Gordonia phage DatBoi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bantamvirus; unclassified Bantamvirus

Average proteome isoelectric point is 5.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 166 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6XL73|A0A6G6XL73_9CAUD Uncharacterized protein OS=Gordonia phage DatBoi OX=2704037 GN=122 PE=4 SV=1
MM1 pKa = 7.56SIVIEE6 pKa = 4.08QVEE9 pKa = 4.24TSRR12 pKa = 11.84WYY14 pKa = 10.47VDD16 pKa = 3.28EE17 pKa = 6.25DD18 pKa = 3.6EE19 pKa = 4.83LAEE22 pKa = 4.25LVAYY26 pKa = 9.18WGEE29 pKa = 4.22EE30 pKa = 4.08YY31 pKa = 10.43PEE33 pKa = 5.47LSTDD37 pKa = 3.59MQPDD41 pKa = 3.37ALLAFLDD48 pKa = 3.99DD49 pKa = 3.75QFDD52 pKa = 3.67RR53 pKa = 11.84GSDD56 pKa = 3.24VAYY59 pKa = 9.25WIQEE63 pKa = 3.53NWINRR68 pKa = 11.84TDD70 pKa = 3.21AYY72 pKa = 9.97EE73 pKa = 3.81AHH75 pKa = 5.58TTITHH80 pKa = 6.47EE81 pKa = 4.43GAA83 pKa = 3.57

Molecular weight: 9.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6XLK2|A0A6G6XLK2_9CAUD Uncharacterized protein OS=Gordonia phage DatBoi OX=2704037 GN=71 PE=4 SV=1
MM1 pKa = 7.83RR2 pKa = 11.84KK3 pKa = 8.51HH4 pKa = 5.49HH5 pKa = 6.17TRR7 pKa = 11.84PHH9 pKa = 6.16AARR12 pKa = 11.84GRR14 pKa = 11.84YY15 pKa = 6.83VTCEE19 pKa = 3.83CGKK22 pKa = 10.13RR23 pKa = 11.84AYY25 pKa = 7.47EE26 pKa = 3.93TRR28 pKa = 11.84KK29 pKa = 9.87DD30 pKa = 3.51AKK32 pKa = 10.38LSRR35 pKa = 11.84GTMGGSAGTGMAAYY49 pKa = 10.02RR50 pKa = 11.84CDD52 pKa = 3.72LAPASMPWHH61 pKa = 6.74LGHH64 pKa = 7.33LNKK67 pKa = 9.94KK68 pKa = 8.91SKK70 pKa = 10.35IYY72 pKa = 10.68GRR74 pKa = 11.84LL75 pKa = 3.25

Molecular weight: 8.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

166

0

166

28465

30

2945

171.5

18.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.01 ± 0.446

0.871 ± 0.118

7.377 ± 0.24

7.058 ± 0.226

2.969 ± 0.134

8.038 ± 0.286

2.094 ± 0.147

5.196 ± 0.163

3.618 ± 0.194

7.954 ± 0.181

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.48 ± 0.096

3.183 ± 0.166

4.88 ± 0.165

3.218 ± 0.274

7.247 ± 0.239

5.185 ± 0.159

6.011 ± 0.149

6.766 ± 0.244

1.981 ± 0.138

2.863 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski