Perca flavescens (American yellow perch) (Morone flavescens)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei; Eurypterygia; Ctenosquamata;

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21644 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A484CST4|A0A484CST4_PERFV Uncharacterized protein OS=Perca flavescens OX=8167 GN=EPR50_G00139000 PE=4 SV=1
MM1 pKa = 7.49VDD3 pKa = 3.99FGEE6 pKa = 4.19ILTTIGDD13 pKa = 3.52FGFFQKK19 pKa = 10.94LILFGLTLPNVLLPVFFCSFLFIQSDD45 pKa = 4.09PEE47 pKa = 4.12RR48 pKa = 11.84HH49 pKa = 6.65CNTDD53 pKa = 3.05WILQADD59 pKa = 4.25SNLTTDD65 pKa = 3.81DD66 pKa = 4.02QLNLTLPRR74 pKa = 11.84EE75 pKa = 4.14EE76 pKa = 5.45DD77 pKa = 3.65GTFSRR82 pKa = 11.84CQMFVPVDD90 pKa = 3.33WDD92 pKa = 3.4VGDD95 pKa = 3.42IRR97 pKa = 11.84EE98 pKa = 4.18FGLNEE103 pKa = 3.75TTGCQNGWVYY113 pKa = 11.55YY114 pKa = 8.15NTLYY118 pKa = 10.7EE119 pKa = 4.13ATIVTDD125 pKa = 3.52VSEE128 pKa = 4.34DD129 pKa = 3.21AA130 pKa = 4.9

Molecular weight: 14.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A484DFF3|A0A484DFF3_PERFV Guanine nucleotide-binding protein-like 3 OS=Perca flavescens OX=8167 GN=EPR50_G00041740 PE=4 SV=1
MM1 pKa = 7.44GFLRR5 pKa = 11.84RR6 pKa = 11.84VAGVSLRR13 pKa = 11.84DD14 pKa = 3.38RR15 pKa = 11.84RR16 pKa = 11.84PQQRR20 pKa = 11.84RR21 pKa = 11.84KK22 pKa = 9.82QKK24 pKa = 10.56PNTPSTKK31 pKa = 10.11RR32 pKa = 11.84LTSQHH37 pKa = 5.78SAQGTSEE44 pKa = 4.44SKK46 pKa = 10.7AHH48 pKa = 6.44PLSPAEE54 pKa = 4.96DD55 pKa = 3.3KK56 pKa = 10.79MEE58 pKa = 4.86PKK60 pKa = 9.97VQPDD64 pKa = 3.82TQPCSHH70 pKa = 7.13RR71 pKa = 11.84EE72 pKa = 3.86HH73 pKa = 7.07KK74 pKa = 9.14PTGFRR79 pKa = 11.84VSRR82 pKa = 11.84HH83 pKa = 4.12TAAFSCHH90 pKa = 6.27RR91 pKa = 11.84LSDD94 pKa = 3.83SSPTLQEE101 pKa = 3.99SLSPKK106 pKa = 10.52AEE108 pKa = 3.94VGGLAGHH115 pKa = 6.82GEE117 pKa = 4.3GDD119 pKa = 3.45SDD121 pKa = 3.97TDD123 pKa = 3.6LSEE126 pKa = 4.44SEE128 pKa = 4.6RR129 pKa = 11.84LPVSPSGGVPPQLQLRR145 pKa = 11.84PEE147 pKa = 4.38VIEE150 pKa = 5.39AEE152 pKa = 4.31HH153 pKa = 6.55CPSRR157 pKa = 11.84SHH159 pKa = 7.25RR160 pKa = 11.84PRR162 pKa = 11.84GHH164 pKa = 5.57NHH166 pKa = 6.04GAFDD170 pKa = 4.99FPDD173 pKa = 4.44FLPPPFNSWSLSQLAVFYY191 pKa = 11.14NMEE194 pKa = 4.17GRR196 pKa = 11.84GGPRR200 pKa = 11.84PRR202 pKa = 11.84PMGPLEE208 pKa = 4.54RR209 pKa = 11.84YY210 pKa = 9.59LDD212 pKa = 3.76RR213 pKa = 11.84LLQLEE218 pKa = 4.24WHH220 pKa = 6.59QIQTVQGEE228 pKa = 4.66GRR230 pKa = 11.84KK231 pKa = 10.24SMGSDD236 pKa = 3.23VLSSCHH242 pKa = 6.44RR243 pKa = 11.84SHH245 pKa = 7.17AAASSRR251 pKa = 11.84LSSPKK256 pKa = 10.31CILQCQRR263 pKa = 11.84AFSLTFLSCLASHH276 pKa = 6.78SALLSGCACTLCRR289 pKa = 11.84IRR291 pKa = 11.84YY292 pKa = 5.9STCSTLCCRR301 pKa = 11.84SSHH304 pKa = 5.75HH305 pKa = 5.63THH307 pKa = 6.9HH308 pKa = 6.74SRR310 pKa = 11.84LSPMLEE316 pKa = 3.57RR317 pKa = 11.84RR318 pKa = 11.84GPTLLPKK325 pKa = 10.34RR326 pKa = 11.84SYY328 pKa = 11.11SEE330 pKa = 4.16NRR332 pKa = 11.84VKK334 pKa = 10.96SSDD337 pKa = 3.33RR338 pKa = 11.84SSAPRR343 pKa = 11.84SQVFSSPVRR352 pKa = 11.84TNSHH356 pKa = 6.07LRR358 pKa = 11.84RR359 pKa = 11.84MQASGNIRR367 pKa = 11.84NPVKK371 pKa = 10.49VANTKK376 pKa = 9.13PHH378 pKa = 4.9STARR382 pKa = 11.84GGSVGAGTDD391 pKa = 3.1RR392 pKa = 11.84VGALGDD398 pKa = 3.63VVDD401 pKa = 4.1YY402 pKa = 8.86RR403 pKa = 11.84TGGFRR408 pKa = 11.84RR409 pKa = 11.84RR410 pKa = 11.84SGSEE414 pKa = 3.34QRR416 pKa = 11.84RR417 pKa = 11.84GGVEE421 pKa = 3.85RR422 pKa = 11.84QQGASEE428 pKa = 4.02KK429 pKa = 9.77RR430 pKa = 11.84RR431 pKa = 11.84SGSEE435 pKa = 3.59YY436 pKa = 9.86RR437 pKa = 11.84RR438 pKa = 11.84GGAEE442 pKa = 3.63RR443 pKa = 11.84KK444 pKa = 9.85RR445 pKa = 11.84IAEE448 pKa = 4.0LKK450 pKa = 9.8EE451 pKa = 4.31RR452 pKa = 11.84EE453 pKa = 4.23IKK455 pKa = 10.48PDD457 pKa = 2.95VTAIMDD463 pKa = 4.04NLPGPKK469 pKa = 9.19HH470 pKa = 6.54SPINRR475 pKa = 11.84PNRR478 pKa = 11.84QKK480 pKa = 10.57QVEE483 pKa = 4.56FVTT486 pKa = 4.2

Molecular weight: 53.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21644

0

21644

12322852

50

22184

569.3

63.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.692 ± 0.015

2.209 ± 0.015

5.232 ± 0.011

6.965 ± 0.023

3.461 ± 0.014

6.452 ± 0.022

2.628 ± 0.009

4.24 ± 0.013

5.578 ± 0.02

9.463 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.392 ± 0.007

3.79 ± 0.01

5.808 ± 0.022

4.825 ± 0.02

5.697 ± 0.014

8.775 ± 0.024

5.664 ± 0.013

6.318 ± 0.013

1.141 ± 0.006

2.67 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski