Legionella massiliensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Legionellaceae; Legionella

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3750 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A078KX31|A0A078KX31_9GAMM D-alanyl-D-alanine carboxypeptidase DacC OS=Legionella massiliensis OX=1034943 GN=dacC PE=3 SV=1
MM1 pKa = 7.91AILPDD6 pKa = 4.27VINKK10 pKa = 9.42NLVDD14 pKa = 3.44MDD16 pKa = 4.51EE17 pKa = 4.97IEE19 pKa = 4.39FTLHH23 pKa = 6.61SLLGSDD29 pKa = 3.16TGVFIGISTNDD40 pKa = 3.58YY41 pKa = 11.5GEE43 pKa = 5.78LIQQNGHH50 pKa = 6.05LNEE53 pKa = 3.69INAYY57 pKa = 9.46IGADD61 pKa = 3.42NALSTASGRR70 pKa = 11.84IVYY73 pKa = 9.85QLGLQGPNLAIDD85 pKa = 4.23SACSSSFDD93 pKa = 3.62MYY95 pKa = 11.53CNFIKK100 pKa = 10.58FNKK103 pKa = 9.7FSFSKK108 pKa = 10.66EE109 pKa = 3.82EE110 pKa = 4.15PEE112 pKa = 5.5LISQAGDD119 pKa = 3.14TNAEE123 pKa = 4.05QEE125 pKa = 4.47LMPGALYY132 pKa = 10.8ADD134 pKa = 4.52DD135 pKa = 4.06

Molecular weight: 14.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A078KT09|A0A078KT09_9GAMM Cytoplasmic glycerophosphodiester phosphodiesterase OS=Legionella massiliensis OX=1034943 GN=BN59_00463 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.18RR12 pKa = 11.84KK13 pKa = 8.83RR14 pKa = 11.84DD15 pKa = 3.29HH16 pKa = 7.17GFRR19 pKa = 11.84QRR21 pKa = 11.84MATRR25 pKa = 11.84GGRR28 pKa = 11.84LVLKK32 pKa = 10.32RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.84GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3750

0

3750

1263423

29

3807

336.9

37.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.24 ± 0.045

1.154 ± 0.017

4.935 ± 0.032

6.109 ± 0.049

4.419 ± 0.03

5.96 ± 0.042

2.275 ± 0.019

6.939 ± 0.03

5.937 ± 0.042

11.191 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.229 ± 0.02

4.819 ± 0.033

4.165 ± 0.025

4.757 ± 0.033

4.411 ± 0.031

6.907 ± 0.034

5.174 ± 0.047

5.838 ± 0.035

1.123 ± 0.015

3.416 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski