Amino acid dipepetide frequency for Gordonia phage DatBoi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.912AlaAla: 13.912 ± 1.276
0.843AlaCys: 0.843 ± 0.21
8.08AlaAsp: 8.08 ± 0.487
8.467AlaGlu: 8.467 ± 0.741
2.811AlaPhe: 2.811 ± 0.317
9.064AlaGly: 9.064 ± 0.681
1.546AlaHis: 1.546 ± 0.226
5.516AlaIle: 5.516 ± 0.373
4.462AlaLys: 4.462 ± 0.485
9.767AlaLeu: 9.767 ± 0.75
3.408AlaMet: 3.408 ± 0.323
3.197AlaAsn: 3.197 ± 0.429
4.602AlaPro: 4.602 ± 0.411
4.251AlaGln: 4.251 ± 0.685
7.167AlaArg: 7.167 ± 0.526
4.708AlaSer: 4.708 ± 0.398
6.71AlaThr: 6.71 ± 0.823
6.64AlaVal: 6.64 ± 0.463
2.003AlaTrp: 2.003 ± 0.25
2.951AlaTyr: 2.951 ± 0.389
0.0AlaXaa: 0.0 ± 0.0
Cys
0.773CysAla: 0.773 ± 0.199
0.105CysCys: 0.105 ± 0.06
0.703CysAsp: 0.703 ± 0.159
0.562CysGlu: 0.562 ± 0.161
0.246CysPhe: 0.246 ± 0.121
1.335CysGly: 1.335 ± 0.293
0.351CysHis: 0.351 ± 0.119
0.351CysIle: 0.351 ± 0.127
0.246CysLys: 0.246 ± 0.096
0.492CysLeu: 0.492 ± 0.156
0.281CysMet: 0.281 ± 0.131
0.211CysAsn: 0.211 ± 0.098
0.492CysPro: 0.492 ± 0.139
0.211CysGln: 0.211 ± 0.092
0.738CysArg: 0.738 ± 0.216
0.386CysSer: 0.386 ± 0.131
0.281CysThr: 0.281 ± 0.104
0.527CysVal: 0.527 ± 0.15
0.176CysTrp: 0.176 ± 0.083
0.246CysTyr: 0.246 ± 0.094
0.0CysXaa: 0.0 ± 0.0
Asp
7.272AspAla: 7.272 ± 0.563
0.843AspCys: 0.843 ± 0.189
4.954AspAsp: 4.954 ± 0.567
6.254AspGlu: 6.254 ± 0.635
2.354AspPhe: 2.354 ± 0.334
6.008AspGly: 6.008 ± 0.384
1.757AspHis: 1.757 ± 0.296
3.97AspIle: 3.97 ± 0.417
2.354AspLys: 2.354 ± 0.342
6.394AspLeu: 6.394 ± 0.43
1.651AspMet: 1.651 ± 0.233
2.53AspAsn: 2.53 ± 0.299
4.146AspPro: 4.146 ± 0.361
1.862AspGln: 1.862 ± 0.257
5.481AspArg: 5.481 ± 0.438
3.127AspSer: 3.127 ± 0.322
3.654AspThr: 3.654 ± 0.339
5.27AspVal: 5.27 ± 0.524
1.827AspTrp: 1.827 ± 0.286
2.073AspTyr: 2.073 ± 0.304
0.0AspXaa: 0.0 ± 0.0
Glu
7.834GluAla: 7.834 ± 0.751
0.562GluCys: 0.562 ± 0.18
4.778GluAsp: 4.778 ± 0.446
4.321GluGlu: 4.321 ± 0.431
2.846GluPhe: 2.846 ± 0.353
4.708GluGly: 4.708 ± 0.577
1.405GluHis: 1.405 ± 0.23
4.146GluIle: 4.146 ± 0.467
2.284GluLys: 2.284 ± 0.244
6.57GluLeu: 6.57 ± 0.549
1.476GluMet: 1.476 ± 0.242
2.53GluAsn: 2.53 ± 0.305
2.811GluPro: 2.811 ± 0.406
3.127GluGln: 3.127 ± 0.331
4.883GluArg: 4.883 ± 0.465
3.408GluSer: 3.408 ± 0.416
3.865GluThr: 3.865 ± 0.409
4.918GluVal: 4.918 ± 0.375
1.651GluTrp: 1.651 ± 0.214
2.459GluTyr: 2.459 ± 0.348
0.0GluXaa: 0.0 ± 0.0
Phe
3.408PheAla: 3.408 ± 0.351
0.422PheCys: 0.422 ± 0.145
2.916PheAsp: 2.916 ± 0.319
2.003PheGlu: 2.003 ± 0.237
0.632PhePhe: 0.632 ± 0.144
2.846PheGly: 2.846 ± 0.378
0.632PheHis: 0.632 ± 0.189
1.019PheIle: 1.019 ± 0.235
0.808PheLys: 0.808 ± 0.141
1.757PheLeu: 1.757 ± 0.315
0.878PheMet: 0.878 ± 0.178
1.3PheAsn: 1.3 ± 0.207
1.054PhePro: 1.054 ± 0.168
0.668PheGln: 0.668 ± 0.134
2.319PheArg: 2.319 ± 0.293
1.616PheSer: 1.616 ± 0.224
2.354PheThr: 2.354 ± 0.311
1.721PheVal: 1.721 ± 0.285
0.632PheTrp: 0.632 ± 0.174
0.703PheTyr: 0.703 ± 0.142
0.0PheXaa: 0.0 ± 0.0
Gly
7.413GlyAla: 7.413 ± 0.605
0.668GlyCys: 0.668 ± 0.171
5.937GlyAsp: 5.937 ± 0.788
5.972GlyGlu: 5.972 ± 0.517
2.881GlyPhe: 2.881 ± 0.337
7.764GlyGly: 7.764 ± 1.014
2.108GlyHis: 2.108 ± 0.268
3.338GlyIle: 3.338 ± 0.475
3.162GlyLys: 3.162 ± 0.325
6.183GlyLeu: 6.183 ± 0.47
1.897GlyMet: 1.897 ± 0.244
2.319GlyAsn: 2.319 ± 0.382
3.373GlyPro: 3.373 ± 0.369
3.056GlyGln: 3.056 ± 0.444
5.621GlyArg: 5.621 ± 0.472
4.286GlySer: 4.286 ± 0.431
4.567GlyThr: 4.567 ± 0.487
5.41GlyVal: 5.41 ± 0.477
1.651GlyTrp: 1.651 ± 0.225
2.775GlyTyr: 2.775 ± 0.263
0.0GlyXaa: 0.0 ± 0.0
His
1.792HisAla: 1.792 ± 0.309
0.246HisCys: 0.246 ± 0.098
1.476HisAsp: 1.476 ± 0.261
1.3HisGlu: 1.3 ± 0.221
0.808HisPhe: 0.808 ± 0.197
1.265HisGly: 1.265 ± 0.194
0.316HisHis: 0.316 ± 0.105
1.265HisIle: 1.265 ± 0.205
0.562HisLys: 0.562 ± 0.18
2.389HisLeu: 2.389 ± 0.229
0.386HisMet: 0.386 ± 0.123
0.632HisAsn: 0.632 ± 0.169
1.44HisPro: 1.44 ± 0.25
0.773HisGln: 0.773 ± 0.144
1.476HisArg: 1.476 ± 0.224
1.019HisSer: 1.019 ± 0.222
1.23HisThr: 1.23 ± 0.23
1.335HisVal: 1.335 ± 0.194
0.422HisTrp: 0.422 ± 0.115
0.808HisTyr: 0.808 ± 0.175
0.0HisXaa: 0.0 ± 0.0
Ile
6.008IleAla: 6.008 ± 0.464
0.527IleCys: 0.527 ± 0.14
5.375IleAsp: 5.375 ± 0.44
4.918IleGlu: 4.918 ± 0.487
1.335IlePhe: 1.335 ± 0.198
3.267IleGly: 3.267 ± 0.424
1.124IleHis: 1.124 ± 0.22
2.635IleIle: 2.635 ± 0.329
1.862IleLys: 1.862 ± 0.279
3.162IleLeu: 3.162 ± 0.314
0.949IleMet: 0.949 ± 0.136
1.686IleAsn: 1.686 ± 0.285
2.389IlePro: 2.389 ± 0.265
1.476IleGln: 1.476 ± 0.244
4.181IleArg: 4.181 ± 0.316
2.178IleSer: 2.178 ± 0.26
2.916IleThr: 2.916 ± 0.286
3.302IleVal: 3.302 ± 0.461
0.984IleTrp: 0.984 ± 0.223
1.686IleTyr: 1.686 ± 0.252
0.0IleXaa: 0.0 ± 0.0
Lys
4.673LysAla: 4.673 ± 0.763
0.141LysCys: 0.141 ± 0.072
2.073LysAsp: 2.073 ± 0.242
1.476LysGlu: 1.476 ± 0.23
0.843LysPhe: 0.843 ± 0.17
2.775LysGly: 2.775 ± 0.449
0.632LysHis: 0.632 ± 0.16
2.53LysIle: 2.53 ± 0.239
1.757LysLys: 1.757 ± 0.304
3.056LysLeu: 3.056 ± 0.41
0.984LysMet: 0.984 ± 0.153
1.019LysAsn: 1.019 ± 0.202
2.284LysPro: 2.284 ± 0.302
0.913LysGln: 0.913 ± 0.151
2.811LysArg: 2.811 ± 0.362
1.827LysSer: 1.827 ± 0.281
2.108LysThr: 2.108 ± 0.299
2.284LysVal: 2.284 ± 0.357
0.738LysTrp: 0.738 ± 0.161
1.265LysTyr: 1.265 ± 0.2
0.0LysXaa: 0.0 ± 0.0
Leu
8.713LeuAla: 8.713 ± 0.484
0.843LeuCys: 0.843 ± 0.189
7.167LeuAsp: 7.167 ± 0.462
4.813LeuGlu: 4.813 ± 0.362
1.967LeuPhe: 1.967 ± 0.264
5.691LeuGly: 5.691 ± 0.519
1.721LeuHis: 1.721 ± 0.286
4.392LeuIle: 4.392 ± 0.356
2.6LeuLys: 2.6 ± 0.274
5.797LeuLeu: 5.797 ± 0.468
1.546LeuMet: 1.546 ± 0.248
2.846LeuAsn: 2.846 ± 0.335
4.216LeuPro: 4.216 ± 0.355
2.635LeuGln: 2.635 ± 0.42
6.183LeuArg: 6.183 ± 0.502
4.989LeuSer: 4.989 ± 0.461
4.813LeuThr: 4.813 ± 0.525
5.305LeuVal: 5.305 ± 0.531
1.335LeuTrp: 1.335 ± 0.236
1.967LeuTyr: 1.967 ± 0.247
0.0LeuXaa: 0.0 ± 0.0
Met
3.408MetAla: 3.408 ± 0.328
0.211MetCys: 0.211 ± 0.1
1.265MetAsp: 1.265 ± 0.227
1.159MetGlu: 1.159 ± 0.246
0.843MetPhe: 0.843 ± 0.167
1.546MetGly: 1.546 ± 0.217
0.668MetHis: 0.668 ± 0.183
0.878MetIle: 0.878 ± 0.164
1.019MetLys: 1.019 ± 0.171
1.546MetLeu: 1.546 ± 0.205
0.422MetMet: 0.422 ± 0.11
0.773MetAsn: 0.773 ± 0.195
1.405MetPro: 1.405 ± 0.221
0.878MetGln: 0.878 ± 0.144
1.476MetArg: 1.476 ± 0.289
2.53MetSer: 2.53 ± 0.27
2.354MetThr: 2.354 ± 0.26
1.721MetVal: 1.721 ± 0.252
0.281MetTrp: 0.281 ± 0.079
0.422MetTyr: 0.422 ± 0.122
0.0MetXaa: 0.0 ± 0.0
Asn
3.443AsnAla: 3.443 ± 0.485
0.105AsnCys: 0.105 ± 0.07
2.213AsnAsp: 2.213 ± 0.276
1.616AsnGlu: 1.616 ± 0.261
0.808AsnPhe: 0.808 ± 0.142
2.986AsnGly: 2.986 ± 0.413
0.527AsnHis: 0.527 ± 0.158
1.581AsnIle: 1.581 ± 0.231
1.054AsnLys: 1.054 ± 0.181
2.986AsnLeu: 2.986 ± 0.346
0.843AsnMet: 0.843 ± 0.2
0.808AsnAsn: 0.808 ± 0.183
2.53AsnPro: 2.53 ± 0.331
1.019AsnGln: 1.019 ± 0.192
2.635AsnArg: 2.635 ± 0.288
1.476AsnSer: 1.476 ± 0.285
1.757AsnThr: 1.757 ± 0.279
1.792AsnVal: 1.792 ± 0.263
0.668AsnTrp: 0.668 ± 0.159
0.949AsnTyr: 0.949 ± 0.187
0.0AsnXaa: 0.0 ± 0.0
Pro
5.762ProAla: 5.762 ± 0.473
0.492ProCys: 0.492 ± 0.141
3.829ProAsp: 3.829 ± 0.339
3.583ProGlu: 3.583 ± 0.365
1.194ProPhe: 1.194 ± 0.181
5.129ProGly: 5.129 ± 0.451
1.159ProHis: 1.159 ± 0.224
2.038ProIle: 2.038 ± 0.273
1.862ProLys: 1.862 ± 0.305
3.021ProLeu: 3.021 ± 0.345
1.23ProMet: 1.23 ± 0.211
1.792ProAsn: 1.792 ± 0.244
2.108ProPro: 2.108 ± 0.354
1.124ProGln: 1.124 ± 0.246
2.635ProArg: 2.635 ± 0.337
2.67ProSer: 2.67 ± 0.301
3.232ProThr: 3.232 ± 0.362
3.829ProVal: 3.829 ± 0.403
0.949ProTrp: 0.949 ± 0.174
1.159ProTyr: 1.159 ± 0.215
0.0ProXaa: 0.0 ± 0.0
Gln
3.724GlnAla: 3.724 ± 0.748
0.281GlnCys: 0.281 ± 0.107
1.546GlnAsp: 1.546 ± 0.25
2.038GlnGlu: 2.038 ± 0.377
1.265GlnPhe: 1.265 ± 0.243
1.932GlnGly: 1.932 ± 0.287
0.527GlnHis: 0.527 ± 0.145
2.178GlnIle: 2.178 ± 0.327
1.124GlnLys: 1.124 ± 0.26
3.162GlnLeu: 3.162 ± 0.638
0.878GlnMet: 0.878 ± 0.184
0.457GlnAsn: 0.457 ± 0.12
1.405GlnPro: 1.405 ± 0.208
1.37GlnGln: 1.37 ± 0.279
2.459GlnArg: 2.459 ± 0.29
1.37GlnSer: 1.37 ± 0.258
1.721GlnThr: 1.721 ± 0.236
2.811GlnVal: 2.811 ± 0.328
0.808GlnTrp: 0.808 ± 0.179
1.124GlnTyr: 1.124 ± 0.209
0.0GlnXaa: 0.0 ± 0.0
Arg
7.167ArgAla: 7.167 ± 0.556
0.492ArgCys: 0.492 ± 0.149
4.567ArgAsp: 4.567 ± 0.371
6.148ArgGlu: 6.148 ± 0.507
2.284ArgPhe: 2.284 ± 0.267
5.27ArgGly: 5.27 ± 0.371
1.897ArgHis: 1.897 ± 0.247
3.583ArgIle: 3.583 ± 0.368
3.443ArgLys: 3.443 ± 0.367
5.691ArgLeu: 5.691 ± 0.478
2.073ArgMet: 2.073 ± 0.259
2.635ArgAsn: 2.635 ± 0.306
2.916ArgPro: 2.916 ± 0.413
2.881ArgGln: 2.881 ± 0.422
5.797ArgArg: 5.797 ± 0.546
3.267ArgSer: 3.267 ± 0.373
3.97ArgThr: 3.97 ± 0.344
4.954ArgVal: 4.954 ± 0.411
1.37ArgTrp: 1.37 ± 0.214
2.073ArgTyr: 2.073 ± 0.284
0.0ArgXaa: 0.0 ± 0.0
Ser
5.164SerAla: 5.164 ± 0.395
0.386SerCys: 0.386 ± 0.128
3.619SerAsp: 3.619 ± 0.322
2.916SerGlu: 2.916 ± 0.339
1.3SerPhe: 1.3 ± 0.246
4.813SerGly: 4.813 ± 0.517
0.738SerHis: 0.738 ± 0.198
2.916SerIle: 2.916 ± 0.343
2.213SerLys: 2.213 ± 0.237
4.427SerLeu: 4.427 ± 0.407
1.37SerMet: 1.37 ± 0.291
1.335SerAsn: 1.335 ± 0.222
2.951SerPro: 2.951 ± 0.268
1.37SerGln: 1.37 ± 0.273
3.583SerArg: 3.583 ± 0.408
2.986SerSer: 2.986 ± 0.314
3.302SerThr: 3.302 ± 0.346
3.513SerVal: 3.513 ± 0.315
1.124SerTrp: 1.124 ± 0.232
1.827SerTyr: 1.827 ± 0.232
0.0SerXaa: 0.0 ± 0.0
Thr
6.183ThrAla: 6.183 ± 0.539
0.457ThrCys: 0.457 ± 0.148
4.146ThrAsp: 4.146 ± 0.316
3.9ThrGlu: 3.9 ± 0.339
2.213ThrPhe: 2.213 ± 0.303
5.481ThrGly: 5.481 ± 0.468
1.335ThrHis: 1.335 ± 0.204
3.408ThrIle: 3.408 ± 0.348
2.073ThrLys: 2.073 ± 0.275
4.392ThrLeu: 4.392 ± 0.375
1.511ThrMet: 1.511 ± 0.228
1.827ThrAsn: 1.827 ± 0.316
3.9ThrPro: 3.9 ± 0.384
1.476ThrGln: 1.476 ± 0.317
4.321ThrArg: 4.321 ± 0.445
3.056ThrSer: 3.056 ± 0.347
4.427ThrThr: 4.427 ± 0.401
3.724ThrVal: 3.724 ± 0.385
0.984ThrTrp: 0.984 ± 0.201
1.3ThrTyr: 1.3 ± 0.202
0.0ThrXaa: 0.0 ± 0.0
Val
8.186ValAla: 8.186 ± 0.575
0.562ValCys: 0.562 ± 0.166
5.235ValAsp: 5.235 ± 0.455
5.762ValGlu: 5.762 ± 0.554
1.757ValPhe: 1.757 ± 0.266
4.883ValGly: 4.883 ± 0.447
1.405ValHis: 1.405 ± 0.269
3.794ValIle: 3.794 ± 0.46
2.143ValLys: 2.143 ± 0.252
5.129ValLeu: 5.129 ± 0.47
1.476ValMet: 1.476 ± 0.253
2.284ValAsn: 2.284 ± 0.281
2.846ValPro: 2.846 ± 0.34
1.581ValGln: 1.581 ± 0.237
4.778ValArg: 4.778 ± 0.501
3.97ValSer: 3.97 ± 0.403
4.356ValThr: 4.356 ± 0.386
4.602ValVal: 4.602 ± 0.454
1.194ValTrp: 1.194 ± 0.266
1.721ValTyr: 1.721 ± 0.285
0.0ValXaa: 0.0 ± 0.0
Trp
2.038TrpAla: 2.038 ± 0.305
0.211TrpCys: 0.211 ± 0.102
1.721TrpAsp: 1.721 ± 0.248
1.23TrpGlu: 1.23 ± 0.271
0.527TrpPhe: 0.527 ± 0.122
1.23TrpGly: 1.23 ± 0.234
0.527TrpHis: 0.527 ± 0.117
1.37TrpIle: 1.37 ± 0.247
0.422TrpLys: 0.422 ± 0.152
1.335TrpLeu: 1.335 ± 0.207
0.703TrpMet: 0.703 ± 0.165
0.632TrpAsn: 0.632 ± 0.171
0.668TrpPro: 0.668 ± 0.145
0.597TrpGln: 0.597 ± 0.147
1.827TrpArg: 1.827 ± 0.348
1.511TrpSer: 1.511 ± 0.259
0.984TrpThr: 0.984 ± 0.197
1.546TrpVal: 1.546 ± 0.221
0.386TrpTrp: 0.386 ± 0.11
0.351TrpTyr: 0.351 ± 0.106
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.232TyrAla: 3.232 ± 0.375
0.316TyrCys: 0.316 ± 0.096
2.178TyrAsp: 2.178 ± 0.344
2.143TyrGlu: 2.143 ± 0.371
0.773TyrPhe: 0.773 ± 0.162
2.389TyrGly: 2.389 ± 0.287
0.562TyrHis: 0.562 ± 0.156
1.054TyrIle: 1.054 ± 0.175
0.738TyrLys: 0.738 ± 0.151
2.284TyrLeu: 2.284 ± 0.302
0.808TyrMet: 0.808 ± 0.183
1.019TyrAsn: 1.019 ± 0.23
1.265TyrPro: 1.265 ± 0.228
0.913TyrGln: 0.913 ± 0.14
2.108TyrArg: 2.108 ± 0.28
1.476TyrSer: 1.476 ± 0.255
1.511TyrThr: 1.511 ± 0.273
2.459TyrVal: 2.459 ± 0.364
0.632TyrTrp: 0.632 ± 0.145
0.773TyrTyr: 0.773 ± 0.175
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 166 proteins (28465 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski