Rathayibacter tritici

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Rathayibacter

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2806 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A160KRR5|A0A160KRR5_9MICO Metal-dependent phosphoesterase OS=Rathayibacter tritici OX=33888 GN=A6122_0846 PE=4 SV=1
MM1 pKa = 8.05RR2 pKa = 11.84LRR4 pKa = 11.84SSAAAAILALALAADD19 pKa = 4.01VSGCASGAVIDD30 pKa = 4.74IPDD33 pKa = 3.81SAVSASATNVPPATSAPATAEE54 pKa = 3.76QDD56 pKa = 3.46VDD58 pKa = 3.89CAGALAATAIEE69 pKa = 4.27QDD71 pKa = 3.18TGLPAGSVVLAAAGDD86 pKa = 3.84TCSYY90 pKa = 11.28ALAGNDD96 pKa = 3.41SAVVLTLHH104 pKa = 7.18RR105 pKa = 11.84GPLLEE110 pKa = 4.34TFTGAGEE117 pKa = 3.95AAGAVPVSLGTAAYY131 pKa = 7.17WLPGLAPAPSEE142 pKa = 4.01LAVLAGGWEE151 pKa = 3.9LRR153 pKa = 11.84IVSSVGEE160 pKa = 4.06QATLVDD166 pKa = 3.82WAVSALASVGVPLAVAA182 pKa = 4.49

Molecular weight: 17.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A160KWQ6|A0A160KWQ6_9MICO NikQ protein OS=Rathayibacter tritici OX=33888 GN=A6122_2769 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.56GRR40 pKa = 11.84TEE42 pKa = 4.14LSAA45 pKa = 4.86

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2806

0

2806

922148

36

6430

328.6

35.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.473 ± 0.061

0.547 ± 0.011

6.053 ± 0.043

5.771 ± 0.039

2.942 ± 0.029

9.048 ± 0.04

1.936 ± 0.023

4.094 ± 0.038

1.787 ± 0.034

10.475 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.529 ± 0.016

1.792 ± 0.027

5.424 ± 0.031

2.637 ± 0.025

7.756 ± 0.055

6.23 ± 0.033

6.09 ± 0.044

9.138 ± 0.047

1.374 ± 0.019

1.903 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski