.23 VSPKK584 pKa = 10.34 GAGLGDD590 pKa = 4.69 HH591 pKa = 7.02 ILGNLTDD598 pKa = 4.74 DD599 pKa = 3.78 VMSGVDD605 pKa = 3.48 SKK607 pKa = 11.63 VSSVFNPGYY616 pKa = 10.39 HH617 pKa = 6.41 KK618 pKa = 10.58 PNEE621 pKa = 4.0 GAIDD625 pKa = 3.7 KK626 pKa = 10.54 DD627 pKa = 4.1 FSGLEE632 pKa = 3.81 QRR634 pKa = 11.84 LDD636 pKa = 3.34 TDD638 pKa = 5.2 LEE640 pKa = 4.24 MLDD643 pKa = 4.94 SSIQIQGQKK652 pKa = 7.17 TLKK655 pKa = 10.75 ALGAIDD661 pKa = 3.6 KK662 pKa = 9.35 TLAAGFGQQASVGGSVICEE681 pKa = 3.88 DD682 pKa = 4.53 DD683 pKa = 3.21 TWHH686 pKa = 6.03 MLEE689 pKa = 4.33 VATISKK695 pKa = 11.0 GEE697 pKa = 4.13 TKK699 pKa = 10.58 LVDD702 pKa = 4.53 LSPFAAEE709 pKa = 4.96 DD710 pKa = 3.53 SLAPMAGYY718 pKa = 9.08 MNHH721 pKa = 6.55 HH722 pKa = 6.52 ATWFSGVPGVGIKK735 pKa = 9.83 ILAAAGLATAEE746 pKa = 4.39 VFTTPLTNDD755 pKa = 3.38 ALIKK759 pKa = 11.04 SNAKK763 pKa = 10.0 YY764 pKa = 9.67 YY765 pKa = 10.99 AKK767 pKa = 9.77 TYY769 pKa = 10.96 VSGQSMQKK777 pKa = 9.78 IPTNWFLPQQRR788 pKa = 11.84 ADD790 pKa = 3.26 ATAVRR795 pKa = 11.84 DD796 pKa = 3.53 SGRR799 pKa = 11.84 VVISAPGGDD808 pKa = 3.35 ITVLIYY814 pKa = 11.03 KK815 pKa = 9.67 NFSQKK820 pKa = 10.33 YY821 pKa = 7.77 VLASGVRR828 pKa = 11.84 SEE830 pKa = 4.58 FEE832 pKa = 3.85 KK833 pKa = 11.17
Molecular weight: 91.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.527
IPC2_protein 5.448
IPC_protein 5.486
Toseland 5.855
ProMoST 5.792
Dawson 5.69
Bjellqvist 5.715
Wikipedia 5.664
Rodwell 5.652
Grimsley 6.02
Solomon 5.69
Lehninger 5.664
Nozaki 5.919
DTASelect 6.109
Thurlkill 6.071
EMBOSS 6.033
Sillero 6.02
Patrickios 4.075
IPC_peptide 5.703
IPC2_peptide 6.02
IPC2.peptide.svr19 5.973
Protein with the highest isoelectric point:
>tr|A0A1L3KN85|A0A1L3KN85_9VIRU Uncharacterized protein OS=Beihai picorna-like virus 113 OX=1922542 PE=4 SV=1
MM1 pKa = 7.42 LVEE4 pKa = 4.51 DD5 pKa = 4.88 LNVFVQGTLPHH16 pKa = 6.8 DD17 pKa = 4.1 SYY19 pKa = 12.23 YY20 pKa = 11.33 KK21 pKa = 9.74 LTPPLPIFFLFLISFFLFLAEE42 pKa = 4.07 RR43 pKa = 11.84 KK44 pKa = 7.64 QAKK47 pKa = 9.44 VSHH50 pKa = 6.43 LPFRR54 pKa = 11.84 MLSALMIAYY63 pKa = 9.39 SLYY66 pKa = 10.1 SVRR69 pKa = 11.84 PHH71 pKa = 6.12 QPIPMYY77 pKa = 10.48 RR78 pKa = 11.84 KK79 pKa = 9.55 PKK81 pKa = 10.04 FLNTNPEE88 pKa = 3.83 RR89 pKa = 11.84 KK90 pKa = 9.24 RR91 pKa = 11.84 RR92 pKa = 11.84 LLVYY96 pKa = 9.67 KK97 pKa = 10.48 KK98 pKa = 10.04 CDD100 pKa = 3.16 NSLCSRR106 pKa = 11.84 DD107 pKa = 3.53 HH108 pKa = 6.98 YY109 pKa = 10.7 EE110 pKa = 3.85 LQRR113 pKa = 11.84 SVVSRR118 pKa = 11.84 TRR120 pKa = 11.84 SNPITDD126 pKa = 4.57 HH127 pKa = 7.18 VNMMQTKK134 pKa = 9.28 SSEE137 pKa = 4.25 MFKK140 pKa = 9.56 KK141 pKa = 8.35 TQKK144 pKa = 10.48 QKK146 pKa = 9.97 WATEE150 pKa = 4.37 DD151 pKa = 3.46 EE152 pKa = 5.01 CSGPPHH158 pKa = 7.22 EE159 pKa = 4.53 IHH161 pKa = 7.0 IVQQNRR167 pKa = 11.84 GRR169 pKa = 11.84 LPSQGYY175 pKa = 8.86 VPVRR179 pKa = 11.84 NLLGEE184 pKa = 4.39 KK185 pKa = 10.3 NDD187 pKa = 3.43 ISTIDD192 pKa = 4.11 DD193 pKa = 3.56 LTYY196 pKa = 10.48 IPWWFLDD203 pKa = 3.83 DD204 pKa = 4.79 PEE206 pKa = 4.41 VTANADD212 pKa = 3.45 LTTSITYY219 pKa = 9.49 LPWATPLFDD228 pKa = 3.53 RR229 pKa = 11.84 CQRR232 pKa = 11.84 VAEE235 pKa = 4.25 WNTDD239 pKa = 2.89 AFKK242 pKa = 11.34 AEE244 pKa = 3.72 DD245 pKa = 3.4 VYY247 pKa = 11.64 YY248 pKa = 10.74 YY249 pKa = 10.72 EE250 pKa = 4.46 NSEE253 pKa = 4.03 KK254 pKa = 10.74 FMTTGIFEE262 pKa = 4.43 KK263 pKa = 10.51 SRR265 pKa = 11.84 QFRR268 pKa = 11.84 TRR270 pKa = 11.84 QLNLNATVVPQEE282 pKa = 5.06 KK283 pKa = 9.49 IDD285 pKa = 3.88 FLQTTLSQKK294 pKa = 9.39 FLCKK298 pKa = 10.12 HH299 pKa = 5.51 YY300 pKa = 10.54 LPCAKK305 pKa = 10.28 HH306 pKa = 6.28 EE307 pKa = 4.15 NTTNFDD313 pKa = 3.79 CPDD316 pKa = 3.5 CFACLRR322 pKa = 11.84 QTTLKK327 pKa = 10.53 CFLYY331 pKa = 10.54 EE332 pKa = 3.99 RR333 pKa = 11.84 AHH335 pKa = 6.34 IPAIRR340 pKa = 11.84 LRR342 pKa = 11.84 EE343 pKa = 4.17 GYY345 pKa = 9.87 FFNAHH350 pKa = 5.93 YY351 pKa = 10.94 LNDD354 pKa = 3.73 GPVFVNDD361 pKa = 3.46 QWWTFPMVHH370 pKa = 5.95 SHH372 pKa = 5.76 IQAGDD377 pKa = 3.35 TAFIPSVNSNYY388 pKa = 9.57 PSVYY392 pKa = 10.17 EE393 pKa = 4.11 VTSDD397 pKa = 4.25 LDD399 pKa = 3.72 EE400 pKa = 4.21 QAKK403 pKa = 10.26 LSYY406 pKa = 10.62 VGLRR410 pKa = 11.84 KK411 pKa = 7.9 TRR413 pKa = 11.84 KK414 pKa = 7.14 TLLIHH419 pKa = 6.23 PEE421 pKa = 3.97 KK422 pKa = 10.82 DD423 pKa = 2.93 VDD425 pKa = 3.79 MLICAGGKK433 pKa = 7.31 RR434 pKa = 11.84 TKK436 pKa = 10.92 EE437 pKa = 3.95 MIISLTKK444 pKa = 10.17 SDD446 pKa = 3.79 VVKK449 pKa = 10.97 GKK451 pKa = 9.95 LVPISQPGDD460 pKa = 3.42 CGSILRR466 pKa = 11.84 QKK468 pKa = 10.09 IEE470 pKa = 3.93 KK471 pKa = 10.15 DD472 pKa = 3.48 EE473 pKa = 4.32 PPKK476 pKa = 11.04 VVGIYY481 pKa = 9.56 TGEE484 pKa = 3.72 INMANSWMQVFSPLNPRR501 pKa = 11.84 VYY503 pKa = 10.38 RR504 pKa = 11.84 ILRR507 pKa = 11.84 NSRR510 pKa = 11.84 SAPLSIHH517 pKa = 6.12 HH518 pKa = 5.54 WHH520 pKa = 6.92 HH521 pKa = 6.33 YY522 pKa = 7.85 KK523 pKa = 9.7 TVALRR528 pKa = 11.84 QGLYY532 pKa = 10.38 KK533 pKa = 9.82 DD534 pKa = 3.44 TKK536 pKa = 10.63 EE537 pKa = 4.19 SIKK540 pKa = 10.46 NVKK543 pKa = 10.24 DD544 pKa = 2.83 ITEE547 pKa = 4.16 QIKK550 pKa = 10.5 KK551 pKa = 10.75 SNVTQHH557 pKa = 6.28 FADD560 pKa = 3.67 TAKK563 pKa = 10.0 TVNKK567 pKa = 9.84 VMTPASHH574 pKa = 7.09 ANNIAPISVILQLIAIAKK592 pKa = 9.39 AKK594 pKa = 8.02 TASAFFTAVIVLLGLLGVEE613 pKa = 4.0 DD614 pKa = 4.21 TMWRR618 pKa = 11.84 SVIKK622 pKa = 10.22 QFQVDD627 pKa = 3.78 YY628 pKa = 10.6 QNQGFSDD635 pKa = 4.16 LMQDD639 pKa = 3.85 LFPVVKK645 pKa = 10.22 ILDD648 pKa = 3.69 EE649 pKa = 5.03 FIPNLSFSSAIIKK662 pKa = 9.09 TGTYY666 pKa = 7.81 LTAYY670 pKa = 10.01 SKK672 pKa = 11.45 VKK674 pKa = 10.74 DD675 pKa = 3.53 HH676 pKa = 5.88 VLKK679 pKa = 10.88 VFHH682 pKa = 6.9 KK683 pKa = 10.01 HH684 pKa = 6.93 GIWQTEE690 pKa = 3.85 NYY692 pKa = 9.45 ILQDD696 pKa = 3.45 HH697 pKa = 6.6 AKK699 pKa = 9.69 EE700 pKa = 4.23 IQDD703 pKa = 3.59 HH704 pKa = 5.81 LVEE707 pKa = 5.07 KK708 pKa = 9.72 VPRR711 pKa = 11.84 WAAMSIEE718 pKa = 5.04 DD719 pKa = 4.05 LNKK722 pKa = 9.6 MNSEE726 pKa = 3.8 VDD728 pKa = 3.36 EE729 pKa = 4.4 MEE731 pKa = 4.12 NALVDD736 pKa = 4.18 LRR738 pKa = 11.84 AIQQFASGKK747 pKa = 9.99 KK748 pKa = 9.35 SLPLFGDD755 pKa = 3.98 ISWMRR760 pKa = 11.84 AVEE763 pKa = 4.33 SNIKK767 pKa = 8.7 TLEE770 pKa = 4.04 NLLKK774 pKa = 10.55 LVRR777 pKa = 11.84 TSRR780 pKa = 11.84 GSACTRR786 pKa = 11.84 QTPVALHH793 pKa = 6.47 IFGDD797 pKa = 3.69 SHH799 pKa = 7.77 IGKK802 pKa = 7.09 TTVTTYY808 pKa = 10.73 LVKK811 pKa = 10.76 QIVKK815 pKa = 10.24 DD816 pKa = 3.8 VEE818 pKa = 3.93 KK819 pKa = 10.85 LIINSYY825 pKa = 8.65 PGKK828 pKa = 10.53 SSAEE832 pKa = 3.87 LKK834 pKa = 10.62 RR835 pKa = 11.84 ILEE838 pKa = 4.08 SDD840 pKa = 3.29 EE841 pKa = 4.55 YY842 pKa = 11.39 EE843 pKa = 3.87 WFRR846 pKa = 11.84 DD847 pKa = 3.62 LKK849 pKa = 10.44 TSSTWSKK856 pKa = 11.4 NMSDD860 pKa = 4.34 EE861 pKa = 4.11 YY862 pKa = 10.82 DD863 pKa = 3.06 TGYY866 pKa = 10.66 VGQRR870 pKa = 11.84 VAIADD875 pKa = 3.62 EE876 pKa = 4.17 VFQDD880 pKa = 5.01 KK881 pKa = 8.23 EE882 pKa = 4.49 HH883 pKa = 6.77 KK884 pKa = 9.89 DD885 pKa = 3.31 HH886 pKa = 7.67 RR887 pKa = 11.84 IFLSWISPNPYY898 pKa = 8.74 LHH900 pKa = 6.45 TMASVEE906 pKa = 4.16 AKK908 pKa = 10.05 GYY910 pKa = 8.82 PDD912 pKa = 3.35 RR913 pKa = 11.84 TILIITTGNTAVPSHH928 pKa = 6.3 STTIAYY934 pKa = 8.58 PDD936 pKa = 3.71 ALHH939 pKa = 6.27 NRR941 pKa = 11.84 FVTWHH946 pKa = 6.96 PSNTLPVGQYY956 pKa = 8.89 PPPRR960 pKa = 11.84 MEE962 pKa = 5.24 DD963 pKa = 3.02 GKK965 pKa = 10.54 EE966 pKa = 3.85 VHH968 pKa = 6.8 SFDD971 pKa = 4.03 HH972 pKa = 6.95 LKK974 pKa = 9.52 GTLSYY979 pKa = 11.26 KK980 pKa = 9.82 SFKK983 pKa = 10.25 RR984 pKa = 11.84 DD985 pKa = 2.74 AKK987 pKa = 8.7 VQHH990 pKa = 5.25 VSKK993 pKa = 10.81 YY994 pKa = 8.71 IADD997 pKa = 4.8 RR998 pKa = 11.84 IMKK1001 pKa = 9.28 EE1002 pKa = 3.39 RR1003 pKa = 11.84 QTYY1006 pKa = 9.6 LRR1008 pKa = 11.84 SNQSEE1013 pKa = 4.64 VKK1015 pKa = 8.81 TATRR1019 pKa = 11.84 NGASFVEE1026 pKa = 4.47 LLEE1029 pKa = 4.39 QVKK1032 pKa = 10.44 QNQTTINTTRR1042 pKa = 11.84 LWEE1045 pKa = 4.05 SAKK1048 pKa = 10.34 KK1049 pKa = 10.28 KK1050 pKa = 10.74 KK1051 pKa = 10.35 EE1052 pKa = 3.7 FLEE1055 pKa = 4.09 KK1056 pKa = 10.17 HH1057 pKa = 5.7 LKK1059 pKa = 9.38 EE1060 pKa = 4.12 AVDD1063 pKa = 5.09 FISQNEE1069 pKa = 4.15 EE1070 pKa = 3.65 EE1071 pKa = 4.54 TAVPATLQVKK1081 pKa = 10.09 LEE1083 pKa = 4.04 HH1084 pKa = 6.66 RR1085 pKa = 11.84 LSEE1088 pKa = 4.4 TEE1090 pKa = 3.49 IHH1092 pKa = 6.63 NLLDD1096 pKa = 3.58 QIGIADD1102 pKa = 4.81 EE1103 pKa = 4.32 IDD1105 pKa = 3.33 HH1106 pKa = 6.69 KK1107 pKa = 11.09 IVVSMMKK1114 pKa = 10.5 GEE1116 pKa = 4.87 LEE1118 pKa = 4.53 MIPQGILEE1126 pKa = 4.39 HH1127 pKa = 5.35 TVKK1130 pKa = 10.48 IEE1132 pKa = 3.7 GDD1134 pKa = 3.3 KK1135 pKa = 11.24 VRR1137 pKa = 11.84 FTINKK1142 pKa = 8.47 NLCNSQTGDD1151 pKa = 3.88 LYY1153 pKa = 11.17 LQSGSLQASFQNFCNILSFISTEE1176 pKa = 4.03 RR1177 pKa = 11.84 VDD1179 pKa = 3.1 IEE1181 pKa = 3.96 YY1182 pKa = 10.67 RR1183 pKa = 11.84 EE1184 pKa = 4.49 WKK1186 pKa = 10.53 ASTGPGGAVFTRR1198 pKa = 11.84 DD1199 pKa = 3.37 YY1200 pKa = 10.16 TISDD1204 pKa = 3.88 TLPWLAQKK1212 pKa = 9.79 MKK1214 pKa = 10.42 LPEE1217 pKa = 4.33 SVTTALKK1224 pKa = 10.42 KK1225 pKa = 10.58 AVTAVEE1231 pKa = 4.09 QNKK1234 pKa = 10.29 SKK1236 pKa = 11.18 VIMAIFGVVFLIILGGFMKK1255 pKa = 10.35 IGLTANQRR1263 pKa = 11.84 RR1264 pKa = 11.84 EE1265 pKa = 3.92 MRR1267 pKa = 11.84 YY1268 pKa = 7.95 SCQSNRR1274 pKa = 11.84 QMTPQEE1280 pKa = 3.78 NQAAYY1285 pKa = 9.62 DD1286 pKa = 3.55 WSVMKK1291 pKa = 10.81 GADD1294 pKa = 3.2 RR1295 pKa = 11.84 HH1296 pKa = 5.62 LACAALPSEE1305 pKa = 4.75 EE1306 pKa = 5.04 FCTLPCVACEE1316 pKa = 3.99 TASKK1320 pKa = 10.2 IAVAHH1325 pKa = 5.97 KK1326 pKa = 10.76 VSTQKK1331 pKa = 10.65 EE1332 pKa = 3.97 KK1333 pKa = 11.22 VMILRR1338 pKa = 11.84 VVQSQSPEE1346 pKa = 3.65 NMEE1349 pKa = 3.84 NTKK1352 pKa = 9.99 ILPFVGIEE1360 pKa = 4.06 IPDD1363 pKa = 3.59 QSRR1366 pKa = 11.84 LLAANLAVEE1375 pKa = 4.03 TDD1377 pKa = 3.41 GFVFEE1382 pKa = 5.12 KK1383 pKa = 9.92 KK1384 pKa = 8.73 TFSVQEE1390 pKa = 4.07 DD1391 pKa = 3.47 QEE1393 pKa = 4.17 IADD1396 pKa = 4.37 FSNIATMISNEE1407 pKa = 3.91 SSAALQKK1414 pKa = 11.11 NKK1416 pKa = 9.91 VIHH1419 pKa = 6.47 SEE1421 pKa = 4.01 TSAYY1425 pKa = 8.45 TIPQKK1430 pKa = 10.75 RR1431 pKa = 11.84 ITSEE1435 pKa = 3.64 NSATIQKK1442 pKa = 10.56 GKK1444 pKa = 9.77 ILKK1447 pKa = 9.82 SEE1449 pKa = 4.24 SSAQVGKK1456 pKa = 10.94 GKK1458 pKa = 10.84 VIVSEE1463 pKa = 4.38 SSANIQHH1470 pKa = 6.61 GKK1472 pKa = 8.94 QIRR1475 pKa = 11.84 SEE1477 pKa = 4.2 SSAQLSKK1484 pKa = 10.54 RR1485 pKa = 11.84 QVYY1488 pKa = 10.04 HH1489 pKa = 7.36 SEE1491 pKa = 3.89 FTHH1494 pKa = 5.66 TNRR1497 pKa = 11.84 THH1499 pKa = 6.4 SPLVAYY1505 pKa = 8.39 TYY1507 pKa = 11.24 GMEE1510 pKa = 4.18 FTGEE1514 pKa = 3.78 EE1515 pKa = 4.36 EE1516 pKa = 3.95 IPVRR1520 pKa = 11.84 EE1521 pKa = 3.97 KK1522 pKa = 11.24 KK1523 pKa = 9.9 EE1524 pKa = 4.12 GKK1526 pKa = 9.82 EE1527 pKa = 3.86 EE1528 pKa = 3.99 MVEE1531 pKa = 4.05 RR1532 pKa = 11.84 TRR1534 pKa = 11.84 LEE1536 pKa = 4.07 TLLGMLRR1543 pKa = 11.84 SKK1545 pKa = 11.02 DD1546 pKa = 3.16 IGKK1549 pKa = 9.64 EE1550 pKa = 3.85 LMLVNALHH1558 pKa = 5.65 YY1559 pKa = 9.14 MGYY1562 pKa = 9.97 VPEE1565 pKa = 4.11 FHH1567 pKa = 7.14 PRR1569 pKa = 11.84 MSLDD1573 pKa = 3.2 HH1574 pKa = 6.55 FSSVVLRR1581 pKa = 11.84 LVGSQGTTQRR1591 pKa = 11.84 CEE1593 pKa = 3.22 ASYY1596 pKa = 11.69 AFVKK1600 pKa = 10.73 ADD1602 pKa = 3.6 ALLEE1606 pKa = 4.16 KK1607 pKa = 10.71 QLVNKK1612 pKa = 9.66 FRR1614 pKa = 11.84 DD1615 pKa = 3.91 LCWGEE1620 pKa = 4.61 SMCQFCVDD1628 pKa = 4.4 NIEE1631 pKa = 4.33 EE1632 pKa = 4.77 DD1633 pKa = 4.71 DD1634 pKa = 4.19 LLKK1637 pKa = 10.5 MNPLLHH1643 pKa = 6.33 IYY1645 pKa = 10.52 KK1646 pKa = 10.42 RR1647 pKa = 11.84 MSDD1650 pKa = 2.97 KK1651 pKa = 10.65 TLNSEE1656 pKa = 4.04 LLEE1659 pKa = 5.49 GGMHH1663 pKa = 7.38 LPGKK1667 pKa = 9.61 EE1668 pKa = 3.69 FAGPGTKK1675 pKa = 9.91 NAEE1678 pKa = 3.83 KK1679 pKa = 10.09 MMSFVAPTNNLDD1691 pKa = 3.75 SLSRR1695 pKa = 11.84 THH1697 pKa = 7.24 DD1698 pKa = 3.12 MKK1700 pKa = 11.39 VADD1703 pKa = 4.82 KK1704 pKa = 11.64 GEE1706 pKa = 4.53 LYY1708 pKa = 11.13 DD1709 pKa = 5.53 NDD1711 pKa = 4.14 DD1712 pKa = 3.42 KK1713 pKa = 11.72 QAFKK1717 pKa = 10.88 DD1718 pKa = 3.65 SYY1720 pKa = 10.5 IARR1723 pKa = 11.84 TSLLAAQKK1731 pKa = 9.95 FKK1733 pKa = 11.01 GWVGLLSGKK1742 pKa = 8.22 TEE1744 pKa = 4.16 VIKK1747 pKa = 11.09 NEE1749 pKa = 3.81 SGIPRR1754 pKa = 11.84 KK1755 pKa = 8.86 VTMFEE1760 pKa = 3.88 KK1761 pKa = 10.59 RR1762 pKa = 11.84 SDD1764 pKa = 3.63 LEE1766 pKa = 4.09 RR1767 pKa = 11.84 QFVTEE1772 pKa = 4.7 ACADD1776 pKa = 3.83 TNALSLKK1783 pKa = 10.3 RR1784 pKa = 11.84 KK1785 pKa = 9.63 VIEE1788 pKa = 4.19 KK1789 pKa = 10.43 NLVKK1793 pKa = 10.46 VWLYY1797 pKa = 11.2 EE1798 pKa = 3.87 GTTKK1802 pKa = 10.95 YY1803 pKa = 10.29 GVHH1806 pKa = 7.13 GIGSGNRR1813 pKa = 11.84 IYY1815 pKa = 10.51 TVAHH1819 pKa = 5.4 VFEE1822 pKa = 5.17 EE1823 pKa = 5.05 GSGTVVVEE1831 pKa = 4.56 TSSKK1835 pKa = 9.97 TYY1837 pKa = 9.18 TLPLSTVKK1845 pKa = 10.75 AHH1847 pKa = 6.52 LTQDD1851 pKa = 3.61 LAVIDD1856 pKa = 3.9 MSGVPKK1862 pKa = 8.95 FTPFANIDD1870 pKa = 3.5 KK1871 pKa = 10.73 QIASEE1876 pKa = 4.19 NDD1878 pKa = 3.24 LQQVTSIPLVSQYY1891 pKa = 10.28 FPSIQDD1897 pKa = 3.25 SFIGTKK1903 pKa = 7.57 EE1904 pKa = 3.86 EE1905 pKa = 4.09 KK1906 pKa = 10.16 LQKK1909 pKa = 9.19 LTNSSHH1915 pKa = 6.15 RR1916 pKa = 11.84 QIEE1919 pKa = 4.34 DD1920 pKa = 3.38 VFVVSTCAVSGMPSSDD1936 pKa = 3.58 GDD1938 pKa = 3.81 CGSPLFILSNNVQRR1952 pKa = 11.84 KK1953 pKa = 9.43 IIGLHH1958 pKa = 4.9 VGYY1961 pKa = 10.36 DD1962 pKa = 3.34 VRR1964 pKa = 11.84 TARR1967 pKa = 11.84 SMSVLITIEE1976 pKa = 3.86 KK1977 pKa = 9.58 LNRR1980 pKa = 11.84 IAAGKK1985 pKa = 9.98 DD1986 pKa = 3.58 YY1987 pKa = 11.0 QNHH1990 pKa = 6.21 NSTITEE1996 pKa = 4.05 ISRR1999 pKa = 11.84 TDD2001 pKa = 2.97 ILEE2004 pKa = 4.01 ILKK2007 pKa = 10.51 DD2008 pKa = 3.81 EE2009 pKa = 4.47 PAEE2012 pKa = 4.47 KK2013 pKa = 9.83 IPEE2016 pKa = 4.13 KK2017 pKa = 10.31 YY2018 pKa = 10.08 RR2019 pKa = 11.84 PVGKK2023 pKa = 10.21 FNNLTKK2029 pKa = 10.09 PVQSTRR2035 pKa = 11.84 MRR2037 pKa = 11.84 RR2038 pKa = 11.84 SIFHH2042 pKa = 6.81 RR2043 pKa = 11.84 KK2044 pKa = 8.71 FPEE2047 pKa = 4.03 VNRR2050 pKa = 11.84 KK2051 pKa = 8.25 PAALTSEE2058 pKa = 4.27 KK2059 pKa = 11.04 YY2060 pKa = 9.71 EE2061 pKa = 4.47 GEE2063 pKa = 4.07 MEE2065 pKa = 3.9 KK2066 pKa = 10.98 DD2067 pKa = 3.39 KK2068 pKa = 11.53 NGKK2071 pKa = 8.06 PDD2073 pKa = 4.1 LMITNISPWFKK2084 pKa = 10.01 KK2085 pKa = 9.17 TPLLSAEE2092 pKa = 5.22 DD2093 pKa = 3.8 KK2094 pKa = 10.74 NQLKK2098 pKa = 10.53 IITSNFYY2105 pKa = 9.83 NAQFKK2110 pKa = 10.37 TSMLTRR2116 pKa = 11.84 QKK2118 pKa = 11.32 NPLTEE2123 pKa = 4.27 KK2124 pKa = 10.49 EE2125 pKa = 4.2 ILNGTEE2131 pKa = 4.14 TMPGMNMQSGSGAYY2145 pKa = 8.35 WNRR2148 pKa = 11.84 FHH2150 pKa = 7.28 SKK2152 pKa = 10.24 KK2153 pKa = 10.11 KK2154 pKa = 10.49 GYY2156 pKa = 8.29 CQWVPEE2162 pKa = 4.38 GNQGYY2167 pKa = 8.0 WKK2169 pKa = 10.55 LADD2172 pKa = 3.47 HH2173 pKa = 6.72 QAAQKK2178 pKa = 10.48 LKK2180 pKa = 10.64 AYY2182 pKa = 10.39 LKK2184 pKa = 10.6 LKK2186 pKa = 9.8 EE2187 pKa = 4.02 EE2188 pKa = 4.21 KK2189 pKa = 10.18 ALKK2192 pKa = 10.65 GEE2194 pKa = 4.21 EE2195 pKa = 4.17 LLEE2198 pKa = 4.76 LATTHH2203 pKa = 7.01 LKK2205 pKa = 10.73 DD2206 pKa = 3.29 EE2207 pKa = 4.48 CRR2209 pKa = 11.84 KK2210 pKa = 7.9 VTNGVRR2216 pKa = 11.84 KK2217 pKa = 7.95 LTRR2220 pKa = 11.84 MYY2222 pKa = 10.88 VCNSLPAFMHH2232 pKa = 5.8 EE2233 pKa = 4.35 KK2234 pKa = 10.31 KK2235 pKa = 10.65 FLGRR2239 pKa = 11.84 FVEE2242 pKa = 4.79 YY2243 pKa = 10.65 FFDD2246 pKa = 3.39 NNYY2249 pKa = 10.77 AIGHH2253 pKa = 5.89 GVTVNPLSKK2262 pKa = 10.18 SWGVLYY2268 pKa = 10.53 EE2269 pKa = 4.14 KK2270 pKa = 10.61 LLKK2273 pKa = 10.48 KK2274 pKa = 10.46 GSNWFDD2280 pKa = 3.29 GDD2282 pKa = 3.49 FKK2284 pKa = 11.53 NYY2286 pKa = 10.11 DD2287 pKa = 3.62 RR2288 pKa = 11.84 YY2289 pKa = 8.07 QHH2291 pKa = 6.91 RR2292 pKa = 11.84 DD2293 pKa = 3.89 FISAAWDD2300 pKa = 3.15 IVNRR2304 pKa = 11.84 WTLEE2308 pKa = 3.47 NLRR2311 pKa = 11.84 YY2312 pKa = 9.66 KK2313 pKa = 10.8 NKK2315 pKa = 10.56 AGLSMLLNGCKK2326 pKa = 9.31 EE2327 pKa = 3.71 QFIYY2331 pKa = 9.31 STHH2334 pKa = 6.99 AYY2336 pKa = 10.43 DD2337 pKa = 4.31 NDD2339 pKa = 3.95 VFLTDD2344 pKa = 3.69 HH2345 pKa = 6.82 GNPSGHH2351 pKa = 6.61 TLTTVINCIANLLYY2365 pKa = 10.74 HH2366 pKa = 6.86 RR2367 pKa = 11.84 FLYY2370 pKa = 10.26 HH2371 pKa = 6.82 KK2372 pKa = 10.56 LVGTSAEE2379 pKa = 4.05 NTEE2382 pKa = 4.65 KK2383 pKa = 10.78 EE2384 pKa = 4.1 FEE2386 pKa = 4.25 KK2387 pKa = 11.04 NVAIFVFGDD2396 pKa = 3.51 DD2397 pKa = 4.15 VIISVSDD2404 pKa = 3.51 AVKK2407 pKa = 10.15 EE2408 pKa = 4.26 VYY2410 pKa = 10.26 NLEE2413 pKa = 4.21 TLSVAFSCLGQIYY2426 pKa = 8.81 TSGAKK2431 pKa = 9.67 DD2432 pKa = 3.01 GKK2434 pKa = 9.23 MYY2436 pKa = 10.35 RR2437 pKa = 11.84 YY2438 pKa = 9.82 RR2439 pKa = 11.84 NPDD2442 pKa = 2.69 EE2443 pKa = 4.01 ATFLKK2448 pKa = 10.45 RR2449 pKa = 11.84 RR2450 pKa = 11.84 SFKK2453 pKa = 10.64 LKK2455 pKa = 10.51 YY2456 pKa = 9.27 MVVAQLEE2463 pKa = 4.4 WDD2465 pKa = 4.17 SIISPFSWTKK2475 pKa = 11.1 LDD2477 pKa = 4.96 DD2478 pKa = 3.85 SDD2480 pKa = 4.49 KK2481 pKa = 10.52 NGYY2484 pKa = 8.46 VAMVEE2489 pKa = 4.04 SCLIEE2494 pKa = 4.57 ACLHH2498 pKa = 4.79 GRR2500 pKa = 11.84 QRR2502 pKa = 11.84 YY2503 pKa = 8.9 EE2504 pKa = 3.85 EE2505 pKa = 4.18 LSGKK2509 pKa = 9.91 IKK2511 pKa = 10.72 DD2512 pKa = 3.41 IMKK2515 pKa = 10.45 EE2516 pKa = 3.76 KK2517 pKa = 10.47 FNYY2520 pKa = 9.67 DD2521 pKa = 2.79 IPSFGEE2527 pKa = 3.94 AYY2529 pKa = 9.28 LTFKK2533 pKa = 10.29 KK2534 pKa = 10.22 QYY2536 pKa = 9.58 HH2537 pKa = 5.53 AA2538 pKa = 4.86
Molecular weight: 289.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.569
IPC2_protein 7.922
IPC_protein 7.702
Toseland 7.702
ProMoST 8.331
Dawson 8.595
Bjellqvist 8.741
Wikipedia 8.58
Rodwell 8.682
Grimsley 7.819
Solomon 8.609
Lehninger 8.609
Nozaki 8.887
DTASelect 8.551
Thurlkill 8.653
EMBOSS 8.77
Sillero 8.887
Patrickios 4.52
IPC_peptide 8.609
IPC2_peptide 7.439
IPC2.peptide.svr19 7.776
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
3371
833
2538
1685.5
190.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.437 ± 0.798
1.246 ± 0.202
5.102 ± 0.149
6.408 ± 0.5
4.301 ± 0.05
5.31 ± 0.762
2.877 ± 0.058
5.696 ± 0.325
7.802 ± 0.954
8.781 ± 0.17
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.611 ± 0.044
4.539 ± 0.466
4.301 ± 0.607
4.301 ± 0.01
4.034 ± 0.633
7.891 ± 0.434
6.912 ± 0.383
6.704 ± 0.128
1.276 ± 0.097
3.471 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here