Gryllotalpicola protaetiae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Gryllotalpicola

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3481 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A387BJ94|A0A387BJ94_9MICO PadR family transcriptional regulator OS=Gryllotalpicola protaetiae OX=2419771 GN=D7I44_04085 PE=4 SV=1
MM1 pKa = 7.26SVSFTPYY8 pKa = 10.46LSFRR12 pKa = 11.84GQARR16 pKa = 11.84EE17 pKa = 3.79AMNFYY22 pKa = 10.53QSVFGGEE29 pKa = 4.05LTVTTFADD37 pKa = 3.45GGMTDD42 pKa = 4.76GVDD45 pKa = 3.84PNQIMHH51 pKa = 6.24SQLDD55 pKa = 4.04GAVSLMGSDD64 pKa = 4.38TPDD67 pKa = 2.94SMPYY71 pKa = 9.51TDD73 pKa = 4.51GSRR76 pKa = 11.84ITLAFSGDD84 pKa = 3.61SAGLDD89 pKa = 3.5SCRR92 pKa = 11.84DD93 pKa = 3.7YY94 pKa = 11.15YY95 pKa = 11.7AKK97 pKa = 10.85LGDD100 pKa = 3.97GGSNVLPLDD109 pKa = 4.08LAPWGDD115 pKa = 4.04YY116 pKa = 10.55YY117 pKa = 11.67GQLTDD122 pKa = 4.08KK123 pKa = 11.47YY124 pKa = 9.8GITWMFDD131 pKa = 2.89FSAPEE136 pKa = 4.06VPDD139 pKa = 4.28DD140 pKa = 4.25ASSLRR145 pKa = 11.84AHH147 pKa = 6.3SQDD150 pKa = 3.34SLL152 pKa = 3.76

Molecular weight: 16.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A387BSK5|A0A387BSK5_9MICO Alpha/beta fold hydrolase OS=Gryllotalpicola protaetiae OX=2419771 GN=D7I44_10460 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3481

0

3481

1118351

28

2296

321.3

34.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.931 ± 0.055

0.484 ± 0.01

5.855 ± 0.047

5.381 ± 0.046

3.24 ± 0.029

9.017 ± 0.044

2.039 ± 0.018

4.438 ± 0.033

2.191 ± 0.032

10.229 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.587 ± 0.017

2.094 ± 0.027

5.356 ± 0.029

2.994 ± 0.026

7.003 ± 0.055

5.755 ± 0.04

6.003 ± 0.044

8.792 ± 0.034

1.558 ± 0.019

2.053 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski