Corynebacterium sp. 366
Average proteome isoelectric point is 5.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1880 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6I0FEE4|A0A6I0FEE4_9CORY Uncharacterized protein OS=Corynebacterium sp. 366 OX=2652251 GN=F8390_06140 PE=4 SV=1
MM1 pKa = 8.37 IEE3 pKa = 3.99 EE4 pKa = 4.38 LKK6 pKa = 10.93 SVGLAFPRR14 pKa = 11.84 WQDD17 pKa = 3.37 ALEE20 pKa = 4.01 TAYY23 pKa = 11.03 SSGQLFVSGEE33 pKa = 3.93 VRR35 pKa = 11.84 GGQVLQYY42 pKa = 10.86 DD43 pKa = 4.31 DD44 pKa = 4.98 ASGARR49 pKa = 11.84 LVVMAVEE56 pKa = 4.22 PFGTFASYY64 pKa = 10.53 FGGVSTSAHH73 pKa = 5.03 VSMVDD78 pKa = 4.34 DD79 pKa = 4.83 IIGVIDD85 pKa = 4.03 VVDD88 pKa = 5.06 DD89 pKa = 4.23 SPALQTSGQQAPVVASVAATIAQGPMLSDD118 pKa = 4.0 EE119 pKa = 5.1 PPLEE123 pKa = 4.05 YY124 pKa = 10.59 QQVQVSALATSVEE137 pKa = 4.41 VFTDD141 pKa = 3.94 PSMLTAAGITGTPGTIEE158 pKa = 3.99 ATGLTDD164 pKa = 4.58 LNSGSTSPHH173 pKa = 5.7 ASASIVVEE181 pKa = 4.14 TSAVGRR187 pKa = 11.84 RR188 pKa = 11.84 TNQLTGQQFWACSVHH203 pKa = 7.32 APFDD207 pKa = 3.77 FTLLIPADD215 pKa = 3.76 VADD218 pKa = 4.13 IASLAIDD225 pKa = 3.91 DD226 pKa = 4.73 AGAPRR231 pKa = 11.84 SLIIAGQVQFTATVVDD247 pKa = 4.23 APGCGGSGGCGSGGCGCGGHH267 pKa = 6.68
Molecular weight: 27.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.91
IPC_protein 3.897
Toseland 3.681
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.821
Rodwell 3.719
Grimsley 3.592
Solomon 3.872
Lehninger 3.834
Nozaki 3.999
DTASelect 4.24
Thurlkill 3.732
EMBOSS 3.834
Sillero 4.012
Patrickios 1.074
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.893
Protein with the highest isoelectric point:
>tr|A0A6I0FMC1|A0A6I0FMC1_9CORY DNA helicase OS=Corynebacterium sp. 366 OX=2652251 GN=F8390_02930 PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 VHH19 pKa = 5.99 GFRR22 pKa = 11.84 TRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVSARR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 9.22 GRR42 pKa = 11.84 KK43 pKa = 9.07 SLTAA47 pKa = 4.07
Molecular weight: 5.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.345
IPC_protein 12.954
Toseland 13.115
ProMoST 13.612
Dawson 13.115
Bjellqvist 13.115
Wikipedia 13.598
Rodwell 12.735
Grimsley 13.159
Solomon 13.612
Lehninger 13.51
Nozaki 13.115
DTASelect 13.115
Thurlkill 13.115
EMBOSS 13.612
Sillero 13.115
Patrickios 12.457
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.26
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1880
0
1880
638581
24
1680
339.7
36.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.42 ± 0.075
0.74 ± 0.014
6.072 ± 0.046
6.319 ± 0.057
3.197 ± 0.032
8.354 ± 0.051
2.354 ± 0.025
4.93 ± 0.042
3.343 ± 0.05
9.33 ± 0.066
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.309 ± 0.027
2.905 ± 0.039
5.004 ± 0.04
3.611 ± 0.032
6.153 ± 0.055
6.12 ± 0.044
6.123 ± 0.037
8.209 ± 0.05
1.371 ± 0.023
2.138 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here