Atopobium rimae ATCC 49626
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1548 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B9CK31|B9CK31_9ACTN Primosomal protein N' OS=Atopobium rimae ATCC 49626 OX=553184 GN=priA PE=3 SV=1
MM1 pKa = 7.41 KK2 pKa = 9.93 PIIVSIDD9 pKa = 3.39 DD10 pKa = 3.53 RR11 pKa = 11.84 LANPGEE17 pKa = 4.01 ALPYY21 pKa = 10.26 AGHH24 pKa = 7.28 LDD26 pKa = 3.66 EE27 pKa = 5.46 DD28 pKa = 5.0 TYY30 pKa = 11.78 TLGEE34 pKa = 4.37 HH35 pKa = 5.95 TFSLPKK41 pKa = 10.73 GIDD44 pKa = 3.32 YY45 pKa = 11.2 DD46 pKa = 4.71 LVLTNAGEE54 pKa = 4.76 GILVTGILKK63 pKa = 9.96 AHH65 pKa = 5.89 VVGEE69 pKa = 4.14 CDD71 pKa = 3.03 RR72 pKa = 11.84 CLEE75 pKa = 4.22 AATMDD80 pKa = 3.32 IAAEE84 pKa = 3.81 VDD86 pKa = 3.42 EE87 pKa = 5.11 YY88 pKa = 11.81 YY89 pKa = 11.05 LFEE92 pKa = 4.39 EE93 pKa = 5.55 PINPADD99 pKa = 4.99 DD100 pKa = 4.16 EE101 pKa = 5.19 DD102 pKa = 3.98 EE103 pKa = 4.57 ADD105 pKa = 3.71 YY106 pKa = 11.71 SLVSSDD112 pKa = 3.47 RR113 pKa = 11.84 TIDD116 pKa = 3.66 LADD119 pKa = 4.07 ALLPSLVMEE128 pKa = 4.51 TPFVVLCKK136 pKa = 9.68 PDD138 pKa = 3.87 CKK140 pKa = 10.68 GLCPVCGANLNEE152 pKa = 4.48 GDD154 pKa = 4.43 CEE156 pKa = 4.01 HH157 pKa = 6.67 VAQLEE162 pKa = 4.5 HH163 pKa = 6.81 KK164 pKa = 10.1 RR165 pKa = 11.84 SEE167 pKa = 4.41 DD168 pKa = 3.09 ARR170 pKa = 11.84 AANPFSVLANLTFDD184 pKa = 3.77 EE185 pKa = 4.87 EE186 pKa = 4.42 EE187 pKa = 3.98
Molecular weight: 20.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.961
IPC_protein 3.948
Toseland 3.745
ProMoST 4.062
Dawson 3.923
Bjellqvist 4.101
Wikipedia 3.821
Rodwell 3.783
Grimsley 3.656
Solomon 3.91
Lehninger 3.872
Nozaki 4.037
DTASelect 4.228
Thurlkill 3.783
EMBOSS 3.834
Sillero 4.062
Patrickios 1.214
IPC_peptide 3.91
IPC2_peptide 4.05
IPC2.peptide.svr19 3.965
Protein with the highest isoelectric point:
>tr|B9CLS7|B9CLS7_9ACTN DNA gyrase subunit A OS=Atopobium rimae ATCC 49626 OX=553184 GN=gyrA PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 8.9 RR10 pKa = 11.84 KK11 pKa = 9.76 RR12 pKa = 11.84 ATTHH16 pKa = 5.7 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.3 NGRR28 pKa = 11.84 AVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.11 GRR39 pKa = 11.84 KK40 pKa = 9.03 RR41 pKa = 11.84 LTVV44 pKa = 3.11
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1548
0
1548
485172
37
1589
313.4
34.42
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.871 ± 0.073
1.379 ± 0.026
6.045 ± 0.049
6.497 ± 0.06
3.865 ± 0.047
7.451 ± 0.054
2.213 ± 0.029
6.072 ± 0.042
4.92 ± 0.054
9.322 ± 0.066
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.529 ± 0.029
3.388 ± 0.036
3.941 ± 0.036
3.252 ± 0.03
5.261 ± 0.052
6.46 ± 0.052
5.907 ± 0.053
7.671 ± 0.059
1.015 ± 0.024
2.941 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here