Amino acid dipepetide frequency for Streptococcus phage CHPC927

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.613AlaAla: 2.613 ± 0.953
0.174AlaCys: 0.174 ± 0.134
5.14AlaAsp: 5.14 ± 1.15
4.53AlaGlu: 4.53 ± 0.575
2.701AlaPhe: 2.701 ± 0.649
3.746AlaGly: 3.746 ± 0.717
0.784AlaHis: 0.784 ± 0.265
4.182AlaIle: 4.182 ± 0.785
5.575AlaLys: 5.575 ± 0.857
5.227AlaLeu: 5.227 ± 0.933
1.481AlaMet: 1.481 ± 0.382
4.791AlaAsn: 4.791 ± 0.976
1.655AlaPro: 1.655 ± 0.322
2.875AlaGln: 2.875 ± 0.532
2.701AlaArg: 2.701 ± 0.51
4.182AlaSer: 4.182 ± 0.62
3.92AlaThr: 3.92 ± 0.797
4.094AlaVal: 4.094 ± 0.569
1.045AlaTrp: 1.045 ± 0.245
2.701AlaTyr: 2.701 ± 0.734
0.0AlaXaa: 0.0 ± 0.0
Cys
0.261CysAla: 0.261 ± 0.128
0.087CysCys: 0.087 ± 0.085
0.784CysAsp: 0.784 ± 0.301
0.523CysGlu: 0.523 ± 0.261
0.436CysPhe: 0.436 ± 0.249
0.261CysGly: 0.261 ± 0.159
0.174CysHis: 0.174 ± 0.123
0.0CysIle: 0.0 ± 0.0
0.436CysLys: 0.436 ± 0.23
0.348CysLeu: 0.348 ± 0.191
0.087CysMet: 0.087 ± 0.086
0.261CysAsn: 0.261 ± 0.15
0.174CysPro: 0.174 ± 0.127
0.087CysGln: 0.087 ± 0.111
0.436CysArg: 0.436 ± 0.291
0.958CysSer: 0.958 ± 0.38
0.436CysThr: 0.436 ± 0.195
0.348CysVal: 0.348 ± 0.156
0.174CysTrp: 0.174 ± 0.12
0.174CysTyr: 0.174 ± 0.114
0.0CysXaa: 0.0 ± 0.0
Asp
3.746AspAla: 3.746 ± 0.511
0.523AspCys: 0.523 ± 0.278
4.617AspAsp: 4.617 ± 0.848
4.443AspGlu: 4.443 ± 0.788
4.094AspPhe: 4.094 ± 0.627
6.534AspGly: 6.534 ± 1.605
0.697AspHis: 0.697 ± 0.255
4.791AspIle: 4.791 ± 0.74
4.704AspLys: 4.704 ± 0.651
4.356AspLeu: 4.356 ± 0.789
2.526AspMet: 2.526 ± 0.482
4.53AspAsn: 4.53 ± 0.728
2.178AspPro: 2.178 ± 0.489
1.481AspGln: 1.481 ± 0.309
2.439AspArg: 2.439 ± 0.396
3.746AspSer: 3.746 ± 0.754
3.746AspThr: 3.746 ± 0.556
2.875AspVal: 2.875 ± 0.495
0.697AspTrp: 0.697 ± 0.278
2.788AspTyr: 2.788 ± 0.536
0.0AspXaa: 0.0 ± 0.0
Glu
4.007GluAla: 4.007 ± 0.589
0.436GluCys: 0.436 ± 0.176
3.746GluAsp: 3.746 ± 0.705
4.617GluGlu: 4.617 ± 0.857
2.004GluPhe: 2.004 ± 0.348
2.701GluGly: 2.701 ± 0.402
1.307GluHis: 1.307 ± 0.342
5.575GluIle: 5.575 ± 0.771
4.704GluLys: 4.704 ± 1.086
6.011GluLeu: 6.011 ± 0.9
2.178GluMet: 2.178 ± 0.515
3.485GluAsn: 3.485 ± 0.668
1.568GluPro: 1.568 ± 0.512
3.223GluGln: 3.223 ± 0.653
2.962GluArg: 2.962 ± 0.613
3.659GluSer: 3.659 ± 0.529
3.485GluThr: 3.485 ± 0.529
4.791GluVal: 4.791 ± 0.659
0.871GluTrp: 0.871 ± 0.285
3.136GluTyr: 3.136 ± 0.546
0.0GluXaa: 0.0 ± 0.0
Phe
3.049PheAla: 3.049 ± 0.525
0.261PheCys: 0.261 ± 0.18
3.746PheAsp: 3.746 ± 0.588
2.962PheGlu: 2.962 ± 0.732
1.742PhePhe: 1.742 ± 0.399
3.572PheGly: 3.572 ± 0.855
0.523PheHis: 0.523 ± 0.192
2.788PheIle: 2.788 ± 0.466
4.094PheLys: 4.094 ± 0.742
3.049PheLeu: 3.049 ± 0.485
0.436PheMet: 0.436 ± 0.203
3.92PheAsn: 3.92 ± 0.672
0.784PhePro: 0.784 ± 0.301
1.481PheGln: 1.481 ± 0.339
1.307PheArg: 1.307 ± 0.315
2.439PheSer: 2.439 ± 0.472
2.352PheThr: 2.352 ± 0.48
2.962PheVal: 2.962 ± 0.5
0.784PheTrp: 0.784 ± 0.252
1.742PheTyr: 1.742 ± 0.403
0.0PheXaa: 0.0 ± 0.0
Gly
3.049GlyAla: 3.049 ± 0.741
0.61GlyCys: 0.61 ± 0.253
3.746GlyAsp: 3.746 ± 0.532
3.398GlyGlu: 3.398 ± 0.507
3.485GlyPhe: 3.485 ± 0.613
3.92GlyGly: 3.92 ± 0.8
1.133GlyHis: 1.133 ± 0.395
4.966GlyIle: 4.966 ± 0.539
6.621GlyLys: 6.621 ± 0.896
6.098GlyLeu: 6.098 ± 0.733
1.742GlyMet: 1.742 ± 0.545
4.094GlyAsn: 4.094 ± 0.632
1.568GlyPro: 1.568 ± 0.572
3.136GlyGln: 3.136 ± 0.548
2.526GlyArg: 2.526 ± 0.451
3.833GlySer: 3.833 ± 0.777
5.053GlyThr: 5.053 ± 0.82
4.182GlyVal: 4.182 ± 0.768
1.394GlyTrp: 1.394 ± 0.35
3.31GlyTyr: 3.31 ± 0.464
0.0GlyXaa: 0.0 ± 0.0
His
0.61HisAla: 0.61 ± 0.241
0.174HisCys: 0.174 ± 0.118
0.784HisAsp: 0.784 ± 0.276
0.348HisGlu: 0.348 ± 0.238
0.697HisPhe: 0.697 ± 0.245
0.784HisGly: 0.784 ± 0.24
0.261HisHis: 0.261 ± 0.129
1.307HisIle: 1.307 ± 0.371
1.22HisLys: 1.22 ± 0.268
1.22HisLeu: 1.22 ± 0.261
0.348HisMet: 0.348 ± 0.232
0.958HisAsn: 0.958 ± 0.277
0.436HisPro: 0.436 ± 0.182
0.523HisGln: 0.523 ± 0.275
0.523HisArg: 0.523 ± 0.239
0.958HisSer: 0.958 ± 0.321
0.958HisThr: 0.958 ± 0.257
1.22HisVal: 1.22 ± 0.246
0.087HisTrp: 0.087 ± 0.088
0.871HisTyr: 0.871 ± 0.299
0.0HisXaa: 0.0 ± 0.0
Ile
4.443IleAla: 4.443 ± 0.824
0.436IleCys: 0.436 ± 0.217
4.617IleAsp: 4.617 ± 0.717
3.746IleGlu: 3.746 ± 0.627
1.742IlePhe: 1.742 ± 0.312
4.878IleGly: 4.878 ± 0.681
0.697IleHis: 0.697 ± 0.236
3.31IleIle: 3.31 ± 0.616
6.185IleLys: 6.185 ± 0.729
3.92IleLeu: 3.92 ± 0.614
1.917IleMet: 1.917 ± 0.455
3.92IleAsn: 3.92 ± 0.597
2.701IlePro: 2.701 ± 0.571
3.223IleGln: 3.223 ± 0.482
2.526IleArg: 2.526 ± 0.444
4.356IleSer: 4.356 ± 0.695
4.356IleThr: 4.356 ± 0.487
3.92IleVal: 3.92 ± 0.598
0.784IleTrp: 0.784 ± 0.224
1.742IleTyr: 1.742 ± 0.432
0.0IleXaa: 0.0 ± 0.0
Lys
5.663LysAla: 5.663 ± 0.631
0.348LysCys: 0.348 ± 0.25
4.966LysAsp: 4.966 ± 0.824
7.666LysGlu: 7.666 ± 1.177
3.398LysPhe: 3.398 ± 0.79
5.401LysGly: 5.401 ± 0.602
0.958LysHis: 0.958 ± 0.396
5.575LysIle: 5.575 ± 0.688
6.708LysLys: 6.708 ± 1.674
6.359LysLeu: 6.359 ± 0.875
2.439LysMet: 2.439 ± 0.624
5.575LysAsn: 5.575 ± 0.904
3.049LysPro: 3.049 ± 0.438
4.269LysGln: 4.269 ± 0.665
3.659LysArg: 3.659 ± 0.527
4.356LysSer: 4.356 ± 0.429
5.401LysThr: 5.401 ± 0.906
3.746LysVal: 3.746 ± 0.76
1.045LysTrp: 1.045 ± 0.274
4.094LysTyr: 4.094 ± 0.918
0.0LysXaa: 0.0 ± 0.0
Leu
6.098LeuAla: 6.098 ± 0.712
0.436LeuCys: 0.436 ± 0.216
5.488LeuAsp: 5.488 ± 0.722
5.488LeuGlu: 5.488 ± 0.902
2.962LeuPhe: 2.962 ± 0.405
5.924LeuGly: 5.924 ± 0.762
1.045LeuHis: 1.045 ± 0.31
3.485LeuIle: 3.485 ± 0.429
6.882LeuLys: 6.882 ± 0.767
5.227LeuLeu: 5.227 ± 0.846
2.265LeuMet: 2.265 ± 0.491
5.053LeuAsn: 5.053 ± 0.682
2.178LeuPro: 2.178 ± 0.337
2.091LeuGln: 2.091 ± 0.439
3.223LeuArg: 3.223 ± 0.745
5.401LeuSer: 5.401 ± 0.986
5.924LeuThr: 5.924 ± 0.961
4.007LeuVal: 4.007 ± 0.536
0.697LeuTrp: 0.697 ± 0.203
2.265LeuTyr: 2.265 ± 0.462
0.0LeuXaa: 0.0 ± 0.0
Met
1.655MetAla: 1.655 ± 0.431
0.0MetCys: 0.0 ± 0.0
1.045MetAsp: 1.045 ± 0.258
1.22MetGlu: 1.22 ± 0.464
1.307MetPhe: 1.307 ± 0.274
1.22MetGly: 1.22 ± 0.315
0.348MetHis: 0.348 ± 0.196
1.394MetIle: 1.394 ± 0.39
2.962MetLys: 2.962 ± 0.698
1.829MetLeu: 1.829 ± 0.348
0.697MetMet: 0.697 ± 0.216
1.133MetAsn: 1.133 ± 0.318
0.958MetPro: 0.958 ± 0.255
0.871MetGln: 0.871 ± 0.285
0.61MetArg: 0.61 ± 0.201
2.352MetSer: 2.352 ± 0.411
1.917MetThr: 1.917 ± 0.397
1.655MetVal: 1.655 ± 0.439
0.174MetTrp: 0.174 ± 0.108
1.307MetTyr: 1.307 ± 0.462
0.0MetXaa: 0.0 ± 0.0
Asn
5.227AsnAla: 5.227 ± 0.967
0.261AsnCys: 0.261 ± 0.151
3.136AsnAsp: 3.136 ± 0.55
3.572AsnGlu: 3.572 ± 0.754
3.31AsnPhe: 3.31 ± 0.603
6.621AsnGly: 6.621 ± 1.193
0.871AsnHis: 0.871 ± 0.222
3.659AsnIle: 3.659 ± 0.552
4.53AsnLys: 4.53 ± 0.552
5.227AsnLeu: 5.227 ± 0.539
1.481AsnMet: 1.481 ± 0.373
4.182AsnAsn: 4.182 ± 0.754
3.223AsnPro: 3.223 ± 0.714
3.136AsnGln: 3.136 ± 0.545
2.613AsnArg: 2.613 ± 0.566
4.182AsnSer: 4.182 ± 0.637
3.136AsnThr: 3.136 ± 0.457
3.746AsnVal: 3.746 ± 0.699
0.871AsnTrp: 0.871 ± 0.276
2.004AsnTyr: 2.004 ± 0.453
0.0AsnXaa: 0.0 ± 0.0
Pro
1.742ProAla: 1.742 ± 0.344
0.348ProCys: 0.348 ± 0.207
1.22ProAsp: 1.22 ± 0.343
1.829ProGlu: 1.829 ± 0.432
1.045ProPhe: 1.045 ± 0.287
1.394ProGly: 1.394 ± 0.566
0.261ProHis: 0.261 ± 0.172
1.307ProIle: 1.307 ± 0.294
4.007ProLys: 4.007 ± 0.432
2.352ProLeu: 2.352 ± 0.367
0.174ProMet: 0.174 ± 0.152
2.788ProAsn: 2.788 ± 0.372
0.348ProPro: 0.348 ± 0.189
1.655ProGln: 1.655 ± 0.305
1.394ProArg: 1.394 ± 0.468
3.136ProSer: 3.136 ± 0.557
2.352ProThr: 2.352 ± 0.474
1.133ProVal: 1.133 ± 0.396
0.61ProTrp: 0.61 ± 0.178
1.307ProTyr: 1.307 ± 0.369
0.0ProXaa: 0.0 ± 0.0
Gln
3.485GlnAla: 3.485 ± 0.619
0.174GlnCys: 0.174 ± 0.132
2.439GlnAsp: 2.439 ± 0.409
3.136GlnGlu: 3.136 ± 0.749
1.829GlnPhe: 1.829 ± 0.45
3.92GlnGly: 3.92 ± 0.781
0.436GlnHis: 0.436 ± 0.196
2.265GlnIle: 2.265 ± 0.441
3.31GlnLys: 3.31 ± 0.601
3.485GlnLeu: 3.485 ± 0.484
1.481GlnMet: 1.481 ± 0.348
2.178GlnAsn: 2.178 ± 0.461
0.784GlnPro: 0.784 ± 0.256
3.223GlnGln: 3.223 ± 0.898
1.829GlnArg: 1.829 ± 0.314
2.004GlnSer: 2.004 ± 0.364
3.398GlnThr: 3.398 ± 0.484
1.917GlnVal: 1.917 ± 0.37
0.697GlnTrp: 0.697 ± 0.237
2.091GlnTyr: 2.091 ± 0.311
0.0GlnXaa: 0.0 ± 0.0
Arg
2.352ArgAla: 2.352 ± 0.389
0.174ArgCys: 0.174 ± 0.137
2.526ArgAsp: 2.526 ± 0.475
2.613ArgGlu: 2.613 ± 0.445
2.178ArgPhe: 2.178 ± 0.45
2.265ArgGly: 2.265 ± 0.675
0.523ArgHis: 0.523 ± 0.218
3.223ArgIle: 3.223 ± 0.52
3.223ArgLys: 3.223 ± 0.486
3.659ArgLeu: 3.659 ± 0.771
0.697ArgMet: 0.697 ± 0.238
2.265ArgAsn: 2.265 ± 0.39
1.045ArgPro: 1.045 ± 0.239
2.439ArgGln: 2.439 ± 0.426
1.481ArgArg: 1.481 ± 0.377
1.568ArgSer: 1.568 ± 0.403
2.701ArgThr: 2.701 ± 0.617
2.178ArgVal: 2.178 ± 0.372
0.958ArgTrp: 0.958 ± 0.257
2.091ArgTyr: 2.091 ± 0.5
0.0ArgXaa: 0.0 ± 0.0
Ser
3.833SerAla: 3.833 ± 0.62
0.436SerCys: 0.436 ± 0.194
4.878SerAsp: 4.878 ± 0.527
3.92SerGlu: 3.92 ± 0.469
2.439SerPhe: 2.439 ± 0.53
4.617SerGly: 4.617 ± 0.651
0.697SerHis: 0.697 ± 0.271
4.878SerIle: 4.878 ± 0.628
5.314SerLys: 5.314 ± 0.774
4.53SerLeu: 4.53 ± 0.555
1.481SerMet: 1.481 ± 0.276
4.617SerAsn: 4.617 ± 0.982
1.742SerPro: 1.742 ± 0.294
2.788SerGln: 2.788 ± 0.495
3.049SerArg: 3.049 ± 0.669
2.962SerSer: 2.962 ± 0.477
4.269SerThr: 4.269 ± 0.848
4.617SerVal: 4.617 ± 0.731
0.61SerTrp: 0.61 ± 0.221
2.352SerTyr: 2.352 ± 0.476
0.0SerXaa: 0.0 ± 0.0
Thr
4.704ThrAla: 4.704 ± 0.654
0.436ThrCys: 0.436 ± 0.197
4.269ThrAsp: 4.269 ± 0.772
3.485ThrGlu: 3.485 ± 0.636
3.31ThrPhe: 3.31 ± 0.626
3.485ThrGly: 3.485 ± 0.515
1.394ThrHis: 1.394 ± 0.376
4.269ThrIle: 4.269 ± 0.859
6.098ThrLys: 6.098 ± 0.664
6.185ThrLeu: 6.185 ± 0.747
0.871ThrMet: 0.871 ± 0.248
4.356ThrAsn: 4.356 ± 0.847
2.004ThrPro: 2.004 ± 0.509
2.701ThrGln: 2.701 ± 0.796
1.568ThrArg: 1.568 ± 0.322
4.356ThrSer: 4.356 ± 0.617
3.659ThrThr: 3.659 ± 0.727
3.572ThrVal: 3.572 ± 0.447
1.133ThrTrp: 1.133 ± 0.344
3.746ThrTyr: 3.746 ± 0.741
0.0ThrXaa: 0.0 ± 0.0
Val
4.269ValAla: 4.269 ± 0.776
0.436ValCys: 0.436 ± 0.201
4.443ValAsp: 4.443 ± 0.472
3.659ValGlu: 3.659 ± 0.663
2.613ValPhe: 2.613 ± 0.655
3.659ValGly: 3.659 ± 0.493
0.697ValHis: 0.697 ± 0.197
2.962ValIle: 2.962 ± 0.425
4.269ValLys: 4.269 ± 0.58
2.788ValLeu: 2.788 ± 0.595
0.958ValMet: 0.958 ± 0.281
3.833ValAsn: 3.833 ± 0.698
2.265ValPro: 2.265 ± 0.428
2.265ValGln: 2.265 ± 0.621
2.265ValArg: 2.265 ± 0.637
4.966ValSer: 4.966 ± 0.678
4.966ValThr: 4.966 ± 0.844
3.398ValVal: 3.398 ± 0.558
1.22ValTrp: 1.22 ± 0.226
2.178ValTyr: 2.178 ± 0.45
0.0ValXaa: 0.0 ± 0.0
Trp
0.697TrpAla: 0.697 ± 0.225
0.0TrpCys: 0.0 ± 0.0
1.22TrpAsp: 1.22 ± 0.405
0.697TrpGlu: 0.697 ± 0.209
0.523TrpPhe: 0.523 ± 0.193
0.61TrpGly: 0.61 ± 0.243
0.436TrpHis: 0.436 ± 0.18
0.697TrpIle: 0.697 ± 0.204
0.871TrpLys: 0.871 ± 0.324
1.22TrpLeu: 1.22 ± 0.376
0.174TrpMet: 0.174 ± 0.117
0.871TrpAsn: 0.871 ± 0.312
0.174TrpPro: 0.174 ± 0.118
0.436TrpGln: 0.436 ± 0.177
0.871TrpArg: 0.871 ± 0.257
1.655TrpSer: 1.655 ± 0.525
1.22TrpThr: 1.22 ± 0.45
1.22TrpVal: 1.22 ± 0.267
0.348TrpTrp: 0.348 ± 0.222
0.436TrpTyr: 0.436 ± 0.182
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.613TyrAla: 2.613 ± 0.492
0.697TyrCys: 0.697 ± 0.314
2.701TyrAsp: 2.701 ± 0.511
2.613TyrGlu: 2.613 ± 0.625
2.178TyrPhe: 2.178 ± 0.42
2.352TyrGly: 2.352 ± 0.431
1.045TyrHis: 1.045 ± 0.297
2.788TyrIle: 2.788 ± 0.513
2.962TyrLys: 2.962 ± 0.496
3.136TyrLeu: 3.136 ± 0.451
1.133TyrMet: 1.133 ± 0.407
2.178TyrAsn: 2.178 ± 0.389
1.568TyrPro: 1.568 ± 0.37
2.178TyrGln: 2.178 ± 0.36
2.178TyrArg: 2.178 ± 0.362
2.962TyrSer: 2.962 ± 0.678
2.352TyrThr: 2.352 ± 0.949
2.526TyrVal: 2.526 ± 0.471
0.174TyrTrp: 0.174 ± 0.119
2.352TyrTyr: 2.352 ± 0.512
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 48 proteins (11480 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski