Salmonella phage SE4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Loughboroughvirus; unclassified Loughboroughvirus

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9N4T5|A0A5B9N4T5_9CAUD Uncharacterized protein OS=Salmonella phage SE4 OX=2575328 PE=4 SV=1
MM1 pKa = 7.83DD2 pKa = 3.39WFMRR6 pKa = 11.84IFQEE10 pKa = 4.75EE11 pKa = 4.07PAEE14 pKa = 4.23VAKK17 pKa = 10.67KK18 pKa = 10.28LYY20 pKa = 10.38KK21 pKa = 10.5SGINCYY27 pKa = 10.35DD28 pKa = 2.78ITALCFEE35 pKa = 4.88IRR37 pKa = 11.84GEE39 pKa = 3.99DD40 pKa = 3.83DD41 pKa = 3.21AWTQEE46 pKa = 4.08LCDD49 pKa = 3.99EE50 pKa = 5.12LEE52 pKa = 4.28TIYY55 pKa = 11.14CEE57 pKa = 4.43EE58 pKa = 4.04EE59 pKa = 4.28CKK61 pKa = 10.83

Molecular weight: 7.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9N8J0|A0A5B9N8J0_9CAUD Uncharacterized protein OS=Salmonella phage SE4 OX=2575328 PE=4 SV=1
MM1 pKa = 7.35FRR3 pKa = 11.84IRR5 pKa = 11.84LEE7 pKa = 3.92RR8 pKa = 11.84RR9 pKa = 11.84DD10 pKa = 3.51KK11 pKa = 11.14QKK13 pKa = 11.29FEE15 pKa = 3.54FTYY18 pKa = 10.71RR19 pKa = 11.84AFKK22 pKa = 10.65SFNGQGVTVGVHH34 pKa = 5.66PDD36 pKa = 2.78KK37 pKa = 11.03NRR39 pKa = 11.84RR40 pKa = 11.84SDD42 pKa = 3.49PKK44 pKa = 10.94DD45 pKa = 3.69GIGNAEE51 pKa = 4.07LAMVHH56 pKa = 6.8EE57 pKa = 4.9YY58 pKa = 11.27GLNNVPEE65 pKa = 4.24RR66 pKa = 11.84SFLRR70 pKa = 11.84SAVGGRR76 pKa = 11.84GKK78 pKa = 10.48GRR80 pKa = 11.84KK81 pKa = 9.1AINRR85 pKa = 11.84AFKK88 pKa = 10.39EE89 pKa = 4.11GVPAVLRR96 pKa = 11.84GDD98 pKa = 3.61MTAEE102 pKa = 4.25DD103 pKa = 4.13LNDD106 pKa = 4.33RR107 pKa = 11.84IGRR110 pKa = 11.84MLVDD114 pKa = 3.95AVHH117 pKa = 5.98EE118 pKa = 4.28RR119 pKa = 11.84MRR121 pKa = 11.84EE122 pKa = 4.05NIPPPLKK129 pKa = 10.64PEE131 pKa = 4.04TEE133 pKa = 4.12RR134 pKa = 11.84KK135 pKa = 7.95KK136 pKa = 9.33TGPGTLRR143 pKa = 11.84EE144 pKa = 4.15SMEE147 pKa = 4.61LYY149 pKa = 10.72DD150 pKa = 4.44SIGYY154 pKa = 9.35KK155 pKa = 9.94VGRR158 pKa = 11.84NWRR161 pKa = 11.84LL162 pKa = 2.91

Molecular weight: 18.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

16383

40

986

215.6

24.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.46 ± 0.295

1.105 ± 0.103

6.659 ± 0.216

6.141 ± 0.319

3.589 ± 0.16

7.062 ± 0.281

1.721 ± 0.129

5.835 ± 0.166

6.153 ± 0.334

7.959 ± 0.266

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.863 ± 0.152

4.932 ± 0.205

4.419 ± 0.211

3.937 ± 0.167

4.59 ± 0.249

5.579 ± 0.251

6.641 ± 0.476

7.251 ± 0.32

1.636 ± 0.156

3.467 ± 0.235
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski