Ophiocordyceps camponoti-saundersi (nom. inval.)
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6946 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A369HK36|A0A369HK36_9HYPO Uncharacterized protein OS=Ophiocordyceps camponoti-saundersi (nom. inval.) OX=2039874 GN=CP533_5381 PE=3 SV=1
MM1 pKa = 7.7 KK2 pKa = 10.28 YY3 pKa = 10.22 GRR5 pKa = 11.84 AVVGLVLASTGLADD19 pKa = 5.07 EE20 pKa = 5.24 CAQKK24 pKa = 10.87 CNEE27 pKa = 4.41 ANASCLGNADD37 pKa = 4.24 ANQSACASDD46 pKa = 3.8 YY47 pKa = 10.87 AACLGFDD54 pKa = 4.04 PFGSDD59 pKa = 3.62 GSLITPTACAGGAAPTGGPDD79 pKa = 2.96 GGSYY83 pKa = 10.14 IGDD86 pKa = 3.53 EE87 pKa = 4.12 APAAVRR93 pKa = 11.84 LADD96 pKa = 4.55 DD97 pKa = 4.14 GFEE100 pKa = 4.08 ATVAPGLPPVSPVSIPDD117 pKa = 3.43 VAPLITAPLSAAACAKK133 pKa = 10.24 SCEE136 pKa = 4.19 QGYY139 pKa = 9.0 IACGSSPDD147 pKa = 4.3 SSQCDD152 pKa = 3.42 ALYY155 pKa = 10.89 ADD157 pKa = 4.35 CLGYY161 pKa = 11.08 NPVGPDD167 pKa = 3.37 GSLVAPTACSIRR179 pKa = 11.84 GAPTAAPPLVPAPPVVPAPPVVPAPPVVPAPPVVPAPPPVPAPPPVPAPAPVPAPAPVPAPAPVPAPPLVPAPGGAAIAPVVDD262 pKa = 5.49 GIGASPIGGIVEE274 pKa = 4.63 AGGSTPEE281 pKa = 3.9 ACAVSCKK288 pKa = 9.78 NAYY291 pKa = 9.48 SGCTSNSVADD301 pKa = 3.87 QSTCASDD308 pKa = 3.72 YY309 pKa = 10.74 AACLGYY315 pKa = 10.88 DD316 pKa = 3.74 PFNTDD321 pKa = 2.95 GALLTPTACSPGGAAPTPGAPVPGAVLPNGNAYY354 pKa = 9.02 PADD357 pKa = 3.6 GSVPVVVAGSDD368 pKa = 3.42 RR369 pKa = 11.84 AKK371 pKa = 9.53 PAAMAMLAMVLAYY384 pKa = 10.47 LL385 pKa = 4.49
Molecular weight: 36.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.87
IPC2_protein 3.656
IPC_protein 3.668
Toseland 3.427
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.656
Rodwell 3.49
Grimsley 3.338
Solomon 3.668
Lehninger 3.617
Nozaki 3.795
DTASelect 4.088
Thurlkill 3.503
EMBOSS 3.656
Sillero 3.795
Patrickios 0.006
IPC_peptide 3.656
IPC2_peptide 3.77
IPC2.peptide.svr19 3.729
Protein with the highest isoelectric point:
>tr|A0A369HIH9|A0A369HIH9_9HYPO Leucyl-tRNA synthetase OS=Ophiocordyceps camponoti-saundersi (nom. inval.) OX=2039874 GN=CP533_2220 PE=3 SV=1
MM1 pKa = 7.77 KK2 pKa = 10.61 SPDD5 pKa = 4.19 EE6 pKa = 4.36 PPQPKK11 pKa = 9.65 PKK13 pKa = 10.07 NLPWRR18 pKa = 11.84 IAADD22 pKa = 4.14 TISAAAAASSVAPLIAIIDD41 pKa = 3.65 RR42 pKa = 11.84 SIMEE46 pKa = 4.36 KK47 pKa = 10.57 ASGRR51 pKa = 11.84 RR52 pKa = 11.84 PSLWSSVRR60 pKa = 11.84 ASLSSIARR68 pKa = 11.84 RR69 pKa = 11.84 PHH71 pKa = 5.62 TLLFSKK77 pKa = 10.5 PSALIFLVYY86 pKa = 10.58 GGTYY90 pKa = 8.83 LTANAVDD97 pKa = 4.04 TVNSAFVEE105 pKa = 4.16 PRR107 pKa = 11.84 LPPEE111 pKa = 4.47 SVSSGPAKK119 pKa = 10.4 FFASSAANVGLCLVKK134 pKa = 10.71 DD135 pKa = 3.68 RR136 pKa = 11.84 AFVRR140 pKa = 11.84 LFASSTKK147 pKa = 10.34 AAVPAPVPLPCYY159 pKa = 10.7 ALFTLRR165 pKa = 11.84 DD166 pKa = 4.32 CITIFASFNLPPRR179 pKa = 11.84 LAPYY183 pKa = 10.34 LDD185 pKa = 3.72 ARR187 pKa = 11.84 MPPALADD194 pKa = 4.05 FFSGRR199 pKa = 11.84 SAAQFLAPAAVQLLSTPLHH218 pKa = 6.15 LLGLDD223 pKa = 3.95 LYY225 pKa = 10.95 ARR227 pKa = 11.84 PAPTPPSLRR236 pKa = 11.84 WRR238 pKa = 11.84 AVCQNWLSSAAARR251 pKa = 11.84 ICRR254 pKa = 11.84 IVPAFGLGGVINLKK268 pKa = 10.19 LRR270 pKa = 11.84 RR271 pKa = 11.84 HH272 pKa = 5.92 LMLTLEE278 pKa = 4.51 SSSLSTPSSRR288 pKa = 11.84 LASSSS293 pKa = 3.12
Molecular weight: 31.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.648
IPC_protein 10.467
Toseland 10.643
ProMoST 10.365
Dawson 10.745
Bjellqvist 10.482
Wikipedia 10.965
Rodwell 10.935
Grimsley 10.804
Solomon 10.862
Lehninger 10.833
Nozaki 10.657
DTASelect 10.467
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.687
Patrickios 10.643
IPC_peptide 10.877
IPC2_peptide 9.677
IPC2.peptide.svr19 8.553
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6872
74
6946
3813413
21
4997
549.0
60.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.329 ± 0.027
1.304 ± 0.012
6.185 ± 0.024
5.955 ± 0.027
3.485 ± 0.016
6.984 ± 0.034
2.412 ± 0.013
4.129 ± 0.017
4.498 ± 0.025
9.102 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.232 ± 0.009
3.18 ± 0.015
6.153 ± 0.032
3.965 ± 0.021
7.032 ± 0.026
8.313 ± 0.033
5.511 ± 0.02
6.399 ± 0.022
1.387 ± 0.01
2.445 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here