Enterococcus durans
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3031 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2A7SPF5|A0A2A7SPF5_9ENTE Uncharacterized protein OS=Enterococcus durans OX=53345 GN=EA71_02361 PE=4 SV=1
MM1 pKa = 7.13 EE2 pKa = 5.49 KK3 pKa = 10.37 EE4 pKa = 4.06 YY5 pKa = 10.59 FVQQEE10 pKa = 4.36 AFMEE14 pKa = 5.08 DD15 pKa = 4.06 YY16 pKa = 10.75 QQLAAQKK23 pKa = 10.2 DD24 pKa = 3.86 VTIEE28 pKa = 3.97 SLADD32 pKa = 3.79 KK33 pKa = 10.47 IINQLNQTQEE43 pKa = 4.29 DD44 pKa = 5.7 YY45 pKa = 11.8 YY46 pKa = 11.25 EE47 pKa = 4.44 MPAEE51 pKa = 3.96 EE52 pKa = 4.73 AKK54 pKa = 11.01 DD55 pKa = 4.0 GVSHH59 pKa = 7.22 RR60 pKa = 11.84 FPFSRR65 pKa = 11.84 RR66 pKa = 11.84 IYY68 pKa = 10.54 SDD70 pKa = 3.82 EE71 pKa = 4.08 NDD73 pKa = 3.36 GTLNTEE79 pKa = 3.84 YY80 pKa = 9.75 TYY82 pKa = 10.95 EE83 pKa = 4.31 GEE85 pKa = 4.14 PYY87 pKa = 10.24 ILQTEE92 pKa = 4.29 DD93 pKa = 3.75 DD94 pKa = 4.04 FF95 pKa = 7.43
Molecular weight: 11.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.794
IPC2_protein 3.986
IPC_protein 3.91
Toseland 3.732
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.77
Rodwell 3.745
Grimsley 3.643
Solomon 3.859
Lehninger 3.821
Nozaki 3.986
DTASelect 4.139
Thurlkill 3.77
EMBOSS 3.783
Sillero 4.024
Patrickios 1.888
IPC_peptide 3.859
IPC2_peptide 4.012
IPC2.peptide.svr19 3.909
Protein with the highest isoelectric point:
>tr|A0A2A7SQY2|A0A2A7SQY2_9ENTE Replicative DNA helicase OS=Enterococcus durans OX=53345 GN=dnaC PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 9.16 RR10 pKa = 11.84 KK11 pKa = 9.12 RR12 pKa = 11.84 QKK14 pKa = 9.38 VHH16 pKa = 5.8 GFRR19 pKa = 11.84 KK20 pKa = 10.04 RR21 pKa = 11.84 MSTKK25 pKa = 9.46 NGRR28 pKa = 11.84 RR29 pKa = 11.84 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.73 GRR39 pKa = 11.84 KK40 pKa = 8.76 VLSAA44 pKa = 4.05
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.434
IPC2_protein 11.14
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.427
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.149
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.052
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3031
0
3031
869336
31
2147
286.8
32.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.806 ± 0.043
0.612 ± 0.011
5.181 ± 0.035
7.431 ± 0.055
4.622 ± 0.038
6.359 ± 0.046
1.869 ± 0.022
7.732 ± 0.045
7.243 ± 0.047
9.945 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.606 ± 0.024
4.636 ± 0.035
3.424 ± 0.024
4.264 ± 0.035
3.885 ± 0.03
5.997 ± 0.033
5.982 ± 0.044
6.673 ± 0.038
0.947 ± 0.014
3.784 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here