Bauldia litoralis
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4760 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G6AVP0|A0A1G6AVP0_9RHIZ ATP-dependent Clp protease adapter protein ClpS OS=Bauldia litoralis OX=665467 GN=clpS PE=3 SV=1
MM1 pKa = 7.99 IIRR4 pKa = 11.84 FCAALLAAASLSGCFTSDD22 pKa = 3.35 APLIDD27 pKa = 4.24 PEE29 pKa = 4.0 EE30 pKa = 3.91 AAFPFEE36 pKa = 4.31 TIVYY40 pKa = 8.92 QGEE43 pKa = 4.26 GRR45 pKa = 11.84 DD46 pKa = 3.75 EE47 pKa = 4.2 VQTLIRR53 pKa = 11.84 KK54 pKa = 8.69 GDD56 pKa = 3.56 AYY58 pKa = 11.04 VVEE61 pKa = 4.45 PADD64 pKa = 2.86 IEE66 pKa = 4.16 MRR68 pKa = 11.84 VRR70 pKa = 11.84 FLAVGDD76 pKa = 3.84 DD77 pKa = 4.0 TYY79 pKa = 11.89 VALAEE84 pKa = 4.67 GSDD87 pKa = 3.74 GDD89 pKa = 4.12 EE90 pKa = 4.03 TAVLYY95 pKa = 9.49 TFLKK99 pKa = 10.36 VDD101 pKa = 4.56 LAAMTAEE108 pKa = 4.95 SFASMAPEE116 pKa = 4.89 DD117 pKa = 4.88 GIDD120 pKa = 3.31 RR121 pKa = 11.84 PGFSDD126 pKa = 3.49 CGDD129 pKa = 3.55 RR130 pKa = 11.84 GTCVDD135 pKa = 5.52 DD136 pKa = 3.95 LDD138 pKa = 6.04 AYY140 pKa = 9.66 IAYY143 pKa = 9.59 AQARR147 pKa = 11.84 VAAGDD152 pKa = 4.0 LPDD155 pKa = 3.44 IVYY158 pKa = 9.14 TIISAEE164 pKa = 3.81
Molecular weight: 17.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.745
IPC_protein 3.732
Toseland 3.516
ProMoST 3.884
Dawson 3.732
Bjellqvist 3.923
Wikipedia 3.668
Rodwell 3.567
Grimsley 3.427
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.088
Thurlkill 3.579
EMBOSS 3.681
Sillero 3.859
Patrickios 1.863
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.788
Protein with the highest isoelectric point:
>tr|A0A1G6E6I8|A0A1G6E6I8_9RHIZ Asp/Glu/Hydantoin racemase OS=Bauldia litoralis OX=665467 GN=SAMN02982931_04197 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 9.84 LVRR12 pKa = 11.84 KK13 pKa = 9.15 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATTGGRR28 pKa = 11.84 KK29 pKa = 9.29 VIAARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.03 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4760
0
4760
1523614
39
2283
320.1
34.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.752 ± 0.05
0.752 ± 0.01
6.331 ± 0.032
5.667 ± 0.031
3.825 ± 0.023
9.029 ± 0.042
1.889 ± 0.014
5.489 ± 0.025
2.906 ± 0.026
9.623 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.385 ± 0.016
2.491 ± 0.017
5.258 ± 0.027
2.581 ± 0.016
6.951 ± 0.042
5.176 ± 0.023
5.49 ± 0.02
7.866 ± 0.028
1.327 ± 0.014
2.212 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here