Aquilegia coerulea (Rocky mountain columbine)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Ranunculales; Ranunculaceae; Thalictroideae; Aquilegia

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37018 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G5FB02|A0A2G5FB02_AQUCA Uncharacterized protein OS=Aquilegia coerulea OX=218851 GN=AQUCO_00100556v1 PE=4 SV=1
MM1 pKa = 7.57SKK3 pKa = 10.21KK4 pKa = 8.31QTEE7 pKa = 4.27KK8 pKa = 11.08YY9 pKa = 9.88IFRR12 pKa = 11.84DD13 pKa = 3.26EE14 pKa = 4.46CDD16 pKa = 2.91YY17 pKa = 11.43DD18 pKa = 4.44YY19 pKa = 11.64YY20 pKa = 11.55FKK22 pKa = 11.07FSDD25 pKa = 3.98DD26 pKa = 4.3DD27 pKa = 4.41FYY29 pKa = 11.65STNEE33 pKa = 3.84LPIKK37 pKa = 10.34EE38 pKa = 4.24EE39 pKa = 3.95TIEE42 pKa = 4.04AVMNLLEE49 pKa = 5.24KK50 pKa = 10.44EE51 pKa = 4.29LNDD54 pKa = 4.19VRR56 pKa = 11.84NASIKK61 pKa = 10.07MKK63 pKa = 7.72EE64 pKa = 4.2TCDD67 pKa = 3.24VTFSNEE73 pKa = 3.56SSTVMASFDD82 pKa = 4.41LNGLFNISYY91 pKa = 10.28LNDD94 pKa = 3.41SFPFQSVFYY103 pKa = 9.39TSSTSLPPPPPPPLPFTQLAKK124 pKa = 10.75SQLSWCSSVNNNNNQLCEE142 pKa = 4.04FEE144 pKa = 4.71ICSSSNVDD152 pKa = 3.6LVNGTEE158 pKa = 4.0QEE160 pKa = 4.2EE161 pKa = 4.47EE162 pKa = 4.14QVSGSDD168 pKa = 3.5EE169 pKa = 4.42TNGGCEE175 pKa = 3.84EE176 pKa = 4.57HH177 pKa = 6.92VVSTTDD183 pKa = 3.45LNEE186 pKa = 4.44DD187 pKa = 2.81EE188 pKa = 5.09WMDD191 pKa = 5.01LLTLDD196 pKa = 4.86GFSFDD201 pKa = 4.07LL202 pKa = 4.66

Molecular weight: 22.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G5EHG7|A0A2G5EHG7_AQUCA Protein kinase domain-containing protein OS=Aquilegia coerulea OX=218851 GN=AQUCO_00800146v1 PE=3 SV=1
MM1 pKa = 7.52AGARR5 pKa = 11.84RR6 pKa = 11.84STMMININGGSGNRR20 pKa = 11.84QRR22 pKa = 11.84LSSSRR27 pKa = 11.84LIPKK31 pKa = 9.91RR32 pKa = 11.84GQVKK36 pKa = 10.23AGIAIGLAHH45 pKa = 6.87SLSAIFSLNNNNNRR59 pKa = 11.84RR60 pKa = 11.84SSGSNLLAKK69 pKa = 10.09PSRR72 pKa = 11.84TIQQ75 pKa = 3.1

Molecular weight: 7.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24680

12338

37018

15996891

33

5451

432.1

48.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.251 ± 0.012

1.861 ± 0.006

5.32 ± 0.008

6.471 ± 0.012

4.254 ± 0.01

6.434 ± 0.013

2.385 ± 0.006

5.635 ± 0.01

6.212 ± 0.013

9.754 ± 0.014

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.459 ± 0.005

4.59 ± 0.007

4.682 ± 0.012

3.77 ± 0.01

5.104 ± 0.009

8.972 ± 0.015

5.055 ± 0.006

6.594 ± 0.009

1.267 ± 0.005

2.931 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski