Drosophila grimshawi (Hawaiian fruit fly) (Idiomyia grimshawi)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha;

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14754 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B4JX35|B4JX35_DROGR GH17645 OS=Drosophila grimshawi OX=7222 GN=Dgri\GH17645 PE=4 SV=1
MM1 pKa = 7.25NRR3 pKa = 11.84GAEE6 pKa = 4.3RR7 pKa = 11.84KK8 pKa = 10.17GSTANANYY16 pKa = 10.58LIFNFIFVFIVSIFWCIFCFVCRR39 pKa = 11.84VGGGGGGGVCFGFPFCSSTNGTCALKK65 pKa = 10.62SSHH68 pKa = 7.07HH69 pKa = 7.31DD70 pKa = 3.52DD71 pKa = 5.6DD72 pKa = 6.35DD73 pKa = 4.94NHH75 pKa = 6.92DD76 pKa = 4.16QDD78 pKa = 6.3DD79 pKa = 5.06DD80 pKa = 5.64DD81 pKa = 7.04DD82 pKa = 5.96DD83 pKa = 5.13YY84 pKa = 12.28NSDD87 pKa = 4.62DD88 pKa = 5.12AVNDD92 pKa = 4.34DD93 pKa = 5.59DD94 pKa = 7.01DD95 pKa = 4.61DD96 pKa = 3.99

Molecular weight: 10.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B4JE77|B4JE77_DROGR GH11328 OS=Drosophila grimshawi OX=7222 GN=Dgri\GH11328 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.38FLILFILLIAAVSAVPQFGFGRR24 pKa = 11.84PRR26 pKa = 11.84FGGGFRR32 pKa = 11.84KK33 pKa = 10.36GGFRR37 pKa = 11.84RR38 pKa = 11.84GGGGGGGSSSASASASASAAGNGASSAAASASAASGGGRR77 pKa = 11.84FRR79 pKa = 11.84GG80 pKa = 3.6

Molecular weight: 7.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14394

360

14754

7386485

38

8844

500.6

55.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.823 ± 0.025

1.939 ± 0.02

5.178 ± 0.016

6.115 ± 0.026

3.413 ± 0.015

5.702 ± 0.026

2.755 ± 0.012

4.942 ± 0.017

5.353 ± 0.022

9.271 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.399 ± 0.01

5.024 ± 0.016

5.152 ± 0.021

5.775 ± 0.033

5.416 ± 0.017

8.095 ± 0.027

5.877 ± 0.023

5.71 ± 0.015

0.988 ± 0.008

3.033 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski