Roseivivax pacificus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Allosediminivita

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4615 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T5ZWM0|A0A2T5ZWM0_9RHOB Type IV secretion system protein VirB3 OS=Roseivivax pacificus OX=1267769 GN=C8N44_1637 PE=4 SV=1
QQ1 pKa = 7.63PDD3 pKa = 3.43ADD5 pKa = 3.71AQQTVDD11 pKa = 4.54AFSEE15 pKa = 4.51AYY17 pKa = 9.88PNVEE21 pKa = 4.09VEE23 pKa = 4.23WVRR26 pKa = 11.84DD27 pKa = 3.46GTTQMMARR35 pKa = 11.84LRR37 pKa = 11.84AEE39 pKa = 4.42FEE41 pKa = 4.19AGQPQPDD48 pKa = 3.71VLLIADD54 pKa = 4.56TVTMEE59 pKa = 4.35SLEE62 pKa = 4.11QEE64 pKa = 4.58GRR66 pKa = 11.84LLAYY70 pKa = 9.92PEE72 pKa = 5.08ADD74 pKa = 2.82TAAYY78 pKa = 9.99QEE80 pKa = 4.49ALMDD84 pKa = 3.84PEE86 pKa = 5.43GYY88 pKa = 10.5YY89 pKa = 10.8FSTKK93 pKa = 10.38LITSGIVYY101 pKa = 8.06NTAAEE106 pKa = 4.26MVPSSYY112 pKa = 11.18EE113 pKa = 3.71DD114 pKa = 3.67LLAEE118 pKa = 4.13EE119 pKa = 4.41ATNNIVMPSPLEE131 pKa = 4.14SGAATIHH138 pKa = 5.55MVSLTGLPEE147 pKa = 4.68LGWNYY152 pKa = 10.39YY153 pKa = 10.01QGLADD158 pKa = 4.07QGAIAQGGNGGTYY171 pKa = 9.68RR172 pKa = 11.84AVAGGQALYY181 pKa = 10.97GFVVDD186 pKa = 7.14FLALRR191 pKa = 11.84NIQDD195 pKa = 3.68GAPVGFVFPEE205 pKa = 4.09EE206 pKa = 4.17GVSAVTEE213 pKa = 3.94PVAILEE219 pKa = 4.42SANNVPAAKK228 pKa = 10.3AFVDD232 pKa = 4.7FVISEE237 pKa = 4.22EE238 pKa = 4.23GQEE241 pKa = 3.75LAARR245 pKa = 11.84QGYY248 pKa = 9.37LPAHH252 pKa = 7.28PDD254 pKa = 3.08VAAPDD259 pKa = 3.91YY260 pKa = 10.94FPDD263 pKa = 3.58RR264 pKa = 11.84SEE266 pKa = 3.95ISVITFDD273 pKa = 4.29PAEE276 pKa = 4.13ALEE279 pKa = 4.34NDD281 pKa = 3.4AAYY284 pKa = 9.81KK285 pKa = 10.46EE286 pKa = 4.62RR287 pKa = 11.84FSEE290 pKa = 4.25MFGGG294 pKa = 3.99

Molecular weight: 31.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T6AX61|A0A2T6AX61_9RHOB Threonine/homoserine/homoserine lactone efflux protein OS=Roseivivax pacificus OX=1267769 GN=C8N44_109105 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNYY10 pKa = 8.43VRR12 pKa = 11.84KK13 pKa = 9.11HH14 pKa = 3.78RR15 pKa = 11.84HH16 pKa = 4.09GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.45AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4615

0

4615

1438208

26

2928

311.6

33.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.412 ± 0.053

0.862 ± 0.012

6.014 ± 0.031

6.447 ± 0.038

3.607 ± 0.022

8.937 ± 0.035

2.033 ± 0.015

4.791 ± 0.024

2.613 ± 0.032

10.196 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.749 ± 0.02

2.281 ± 0.02

5.312 ± 0.027

3.047 ± 0.022

7.187 ± 0.039

5.158 ± 0.023

5.458 ± 0.024

7.318 ± 0.03

1.427 ± 0.015

2.151 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski