Streptococcus phage CHPC927

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Moineauvirus; unclassified Moineauvirus

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8F7V5|A0A3G8F7V5_9CAUD Cro-like repressor OS=Streptococcus phage CHPC927 OX=2365048 GN=CHPC927_0029 PE=4 SV=1
MM1 pKa = 7.12VNWVDD6 pKa = 4.58KK7 pKa = 11.1DD8 pKa = 4.24GNDD11 pKa = 3.97LPDD14 pKa = 4.77GADD17 pKa = 3.13QDD19 pKa = 4.52FKK21 pKa = 11.38PGMFFSFAGDD31 pKa = 3.45EE32 pKa = 4.32VNITDD37 pKa = 3.99TGQGGYY43 pKa = 10.17YY44 pKa = 9.8GGYY47 pKa = 7.73YY48 pKa = 9.41YY49 pKa = 10.76RR50 pKa = 11.84RR51 pKa = 11.84FEE53 pKa = 4.06FGQFGTVWLSCWNKK67 pKa = 10.66DD68 pKa = 3.89DD69 pKa = 5.14LVNYY73 pKa = 7.38YY74 pKa = 8.26QQ75 pKa = 4.22

Molecular weight: 8.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8FAM3|A0A3G8FAM3_9CAUD Cro-like protein OS=Streptococcus phage CHPC927 OX=2365048 GN=CHPC927_0026 PE=4 SV=1
MM1 pKa = 7.56SKK3 pKa = 9.22TKK5 pKa = 10.5RR6 pKa = 11.84GVCANCHH13 pKa = 4.85TAFEE17 pKa = 4.44VSKK20 pKa = 10.32KK21 pKa = 7.01QRR23 pKa = 11.84RR24 pKa = 11.84KK25 pKa = 8.8IKK27 pKa = 8.88EE28 pKa = 4.09GKK30 pKa = 9.71SVFCSEE36 pKa = 4.16TCSLEE41 pKa = 4.41KK42 pKa = 10.69YY43 pKa = 9.93GKK45 pKa = 8.13TKK47 pKa = 9.25ITISEE52 pKa = 4.39IPCCRR57 pKa = 11.84CGKK60 pKa = 9.2MFTPTYY66 pKa = 9.91SQYY69 pKa = 11.19KK70 pKa = 7.81RR71 pKa = 11.84YY72 pKa = 9.99KK73 pKa = 8.85YY74 pKa = 10.7NDD76 pKa = 3.28YY77 pKa = 10.92VSNSFCSNEE86 pKa = 3.99CRR88 pKa = 11.84WKK90 pKa = 10.73KK91 pKa = 10.23EE92 pKa = 4.07YY93 pKa = 9.51PCKK96 pKa = 10.6YY97 pKa = 8.82HH98 pKa = 7.57DD99 pKa = 5.03DD100 pKa = 4.04YY101 pKa = 12.18VSVFVNGKK109 pKa = 9.91EE110 pKa = 3.7ILLDD114 pKa = 3.54FDD116 pKa = 4.38VFEE119 pKa = 5.31KK120 pKa = 10.89YY121 pKa = 10.71SKK123 pKa = 9.48TLYY126 pKa = 8.88VQNDD130 pKa = 2.67KK131 pKa = 10.98RR132 pKa = 11.84SNYY135 pKa = 8.34NSVMVFEE142 pKa = 4.8KK143 pKa = 10.65GKK145 pKa = 10.63KK146 pKa = 8.66ILSRR150 pKa = 11.84LIMSVADD157 pKa = 3.41KK158 pKa = 11.07NKK160 pKa = 10.77YY161 pKa = 8.8IDD163 pKa = 4.82HH164 pKa = 7.04INGNPLDD171 pKa = 3.85NRR173 pKa = 11.84RR174 pKa = 11.84SNLRR178 pKa = 11.84VVSHH182 pKa = 5.96QEE184 pKa = 3.05NMMNKK189 pKa = 8.25KK190 pKa = 8.41TYY192 pKa = 10.6KK193 pKa = 10.75NNTSKK198 pKa = 10.74IKK200 pKa = 10.53GVNLNKK206 pKa = 10.19KK207 pKa = 8.12GLWVARR213 pKa = 11.84IQVRR217 pKa = 11.84NQRR220 pKa = 11.84IFLGSSKK227 pKa = 10.56DD228 pKa = 3.26KK229 pKa = 11.18SVAEE233 pKa = 4.0RR234 pKa = 11.84LRR236 pKa = 11.84IEE238 pKa = 4.16AEE240 pKa = 3.57KK241 pKa = 10.62KK242 pKa = 10.51YY243 pKa = 10.45FGKK246 pKa = 10.56YY247 pKa = 8.89DD248 pKa = 3.6RR249 pKa = 11.84KK250 pKa = 10.04FLKK253 pKa = 10.59

Molecular weight: 29.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

11480

41

1621

239.2

27.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.411 ± 0.542

0.645 ± 0.128

6.551 ± 0.242

6.446 ± 0.518

4.399 ± 0.24

6.786 ± 0.51

1.429 ± 0.098

6.341 ± 0.21

8.467 ± 0.608

7.622 ± 0.428

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.465 ± 0.185

6.498 ± 0.317

3.04 ± 0.215

4.408 ± 0.222

4.016 ± 0.284

6.402 ± 0.3

6.559 ± 0.447

5.81 ± 0.252

1.437 ± 0.139

4.268 ± 0.327
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski