Amino acid dipepetide frequency for Streptococcus phage CHPC925

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.177AlaAla: 3.177 ± 1.136
0.289AlaCys: 0.289 ± 0.17
4.622AlaAsp: 4.622 ± 0.709
5.007AlaGlu: 5.007 ± 0.916
2.022AlaPhe: 2.022 ± 0.447
3.755AlaGly: 3.755 ± 0.672
0.77AlaHis: 0.77 ± 0.221
5.007AlaIle: 5.007 ± 0.76
6.258AlaLys: 6.258 ± 1.164
5.873AlaLeu: 5.873 ± 0.526
1.829AlaMet: 1.829 ± 0.51
4.622AlaAsn: 4.622 ± 0.719
1.348AlaPro: 1.348 ± 0.304
3.081AlaGln: 3.081 ± 0.431
3.177AlaArg: 3.177 ± 0.463
4.525AlaSer: 4.525 ± 0.791
3.948AlaThr: 3.948 ± 0.702
3.562AlaVal: 3.562 ± 0.63
0.77AlaTrp: 0.77 ± 0.219
2.696AlaTyr: 2.696 ± 0.499
0.0AlaXaa: 0.0 ± 0.0
Cys
0.481CysAla: 0.481 ± 0.254
0.0CysCys: 0.0 ± 0.0
0.481CysAsp: 0.481 ± 0.289
0.578CysGlu: 0.578 ± 0.307
0.193CysPhe: 0.193 ± 0.181
0.385CysGly: 0.385 ± 0.202
0.193CysHis: 0.193 ± 0.166
0.096CysIle: 0.096 ± 0.1
0.289CysLys: 0.289 ± 0.181
0.385CysLeu: 0.385 ± 0.267
0.096CysMet: 0.096 ± 0.09
0.385CysAsn: 0.385 ± 0.156
0.289CysPro: 0.289 ± 0.185
0.096CysGln: 0.096 ± 0.103
0.193CysArg: 0.193 ± 0.205
0.578CysSer: 0.578 ± 0.269
0.193CysThr: 0.193 ± 0.158
0.385CysVal: 0.385 ± 0.153
0.193CysTrp: 0.193 ± 0.147
0.193CysTyr: 0.193 ± 0.121
0.0CysXaa: 0.0 ± 0.0
Asp
3.274AspAla: 3.274 ± 0.54
0.481AspCys: 0.481 ± 0.187
3.562AspAsp: 3.562 ± 0.679
4.525AspGlu: 4.525 ± 0.656
3.851AspPhe: 3.851 ± 0.55
5.97AspGly: 5.97 ± 0.978
0.867AspHis: 0.867 ± 0.252
4.622AspIle: 4.622 ± 0.711
5.392AspLys: 5.392 ± 1.087
4.044AspLeu: 4.044 ± 0.792
2.311AspMet: 2.311 ± 0.465
5.199AspAsn: 5.199 ± 0.889
1.829AspPro: 1.829 ± 0.472
1.733AspGln: 1.733 ± 0.318
2.696AspArg: 2.696 ± 0.533
3.466AspSer: 3.466 ± 0.535
3.851AspThr: 3.851 ± 0.53
2.985AspVal: 2.985 ± 0.552
0.963AspTrp: 0.963 ± 0.393
2.503AspTyr: 2.503 ± 0.533
0.0AspXaa: 0.0 ± 0.0
Glu
4.044GluAla: 4.044 ± 0.671
0.289GluCys: 0.289 ± 0.146
3.948GluAsp: 3.948 ± 0.719
4.622GluGlu: 4.622 ± 1.118
2.215GluPhe: 2.215 ± 0.504
2.792GluGly: 2.792 ± 0.401
1.348GluHis: 1.348 ± 0.392
6.547GluIle: 6.547 ± 1.13
5.007GluLys: 5.007 ± 0.823
7.414GluLeu: 7.414 ± 1.068
2.503GluMet: 2.503 ± 0.537
3.755GluAsn: 3.755 ± 0.666
2.407GluPro: 2.407 ± 0.484
3.466GluGln: 3.466 ± 0.619
2.696GluArg: 2.696 ± 0.533
2.503GluSer: 2.503 ± 0.454
3.659GluThr: 3.659 ± 0.445
5.103GluVal: 5.103 ± 0.797
0.77GluTrp: 0.77 ± 0.333
3.177GluTyr: 3.177 ± 0.629
0.0GluXaa: 0.0 ± 0.0
Phe
3.177PheAla: 3.177 ± 0.568
0.481PheCys: 0.481 ± 0.187
3.851PheAsp: 3.851 ± 0.67
2.215PheGlu: 2.215 ± 0.529
1.637PhePhe: 1.637 ± 0.389
3.177PheGly: 3.177 ± 0.73
0.385PheHis: 0.385 ± 0.194
3.177PheIle: 3.177 ± 0.563
3.274PheLys: 3.274 ± 0.476
2.6PheLeu: 2.6 ± 0.527
0.963PheMet: 0.963 ± 0.258
3.37PheAsn: 3.37 ± 0.637
0.674PhePro: 0.674 ± 0.225
1.252PheGln: 1.252 ± 0.299
1.541PheArg: 1.541 ± 0.327
2.503PheSer: 2.503 ± 0.585
2.6PheThr: 2.6 ± 0.588
3.081PheVal: 3.081 ± 0.711
0.674PheTrp: 0.674 ± 0.24
1.829PheTyr: 1.829 ± 0.407
0.0PheXaa: 0.0 ± 0.0
Gly
3.562GlyAla: 3.562 ± 0.635
0.289GlyCys: 0.289 ± 0.179
3.659GlyAsp: 3.659 ± 0.74
3.562GlyGlu: 3.562 ± 0.701
3.562GlyPhe: 3.562 ± 0.453
4.429GlyGly: 4.429 ± 0.858
1.059GlyHis: 1.059 ± 0.301
4.622GlyIle: 4.622 ± 1.015
6.451GlyLys: 6.451 ± 0.916
5.777GlyLeu: 5.777 ± 0.736
1.444GlyMet: 1.444 ± 0.311
4.429GlyAsn: 4.429 ± 0.909
0.963GlyPro: 0.963 ± 0.363
2.889GlyGln: 2.889 ± 0.535
2.311GlyArg: 2.311 ± 0.519
4.429GlySer: 4.429 ± 0.759
3.755GlyThr: 3.755 ± 0.635
3.948GlyVal: 3.948 ± 0.814
1.252GlyTrp: 1.252 ± 0.389
3.37GlyTyr: 3.37 ± 0.581
0.0GlyXaa: 0.0 ± 0.0
His
0.578HisAla: 0.578 ± 0.266
0.0HisCys: 0.0 ± 0.0
1.059HisAsp: 1.059 ± 0.312
0.193HisGlu: 0.193 ± 0.153
0.867HisPhe: 0.867 ± 0.264
0.674HisGly: 0.674 ± 0.225
0.481HisHis: 0.481 ± 0.222
0.963HisIle: 0.963 ± 0.324
1.541HisLys: 1.541 ± 0.402
0.867HisLeu: 0.867 ± 0.275
0.674HisMet: 0.674 ± 0.267
1.155HisAsn: 1.155 ± 0.278
0.481HisPro: 0.481 ± 0.194
0.867HisGln: 0.867 ± 0.32
0.867HisArg: 0.867 ± 0.243
1.252HisSer: 1.252 ± 0.261
1.059HisThr: 1.059 ± 0.3
1.155HisVal: 1.155 ± 0.248
0.096HisTrp: 0.096 ± 0.082
0.578HisTyr: 0.578 ± 0.267
0.0HisXaa: 0.0 ± 0.0
Ile
5.488IleAla: 5.488 ± 0.917
0.385IleCys: 0.385 ± 0.191
5.103IleAsp: 5.103 ± 0.621
5.488IleGlu: 5.488 ± 1.071
1.829IlePhe: 1.829 ± 0.456
3.948IleGly: 3.948 ± 0.527
1.059IleHis: 1.059 ± 0.309
2.118IleIle: 2.118 ± 0.402
6.547IleLys: 6.547 ± 0.886
4.044IleLeu: 4.044 ± 0.771
1.829IleMet: 1.829 ± 0.489
3.851IleAsn: 3.851 ± 0.498
3.177IlePro: 3.177 ± 0.604
3.177IleGln: 3.177 ± 0.59
2.118IleArg: 2.118 ± 0.339
5.007IleSer: 5.007 ± 0.546
4.429IleThr: 4.429 ± 0.665
3.466IleVal: 3.466 ± 0.582
1.059IleTrp: 1.059 ± 0.341
2.118IleTyr: 2.118 ± 0.416
0.0IleXaa: 0.0 ± 0.0
Lys
5.97LysAla: 5.97 ± 0.648
0.385LysCys: 0.385 ± 0.196
5.392LysAsp: 5.392 ± 0.779
7.029LysGlu: 7.029 ± 0.821
3.659LysPhe: 3.659 ± 0.811
5.392LysGly: 5.392 ± 0.701
1.348LysHis: 1.348 ± 0.472
7.703LysIle: 7.703 ± 0.985
7.029LysLys: 7.029 ± 0.998
6.258LysLeu: 6.258 ± 0.799
2.696LysMet: 2.696 ± 0.575
4.236LysAsn: 4.236 ± 0.83
2.792LysPro: 2.792 ± 0.5
4.333LysGln: 4.333 ± 0.606
4.14LysArg: 4.14 ± 0.747
4.044LysSer: 4.044 ± 0.781
5.199LysThr: 5.199 ± 0.956
3.948LysVal: 3.948 ± 0.715
1.444LysTrp: 1.444 ± 0.313
3.37LysTyr: 3.37 ± 0.579
0.0LysXaa: 0.0 ± 0.0
Leu
6.258LeuAla: 6.258 ± 0.843
0.481LeuCys: 0.481 ± 0.231
6.066LeuAsp: 6.066 ± 0.913
6.451LeuGlu: 6.451 ± 0.905
3.274LeuPhe: 3.274 ± 0.485
5.103LeuGly: 5.103 ± 0.87
1.059LeuHis: 1.059 ± 0.407
3.948LeuIle: 3.948 ± 0.602
8.377LeuLys: 8.377 ± 0.898
3.851LeuLeu: 3.851 ± 0.663
2.696LeuMet: 2.696 ± 0.495
4.14LeuAsn: 4.14 ± 0.734
2.118LeuPro: 2.118 ± 0.402
3.177LeuGln: 3.177 ± 0.557
3.274LeuArg: 3.274 ± 0.797
4.333LeuSer: 4.333 ± 0.791
5.777LeuThr: 5.777 ± 0.961
3.37LeuVal: 3.37 ± 0.533
0.385LeuTrp: 0.385 ± 0.155
2.311LeuTyr: 2.311 ± 0.596
0.0LeuXaa: 0.0 ± 0.0
Met
2.118MetAla: 2.118 ± 0.425
0.096MetCys: 0.096 ± 0.104
0.77MetAsp: 0.77 ± 0.249
1.444MetGlu: 1.444 ± 0.436
1.059MetPhe: 1.059 ± 0.3
0.963MetGly: 0.963 ± 0.335
0.385MetHis: 0.385 ± 0.206
1.926MetIle: 1.926 ± 0.348
3.081MetLys: 3.081 ± 0.573
2.215MetLeu: 2.215 ± 0.329
0.674MetMet: 0.674 ± 0.248
1.348MetAsn: 1.348 ± 0.35
0.963MetPro: 0.963 ± 0.276
0.674MetGln: 0.674 ± 0.234
0.963MetArg: 0.963 ± 0.239
2.118MetSer: 2.118 ± 0.456
2.215MetThr: 2.215 ± 0.373
2.215MetVal: 2.215 ± 0.53
0.096MetTrp: 0.096 ± 0.082
0.963MetTyr: 0.963 ± 0.259
0.0MetXaa: 0.0 ± 0.0
Asn
5.007AsnAla: 5.007 ± 0.988
0.289AsnCys: 0.289 ± 0.172
3.659AsnAsp: 3.659 ± 0.634
3.755AsnGlu: 3.755 ± 0.711
3.177AsnPhe: 3.177 ± 0.475
6.355AsnGly: 6.355 ± 1.05
1.155AsnHis: 1.155 ± 0.35
3.177AsnIle: 3.177 ± 0.55
4.044AsnLys: 4.044 ± 0.551
4.91AsnLeu: 4.91 ± 0.753
1.252AsnMet: 1.252 ± 0.322
4.91AsnAsn: 4.91 ± 0.905
3.177AsnPro: 3.177 ± 0.726
3.37AsnGln: 3.37 ± 0.656
2.696AsnArg: 2.696 ± 0.558
4.91AsnSer: 4.91 ± 0.63
2.985AsnThr: 2.985 ± 0.621
3.466AsnVal: 3.466 ± 0.483
0.77AsnTrp: 0.77 ± 0.235
2.022AsnTyr: 2.022 ± 0.394
0.0AsnXaa: 0.0 ± 0.0
Pro
1.637ProAla: 1.637 ± 0.3
0.0ProCys: 0.0 ± 0.0
1.059ProAsp: 1.059 ± 0.351
2.118ProGlu: 2.118 ± 0.514
1.059ProPhe: 1.059 ± 0.32
1.252ProGly: 1.252 ± 0.453
0.385ProHis: 0.385 ± 0.18
1.541ProIle: 1.541 ± 0.276
3.755ProLys: 3.755 ± 0.691
2.407ProLeu: 2.407 ± 0.471
0.193ProMet: 0.193 ± 0.128
2.311ProAsn: 2.311 ± 0.478
0.289ProPro: 0.289 ± 0.224
1.637ProGln: 1.637 ± 0.296
0.963ProArg: 0.963 ± 0.446
2.118ProSer: 2.118 ± 0.507
2.407ProThr: 2.407 ± 0.394
1.348ProVal: 1.348 ± 0.357
0.77ProTrp: 0.77 ± 0.242
0.963ProTyr: 0.963 ± 0.364
0.0ProXaa: 0.0 ± 0.0
Gln
4.525GlnAla: 4.525 ± 0.601
0.481GlnCys: 0.481 ± 0.256
2.889GlnAsp: 2.889 ± 0.492
3.081GlnGlu: 3.081 ± 0.66
1.444GlnPhe: 1.444 ± 0.394
3.081GlnGly: 3.081 ± 0.582
0.77GlnHis: 0.77 ± 0.253
2.889GlnIle: 2.889 ± 0.625
2.985GlnLys: 2.985 ± 0.579
3.659GlnLeu: 3.659 ± 0.554
1.733GlnMet: 1.733 ± 0.321
2.407GlnAsn: 2.407 ± 0.377
0.481GlnPro: 0.481 ± 0.188
3.466GlnGln: 3.466 ± 0.588
2.215GlnArg: 2.215 ± 0.428
2.696GlnSer: 2.696 ± 0.543
2.985GlnThr: 2.985 ± 0.511
2.118GlnVal: 2.118 ± 0.515
0.77GlnTrp: 0.77 ± 0.275
2.022GlnTyr: 2.022 ± 0.389
0.0GlnXaa: 0.0 ± 0.0
Arg
2.118ArgAla: 2.118 ± 0.419
0.096ArgCys: 0.096 ± 0.103
2.311ArgAsp: 2.311 ± 0.522
3.562ArgGlu: 3.562 ± 0.651
1.829ArgPhe: 1.829 ± 0.407
2.118ArgGly: 2.118 ± 0.637
0.674ArgHis: 0.674 ± 0.22
2.792ArgIle: 2.792 ± 0.515
3.755ArgLys: 3.755 ± 0.694
3.659ArgLeu: 3.659 ± 0.68
0.481ArgMet: 0.481 ± 0.226
2.985ArgAsn: 2.985 ± 0.405
1.252ArgPro: 1.252 ± 0.366
2.503ArgGln: 2.503 ± 0.432
1.541ArgArg: 1.541 ± 0.373
1.541ArgSer: 1.541 ± 0.409
2.215ArgThr: 2.215 ± 0.678
2.792ArgVal: 2.792 ± 0.52
0.963ArgTrp: 0.963 ± 0.262
2.407ArgTyr: 2.407 ± 0.638
0.0ArgXaa: 0.0 ± 0.0
Ser
3.851SerAla: 3.851 ± 0.555
0.578SerCys: 0.578 ± 0.265
4.429SerAsp: 4.429 ± 0.555
3.466SerGlu: 3.466 ± 0.479
2.407SerPhe: 2.407 ± 0.407
4.718SerGly: 4.718 ± 0.646
0.578SerHis: 0.578 ± 0.191
3.948SerIle: 3.948 ± 0.658
4.814SerLys: 4.814 ± 0.658
3.948SerLeu: 3.948 ± 0.75
2.022SerMet: 2.022 ± 0.37
5.103SerAsn: 5.103 ± 0.577
1.829SerPro: 1.829 ± 0.368
2.696SerGln: 2.696 ± 0.59
3.081SerArg: 3.081 ± 0.59
2.889SerSer: 2.889 ± 0.482
4.236SerThr: 4.236 ± 0.747
4.14SerVal: 4.14 ± 0.532
1.059SerTrp: 1.059 ± 0.215
1.637SerTyr: 1.637 ± 0.384
0.0SerXaa: 0.0 ± 0.0
Thr
3.851ThrAla: 3.851 ± 0.578
0.289ThrCys: 0.289 ± 0.164
3.755ThrAsp: 3.755 ± 0.57
2.985ThrGlu: 2.985 ± 0.534
3.081ThrPhe: 3.081 ± 0.555
4.044ThrGly: 4.044 ± 0.561
1.155ThrHis: 1.155 ± 0.307
4.429ThrIle: 4.429 ± 0.687
4.91ThrLys: 4.91 ± 0.727
5.488ThrLeu: 5.488 ± 0.736
0.77ThrMet: 0.77 ± 0.209
4.622ThrAsn: 4.622 ± 0.689
1.155ThrPro: 1.155 ± 0.409
3.081ThrGln: 3.081 ± 0.619
2.407ThrArg: 2.407 ± 0.463
3.466ThrSer: 3.466 ± 0.698
3.562ThrThr: 3.562 ± 0.664
4.333ThrVal: 4.333 ± 0.617
1.059ThrTrp: 1.059 ± 0.266
2.503ThrTyr: 2.503 ± 0.529
0.0ThrXaa: 0.0 ± 0.0
Val
3.948ValAla: 3.948 ± 0.711
0.385ValCys: 0.385 ± 0.191
3.948ValAsp: 3.948 ± 0.471
4.236ValGlu: 4.236 ± 0.734
2.792ValPhe: 2.792 ± 0.504
5.103ValGly: 5.103 ± 0.705
0.578ValHis: 0.578 ± 0.19
3.659ValIle: 3.659 ± 0.572
5.007ValLys: 5.007 ± 0.637
4.333ValLeu: 4.333 ± 0.66
0.867ValMet: 0.867 ± 0.233
3.562ValAsn: 3.562 ± 0.709
1.733ValPro: 1.733 ± 0.466
2.215ValGln: 2.215 ± 0.541
1.829ValArg: 1.829 ± 0.383
5.296ValSer: 5.296 ± 0.709
3.562ValThr: 3.562 ± 0.654
4.044ValVal: 4.044 ± 0.699
0.963ValTrp: 0.963 ± 0.221
1.348ValTyr: 1.348 ± 0.427
0.0ValXaa: 0.0 ± 0.0
Trp
0.77TrpAla: 0.77 ± 0.256
0.0TrpCys: 0.0 ± 0.0
0.963TrpAsp: 0.963 ± 0.535
1.059TrpGlu: 1.059 ± 0.262
0.481TrpPhe: 0.481 ± 0.203
0.481TrpGly: 0.481 ± 0.193
0.385TrpHis: 0.385 ± 0.211
0.867TrpIle: 0.867 ± 0.258
0.674TrpLys: 0.674 ± 0.27
1.348TrpLeu: 1.348 ± 0.365
0.289TrpMet: 0.289 ± 0.196
0.963TrpAsn: 0.963 ± 0.267
0.096TrpPro: 0.096 ± 0.105
0.77TrpGln: 0.77 ± 0.22
0.963TrpArg: 0.963 ± 0.251
1.637TrpSer: 1.637 ± 0.587
0.674TrpThr: 0.674 ± 0.256
1.444TrpVal: 1.444 ± 0.275
0.289TrpTrp: 0.289 ± 0.191
0.385TrpTyr: 0.385 ± 0.186
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.503TyrAla: 2.503 ± 0.432
0.385TyrCys: 0.385 ± 0.194
2.6TyrAsp: 2.6 ± 0.457
2.889TyrGlu: 2.889 ± 0.594
2.022TyrPhe: 2.022 ± 0.454
2.022TyrGly: 2.022 ± 0.533
0.77TyrHis: 0.77 ± 0.277
2.215TyrIle: 2.215 ± 0.387
3.177TyrLys: 3.177 ± 0.565
3.37TyrLeu: 3.37 ± 0.64
0.867TyrMet: 0.867 ± 0.251
1.733TyrAsn: 1.733 ± 0.394
1.155TyrPro: 1.155 ± 0.336
2.215TyrGln: 2.215 ± 0.372
2.022TyrArg: 2.022 ± 0.379
2.118TyrSer: 2.118 ± 0.554
1.637TyrThr: 1.637 ± 0.437
2.503TyrVal: 2.503 ± 0.451
0.193TyrTrp: 0.193 ± 0.152
2.311TyrTyr: 2.311 ± 0.542
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 45 proteins (10387 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski