Psychromonas sp. B3M02
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3376 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A366WD60|A0A366WD60_9GAMM Exodeoxyribonuclease III OS=Psychromonas sp. B3M02 OX=2267226 GN=DS885_00805 PE=3 SV=1
MM1 pKa = 7.45 LLIAGYY7 pKa = 9.87 LLPIAVNASNLDD19 pKa = 3.83 LATKK23 pKa = 9.96 PLYY26 pKa = 10.16 TGTSEE31 pKa = 4.52 APMMMLTMGRR41 pKa = 11.84 DD42 pKa = 2.96 HH43 pKa = 6.59 TLFYY47 pKa = 10.59 EE48 pKa = 4.94 AYY50 pKa = 10.01 NDD52 pKa = 4.77 ASDD55 pKa = 4.66 LDD57 pKa = 4.27 GDD59 pKa = 4.63 GEE61 pKa = 5.12 LDD63 pKa = 3.48 TTFKK67 pKa = 10.67 PDD69 pKa = 3.32 YY70 pKa = 9.25 TYY72 pKa = 11.23 EE73 pKa = 4.5 GYY75 pKa = 10.56 FDD77 pKa = 3.64 STACYY82 pKa = 9.92 KK83 pKa = 10.46 YY84 pKa = 10.33 RR85 pKa = 11.84 SNKK88 pKa = 9.68 SRR90 pKa = 11.84 FKK92 pKa = 10.44 PKK94 pKa = 9.95 TIEE97 pKa = 4.31 TVCSNNNHH105 pKa = 5.99 WSGNFLNYY113 pKa = 9.65 LTMTRR118 pKa = 11.84 MDD120 pKa = 3.85 VLRR123 pKa = 11.84 KK124 pKa = 9.6 VLYY127 pKa = 10.55 GGTRR131 pKa = 11.84 STDD134 pKa = 3.59 DD135 pKa = 3.43 EE136 pKa = 5.34 DD137 pKa = 3.57 EE138 pKa = 4.8 TILEE142 pKa = 4.32 RR143 pKa = 11.84 VFIPQDD149 pKa = 2.91 AHH151 pKa = 6.55 SWAKK155 pKa = 10.39 AYY157 pKa = 9.72 TSVEE161 pKa = 3.54 VDD163 pKa = 3.86 GYY165 pKa = 11.09 DD166 pKa = 3.12 ISDD169 pKa = 3.66 YY170 pKa = 10.97 TPYY173 pKa = 11.04 SLPDD177 pKa = 3.02 SGKK180 pKa = 7.96 QHH182 pKa = 6.61 FFGTVTYY189 pKa = 10.33 SGLGEE194 pKa = 4.27 DD195 pKa = 3.92 PILRR199 pKa = 11.84 VRR201 pKa = 11.84 EE202 pKa = 4.14 NISYY206 pKa = 10.89 SSDD209 pKa = 3.22 DD210 pKa = 3.18 WGVWSWASTEE220 pKa = 4.27 RR221 pKa = 11.84 PVLDD225 pKa = 4.32 ASGDD229 pKa = 3.79 DD230 pKa = 3.35 YY231 pKa = 11.61 VVRR234 pKa = 11.84 VEE236 pKa = 4.02 ACVDD240 pKa = 3.56 GFTDD244 pKa = 5.16 GNNCDD249 pKa = 4.87 LYY251 pKa = 10.7 PSGNYY256 pKa = 9.47 KK257 pKa = 8.81 PTGLLHH263 pKa = 7.69 DD264 pKa = 4.59 YY265 pKa = 11.58 GEE267 pKa = 4.38 TDD269 pKa = 3.12 QMLFGLLTGSYY280 pKa = 10.35 NKK282 pKa = 9.97 NLSGGVLRR290 pKa = 11.84 RR291 pKa = 11.84 AISEE295 pKa = 3.89 FSEE298 pKa = 4.57 EE299 pKa = 4.05 VDD301 pKa = 3.11 SDD303 pKa = 3.64 TGQFTSTEE311 pKa = 4.07 DD312 pKa = 4.05 GIVATIDD319 pKa = 3.08 KK320 pKa = 10.69 LRR322 pKa = 11.84 IHH324 pKa = 7.1 GFNYY328 pKa = 10.2 SNKK331 pKa = 9.49 TYY333 pKa = 9.63 ATNCGWVVNGAISDD347 pKa = 4.11 GQCASWGNPAGEE359 pKa = 4.16 MLYY362 pKa = 10.76 EE363 pKa = 4.12 AMRR366 pKa = 11.84 YY367 pKa = 8.7 YY368 pKa = 10.84 SGVEE372 pKa = 4.05 SASPSYY378 pKa = 9.71 ATSGSYY384 pKa = 10.16 DD385 pKa = 3.57 AEE387 pKa = 4.44 VGLTSPDD394 pKa = 2.98 WDD396 pKa = 4.68 DD397 pKa = 3.81 PFEE400 pKa = 5.48 DD401 pKa = 4.88 RR402 pKa = 11.84 GTCANPNVLLISDD415 pKa = 4.59 ISPSYY420 pKa = 11.17 DD421 pKa = 3.22 SDD423 pKa = 3.68 QLPGVYY429 pKa = 9.94 SEE431 pKa = 4.58 FTKK434 pKa = 10.82 SANNDD439 pKa = 2.9 EE440 pKa = 5.37 AYY442 pKa = 10.44 DD443 pKa = 3.89 GTTLPGFSVSEE454 pKa = 3.9 LLEE457 pKa = 4.51 TISTSEE463 pKa = 4.34 GKK465 pKa = 8.93 TSGQFFIGQSGTDD478 pKa = 3.24 NDD480 pKa = 4.48 GAPTAKK486 pKa = 10.43 DD487 pKa = 3.04 ITGLEE492 pKa = 4.4 SVRR495 pKa = 11.84 GLAPAEE501 pKa = 3.98 PTKK504 pKa = 10.68 KK505 pKa = 10.45 GSYY508 pKa = 9.86 SSAAVAYY515 pKa = 9.07 YY516 pKa = 8.81 GHH518 pKa = 7.27 RR519 pKa = 11.84 NDD521 pKa = 3.03 IHH523 pKa = 5.61 LTKK526 pKa = 10.95 DD527 pKa = 2.61 EE528 pKa = 4.5 DD529 pKa = 4.0 QNVTTMVVALASTLPEE545 pKa = 4.17 IEE547 pKa = 3.98 VDD549 pKa = 3.29 VDD551 pKa = 3.84 GEE553 pKa = 4.44 VIKK556 pKa = 10.51 IVPFAKK562 pKa = 10.2 SVGWGNKK569 pKa = 8.22 ISADD573 pKa = 3.49 EE574 pKa = 4.98 GDD576 pKa = 4.08 FQPTNSIIDD585 pKa = 3.56 WYY587 pKa = 10.26 IEE589 pKa = 4.34 SITDD593 pKa = 3.27 TKK595 pKa = 11.23 GVFRR599 pKa = 11.84 INYY602 pKa = 8.76 EE603 pKa = 4.03 DD604 pKa = 3.82 VEE606 pKa = 4.42 QGADD610 pKa = 3.14 HH611 pKa = 7.22 DD612 pKa = 3.7 MDD614 pKa = 4.61 MIVKK618 pKa = 8.05 YY619 pKa = 9.03 TYY621 pKa = 9.01 EE622 pKa = 4.08 VKK624 pKa = 10.66 EE625 pKa = 4.49 GLCKK629 pKa = 10.18 TYY631 pKa = 10.9 DD632 pKa = 3.43 SDD634 pKa = 4.76 GDD636 pKa = 3.91 CTEE639 pKa = 3.95 TAKK642 pKa = 10.84 GVEE645 pKa = 4.0 ITLDD649 pKa = 3.23 STYY652 pKa = 11.27 AAGGVDD658 pKa = 3.06 QHH660 pKa = 7.59 AGYY663 pKa = 9.94 IISGTTNDD671 pKa = 3.45 GMYY674 pKa = 10.77 LEE676 pKa = 4.83 VKK678 pKa = 10.06 DD679 pKa = 4.47 VSGANIKK686 pKa = 10.67 YY687 pKa = 10.64 YY688 pKa = 10.87 LDD690 pKa = 4.02 TPDD693 pKa = 3.09 STVYY697 pKa = 10.87 DD698 pKa = 3.56 NRR700 pKa = 11.84 DD701 pKa = 3.03 ISGSTSNLGLYY712 pKa = 7.44 ATRR715 pKa = 11.84 NFFPSDD721 pKa = 2.95 EE722 pKa = 4.33 SAADD726 pKa = 3.89 FLPSPLWYY734 pKa = 9.32 AAKK737 pKa = 9.96 WGGFTDD743 pKa = 3.53 SDD745 pKa = 4.4 GDD747 pKa = 3.82 GTPNLDD753 pKa = 4.55 SEE755 pKa = 4.7 WDD757 pKa = 3.39 TSGADD762 pKa = 4.07 DD763 pKa = 4.97 GGADD767 pKa = 4.45 GDD769 pKa = 4.06 PDD771 pKa = 3.65 NYY773 pKa = 10.67 FPVTNAGEE781 pKa = 4.66 LKK783 pKa = 10.51 DD784 pKa = 3.73 QLAAAFDD791 pKa = 3.92 LAYY794 pKa = 10.72 DD795 pKa = 4.05 GLLTGASATYY805 pKa = 10.45 SSNLLSSGSLAYY817 pKa = 10.15 QSSFEE822 pKa = 4.1 EE823 pKa = 4.54 EE824 pKa = 3.99 TWNGDD829 pKa = 3.07 VNAYY833 pKa = 9.03 QVSSDD838 pKa = 3.73 GEE840 pKa = 4.41 YY841 pKa = 10.95 ASNATWSAATQLDD854 pKa = 4.29 AMDD857 pKa = 5.26 PDD859 pKa = 3.43 NRR861 pKa = 11.84 HH862 pKa = 5.95 IYY864 pKa = 8.58 TRR866 pKa = 11.84 SNEE869 pKa = 3.9 SSLLGSQGAVFEE881 pKa = 4.61 FTTSAISTTLLSSLTDD897 pKa = 3.28 SLTSVSTSTLTDD909 pKa = 3.36 SFSEE913 pKa = 4.22 SQLFSLLGDD922 pKa = 3.63 VTGSTTEE929 pKa = 3.69 KK930 pKa = 10.43 LAYY933 pKa = 7.68 LTNVVGYY940 pKa = 10.31 IRR942 pKa = 11.84 GDD944 pKa = 3.25 RR945 pKa = 11.84 DD946 pKa = 3.58 NEE948 pKa = 4.06 VTTVVDD954 pKa = 4.14 GFRR957 pKa = 11.84 EE958 pKa = 4.03 RR959 pKa = 11.84 SSVLGDD965 pKa = 3.34 IVNASPYY972 pKa = 9.22 YY973 pKa = 9.54 VSSAKK978 pKa = 10.52 DD979 pKa = 3.11 HH980 pKa = 5.72 TVATEE985 pKa = 4.14 VVAFAANDD993 pKa = 3.77 GMVHH997 pKa = 6.77 IVDD1000 pKa = 4.21 ADD1002 pKa = 3.86 DD1003 pKa = 4.34 GKK1005 pKa = 11.18 EE1006 pKa = 3.59 LAAYY1010 pKa = 8.88 IPSGVYY1016 pKa = 9.81 DD1017 pKa = 3.68 ALGNLVDD1024 pKa = 3.36 SAYY1027 pKa = 9.35 IHH1029 pKa = 6.86 EE1030 pKa = 4.28 YY1031 pKa = 10.74 SVDD1034 pKa = 3.5 GGLNAYY1040 pKa = 10.3 SDD1042 pKa = 4.44 EE1043 pKa = 4.3 NDD1045 pKa = 5.32 LIDD1048 pKa = 3.6 VDD1050 pKa = 4.03 INGVSTEE1057 pKa = 4.05 VARR1060 pKa = 11.84 TTLVGRR1066 pKa = 11.84 LGLGVKK1072 pKa = 9.81 GLYY1075 pKa = 10.61 AIDD1078 pKa = 3.87 VSDD1081 pKa = 3.92 ISNPTTDD1088 pKa = 2.88 MMRR1091 pKa = 11.84 WEE1093 pKa = 4.47 ITTDD1097 pKa = 2.9 TDD1099 pKa = 3.44 GFEE1102 pKa = 4.36 GLGLSVSEE1110 pKa = 4.02 PTIVEE1115 pKa = 4.2 LANGKK1120 pKa = 10.33 ASVVFANGYY1129 pKa = 10.34 NSTDD1133 pKa = 3.61 DD1134 pKa = 3.44 EE1135 pKa = 4.55 GAIYY1139 pKa = 10.2 IADD1142 pKa = 4.0 LTDD1145 pKa = 3.4 GSLIKK1150 pKa = 10.53 RR1151 pKa = 11.84 LTVGVQTDD1159 pKa = 3.59 PTGEE1163 pKa = 3.99 DD1164 pKa = 3.31 RR1165 pKa = 11.84 PNALAQPLLVDD1176 pKa = 4.01 NNSDD1180 pKa = 3.93 GIADD1184 pKa = 3.69 YY1185 pKa = 10.9 LYY1187 pKa = 11.04 AGDD1190 pKa = 4.98 LFGNMWAFDD1199 pKa = 3.74 ISSEE1203 pKa = 4.74 DD1204 pKa = 3.54 PSEE1207 pKa = 3.82 WGIKK1211 pKa = 8.01 KK1212 pKa = 7.94 TAGAPLFTALSPTLDD1227 pKa = 3.43 NQEE1230 pKa = 4.47 YY1231 pKa = 8.23 MSQPITSRR1239 pKa = 11.84 PSVVRR1244 pKa = 11.84 HH1245 pKa = 5.99 PDD1247 pKa = 3.33 GEE1249 pKa = 4.55 GVLIAFGTGKK1259 pKa = 10.38 YY1260 pKa = 9.73 VEE1262 pKa = 4.92 SDD1264 pKa = 3.3 DD1265 pKa = 5.14 TSADD1269 pKa = 3.68 DD1270 pKa = 3.85 QATQSFYY1277 pKa = 11.39 VVEE1280 pKa = 4.95 DD1281 pKa = 3.71 NLGSAVVTAVRR1292 pKa = 11.84 SSSGFSNLQKK1302 pKa = 10.8 QSITKK1307 pKa = 9.63 EE1308 pKa = 3.87 SSTNRR1313 pKa = 11.84 LLSSNSLNWDD1323 pKa = 4.05 EE1324 pKa = 5.27 ISGFYY1329 pKa = 9.83 IDD1331 pKa = 5.78 LVNTEE1336 pKa = 5.06 DD1337 pKa = 4.87 GNTNNYY1343 pKa = 9.89 GEE1345 pKa = 4.32 RR1346 pKa = 11.84 QVVASTVLGTKK1357 pKa = 10.06 VSFVTLIPGTDD1368 pKa = 3.2 PCVAGGTGWYY1378 pKa = 8.87 MEE1380 pKa = 4.58 LNLYY1384 pKa = 8.27 TGQTWDD1390 pKa = 3.05 TGTEE1394 pKa = 4.1 VEE1396 pKa = 5.18 DD1397 pKa = 4.92 DD1398 pKa = 3.72 PSTTVDD1404 pKa = 3.41 EE1405 pKa = 4.7 SEE1407 pKa = 5.04 EE1408 pKa = 4.13 IPTDD1412 pKa = 3.42 SSNQFLDD1419 pKa = 4.34 GVATGLSSVASVTTSTEE1436 pKa = 4.02 TITNEE1441 pKa = 3.98 DD1442 pKa = 3.6 GTTEE1446 pKa = 3.97 EE1447 pKa = 4.26 VTVFEE1452 pKa = 4.4 SAEE1455 pKa = 3.97 GLTCITLSTNKK1466 pKa = 7.78 QTCFEE1471 pKa = 5.31 DD1472 pKa = 3.79 DD1473 pKa = 4.11 LNITGPVSWRR1483 pKa = 11.84 QLYY1486 pKa = 9.99
Molecular weight: 160.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.709
IPC2_protein 3.859
IPC_protein 3.897
Toseland 3.668
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.808
Rodwell 3.719
Grimsley 3.579
Solomon 3.884
Lehninger 3.834
Nozaki 3.986
DTASelect 4.24
Thurlkill 3.719
EMBOSS 3.821
Sillero 4.012
Patrickios 1.202
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.9
Protein with the highest isoelectric point:
>tr|A0A366W235|A0A366W235_9GAMM DUF1488 domain-containing protein OS=Psychromonas sp. B3M02 OX=2267226 GN=DS885_11835 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNTKK11 pKa = 10.0 RR12 pKa = 11.84 KK13 pKa = 8.73 RR14 pKa = 11.84 SHH16 pKa = 6.16 GFRR19 pKa = 11.84 TRR21 pKa = 11.84 MATKK25 pKa = 10.18 NGRR28 pKa = 11.84 AVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.5 GRR39 pKa = 11.84 ASLSAA44 pKa = 3.83
Molecular weight: 5.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3376
0
3376
1096779
31
2425
324.9
36.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.36 ± 0.043
1.041 ± 0.014
5.554 ± 0.039
6.112 ± 0.043
4.293 ± 0.031
6.418 ± 0.044
2.136 ± 0.022
7.102 ± 0.031
5.751 ± 0.038
10.638 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.446 ± 0.018
4.592 ± 0.033
3.646 ± 0.028
4.773 ± 0.039
3.747 ± 0.026
6.813 ± 0.034
5.597 ± 0.033
6.744 ± 0.036
1.117 ± 0.016
3.122 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here