Amino acid dipepetide frequency for Salmonella phage SE4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.997AlaAla: 7.997 ± 0.981
0.549AlaCys: 0.549 ± 0.194
5.921AlaAsp: 5.921 ± 0.587
5.921AlaGlu: 5.921 ± 0.957
2.259AlaPhe: 2.259 ± 0.36
6.104AlaGly: 6.104 ± 0.678
1.343AlaHis: 1.343 ± 0.309
5.25AlaIle: 5.25 ± 0.493
5.921AlaLys: 5.921 ± 0.73
5.86AlaLeu: 5.86 ± 0.674
2.259AlaMet: 2.259 ± 0.362
4.639AlaAsn: 4.639 ± 0.573
2.686AlaPro: 2.686 ± 0.441
2.808AlaGln: 2.808 ± 0.456
3.785AlaArg: 3.785 ± 0.549
5.005AlaSer: 5.005 ± 0.531
6.287AlaThr: 6.287 ± 1.185
6.165AlaVal: 6.165 ± 0.584
1.404AlaTrp: 1.404 ± 0.337
2.442AlaTyr: 2.442 ± 0.326
0.0AlaXaa: 0.0 ± 0.0
Cys
0.61CysAla: 0.61 ± 0.205
0.183CysCys: 0.183 ± 0.096
0.61CysAsp: 0.61 ± 0.145
0.916CysGlu: 0.916 ± 0.208
0.61CysPhe: 0.61 ± 0.216
0.671CysGly: 0.671 ± 0.203
0.183CysHis: 0.183 ± 0.088
0.366CysIle: 0.366 ± 0.125
0.794CysLys: 0.794 ± 0.185
0.977CysLeu: 0.977 ± 0.295
0.305CysMet: 0.305 ± 0.166
0.549CysAsn: 0.549 ± 0.176
0.305CysPro: 0.305 ± 0.135
0.427CysGln: 0.427 ± 0.148
0.733CysArg: 0.733 ± 0.201
0.366CysSer: 0.366 ± 0.179
0.61CysThr: 0.61 ± 0.195
1.099CysVal: 1.099 ± 0.299
0.061CysTrp: 0.061 ± 0.069
0.671CysTyr: 0.671 ± 0.185
0.0CysXaa: 0.0 ± 0.0
Asp
5.616AspAla: 5.616 ± 0.593
0.671AspCys: 0.671 ± 0.182
4.395AspAsp: 4.395 ± 0.483
5.005AspGlu: 5.005 ± 0.545
2.93AspPhe: 2.93 ± 0.386
5.25AspGly: 5.25 ± 0.56
0.794AspHis: 0.794 ± 0.263
5.494AspIle: 5.494 ± 0.577
4.151AspLys: 4.151 ± 0.556
5.738AspLeu: 5.738 ± 0.545
1.465AspMet: 1.465 ± 0.328
3.907AspAsn: 3.907 ± 0.684
2.93AspPro: 2.93 ± 0.389
2.198AspGln: 2.198 ± 0.348
2.075AspArg: 2.075 ± 0.329
3.235AspSer: 3.235 ± 0.451
3.235AspThr: 3.235 ± 0.618
4.212AspVal: 4.212 ± 0.399
1.526AspTrp: 1.526 ± 0.391
1.709AspTyr: 1.709 ± 0.286
0.0AspXaa: 0.0 ± 0.0
Glu
5.86GluAla: 5.86 ± 0.825
0.733GluCys: 0.733 ± 0.204
2.991GluAsp: 2.991 ± 0.34
4.7GluGlu: 4.7 ± 0.677
2.198GluPhe: 2.198 ± 0.405
4.273GluGly: 4.273 ± 0.492
1.099GluHis: 1.099 ± 0.269
2.747GluIle: 2.747 ± 0.385
3.113GluLys: 3.113 ± 0.608
7.447GluLeu: 7.447 ± 0.805
2.442GluMet: 2.442 ± 0.438
2.075GluAsn: 2.075 ± 0.316
2.136GluPro: 2.136 ± 0.331
2.808GluGln: 2.808 ± 0.422
3.235GluArg: 3.235 ± 0.441
3.724GluSer: 3.724 ± 0.438
2.381GluThr: 2.381 ± 0.293
4.151GluVal: 4.151 ± 0.449
1.038GluTrp: 1.038 ± 0.249
2.259GluTyr: 2.259 ± 0.413
0.0GluXaa: 0.0 ± 0.0
Phe
1.892PheAla: 1.892 ± 0.311
0.549PheCys: 0.549 ± 0.186
2.503PheAsp: 2.503 ± 0.393
2.014PheGlu: 2.014 ± 0.359
1.099PhePhe: 1.099 ± 0.217
2.747PheGly: 2.747 ± 0.312
0.61PheHis: 0.61 ± 0.173
2.259PheIle: 2.259 ± 0.384
2.503PheLys: 2.503 ± 0.386
1.953PheLeu: 1.953 ± 0.311
0.794PheMet: 0.794 ± 0.2
2.625PheAsn: 2.625 ± 0.361
1.16PhePro: 1.16 ± 0.197
1.343PheGln: 1.343 ± 0.274
1.953PheArg: 1.953 ± 0.293
2.747PheSer: 2.747 ± 0.457
3.174PheThr: 3.174 ± 0.514
2.503PheVal: 2.503 ± 0.377
0.427PheTrp: 0.427 ± 0.185
1.038PheTyr: 1.038 ± 0.255
0.0PheXaa: 0.0 ± 0.0
Gly
5.311GlyAla: 5.311 ± 0.63
0.916GlyCys: 0.916 ± 0.248
3.968GlyAsp: 3.968 ± 0.507
2.808GlyGlu: 2.808 ± 0.4
2.747GlyPhe: 2.747 ± 0.468
6.043GlyGly: 6.043 ± 0.758
1.526GlyHis: 1.526 ± 0.296
5.067GlyIle: 5.067 ± 0.511
5.189GlyLys: 5.189 ± 0.545
5.372GlyLeu: 5.372 ± 0.459
1.648GlyMet: 1.648 ± 0.396
3.235GlyAsn: 3.235 ± 0.333
2.198GlyPro: 2.198 ± 0.342
3.113GlyGln: 3.113 ± 0.378
3.174GlyArg: 3.174 ± 0.533
4.029GlySer: 4.029 ± 0.507
4.761GlyThr: 4.761 ± 0.658
5.189GlyVal: 5.189 ± 0.462
1.587GlyTrp: 1.587 ± 0.312
2.747GlyTyr: 2.747 ± 0.42
0.0GlyXaa: 0.0 ± 0.0
His
1.16HisAla: 1.16 ± 0.227
0.305HisCys: 0.305 ± 0.134
1.038HisAsp: 1.038 ± 0.275
1.221HisGlu: 1.221 ± 0.334
0.671HisPhe: 0.671 ± 0.178
0.855HisGly: 0.855 ± 0.24
0.549HisHis: 0.549 ± 0.173
1.343HisIle: 1.343 ± 0.266
0.794HisLys: 0.794 ± 0.243
1.587HisLeu: 1.587 ± 0.33
0.855HisMet: 0.855 ± 0.199
0.855HisAsn: 0.855 ± 0.229
0.916HisPro: 0.916 ± 0.19
0.671HisGln: 0.671 ± 0.217
0.549HisArg: 0.549 ± 0.197
0.855HisSer: 0.855 ± 0.228
0.855HisThr: 0.855 ± 0.208
0.855HisVal: 0.855 ± 0.204
0.427HisTrp: 0.427 ± 0.128
0.855HisTyr: 0.855 ± 0.19
0.0HisXaa: 0.0 ± 0.0
Ile
5.433IleAla: 5.433 ± 0.64
0.488IleCys: 0.488 ± 0.191
4.273IleAsp: 4.273 ± 0.49
3.418IleGlu: 3.418 ± 0.435
2.014IlePhe: 2.014 ± 0.328
3.724IleGly: 3.724 ± 0.54
0.977IleHis: 0.977 ± 0.217
2.747IleIle: 2.747 ± 0.457
3.785IleLys: 3.785 ± 0.388
4.273IleLeu: 4.273 ± 0.516
1.282IleMet: 1.282 ± 0.281
4.151IleAsn: 4.151 ± 0.463
2.686IlePro: 2.686 ± 0.411
2.32IleGln: 2.32 ± 0.311
2.503IleArg: 2.503 ± 0.314
2.564IleSer: 2.564 ± 0.451
4.822IleThr: 4.822 ± 0.591
3.724IleVal: 3.724 ± 0.483
0.733IleTrp: 0.733 ± 0.239
2.442IleTyr: 2.442 ± 0.449
0.0IleXaa: 0.0 ± 0.0
Lys
6.532LysAla: 6.532 ± 0.937
0.305LysCys: 0.305 ± 0.125
3.907LysAsp: 3.907 ± 0.584
4.517LysGlu: 4.517 ± 0.597
2.32LysPhe: 2.32 ± 0.396
3.418LysGly: 3.418 ± 0.379
1.16LysHis: 1.16 ± 0.274
2.442LysIle: 2.442 ± 0.34
3.479LysLys: 3.479 ± 0.601
5.555LysLeu: 5.555 ± 0.714
2.442LysMet: 2.442 ± 0.35
2.625LysAsn: 2.625 ± 0.434
3.663LysPro: 3.663 ± 0.461
2.564LysGln: 2.564 ± 0.425
2.686LysArg: 2.686 ± 0.485
3.357LysSer: 3.357 ± 0.447
3.968LysThr: 3.968 ± 0.45
4.151LysVal: 4.151 ± 0.457
0.549LysTrp: 0.549 ± 0.21
1.892LysTyr: 1.892 ± 0.358
0.0LysXaa: 0.0 ± 0.0
Leu
6.898LeuAla: 6.898 ± 0.629
1.099LeuCys: 1.099 ± 0.23
5.372LeuAsp: 5.372 ± 0.554
4.944LeuGlu: 4.944 ± 0.461
2.808LeuPhe: 2.808 ± 0.359
4.639LeuGly: 4.639 ± 0.52
1.221LeuHis: 1.221 ± 0.282
3.968LeuIle: 3.968 ± 0.446
5.433LeuLys: 5.433 ± 0.6
5.311LeuLeu: 5.311 ± 0.571
2.503LeuMet: 2.503 ± 0.432
3.296LeuAsn: 3.296 ± 0.5
4.944LeuPro: 4.944 ± 0.595
3.113LeuGln: 3.113 ± 0.462
4.578LeuArg: 4.578 ± 0.494
4.151LeuSer: 4.151 ± 0.438
6.593LeuThr: 6.593 ± 0.641
5.005LeuVal: 5.005 ± 0.587
1.282LeuTrp: 1.282 ± 0.324
2.442LeuTyr: 2.442 ± 0.351
0.0LeuXaa: 0.0 ± 0.0
Met
2.381MetAla: 2.381 ± 0.304
0.427MetCys: 0.427 ± 0.156
2.014MetAsp: 2.014 ± 0.324
1.892MetGlu: 1.892 ± 0.389
1.038MetPhe: 1.038 ± 0.237
1.526MetGly: 1.526 ± 0.238
0.427MetHis: 0.427 ± 0.149
1.404MetIle: 1.404 ± 0.285
1.892MetLys: 1.892 ± 0.371
2.503MetLeu: 2.503 ± 0.323
0.671MetMet: 0.671 ± 0.14
1.221MetAsn: 1.221 ± 0.257
1.526MetPro: 1.526 ± 0.281
1.038MetGln: 1.038 ± 0.23
2.014MetArg: 2.014 ± 0.355
1.892MetSer: 1.892 ± 0.275
1.892MetThr: 1.892 ± 0.369
1.648MetVal: 1.648 ± 0.314
0.061MetTrp: 0.061 ± 0.068
1.16MetTyr: 1.16 ± 0.257
0.0MetXaa: 0.0 ± 0.0
Asn
4.151AsnAla: 4.151 ± 0.464
0.855AsnCys: 0.855 ± 0.202
2.869AsnAsp: 2.869 ± 0.42
2.747AsnGlu: 2.747 ± 0.353
2.136AsnPhe: 2.136 ± 0.364
4.395AsnGly: 4.395 ± 0.486
0.427AsnHis: 0.427 ± 0.159
3.174AsnIle: 3.174 ± 0.45
2.686AsnLys: 2.686 ± 0.39
3.663AsnLeu: 3.663 ± 0.367
1.221AsnMet: 1.221 ± 0.259
2.32AsnAsn: 2.32 ± 0.399
2.686AsnPro: 2.686 ± 0.456
2.014AsnGln: 2.014 ± 0.417
2.075AsnArg: 2.075 ± 0.407
2.442AsnSer: 2.442 ± 0.339
3.357AsnThr: 3.357 ± 0.404
3.418AsnVal: 3.418 ± 0.454
1.526AsnTrp: 1.526 ± 0.292
1.16AsnTyr: 1.16 ± 0.285
0.0AsnXaa: 0.0 ± 0.0
Pro
3.663ProAla: 3.663 ± 0.509
0.427ProCys: 0.427 ± 0.148
3.846ProAsp: 3.846 ± 0.361
2.869ProGlu: 2.869 ± 0.407
1.648ProPhe: 1.648 ± 0.317
2.869ProGly: 2.869 ± 0.477
0.733ProHis: 0.733 ± 0.207
2.136ProIle: 2.136 ± 0.431
2.381ProLys: 2.381 ± 0.377
3.479ProLeu: 3.479 ± 0.414
1.404ProMet: 1.404 ± 0.313
1.953ProAsn: 1.953 ± 0.399
2.198ProPro: 2.198 ± 0.387
1.709ProGln: 1.709 ± 0.309
1.892ProArg: 1.892 ± 0.371
2.381ProSer: 2.381 ± 0.407
3.174ProThr: 3.174 ± 0.46
3.479ProVal: 3.479 ± 0.451
0.549ProTrp: 0.549 ± 0.15
1.404ProTyr: 1.404 ± 0.313
0.0ProXaa: 0.0 ± 0.0
Gln
3.113GlnAla: 3.113 ± 0.492
0.488GlnCys: 0.488 ± 0.155
1.77GlnAsp: 1.77 ± 0.271
2.259GlnGlu: 2.259 ± 0.364
1.709GlnPhe: 1.709 ± 0.321
2.259GlnGly: 2.259 ± 0.388
0.855GlnHis: 0.855 ± 0.242
2.747GlnIle: 2.747 ± 0.439
1.77GlnLys: 1.77 ± 0.287
4.639GlnLeu: 4.639 ± 0.354
1.709GlnMet: 1.709 ± 0.37
0.977GlnAsn: 0.977 ± 0.218
1.831GlnPro: 1.831 ± 0.362
2.075GlnGln: 2.075 ± 0.404
1.709GlnArg: 1.709 ± 0.267
1.892GlnSer: 1.892 ± 0.23
2.136GlnThr: 2.136 ± 0.38
2.564GlnVal: 2.564 ± 0.423
0.916GlnTrp: 0.916 ± 0.239
1.953GlnTyr: 1.953 ± 0.258
0.0GlnXaa: 0.0 ± 0.0
Arg
3.663ArgAla: 3.663 ± 0.501
0.488ArgCys: 0.488 ± 0.156
1.892ArgAsp: 1.892 ± 0.404
3.113ArgGlu: 3.113 ± 0.487
1.526ArgPhe: 1.526 ± 0.234
2.808ArgGly: 2.808 ± 0.391
1.099ArgHis: 1.099 ± 0.222
3.296ArgIle: 3.296 ± 0.461
3.602ArgLys: 3.602 ± 0.466
3.357ArgLeu: 3.357 ± 0.482
1.648ArgMet: 1.648 ± 0.302
2.014ArgAsn: 2.014 ± 0.362
1.892ArgPro: 1.892 ± 0.375
2.075ArgGln: 2.075 ± 0.389
3.052ArgArg: 3.052 ± 0.457
2.136ArgSer: 2.136 ± 0.402
2.442ArgThr: 2.442 ± 0.305
3.602ArgVal: 3.602 ± 0.387
0.916ArgTrp: 0.916 ± 0.213
1.282ArgTyr: 1.282 ± 0.282
0.0ArgXaa: 0.0 ± 0.0
Ser
5.128SerAla: 5.128 ± 0.732
0.733SerCys: 0.733 ± 0.214
3.785SerAsp: 3.785 ± 0.547
3.174SerGlu: 3.174 ± 0.411
2.381SerPhe: 2.381 ± 0.328
4.7SerGly: 4.7 ± 0.429
0.549SerHis: 0.549 ± 0.178
3.357SerIle: 3.357 ± 0.477
3.235SerLys: 3.235 ± 0.523
3.602SerLeu: 3.602 ± 0.584
1.404SerMet: 1.404 ± 0.276
2.442SerAsn: 2.442 ± 0.407
1.831SerPro: 1.831 ± 0.376
1.587SerGln: 1.587 ± 0.325
2.503SerArg: 2.503 ± 0.395
4.517SerSer: 4.517 ± 0.545
3.846SerThr: 3.846 ± 0.479
4.395SerVal: 4.395 ± 0.601
0.488SerTrp: 0.488 ± 0.206
2.136SerTyr: 2.136 ± 0.386
0.0SerXaa: 0.0 ± 0.0
Thr
6.898ThrAla: 6.898 ± 0.779
0.427ThrCys: 0.427 ± 0.149
4.761ThrAsp: 4.761 ± 0.619
2.991ThrGlu: 2.991 ± 0.486
1.892ThrPhe: 1.892 ± 0.29
5.372ThrGly: 5.372 ± 0.58
1.16ThrHis: 1.16 ± 0.277
3.785ThrIle: 3.785 ± 0.698
3.479ThrLys: 3.479 ± 0.443
4.822ThrLeu: 4.822 ± 0.574
1.587ThrMet: 1.587 ± 0.298
3.602ThrAsn: 3.602 ± 0.402
3.052ThrPro: 3.052 ± 0.408
2.32ThrGln: 2.32 ± 0.36
2.808ThrArg: 2.808 ± 0.46
3.785ThrSer: 3.785 ± 0.444
3.907ThrThr: 3.907 ± 0.702
6.226ThrVal: 6.226 ± 1.169
1.221ThrTrp: 1.221 ± 0.282
2.32ThrTyr: 2.32 ± 0.314
0.0ThrXaa: 0.0 ± 0.0
Val
5.433ValAla: 5.433 ± 0.413
0.733ValCys: 0.733 ± 0.219
6.226ValAsp: 6.226 ± 0.606
4.029ValGlu: 4.029 ± 0.552
1.953ValPhe: 1.953 ± 0.27
5.311ValGly: 5.311 ± 0.585
1.465ValHis: 1.465 ± 0.328
3.602ValIle: 3.602 ± 0.479
4.517ValLys: 4.517 ± 0.531
5.677ValLeu: 5.677 ± 0.695
1.892ValMet: 1.892 ± 0.316
4.517ValAsn: 4.517 ± 0.536
3.357ValPro: 3.357 ± 0.448
3.052ValGln: 3.052 ± 0.45
2.075ValArg: 2.075 ± 0.291
3.602ValSer: 3.602 ± 0.522
6.287ValThr: 6.287 ± 1.273
5.738ValVal: 5.738 ± 0.767
0.916ValTrp: 0.916 ± 0.22
2.136ValTyr: 2.136 ± 0.345
0.0ValXaa: 0.0 ± 0.0
Trp
1.038TrpAla: 1.038 ± 0.266
0.183TrpCys: 0.183 ± 0.088
1.282TrpAsp: 1.282 ± 0.243
0.733TrpGlu: 0.733 ± 0.22
1.038TrpPhe: 1.038 ± 0.229
1.099TrpGly: 1.099 ± 0.211
0.244TrpHis: 0.244 ± 0.134
0.916TrpIle: 0.916 ± 0.196
0.733TrpLys: 0.733 ± 0.204
1.343TrpLeu: 1.343 ± 0.287
0.305TrpMet: 0.305 ± 0.127
0.488TrpAsn: 0.488 ± 0.159
0.671TrpPro: 0.671 ± 0.166
0.366TrpGln: 0.366 ± 0.12
0.733TrpArg: 0.733 ± 0.193
1.282TrpSer: 1.282 ± 0.285
0.733TrpThr: 0.733 ± 0.26
1.648TrpVal: 1.648 ± 0.296
0.427TrpTrp: 0.427 ± 0.166
1.099TrpTyr: 1.099 ± 0.256
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.831TyrAla: 1.831 ± 0.322
0.488TyrCys: 0.488 ± 0.158
3.174TyrAsp: 3.174 ± 0.497
2.136TyrGlu: 2.136 ± 0.388
0.916TyrPhe: 0.916 ± 0.243
2.564TyrGly: 2.564 ± 0.383
0.794TyrHis: 0.794 ± 0.223
2.259TyrIle: 2.259 ± 0.387
2.075TyrLys: 2.075 ± 0.332
2.442TyrLeu: 2.442 ± 0.401
0.733TyrMet: 0.733 ± 0.219
1.831TyrAsn: 1.831 ± 0.325
1.526TyrPro: 1.526 ± 0.301
1.77TyrGln: 1.77 ± 0.314
1.77TyrArg: 1.77 ± 0.302
1.831TyrSer: 1.831 ± 0.317
1.953TyrThr: 1.953 ± 0.342
2.747TyrVal: 2.747 ± 0.38
0.305TyrTrp: 0.305 ± 0.165
1.526TyrTyr: 1.526 ± 0.308
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (16383 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski