Salmonella phage Felix O1 (isolate Felix O1-VT1) (Bacteriophage Felix O1)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Ounavirinae; Felixounavirus; Salmonella virus FelixO1

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 247 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6KGD7|Q6KGD7_BPFO1 DNA ligase OS=Salmonella phage Felix O1 (isolate Felix O1-VT1) OX=1283336 PE=4 SV=1
MM1 pKa = 7.67NITSNIKK8 pKa = 10.43LMNDD12 pKa = 3.05ALNSVSPTINANQLTFSWDD31 pKa = 2.94DD32 pKa = 3.94WITLSYY38 pKa = 10.51GCSGVNNIITVDD50 pKa = 3.02IVYY53 pKa = 10.79

Molecular weight: 5.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6KGP3|Q6KGP3_BPFO1 Uncharacterized protein OS=Salmonella phage Felix O1 (isolate Felix O1-VT1) OX=1283336 PE=4 SV=1
MM1 pKa = 7.28NRR3 pKa = 11.84PLFTDD8 pKa = 4.11SIGDD12 pKa = 3.61SSTSSTVNRR21 pKa = 11.84VYY23 pKa = 10.35TLSPLEE29 pKa = 4.16TTLFPTMRR37 pKa = 11.84TRR39 pKa = 11.84EE40 pKa = 4.16PLRR43 pKa = 11.84VYY45 pKa = 10.85LKK47 pKa = 10.51VPKK50 pKa = 10.37SLAQSFIVFSLEE62 pKa = 3.72NKK64 pKa = 9.28KK65 pKa = 10.68GSRR68 pKa = 11.84RR69 pKa = 11.84SLL71 pKa = 3.32

Molecular weight: 8.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

246

1

247

37266

37

909

150.9

17.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.518 ± 0.247

1.798 ± 0.119

5.531 ± 0.165

6.22 ± 0.203

4.629 ± 0.137

5.796 ± 0.188

2.249 ± 0.128

6.217 ± 0.126

7.747 ± 0.198

8.769 ± 0.193

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.895 ± 0.12

5.171 ± 0.15

3.097 ± 0.123

3.443 ± 0.116

4.251 ± 0.134

7.17 ± 0.226

6.268 ± 0.218

7.031 ± 0.148

1.165 ± 0.082

4.036 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski